| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 3,275,333 | T→G | 17.0% | A30A (GCA→GCC) | CJ019_03079 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,275,333 | 0 | T | G | 17.0% | 27.2 / 4.6 | 29 | A30A (GCA→GCC) | CJ019_03079 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (2/22); new base G (3/2); total (5/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.43e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-02 | |||||||||||
CCAGTGTTCGAGGCCGGCCTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGGTGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTC > KT2440/3275201‑3275460 | cgaGTGTTCGAGGCCGGCCTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGc < 8:292253/133‑1 (MQ=255)ccAGTGTTCGAGGCCGGCCTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGc < 5:251873/135‑1 (MQ=255)ccAGTGTTCGAGGCCGGCCTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGGGc > 7:201042/1‑135 (MQ=255)ccAGTGTTCGAGGCCGGCCTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCCTgg > 3:246025/1‑134 (MQ=255)ccAGTGTTCGAGGCCGGCCTGTTCGCCGGCGGGGGCGTGGCAACGAACGCAATGCGCCAGGCCTTCGGGAGCCAGGGCCAAGGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGc < 1:96165/135‑1 (MQ=255) cAGTGTTCGAGGCCGGCCTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGc < 5:158950/134‑1 (MQ=255) cAGTGTTCGAGGCCGGCCTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGc < 3:227626/134‑1 (MQ=255) aGTGTTCGAGGCCGGCCTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGGGCAg > 5:114644/1‑135 (MQ=255) tCGAGGCCGGCCTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTAGCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCAcc < 2:305904/135‑1 (MQ=255) tCGAGGCCGGCCTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCATCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCAcc < 2:142322/135‑1 (MQ=255) aGGCCGGCCTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGCCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCa < 5:343979/135‑1 (MQ=255) ggccTGTGGGCCGGGGGGGGCTTGGCACCTAACGAAATGCGCCTGGCCTTCGGTAGCCCGGGCCAAGGTCAGTTGCCAGCCCGGGTTGCCGACATCGATCAGGCCGCCATCGCGGGGTGCAGCCACCTCACggtg < 8:150030/135‑1 (MQ=255) ggccTGTGCGCCGGCGGGGGCTTGGCAACGAACTCAAGGCGCCAGGCCTGCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCAGCGCGGCGTGCAGCCACCTCAAggtg < 8:244817/135‑1 (MQ=255) gCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGGGGCGCCCCCCCCAAGGGGTGGACGcc > 8:158084/1‑135 (MQ=255) cGTCGGGGGCTTGGCACCTACCGAAATGCGCCTGGCCTTCGGTATCCAGGGCAACCGTCAGTTGCCAGCCCTGGATGCCGACATCTCTCAGTCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGGTGCACGCCaa < 4:176156/135‑1 (MQ=255) cAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGGGCAGCCACCTCAAGGTGGTGCACGCCAAGGGCCACATTgcgc < 6:114644/135‑1 (MQ=255) accaacgCAATGCGCCAGGCCTTCGGTATCCAGGGCAAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGGGCAGCCACCTCAAGGTGGTGCACGCCAAGGGCCACATTGCGCAg < 3:343475/132‑1 (MQ=255) gaacgCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGGTGCACGCCAAGGGCCACATTGCGCAGGc < 8:59223/135‑1 (MQ=255) ccAGGGCCAACGTCAGTTGCCAGCCCGGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGGGGTGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTg < 4:333458/135‑1 (MQ=255) ccAGGGCCAACGGCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGGAGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTg < 4:202394/135‑1 (MQ=255) gCCAAGGTCCGTTGCCAGCCGTGGATGCCGACTTCGATCAGGCCGCCATGGCGGGGTGCAGCCACCTCAAGGTGGGGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGt < 1:216628/135‑1 (MQ=255) gCCAACGCCAGTTGCCAGCCCTGGATGCCGACATCGATCAGCCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGGTGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGt < 3:251865/135‑1 (MQ=255) ggcAGTTGCATGCCCTGGTTGCCGACAGCGATCAGGCCGCCATCGCGGCGTGCAGCAACCTCACGGTGGTGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTgg < 6:314996/133‑1 (MQ=255) tCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGc < 5:277171/52‑1 (MQ=255) tCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGc > 6:277171/1‑52 (MQ=255) ttGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCAGCGCGGCGTGCAGCCACCTCAAGGTGGTGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCaa < 2:371268/135‑1 (MQ=255) aTCAGGCCGCCATGGCGGCGTGCAGCCACCTGACGGGGGTGCACGCCAAGGGCCACATTGCGCTGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCATAAATTCGCCGGCGTGTTgg < 7:68973/135‑1 (MQ=255) aGGCCGCCATCGCGGCGTGCAGCCACCTCACGGTGGTGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGgcgc < 8:215467/135‑1 (MQ=255) cgcgGCGTGCCCCCACCTCAGGGTGGGGCAGGCCAAGTGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGAtctc < 5:124822/135‑1 (MQ=255) | CCAGTGTTCGAGGCCGGCCTGTTCGCCGTCGGGGGCTTGGCAACGAACGCAATGCGCCAGGCCTTCGGTATCCAGGGCCAACGTCAGTTGCCAGCCCTGGATGCCGACATCGATCAGGCCGCCATCGCGGCGTGCAGCCACCTCAAGGTGGTGCACGCCAAGGGCCACATTGCGCAGGCACTGGTAGACATCCCGGGCGTTCGGTGAACGTCTGTGGGTCAAGATCAGAAATTCGCCGGCGTGTTGGCGCTGATGATCTC > KT2440/3275201‑3275460 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |