Predicted mutation
evidence seq id position mutation annotation gene description
RA KT2440 1,806,731 G→T K10N (AAG→AAT)  yeiG → S‑formylglutathione hydrolase YeiG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,806,7310GT98.0% 175.2 / ‑5.3 49K10N (AAG→AAT) yeiGS‑formylglutathione hydrolase YeiG
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (27/21);  total (27/22)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

CATCACCCACACGATGGGGCTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGC  >  KT2440/1806603‑1806860
                                                                                                                                |                                                                                                                                 
catcaCCCACACGATGGGGCTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTc                                                                                                                             >  1:29554/1‑135 (MQ=255)
  tcaCCCACACGATGGGGCTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTcgg                                                                                                                           <  3:129065/135‑1 (MQ=255)
     cccACACGATGGGGCTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTcggcgg                                                                                                                        >  4:206773/1‑135 (MQ=255)
         cacGATGGGGCTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTgg                                                                                                                    <  1:89480/135‑1 (MQ=255)
          acGATGGGGCTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGc                                                                                                                   >  7:113179/1‑135 (MQ=255)
          acGAAGGGGCTGGAAGACATCAACAAGGCCTTCGCCCTGATCCATGAAGGCAAGAGCATCCGTAGCGTCATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTCGc                                                                                                                   >  8:212822/1‑135 (MQ=255)
           cGATGGGGCTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGc                                                                                                                   >  4:170868/1‑134 (MQ=255)
                   cTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTTGATAACATCTCCTGCCAGAATAGCTTcggc                                                                                                                          <  4:195801/119‑1 (MQ=255)
                   cTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTTGATAACATCTCCTGCCAGAATAGCTTcggc                                                                                                                          >  3:195801/1‑119 (MQ=255)
                   cTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGtt                                                                                                          >  6:3076/1‑135 (MQ=255)
                    tGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTa                                                                                                         >  7:30610/1‑135 (MQ=255)
                       aaGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCg                                                                                                      <  8:30610/135‑1 (MQ=255)
                               aacaaGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCAtt                                                                                              <  6:143136/135‑1 (MQ=255)
                                    ggCCTTCGACATGATGCATGAATGCAAGAGCCGCCGTAGCGTGCTCGACTTCTGATGTCTGTCCTGAGCCTGGATAAACTCTCCTGCCAGAAGAGCGTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCacg                                                                                         <  5:95238/135‑3 (MQ=255)
                                           gACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCt                                                                                    <  3:115772/133‑1 (MQ=255)
                                           gACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCt                                                                                    <  5:44098/133‑1 (MQ=255)
                                           gACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCt                                                                                    <  5:3076/133‑1 (MQ=255)
                                                   gCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGat                                                                          >  3:137887/1‑135 (MQ=255)
                                                         aGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGgt                                                                     <  3:170868/134‑1 (MQ=255)
                                                         aGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGgt                                                                     <  2:157882/134‑1 (MQ=255)
                                                         aGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATAAGgtg                                                                    >  8:173004/1‑135 (MQ=255)
                                                         aGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCATCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGgt                                                                     <  8:187969/134‑1 (MQ=255)
                                                          ggCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGgtgt                                                                   <  7:35363/135‑1 (MQ=255)
                                                                  cATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCtgt                                                            >  5:75339/1‑134 (MQ=255)
                                                                                  cACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTCCAGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTCTTCGCTGTGTACCTGCCGCCGCAgg                                           >  1:200990/1‑135 (MQ=255)
                                                                                  cACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAgg                                           >  1:190516/1‑135 (MQ=255)
                                                                                   aCTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGc                                          >  4:213828/1‑135 (MQ=255)
                                                                                             tCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCg                                                                                                      >  8:111834/1‑65 (MQ=255)
                                                                                             tCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCg                                                                                                      <  7:111834/65‑1 (MQ=255)
                                                                                                  cATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGgt                                                                     <  5:113856/93‑1 (MQ=255)
                                                                                                  cATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGgt                                                                     >  6:113856/1‑93 (MQ=255)
                                                                                                  cATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGaaaa                           >  8:92259/1‑135 (MQ=255)
                                                                                                  cATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGaaaa                           >  7:126888/1‑135 (MQ=255)
                                                                                                  cATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGaaaa                           >  8:147619/1‑135 (MQ=255)
                                                                                                    tGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGc                         <  7:92259/135‑1 (MQ=255)
                                                                                                    tGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGc                         >  1:86788/1‑135 (MQ=255)
                                                                                                         cTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCg                    >  3:203449/1‑135 (MQ=255)
                                                                                                         cTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCg                    >  3:176315/1‑135 (MQ=255)
                                                                                                         cTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCg                    <  3:107531/135‑1 (MQ=255)
                                                                                                         cTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCg                    <  2:29554/135‑1 (MQ=255)
                                                                                                         cTGGATAACATCTCCTGCAAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCg                    >  7:183119/1‑135 (MQ=255)
                                                                                                              tAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCAtt                                                                                              <  7:23792/56‑1 (MQ=255)
                                                                                                              tAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCAtt                                                                                              >  8:23792/1‑56 (MQ=255)
                                                                                                              tAACATCTCCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCt               >  6:11390/1‑135 (MQ=255)
                                                                                                                    ctcCTGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTg         >  1:143458/1‑135 (MQ=255)
                                                                                                                        tGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGc       <  1:116108/133‑1 (MQ=255)
                                                                                                                        tGCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGc       <  8:183119/133‑1 (MQ=255)
                                                                                                                         gCCAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCa    >  7:64199/1‑135 (MQ=255)
                                                                                                                           cAGAATAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGc  <  6:75339/135‑1 (MQ=255)
                                                                                                                            agaataGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCg                    <  3:193883/116‑1 (MQ=255)
                                                                                                                            agaataGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCg                    >  4:193883/1‑116 (MQ=255)
                                                                                                                                |                                                                                                                                 
CATCACCCACACGATGGGGCTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGC  >  KT2440/1806603‑1806860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑