Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	3,813,093	A→G	6.7%	S43G (AGC→GGC)	CJ019_03616 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	3,813,093	0	A	G	6.7%	189.7 / 3.9	60	S43G (AGC→GGC)	CJ019_03616	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (17/39);  new base G (2/2);  total (19/41)
Fisher's exact test for biased strand distribution p-value = 5.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

TTTCCCCATGAAATCACTGCTTTACGCCGTAACCTTATTATTTTCCTTTACCCTCCCCGCTCTGGCCAACCCGCCGGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGACGCTGCCTCGTGTGGTTACCAGACACGCAAGCAGCAGAACCGTGCCGAACGCACCGAGTGGGAA  >  KT2440/3812960‑3813227                                                                                                                                      |                                                                                                                                       tttCCCCATGAAATCACTGCTTTACGCCGTAACCTTATTATTTTCCTTTACCCTCCCCGCTCTGGCCAACCCGCCGGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCca                                                                                                                                        <  2:187785/134‑1 (MQ=255) tttCCCCATGAAATCACTGCTTTACGCCGTAACCTTATTATTTTCCTTTACCCTCCCCGCTCTGGCAAACCCGCCGGCCACATTCACCGAAGCCAAGCTGGTTGCCAAGCAGAAGGTCTACCTGGACCAGGCca                                                                                                                                        >  5:179958/1‑134 (MQ=255)   tCCCCATGAAATCACTGCTTTACGCCGTAACCTTATTATTTTCCTTTACCCTCCCCGCTCTGGCCAACCCGCCGGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCcagca                                                                                                                                     >  3:307973/1‑135 (MQ=255)      ccATGAAATCACTGCTTTACGCCGTAACCTTATTATTTTCCTTTACCCTCCCCGCTCTGGCCAACCCGCCGGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTg                                                                                                                                  >  7:172041/1‑135 (MQ=255)         tGAAATCACTGCTTTACGCCGTAACCTTATTATTTTCCTTTACCCTCCCCGCTCTGGCCAACCCGCCGGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCa                                                                                                                               <  7:268062/135‑1 (MQ=255)         tGAAATCACTGCTTTACGCCGTAACCTTATTATTTTCCTTTACCCTCCCCGCTCTGGCCAACCCGCCGGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCa                                                                                                                               <  4:386057/135‑1 (MQ=255)         tGAAATCACTGCTTTACGCCGTAACCTTATTATTTTCCTTTACCCTCCCCGCTCTGGCCAACCCGCCGGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCa                                                                                                                               <  4:179334/135‑1 (MQ=255)         tGAAATCACTGCTTTACGCCGAAACCTTATTATTTTCCTTTACCCTCCCCGCTCTGGCCAACCCGCCGGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGGGCCa                                                                                                                               <  1:85904/135‑1 (MQ=255)            aaTCACTGCTTTACGCCGTAACCTTATTATTTTCCTTTACCCTCCCCGCTCTGGCCAACCCGCCGGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATgg                                                                                                                            <  2:335363/135‑1 (MQ=255)            aaTCACTGCTTTACGCCGTAACCTTATTATTTTCCTTTACCCTCCCCGCTCTGGCCAACCCGCCGGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATg                                                                                                                             <  8:129654/134‑1 (MQ=255)              tCACTGCTTTACGCCGTAACCTTATTATTTTCCTTTACCCTCCCCGCTCTGGCCAACCCGCCGGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGc                                                                                                                          <  1:25530/135‑1 (MQ=255)              tCACTGCTTTACGCCGTAACCTTATTATTTTCCTTTACCCTCCCCGCTCTGGCCAACCCGCCGGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGc                                                                                                                          <  3:268741/135‑1 (MQ=255)              tCACTGCTTTACGCCGTAACCTTATTATTTTCCTTTACCCTCCCCGCTCTGGCCAACCCGCCGGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGc                                                                                                                          <  3:255189/135‑1 (MQ=255)              tCACGGCTTTACGCCGTAACCTTATTATTTTCCTTTACCCTCCCCGCTCTGGCCAACCCGCCGGCCACCTTCACCGAAGCCAAGGTGGTGGCCTAGCAGAAGGGCTACCTGGACCAGGCCAGCAGTGCCATGGGc                                                                                                                          <  1:120939/135‑1 (MQ=255)                       tACGCCGTAACCTTATTATTTTCCTTTACCCTCCCCGCTCTGGCCAACCCGCCGGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTAc                                                                                                                 >  6:9024/1‑135 (MQ=255)                           ccGTAACCTTATTATTTTCCTTTACCCTCCCCGCTCTGGCCAACCCGCCGGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCg                                                                                                             <  6:136261/135‑1 (MQ=255)                               aaCCTTATTATTTTCCTTAACCCTCCCCGCGCTGGCCAACCCGCCGTCAACCTTCACCGAAGCCAAGGTTGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTg                                                                                                         <  2:123469/135‑1 (MQ=255)                                 ccTTATTATTTTCCTTTACCCTCCCCGCTCTGGCCAACCCGCCGGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCGGCAGTGCCATGGGCGACCTGTACTGCGGTTGc                                                                                                        >  6:161039/1‑134 (MQ=255)                                 ccTTATTATTTTCCTTTACCCTCCCCGCTCTGGCCAACCCGCCGGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGc                                                                                                        >  3:196134/1‑134 (MQ=255)                                       tattTTCCTTTACCCTCCCCGCTCTGGCCAACCCGCCGGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGa                                                                                                 >  7:228359/1‑135 (MQ=255)                                         ttttCCTTTACCCTCCCCGCTCTGGCCAACCCGCCGGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGAcc                                                                                               >  8:388825/1‑135 (MQ=255)                                          tttCCTTTACCCTCCCCGCTCTGGCCAACCCGCCGGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCt                                                                                              <  8:351276/135‑1 (MQ=255)                                             ccTTTACCCTCCCCGCTCTGGCCACCCCGCCGTCCACCTTCACCGAATCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCAATGGGCGACCTGTACTGCGGTTGCAAATGGACCtgt                                                                                            <  5:209919/134‑2 (MQ=255)                                             ccTTTACCCTCCCCGCTCTGGCCAACCCGCCGGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCtggg                                                                                           >  6:294833/1‑135 (MQ=255)                                              cTTTACCCTCCCCGCTCTGGCCAACCCGCCGGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCGGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCtgggt                                                                                          >  3:65104/1‑135 (MQ=255)                                              cTTTACCCTCCCCGCTCTGGCCAACCCGCCGGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCtgggt                                                                                          >  1:386464/1‑135 (MQ=255)                                               tttACCCTCCCCGCTCTGGCCAACCCGCCGGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCtgggtg                                                                                         >  6:97413/1‑135 (MQ=255)                                               tttACCCTCCCCGCTCTGGCCAACCCGCCGGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCtgggt                                                                                          <  4:91553/134‑1 (MQ=255)                                                           gCTCTGGCCAACCCGCCGGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTggc                                                                             >  8:136454/1‑135 (MQ=255)                                                                     aCCCGCCGGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCg                                                                   <  7:135974/135‑1 (MQ=255)                                                                       gcgccgGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGAc                                                                 <  1:16878/134‑1 (MQ=255)                                                                       ccgccgGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGAc                                                                 <  6:193601/135‑1 (MQ=255)                                                                         gccgGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGAc                                                                 >  4:323452/1‑133 (MQ=255)                                                                         gccgGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGAc                                                                 <  3:323452/133‑1 (MQ=255)                                                                          ccgGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGACGCt                                                              <  8:228359/135‑1 (MQ=255)                                                                            ggCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGACGCTGc                                                            <  1:36699/135‑1 (MQ=255)                                                                              ccACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTg                                                                                                         >  2:111650/1‑88 (MQ=255)                                                                              ccACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATCGGCGACCTGTACTGCGGTTg                                                                                                         <  1:111650/88‑1 (MQ=255)                                                                                       ccGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGACGCTGCCTCGTGTGGtt                                                 >  6:101396/1‑135 (MQ=255)                                                                                          aaGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGACGCTGCCTCGTGTGGTTAcc                                              <  5:294833/135‑1 (MQ=255)                                                                                               aaGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGACGCTGCCTCGTGTGGTTACCAGaca                                         >  5:191187/1‑135 (MQ=255)                                                                                                aGGTGGTGGCCTAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGACGCTGCCTCGTGTGGTTACCAGacac                                        <  8:45065/135‑1 (MQ=255)                                                                                                aGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCGGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGACGCTGCCTCGTGTGGTTACCAGacac                                        <  4:65104/135‑1 (MQ=255)                                                                                                aGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCGGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGACGCTGCCTCGTGTGGTTACCAGacac                                        <  5:161039/135‑1 (MQ=255)                                                                                                aGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGACGCTGCCTCGTGTGGTTACCAGacac                                        <  8:296387/135‑1 (MQ=255)                                                                                                aGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGACGCTGCCTCGTGTGGTTACCAGacac                                        <  5:9024/135‑1 (MQ=255)                                                                                                aGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGACGCTGCCTCGTGTGGTTACCAGacac                                        <  5:34759/135‑1 (MQ=255)                                                                                                aGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGACGCTGCCTCGTGTGGTTACCAGacac                                        <  5:174729/135‑1 (MQ=255)                                                                                                aGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGACGCTGCCTCGTGTGGTTACCAGacac                                        <  2:386464/135‑1 (MQ=255)                                                                                                aGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGACGCTGCCGCGTGGGGTTACCAGacac                                        <  4:179287/135‑1 (MQ=255)                                                                                                aGGTGGGGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGACGCTGCCTCGTGTGGTTACCAGacac                                        <  5:23763/135‑1 (MQ=255)                                                                                                aGGGGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGACGCTGCCTCGTGTGGTTACCAGacac                                        <  2:75594/135‑1 (MQ=255)                                                                                                   tggtggCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGACGCTGCCTCGTGTGGTTACCAGACACGc                                      <  5:101396/134‑1 (MQ=255)                                                                                                     gtggCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGACGCTGCCTCGTGTGGTTACCAGACACGCAag                                   <  4:230120/135‑1 (MQ=255)                                                                                                        gCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTg                                                                                                                                  >  7:192931/1‑37 (MQ=255)                                                                                                        gCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTg                                                                                                                                  <  8:192931/37‑1 (MQ=255)                                                                                                                gAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGACGCTGCCTCGTGTGGTTACCAGACACGCAAGCAGCAGAACCg                        <  3:91083/135‑1 (MQ=255)                                                                                                                gAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGACGCTGCCTCGTGTGGTTACCAGACACGCAAGCAGCAGAACCg                        >  8:307346/1‑135 (MQ=255)                                                                                                                          cTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGACGCTGCCTCGTGTGGTTACCAGACACGCAAGCAGCAGAACCGTGCCGAAc                <  3:230667/133‑1 (MQ=255)                                                                                                                          cTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGACGCTGCCTCGTGTGGTTACCAGACACGCAAGCAGCAGAACCGTGCCGAACg               <  7:388825/134‑1 (MQ=255)                                                                                                                           tGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGACGCTGCCTCGTGTGGTTACCAGACACGCAAGCAGCAGAACCGTGCCGAACGCa             <  3:387250/135‑1 (MQ=255)                                                                                                                           tGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGACGCTGCCTCGTGTGGTTACCAGACACGCAAGCAGCAGAACCCTGCCGAACGCa             >  5:372648/1‑135 (MQ=255)                                                                                                                                 aGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGACGCTGCCTCGTGTGGTTACCAGACACGCAAGCAGCAGAACCGTGCCGAACGCACCGAGt       >  8:47318/1‑135 (MQ=255)                                                                                                                                      agcagTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACATGGGTGGGCAAGTCTGGCGGGCGCATCGACGCTGCCTCGTGTGGTTACCAGACACGCAAGCAGCAGAACCGTGCCGACCGCACCGAGTGGGaa  >  7:370368/1‑135 (MQ=255)                                                                                                                                      |                                                                                                                                       TTTCCCCATGAAATCACTGCTTTACGCCGTAACCTTATTATTTTCCTTTACCCTCCCCGCTCTGGCCAACCCGCCGGCCACCTTCACCGAAGCCAAGGTGGTGGCCAAGCAGAAGGTCTACCTGGACCAGGCCAGCAGTGCCATGGGCGACCTGTACTGCGGTTGCAAATGGACCTGGGTGGGCAAGTCTGGCGGGCGCATCGACGCTGCCTCGTGTGGTTACCAGACACGCAAGCAGCAGAACCGTGCCGAACGCACCGAGTGGGAA  >  KT2440/3812960‑3813227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑