Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,528,308 A→C 26.6% P87P (CCA→CCC)  argJ → Arginine biosynthesis bifunctional protein ArgJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,528,3080AC26.6% 43.1 / 6.0 58P87P (CCA→CCC) argJArginine biosynthesis bifunctional protein ArgJ
Reads supporting (aligned to +/- strand):  ref base A (29/12);  new base C (14/3);  total (43/15)
Fisher's exact test for biased strand distribution p-value = 5.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

AGCGTGGCTGGCGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCC‑GCTGCCGGTCGAAAAGATCGAAGGCGCGC  >  KT2440/1528177‑1528438
                                                                                                                                   |                                                                                                                                   
aGCGTGGCTGGCGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTACCGCCAACGCCGGCACCGGCGCGCCAGGc                                                                                                                                  >  8:39153/1‑135 (MQ=255)
aGCGTGGCTGGCGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGc                                                                                                                                  >  7:105280/1‑135 (MQ=255)
aGCGTGGCTGGCGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGc                                                                                                                                  >  7:111196/1‑135 (MQ=255)
aGCGTGGCTGGCGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGc                                                                                                                                  >  3:128515/1‑135 (MQ=255)
  ggTGGCTGGCGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGGGTGTACAGGGCACCGGGCGCTACCTGGTGCCCAACCCGGGTAACGCAAACGCCGGCCCCGGCGCGCCAGGCCt                                                                                                                                <  2:53513/134‑1 (MQ=255)
         ggcGTGTTGACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCGACCTGCTGACCAACACGGGGAACGCCAACGCCGGCCCCCGCGCCCCCCGCCGGgccccc                                                                                                                         >  1:153262/1‑135 (MQ=255)
         ggcGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGCTCCTGTCCAAACAGCGTGTAAAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCCGCCTGgccgcc                                                                                                                         >  2:120665/1‑135 (MQ=255)
         ggcGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCCCCGTGCGCTACCTGCTGACCACCACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGgccgcc                                                                                                                         >  3:213303/1‑135 (MQ=255)
         ggcGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGgccgcc                                                                                                                         >  2:25477/1‑135 (MQ=255)
         ggcGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCCGTGATCCTGTCCAAACAGCCTGTACAGGGCACCGTCCGCTTCCTGCTGCCCAACACGGGTAACGCCAACGCCCGCACCCGCGCGccaggccaggacccc                                                                                                                         >  1:249217/1‑130 (MQ=255)
            gtgtTCACCCGCACCGCCTTCTGCGCGGCGCCGGGGAGCCTGTCCAAACAGCGGGTACAGGGCACCGTGCGCTACCTGCTGACCAACAGGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGgccgccgcc                                                                                                                      <  7:309075/135‑1 (MQ=255)
               ttCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGCCGGgccgcccccccc                                                                                                                   >  1:254267/1‑132 (MQ=255)
                  ccccTCAACGCCTTCTGCGCGGCGCCGGTGATCCGGTCCAAACAGCGGGTCCCGGGCCCCGGGCGCTACCTGCTGACCACCCCGGGAAACCCCAACGCCGGCCCCGGCGCGCCAGGCCTGGCCGCCGCCGAgcg                                                                                                                 <  6:158072/133‑1 (MQ=255)
                  aCCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGCCAGGCCGCCCCCgcgccc                                                                                                                >  4:313823/1‑133 (MQ=255)
                         aCGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCCCCTgcg                                                                                                         >  1:157872/1‑135 (MQ=255)
                                  gcgcGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTgg                                                                                                >  7:276848/1‑135 (MQ=255)
                                    gcggcgCCGGTGAACCTGCCCAACCCGCGGGTACCGGGCACCGGGCGCTACCGGCTCCCAAACCCGGGAAACCCCACCGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGcc                                                                                              <  2:254267/135‑1 (MQ=255)
                                                     gcccAAACAGCGTGTACAGGCCACCGGGCGCTACCTGCTGACCACCACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTAcc                                                                             <  4:23251/133‑1 (MQ=255)
                                                     gTCCAAACCGCGTGTAGAGGGCCCCGTGCGCTACCTGCGGACCAACCCGGTTAACGCCAACGGCGGCAGCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTAcc                                                                             <  8:105280/135‑1 (MQ=255)
                                                       ccAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGCCCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTAcc                                                                             <  8:111196/133‑1 (MQ=255)
                                                         aaaCAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCCCCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGc                                                                          >  4:337074/1‑134 (MQ=255)
                                                          acccgCGGGTACAGGGCCCCGTGGGCTACCTGCTGACCAACACGGGTACCGCCACCGCCGGCCCCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTTCCGGCCg                                                                        <  2:133208/131‑1 (MQ=255)
                                                              gCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGCCCTGGCCGCCCCCGAGCGCACCTGCGGCAAGCTGGCCGCACAGGCTGGCGTACCGGCCGAGTc                                                                    >  5:389701/1‑135 (MQ=255)
                                                                  gtACCGGGCACCGGGCGCTACCTGCTGACCAACACGGGAACCGCCAACGCCGCCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTa                                                                <  5:202309/135‑1 (MQ=255)
                                                                  gtACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGCCTTGCCGCCGCCCAGCGCACCTGCGCCCAGCAGCCCGAACTGGCTGGCGTACCGGGCGCGTccgga                                                                >  1:386885/1‑131 (MQ=255)
                                                                  gtACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGCCTGGCCGCCGCCGCGCGCACCTGCGCCAACCTGGCCGAACAGGCAGGCGTACCGGCCGAGTCGGTa                                                                >  5:335541/1‑135 (MQ=255)
                                                                  gtACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCCCCTGCGCCAAGCTGGCCGCACTGGCTGGCGTACCGGCCGAGGCggga                                                                >  5:365463/1‑133 (MQ=255)
                                                                  gtACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCCCCTTCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTa                                                                >  5:133729/1‑135 (MQ=255)
                                                                  gtACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCCCCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGt                                                                 >  2:346930/1‑134 (MQ=255)
                                                                       gggCACCGTGCGCTACCTGCGGACCAACACGGGAAACGCCACCGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGcc                                                           <  2:113321/135‑1 (MQ=255)
                                                                          cACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGCCTGGCCGCCGCCGAGCGCCCCTGCGCCAAGCTGGCCGCACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGtt                                                        >  1:55404/1‑135 (MQ=255)
                                                                          cACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGCCCGGCCGCCGCCCCGCGCCCCCGCCCCCAGCAGGCCGCACAGGCAGGCGTACCGGCCGAGGCGGTGCGGCCggg                                                        >  1:187153/1‑133 (MQ=255)
                                                                          cACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGtt                                                        >  4:177460/1‑135 (MQ=255)
                                                                          cACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGACAGCACCAGCGCCACGCGGGCCGAACTGGCATGCCTACCGGCCGAGTAGGTACTGGCGtt                                                        >  2:235964/1‑135 (MQ=255)
                                                                          cACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCACGCCTGGCCGCCGCCGAGCGCCCCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTTCCGtt                                                        >  8:40993/1‑135 (MQ=255)
                                                                           aCCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTTCTGCCGGtc                                                       >  2:95845/1‑135 (MQ=255)
                                                                              gTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGGCCGGCCGGGCCCCCGCCCCGCGCCCCTGCGCCACGCGGGCCGCACTGGCTGGCGTACCGGCCCCGGCGGGACGGCCgcggggg                                                    >  3:368313/1‑129 (MQ=255)
                                                                              gTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGCCTGGCCGCCGCCGAGCGCACCGGCGCCAAGCTGGCCGCACTGGCTGGCGTACCCGCCGAGTCGGTACTGCCGGTCTcc                                                    >  3:145063/1‑135 (MQ=255)
                                                                                gcgcTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCCCCAGCGCCCAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGGCGGTACTGCCGGTCTcccc                                                  >  5:22788/1‑133 (MQ=255)
                                                                                gcgcTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGCACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCAc                                                  >  1:1961/1‑135 (MQ=255)
                                                                                     aCCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCCGGCCAGGCCGCCGCCGCGCGCACCGGCGCCAAGCTGGCCGACCTGGCTGGCGTACCGGCCGCGTCGGTACTGCCGTTCTCCACCGGCg                                             >  2:204327/1‑135 (MQ=255)
                                                                                              ccAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCACCTGCGCCCAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCg                                    >  4:273342/1‑135 (MQ=255)
                                                                                                     gggTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCCCCTGCGCCCAGCTGGCCGCACAGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGGGCTTTGCGCGccccc                             >  3:65136/1‑132 (MQ=255)
                                                                                                     gggTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCC‑GCt                            >  7:224957/1‑135 (MQ=255)
                                                                                                          acgccaacgccGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGGTTGGGGCGCC‑gcgggcgg                       >  3:276412/1‑135 (MQ=255)
                                                                                                           cgccaacgccGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCC‑GCTGCCGGt                      <  2:369008/135‑1 (MQ=255)
                                                                                                               aacgccGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGCGCC‑GCTGCCGGTCGa                   >  6:178412/1‑134 (MQ=255)
                                                                                                               aacgccGGCACCGGCGCGCCAGGCCAGGCCGCCGCCGCGCGCCCCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTTGCGAGCC‑GCTGCCGGTCGa                   >  2:275913/1‑134 (MQ=255)
                                                                                                                        aCCGGCGCGCCCGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCC‑GCTGCCGGTCGAAAAGATCGaa         <  3:177460/135‑1 (MQ=255)
                                                                                                                        aCCGGCGCGCCAGGCCGGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCC‑GCTGCCGGTCGAAAAGATCGaa         <  3:39679/135‑1 (MQ=255)
                                                                                                                         cgggCGCGCCCGGCCGGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCC‑GCTGCCGGTCGAAAAGATCGAAg        <  3:10631/133‑1 (MQ=255)
                                                                                                                         ccGGCGCGCCCGGCCTGGCCGCCGCCGCGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGCGTCGGTACTGCCGTTCTCCACCGGCGGGGTTGGGGCGCC‑GCGGCCGGGCGCAAAGATCGCAg        >  1:248651/1‑135 (MQ=255)
                                                                                                                         ccGGCGCGCCCGGCCGGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCC‑GCTGCCGGTCGAAAAGATCGAAg        <  8:216935/135‑1 (MQ=255)
                                                                                                                         ccGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCACCTGCGCCAAGCTGGCCGCACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGCGTGGCGCGCC‑GCGGCCGGGCGAAAAGCTCGAAg        >  5:185500/1‑135 (MQ=255)
                                                                                                                         ccGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGCACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGGTCTCCCCCGGCGGGGTGGGCGGGCCGGCGGCCGGCGGAAAAgtgggag         >  1:187576/1‑129 (MQ=255)
                                                                                                                         ccGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGGTTGGCGCGCC‑GCGGCCGGGCGAAAAGATCGCAg        >  7:125546/1‑135 (MQ=255)
                                                                                                                         ccGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGGGGTTGGCGGGCC‑GCGGCCGGTCGCAAAGATCGAAg        >  3:125266/1‑135 (MQ=255)
                                                                                                                               cgcCAGGCCTGGCCGCCGCCGCGCGCCCCTGCGCCAAGCTGGCCGCACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGGTTTGGGCGCC‑GCGGGCGGGCGGAAAGATCGCACgcgcgc  >  6:273170/1‑135 (MQ=255)
                                                                                                                               cgcCAGGCCTGGCCGCCGCCGCGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGGTTGGCGAGCC‑CCGGGCGGGCGGAAAGATCGAAGgcgcgc  >  8:222398/1‑135 (MQ=255)
                                                                                                                                   |                                                                                                                                   
AGCGTGGCTGGCGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGGTAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCC‑GCTGCCGGTCGAAAAGATCGAAGGCGCGC  >  KT2440/1528177‑1528438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑