Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 2,131,359 T→G 32.1% R135R (CGT→CGG)  yadG → putative ABC transporter ATP‑binding proteinYadG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,131,3590TG32.1% 57.6 / 7.4 49R135R (CGT→CGG) yadGputative ABC transporter ATP‑binding proteinYadG
Reads supporting (aligned to +/- strand):  ref base T (12/20);  new base G (15/2);  total (27/22)
Fisher's exact test for biased strand distribution p-value = 8.17e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAAGACCTTCGACATCGTCGTGACCCAGGCCGGGTACTACGGCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCC  >  KT2440/2131232‑2131489
                                                                                                                               |                                                                                                                                  
aaaaGACCTTCGACATCGTCGTGACCCAGGCCGGGTACTACGGCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGt                                                                                                                             <  2:149702/135‑1 (MQ=255)
 aaaGACCTTCGACATCGTCGAGACCCAGGCCGGGTACTACGGCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTc                                                                                                                            <  1:84813/135‑1 (MQ=255)
   aGACCTTCGACATCGTCGTGACCCAGGCCGGGTACTACGGCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCcg                                                                                                                          <  6:143011/135‑1 (MQ=255)
      ccTTCGACATCGTCGTGACCCAGGCCGGGTACTACGGCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCcggcg                                                                                                                       <  4:160509/135‑1 (MQ=255)
       cTTCGAGATGGTCGTGACCCAGGCCGGGTACTACGGCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGACTGTGGGAGAAACGTGATGTGCAGTCGCGTTCGTTGTCcggcgg                                                                                                                      <  3:56593/135‑1 (MQ=255)
         tCGACATCGTCGTGACCCAGGCCGGGTACTACGGCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGt                                                                                                                             >  1:70443/1‑126 (MQ=255)
         tCGACATCGTCGTGACCCAGGCCGGGTACTACGGCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGt                                                                                                                             <  2:70443/126‑1 (MQ=255)
            aCATCGTCGTGACCCAGGCCGGGTACTACGGCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGa                                                                                                                 <  2:341720/135‑1 (MQ=255)
                  tcgtGACCCAGGCCGGGTACTACGGCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAgcggc                                                                                                           <  7:61299/135‑1 (MQ=255)
                   cgtGACCCAGGCCGGGTACTACGGCATCCCGCCTAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGGTCGGTGTCCGGCGGCAGGAAgcgggg                                                                                                          >  2:171411/1‑133 (MQ=255)
                          cAGGCCGGGTACTACGGCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGGTCGTTTTACGGCGGCCTGAACCGGCGCCggatg                                                                                                   >  8:235754/1‑135 (MQ=255)
                              ccGGGTACTACGGCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACACACGTGATGTGCAGTCGCGGTGGTTGTCAGGGGGCCTGAAGAGGCGCCggattgttt                                                                                               >  1:51507/1‑130 (MQ=255)
                               cGGGTACTACGGCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGGTGGGTGTCCGGGGGCATGCAGCGGCGCCGGCTGGTTGc                                                                                              >  3:35811/1‑135 (MQ=255)
                                  gTACTACGGCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCcg                                                                                           <  5:222980/135‑1 (MQ=255)
                                      tacGGCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCgcgc                                                                                        <  8:113512/134‑1 (MQ=255)
                                         ggCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTCCCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGGTCGTTGTCCGGCGGCTTGAAGCGGCGCCGGAGGAGTGCCcgccccttt                                                                                    >  2:296719/1‑129 (MQ=255)
                                           cATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGGTCGGTGTCCGGCGGCCTGACGCGGCGCCGGATGCTTGCCCgcgccgtgct                                                                                  >  7:322634/1‑129 (MQ=255)
                                              cccGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGGTCGTTGTCCGGCGGCATGAAGCGGCGCCGGATGATTGGCCGCGCCTTgttgca                                                                               >  4:365910/1‑130 (MQ=255)
                                                cGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACg                                                                             <  7:66249/135‑1 (MQ=255)
                                                    cAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAAcc                                                                         <  7:251227/135‑1 (MQ=255)
                                                              aaGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCACATTGATCCACGAACCGCGCctgctg                                                               >  4:89410/1‑135 (MQ=255)
                                                              aaGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGGTGGTTGTGCGGCGGCATGAAGCGGCGCCTGATGCTTTCCCGCGCATTGATCCACGCACCGCGCcggcgg                                                               >  5:194297/1‑133 (MQ=255)
                                                              aaGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGGTGGGTGTCCGGGGGGATGAAGCGGCGCCTGAGTGTTGGCCGCGCAGTTATCCACGGACCCCGCcagcgg                                                               >  6:388569/1‑133 (MQ=255)
                                                                   gcgcgcCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGGTGTCCGGCGGCATGAAGCGGCGCCGGATGATTGCCCGCGCATTTATCCCCGCACCGCGCCTGCGGATCCt                                                          >  8:244379/1‑135 (MQ=255)
                                                                        ccGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGGTCGTTGTCCGGCGGCATGAAGCGGCGCCTGCTGATTGCCCGCGCATTGATCCACGCACCGCGCCTGCTGATGCTGGAc                                                      >  4:166912/1‑134 (MQ=255)
                                                                         cGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGGTCGTTGTCCGGGGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGCACCGCGCCTGCTGATCCTGGTCGa                                                    >  8:203673/1‑135 (MQ=255)
                                                                                tACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGACGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGATGATCCTGGACGAACCCAcc                                             <  6:189130/135‑1 (MQ=255)
                                                                                   cTGACGCAGCGGGGCCTGTGGGACAAACGTTAGGTGCAGGCGCGTTCGTGGGCCGGCGGCATGACGCGGCGCCTGATGATAGCCCGCGAATTGACCCACGAACCGCGCCTGCCGATCCTGGACGAACCCAccgcc                                          <  2:323295/135‑1 (MQ=255)
                                                                                       cTCAGCGGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGGTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCgg                                        <  5:255276/133‑1 (MQ=255)
                                                                                        tCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCGGATGATTGCCCGCGCATTGTTCCACGAACCGCGCCTGCTGATCCAGGACGAACCCCCCCCCggggg                                     >  6:161774/1‑134 (MQ=255)
                                                                                        tCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGGTGTCCCGCGGCATGAAGCCGCGCCTGCTGATTTGCCGCGCATTGATCACCCAACCCCGCCGGCTGATACAGGCCGAACCCCCCCCCCGGGt                                     >  4:54750/1‑135 (MQ=255)
                                                                                        tCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGGTGGTTGTCCGGCGGCATGAAGCGGCGCCGGCTGATTTCCCGCGCAATGATCCCCGAACCCCGCCAGCGGATACGGGACGAACCCCCCCCCggggg                                     >  1:311258/1‑134 (MQ=255)
                                                                                          aGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTAGTTGTCCGGCGGCAAGAACCGGCGCCTGAAGATTTCCCGCGCATTGCTGCACGCACCGCGCCTGCTGATCCTGGACGAACCCAccgcccgcggg                                    >  6:108277/1‑128 (MQ=255)
                                                                                           gCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTgg                                   <  2:51507/134‑1 (MQ=255)
                                                                                             tGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTGGTTGTACGGCGGCATGAACCGGCGCCAGATGTATGACCCCGCATTGAACCACGCACCCCCCCAGCTGATCCAGGACGAAAACACCGCCCGGGTGGat                                 >  4:331765/1‑134 (MQ=255)
                                                                                             tGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCGGGGGGCCTGAAGCGGCGCCTGTTGATTTGCCGCCCAATGCTCCACCAACCGCCCCTGCTGCTGCAGGACCAACCCACCGCCCGGGGGGat                                 >  8:387407/1‑134 (MQ=255)
                                                                                             tGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGGTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTTCCCGCGCATTGATCCACGCCCCGCGCCGGCTGCTCCAGGTCGAACCCACCGCCCGGGTGGat                                 >  6:108879/1‑134 (MQ=255)
                                                                                             tGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGGTCGGTGTGCTGCGGCCTGAAACGGCGCCCTAAGTTTGCCCGCCCAGATATCCACCAACCGCGCCCCCGGATACAGGACGCACACACACCCGCGGtcgta                                 >  6:311870/1‑130 (MQ=255)
                                                                                               ggCCTGTGGGACAAACGTGATGTGCAGTCGCGTTAGTTGTACGGCGGCATTAATACGCGCCCGATGATTTCCCGCGCATTTATACAAGAACCGCGCCAGCTGATCCAGGAAGAACCCCCCCCCCGGGGGGat                                 >  4:59595/1‑132 (MQ=255)
                                                                                               ggCCTGTGGGACAAACGTGATGTGCAGTCGCGGTGGGTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCCCGCCGGCTGATACCGGCCGAACCCACCCCCGGGGTGGaaat                               >  2:40959/1‑134 (MQ=255)
                                                                                                  cTGTGGGACAAACGTGATGTGCAGTCGCGTTGGTTGTCCGGCGGCATGAAGCGGCGCCGGATGATTGCCCGCGCATTGATACACGAACCCCGCCGGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATagag                           >  4:37865/1‑135 (MQ=255)
                                                                                                   tgtgGGACAAACGTGATGTGCAGTCGCGTTAGTTGTCCGGCCGCAAGAATACGCCCCAGTTGTTTGCCCCCGCATTGATAACAGAAACACGGCTGCGGATACAGGACGAAAACAACCCCGGGGTGGataaatacc                          >  4:335279/1‑129 (MQ=255)
                                                                                                      gggcccaaCGGGATGGGCAGGCGCGGTCGTGGGCCGGCGGCAGGACGCGGCGCCTGATGATGGCCCGCGCATGGACCCCCGACCCGCGCCTGCTGACCCTGGACGACCCCACCGCCGGGGTGGATATCGAGCTgc                       <  3:21374/129‑1 (MQ=255)
                                                                                                         aCAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGcgcc                    <  7:63700/135‑1 (MQ=255)
                                                                                                                   ggtgGAGTGGCGTTCGTTGTCGGGCTGCATGAAGCGGCGCCTGATGATTGCCCGCGCTTTGACCCAGGAACCGCGCCTGCTTATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCGCGAtgtg          <  3:335279/134‑1 (MQ=255)
                                                                                                                     tgCAGTCGCGTTCGTTGTGCGGCGGCATGAAGCGGCGCCTGATGATTGCCCCCGCAATGCTCCCCCCACCGCGCCGGCTGATCCTGGCCGAACCCACCCCCGGGGTGGATATCGAGCTGCGCCGCTCGCTGTGGa        >  6:145395/1‑135 (MQ=255)
                                                                                                                      gCAGGCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAg       <  8:322634/135‑1 (MQ=255)
                                                                                                                           cgcgTGCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTcc  <  2:311258/135‑1 (MQ=255)
                                                                                                                           cgcgTGCGTGGCCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGACCCACGAACCGCGCCGGCTGATCCGGCCCGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTcc  <  5:87796/135‑1 (MQ=255)
                                                                                                                               |                                                                                                                                  
AAAAGACCTTCGACATCGTCGTGACCCAGGCCGGGTACTACGGCATCCCGCCCAAGCTGGCCAAGGAGCGCGCCGAGCAGTACCTGACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCC  >  KT2440/2131232‑2131489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑