| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 1,585,043 | T→G | 37.2% | intergenic (‑94/‑109) | rhaS_1 ← / → CJ019_01492 | HTH‑type transcriptional activator RhaS/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 1,585,043 | 0 | T | G | 37.2% | 14.5 / 3.2 | 33 | intergenic (‑94/‑109) | rhaS_1/CJ019_01492 | HTH‑type transcriptional activator RhaS/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (7/13); new base G (4/8); total (12/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGAT‑CAGGCAATCGAC‑ATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCC > KT2440/1584912‑1585176 | aGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTTCCAGAAACAGCCCCGCTGcc > 4:120754/1‑134 (MQ=255)aGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCa < 5:101403/135‑1 (MQ=255)aGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCa > 8:301094/1‑135 (MQ=255) gggTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCAGATACAGCCCCGCGGCAGGCT‑CAg > 4:249069/1‑135 (MQ=255) gggtccgggGGACGTGGCGGGGGTCATGATGGCTTTCGTCTGCTGACCGACGCAGCATCGGTTGGGGCAACGCCGGGCGCTAGTCTAAAGCTCCAGGTGGATGGCCTGATCCTGCCGCGCTGCTGGAT‑CTGGCaa < 2:82622/134‑1 (MQ=255) tgcggtGGACGTGGCGGGGGGCATGATGGCTTCCCTCTGCTGCCCGACGCAGCATAGGTTTGGGCAAAGCCGGGCGCTAGGCGAAAGCTGCATGTGGATTGCCTCATCCTGCCGCGCGGCAGGAT‑CAGGCAATCg < 8:33413/133‑1 (MQ=37) tcggtGGACGTGGCGGTGGTCATGATGCCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTTCCTGATACTCCCCCGCGGCAGGGA‑CAGGGaaagca > 6:69545/1‑131 (MQ=255) cggtGGACGTGGCGGGGGCCATGATGGCTTTCCTCTGCTCACCCACGCAGCATCGGTTGGGGCAAACACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGAT‑CAGGCAATCGAc < 3:110766/135‑1 (MQ=255) ggtGGCCGGGGCGGTGGCCATGATGGCTTTCCGCTGCTGCCCGCCCCAGCATAGGTTTGGGAAAACACGGGCGCTAGTCGAAACCGGCATGGGGATGGCCTGAACCTCCCGCGCTGCAGCAG‑CAGGCAATCGAc < 2:83192/134‑1 (MQ=255) gACGTGGGGGGGGTCATGATGGCTTTCCTCTGCGGACCCACGCAGCATAGGTTTGGCCAAAGCCGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGAGCCTGCCGCGCTGCAGGAT‑CAGGCAATCGAC‑ATGCa < 2:293318/135‑1 (MQ=255) gCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATGCTGCCGCGCTGCATGAT‑GCTGCAATTGAC‑ATGCAGTTAtt > 8:188367/1‑134 (MQ=255) gCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTACCGCGCTGCAGGAT‑CAGGCAATCg < 6:156062/122‑1 (MQ=255) gCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTACCGCGCTGCAGGAT‑CAGGCAATCg > 5:156062/1‑122 (MQ=255) atgatgGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCGTTTCCGGCCGCGCCGCCGGCT‑ACGGCAATCGAC‑AAGGAGTTATTGCCGAAcgc > 8:351832/1‑135 (MQ=255) agggCTTCCCTCTGCTGACCGACGCAGCATAGGTTGGGGCAAAGCCGGGCGCTAGGCGAAAGCTGCAGGGGGATGGCCTGACCCGCCCGCGCGGCAGGAA‑CAGGCAATCGAC‑ATGCAGTAATGGCCTAAcgcgcc < 3:381410/133‑1 (MQ=255) ccTCTGCTGCCCGACGCAGCATAGGTTTGGGCAACCCCGGGCGCTAGGCGAACGCTGCATGTGCATTGCCTGATCCGGCCGCGCGGCAGGAT‑CAGGCAATCGAC‑ATGCAGTAATGGCCTAACGCGCCGCGCgg < 2:68149/133‑1 (MQ=37) tgACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGAT‑CAGGCAATCGAC‑ATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAgcg < 8:373928/133‑1 (MQ=255) tcaCCGAGGCACAATTGGTTTGGCCAACGCCGCGCGATAGGCGAACCCGGCATGTGGATTGCCAGATCCTGCCGCGCGGCCGGAA‑CAGCAAATCGAC‑ATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAgcg < 2:74634/131‑1 (MQ=255) gACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGGCTGGTGCAGCCCCGCGGCCCGCTACCGCAAACCGCC‑ATG‑AGGAATGGCCGAAACCGCCGCGCGGGTGGGCGCCAAAcc > 6:100383/1‑135 (MQ=255) agcaTAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCGGATACATCCGCGCGGGCGGCT‑AAGGGCATCGCCAATGACGTAATGG‑CAAACGCCCCCCCCGGGGGTGCGCCCAAccattgg > 4:346693/1‑130 (MQ=255) gCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCCGCCGCGCTGCAGGAT‑CAGGCAATCGAC‑ATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtc > 6:383498/1‑135 (MQ=255) gCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCAGATCCTGCCGCGCTTCAGGGT‑CAGGGAATCGAC‑ATGCTGTAATTGCCGAACGCGCCGCGCGGGGGAGCGCCGAAACTTTGCAGCCTGGCGTTtc > 8:331867/1‑135 (MQ=255) aGACGGGCGCTAGTCTAAAGCGGCATGTGGTTGGCCGGATCGTGCGGCGGTGAAGTTT‑ATGGAAATCGAC‑ATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtccgtc < 2:364166/135‑1 (MQ=255) aGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGACCCTGCCGCGGGGCAGGAT‑CAGGCAATCGAC‑ATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtccgtc < 2:55889/135‑1 (MQ=255) aGACGGGCGCTAGGCGAACGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGAT‑CAGGCAATCGAC‑ATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtccgtc < 3:201277/135‑1 (MQ=255) cTAGGCGAACGCTGCATGGGGATTGCCTGAGCCTGCCGCGCGGCAGGAT‑CAGGCAATCGAC‑ATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCtt < 4:250790/135‑1 (MQ=38) tgaggAACGCTGCTTGGGGTTTGCCGGAGCGGGCGGCGCGGCAGGAT‑CTGGAAATCGAC‑ATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCa < 5:140122/131‑1 (MQ=255) cGAAAGGTGCTTGTGGATTGCGTGATCCTGCCGCGTTGCTGTAT‑CTGGAATTCGAC‑ATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCGGCCTGCTTCAAgg < 7:27244/135‑1 (MQ=255) aaaGCTGCTTGTGGATTGCCTGATCCTGCCGCGCTGCAGTAT‑ATGGAAATCGAC‑ATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGgag < 7:57648/135‑1 (MQ=255) attggggATTGCCTGAGCCGCCCGCGCGGCCGTTT‑CAGGAAATCGAC‑ATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGc < 3:249069/130‑1 (MQ=255) aggtgGATTGCATGATCCTCCCGCGCTGCAGTAT‑CAGGCAATCGAC‑ATGCAGTAATGGCATAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGc < 6:106639/132‑1 (MQ=255) taTCCTGCCGCGCTGCAGTAT‑CTGGAAATCGAC‑ATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAaac < 7:351832/134‑1 (MQ=255) cgcgcgGCAGCAT‑CAGTAAATCGAC‑ATGCAGTAATGGCCTGACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTc < 5:323813/135‑1 (MQ=255) gcgcTGCAGGAT‑CAGGCAATCGAC‑ATGCAGTAATGGCCTAACGCGCCGCGCGTGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCGTCAAGGAGATTGTGCAATGCCCCTTAAACAACCGGTca > 1:323418/1‑135 (MQ=255) ctgctgGAT‑CAGGCAATCGAC‑ATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTcaccc < 1:269940/135‑1 (MQ=255) | AGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGAT‑CAGGCAATCGAC‑ATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCC > KT2440/1584912‑1585176 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |