Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	KT2440	3,957,351	G→A	H100Y (CAC→TAC)	dmlR_13 ←	HTH‑type transcriptional regulator DmlR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	3,957,351	0	G	A	100.0%	98.9 / NA	28	H100Y (CAC→TAC)	dmlR_13	HTH‑type transcriptional regulator DmlR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/9);  total (19/9)

GATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGC  >  KT2440/3957223‑3957481                                                                                                                                 |                                                                                                                                   gATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCa                                                                                                                              >  1:5647/1‑135 (MQ=255) gATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCa                                                                                                                              >  1:5650/1‑135 (MQ=255)     accacGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCCCCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTg                                                                                                                           >  6:143566/1‑134 (MQ=255)      ccacGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGCTCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCACCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGc                                                                                                                          >  5:72349/1‑134 (MQ=255)        acGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCaaa                                                                                                                       >  1:40688/1‑135 (MQ=255)                  cTTCGGCCAGCGTGTCGGGGTTGCGCTGCGACCGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGc                                                                                                             <  7:97581/135‑1 (MQ=255)                  cTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGc                                                                                                             >  4:158157/1‑135 (MQ=255)                       gCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCCCCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGa                                                                                                        >  3:203255/1‑135 (MQ=255)                         cAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAgg                                                                                                      <  3:10986/135‑1 (MQ=255)                               gTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAgg                                                                                                      >  6:213480/1‑129 (MQ=255)                               gTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAgg                                                                                                      <  5:213480/129‑1 (MQ=255)                                            tGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTcgc                                                                                   >  4:196367/1‑135 (MQ=255)                                                       cAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCAc                                                                        <  1:15425/135‑1 (MQ=255)                                                           tCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGct                                                                    >  8:18601/1‑135 (MQ=255)                                                              aTCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGcttc                                                                  <  8:54660/134‑1 (MQ=255)                                                                  ccacTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCgg                                                             <  3:158157/135‑1 (MQ=255)                                                                              aaaGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGa                                                 >  6:201518/1‑135 (MQ=255)                                                                                aGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATc                                               >  3:125817/1‑135 (MQ=255)                                                                                        gtAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCgcgc                                       >  1:148676/1‑135 (MQ=255)                                                                                              ggCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCt                                  >  1:20706/1‑134 (MQ=255)                                                                                               gCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCt                                  >  7:155328/1‑133 (MQ=255)                                                                                                   ccaGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCCCGGCGGCTGCGGTCAGCCGCCTGATCTGCCGCGCGCGCTCCAGaa                            >  3:128276/1‑135 (MQ=255)                                                                                                      gCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAAcc                         >  4:19191/1‑135 (MQ=255)                                                                                                               gCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCg                <  5:43294/135‑1 (MQ=255)                                                                                                                          ttGCGGTACGGGCAGGGCAAACGCAGAGGGCGGCTAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCAGGGCTTCTTCGGGCAGCAGCATGATGTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCGCGCTCATg     <  4:203255/134‑1 (MQ=255)                                                                                                                          aTGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATg     <  2:40688/135‑1 (MQ=255)                                                                                                                             ccGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATgc    >  2:21396/1‑133 (MQ=255)                                                                                                                             ccGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCGTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATgcgc  >  6:119479/1‑135 (MQ=255)                                                                                                                                 |                                                                                                                                   GATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGC  >  KT2440/3957223‑3957481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑