Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,957,351 G→A 100% H100Y (CAC→TAC)  dmlR_13 ← HTH‑type transcriptional regulator DmlR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,957,3510GA99.4% 297.0 / ‑6.4 86H100Y (CAC→TAC) dmlR_13HTH‑type transcriptional regulator DmlR
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (47/38);  total (48/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCA  >  KT2440/3957218‑3957482
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aCGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACCGCGTCAGCTCGATCACCCCTTCCGGGGTAAGCATGTTGTAGCCGGCCCCCCGCGGCATCAGCAACCGCCCCATGCCGTGc                                                                                                                                    >  1:200516/1‑135 (MQ=255)
 cGGTGATCACCACGTCATGCCCTTCGGCCTGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATAACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGACGTACg                                                                                                                                   <  6:154621/135‑1 (MQ=255)
 cGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCTACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACg                                                                                                                                   <  4:17856/135‑1 (MQ=255)
 cGGTGAGCACCAGGGCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACg                                                                                                                                   <  2:156725/135‑1 (MQ=255)
        caccacGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGCCAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCCCCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTg                                                                                                                            >  5:90972/1‑135 (MQ=255)
          ccacGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCCCATCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGCCATCAGCACCACCCCCATCCCCTACGCGCAGTGc                                                                                                                           >  5:3611/1‑134 (MQ=255)
          ccacGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGc                                                                                                                           >  8:334896/1‑134 (MQ=255)
          ccacGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGc                                                                                                                           >  2:7347/1‑134 (MQ=255)
          ccacGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGc                                                                                                                           >  1:5199/1‑134 (MQ=255)
          ccacGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGc                                                                                                                           >  5:302712/1‑134 (MQ=255)
          ccacGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCa                                                                                                                          >  5:19986/1‑135 (MQ=255)
              gTCATGCCCTTCGGCCAGCGGGTCGCGGTTGCGCTGCGACAGCGTCAGCGCGATCACCACTTCCGGGTAAAGCATGATGTAGCCGGCCACCAGCGGCATAAGCAACAGCCCCATGCCCTACGGGCAGTGCAAAc                                                                                                                       >  4:293591/1‑134 (MQ=255)
               tCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACg                                                                                                                                   <  7:214160/121‑1 (MQ=255)
               tCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACg                                                                                                                                   >  8:214160/1‑121 (MQ=255)
               ccATGCCCTGCGGCCAGCGGGGCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGc                                                                                                                     <  1:347597/134‑1 (MQ=255)
                 aTGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAAc                                                                                                                       >  8:302065/1‑131 (MQ=255)
                   gCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGAt                                                                                                                 >  7:240448/1‑135 (MQ=255)
                     ccTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATgg                                                                                                               >  1:307603/1‑135 (MQ=255)
                      cTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGc                                                                                                              <  1:313240/135‑1 (MQ=255)
                      cTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGc                                                                                                              <  7:372294/135‑1 (MQ=255)
                      cTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGc                                                                                                              <  6:271044/135‑1 (MQ=255)
                      cTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGc                                                                                                              <  3:277986/135‑1 (MQ=255)
                      cTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGc                                                                                                              <  2:341396/135‑1 (MQ=255)
                      cTTCGGCCAGCGGGTCGGGGTGGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGc                                                                                                              <  3:21526/135‑1 (MQ=255)
                        tCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCcg                                                                                                            >  1:2025/1‑135 (MQ=255)
                           gCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGCTGGCCGCGa                                                                                                         >  5:103520/1‑135 (MQ=255)
                            ccAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAg                                                                                                        >  7:29375/1‑135 (MQ=255)
                            ccAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAg                                                                                                        >  3:177197/1‑135 (MQ=255)
                            ccAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAg                                                                                                        >  4:52056/1‑135 (MQ=255)
                             cAGCGGGTCGGGGGTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTGGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAgg                                                                                                       <  3:69752/135‑1 (MQ=255)
                              aGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGc                                                                                                      <  4:13169/135‑1 (MQ=255)
                                   gTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAg                                                                                                 <  7:304631/135‑1 (MQ=255)
                                                tGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTcgc                                                                                    <  6:317682/135‑1 (MQ=255)
                                                tGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTcgc                                                                                    <  7:359698/135‑1 (MQ=255)
                                                tGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTcgc                                                                                    <  7:65303/135‑1 (MQ=255)
                                                     cAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTc                                                                               >  1:5750/1‑135 (MQ=255)
                                                          tCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCa                                                                          <  6:166741/135‑1 (MQ=255)
                                                           cAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCAc                                                                         >  1:134290/1‑135 (MQ=255)
                                                           cAGCTCGATCACCACTGCCGGGTAAAGCATGTTGTAGCCGGCCCCCAGCGGCATCAGCAACAGCCCCATGCCGGACGGGCAGTGCAAACGCAGATGGCCGCGCGGCGGAAGGTGCGCCCCTCGCTCCTCGCCCAc                                                                         >  2:241467/1‑135 (MQ=255)
                                                               tCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGct                                                                     <  7:19592/135‑1 (MQ=255)
                                                               tCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGct                                                                     >  5:153882/1‑135 (MQ=255)
                                                                  aTCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGcttct                                                                  <  3:52056/135‑1 (MQ=255)
                                                                     accacTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTtcgtcg                                                               >  7:280283/1‑135 (MQ=255)
                                                                     accacTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCg                                                               >  1:31786/1‑135 (MQ=255)
                                                                     accacTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTGCTTCg                                                               >  1:202730/1‑135 (MQ=255)
                                                                      ccacTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCgg                                                              <  2:5750/135‑1 (MQ=255)
                                                                      ccacTTCCGGGTAAAGCATGTTGTAGACGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCgg                                                              >  6:116584/1‑135 (MQ=255)
                                                                       gacTTCCGTGTAAAGCATGTTGTAGCCGGCCAGCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGGAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGt                                                             <  8:280283/134‑1 (MQ=255)
                                                                       cacTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGAGACTCGCCCACGGCTTCTTCGGt                                                             <  3:93192/135‑1 (MQ=255)
                                                                                gTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCAt                                                    <  1:167286/135‑1 (MQ=255)
                                                                                gTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGAGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCAt                                                    <  4:23460/135‑1 (MQ=255)
                                                                                    aGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGACGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAACGTGAGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCACCATGATc                                                >  3:341867/1‑135 (MQ=255)
                                                                                       aTGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGc                                             >  1:257552/1‑135 (MQ=255)
                                                                                       aTGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCATCTTCGGTCAGCAGCATGATCTGc                                             >  5:11511/1‑135 (MQ=255)
                                                                                           tgtAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCcgc                                          <  4:37102/134‑1 (MQ=255)
                                                                                           tgtAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCcgc                                          <  7:4757/134‑1 (MQ=255)
                                                                                            gtAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGCGGCGTAAGGTGCGCCCCTCGCGCCTCGCCGACGGCTTCTTCGGTCAGCAGCATGATCTGCCgcgc                                        >  1:87998/1‑135 (MQ=255)
                                                                                            gtAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCgcgc                                        >  8:205175/1‑135 (MQ=255)
                                                                                                 cGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCt                                   >  4:110926/1‑135 (MQ=255)
                                                                                                  ggCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTc                                  >  2:175436/1‑135 (MQ=255)
                                                                                                  ggCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTc                                  >  1:217478/1‑135 (MQ=255)
                                                                                                   gCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCt                                   >  6:330337/1‑133 (MQ=255)
                                                                                                     caccaGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAg                               <  6:11511/135‑1 (MQ=255)
                                                                                                      accaGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGc                                                                                                              <  1:53953/55‑1 (MQ=255)
                                                                                                      accaGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGc                                                                                                              >  2:53953/1‑55 (MQ=255)
                                                                                                             gCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCCCGGCTTCTTCGGTCAGCCGCATGATCTGCCGCGCACGCTCCAGAAACCGc                        >  5:165429/1‑134 (MQ=255)
                                                                                                             gCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGc                        >  4:248626/1‑134 (MQ=255)
                                                                                                             gCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCt                       <  6:328172/135‑1 (MQ=255)
                                                                                                                   gCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCg                 <  1:175436/135‑1 (MQ=255)
                                                                                                                           cccATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTc         >  8:98327/1‑135 (MQ=255)
                                                                                                                             cATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGCGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCCCGCTCCAGAAACCGCTCACCCGCCTCGCTCAt       >  5:103411/1‑135 (MQ=255)
                                                                                                                             cATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCg                 >  2:374731/1‑125 (MQ=255)
                                                                                                                             cATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCg                 <  1:374731/125‑1 (MQ=255)
                                                                                                                             cATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCAt       <  2:217478/135‑1 (MQ=255)
                                                                                                                             cATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCAt       >  2:83020/1‑135 (MQ=255)
                                                                                                                              aTGCCGTACGGGCTGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGAACGCTCCAGAAACCGCTCACCCGCCTCGCTCATg      <  3:95614/135‑1 (MQ=255)
                                                                                                                              aTGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATg      <  7:4952/135‑1 (MQ=255)
                                                                                                                               tGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATgc     >  3:54409/1‑135 (MQ=255)
                                                                                                                                gCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATgcg    <  4:312532/135‑1 (MQ=255)
                                                                                                                                 ccGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCcgcg                                         <  2:258245/97‑1 (MQ=255)
                                                                                                                                 ccGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCcgcg                                         >  1:258245/1‑97 (MQ=255)
                                                                                                                                 ccGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCCTgc     >  8:190189/1‑133 (MQ=255)
                                                                                                                                  cGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCCCGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCa  >  6:127269/1‑135 (MQ=255)
                                                                                                                                  cGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCCCGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCa  >  7:177383/1‑135 (MQ=255)
                                                                                                                                  cGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCAt       <  7:250106/130‑1 (MQ=255)
                                                                                                                                  cGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCAt       >  8:250106/1‑130 (MQ=255)
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ACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCA  >  KT2440/3957218‑3957482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑