Predicted mutation
evidence seq id position mutation annotation gene description
RA KT2440 3,061,793 G→A S592N (AGC→AAC)  qedA_2 → Quinoprotein alcohol dehydrogenase (cytochromec)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,061,7930GA95.9% 149.8 / ‑5.8 50S592N (AGC→AAC) qedA_2Quinoprotein alcohol dehydrogenase (cytochromec)
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (34/13);  minor base C (0/2);  total (35/15)
Fisher's exact test for biased strand distribution p-value = 8.93e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAGCGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGC  >  KT2440/3061659‑3061926
                                                                                                                                      |                                                                                                                                     
ggTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAgcaaacggcaaacg                                                                                                                                     <  1:87180/136‑1 (MQ=255)
ggTTTCGGTAGGCTCCGGCTGGGGCGGCGCGGTGCAGCTGGGGGGCGGCGAAGTGGCCAAACGGGTCAATGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAgcaaacggcaaacg                                                                                                                                     <  3:27834/136‑1 (MQ=255)
cGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGCCTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAgcaaacggcaaacg                                                                                                                                     <  3:391564/137‑1 (MQ=255)
cGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAgcaaacggcaaacg                                                                                                                                     <  6:395871/137‑1 (MQ=255)
cGTTTCGGTAGGCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAgcaaacggcaaacg                                                                                                                                     <  7:120861/137‑1 (MQ=255)
     cGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCaac                                                                                                                                >  3:253619/1‑136 (MQ=255)
        tAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCACACGGTTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCATCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTAAAGCCa                                                                                                                             >  7:336779/1‑137 (MQ=255)
           tctcCGGCTGGGTCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATaa                                                                                                                          <  7:52245/137‑1 (MQ=255)
           tctcCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATaa                                                                                                                          <  8:74552/137‑1 (MQ=255)
             tcCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACg                                                                                                                        >  8:10651/1‑137 (MQ=255)
              ccGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACgc                                                                                                                       >  1:88493/1‑137 (MQ=255)
                ggCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACgcct                                                                                                                     >  4:87320/1‑137 (MQ=255)
                     gggCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTa                                                                                                                >  2:446876/1‑137 (MQ=255)
                     gggCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTa                                                                                                                >  2:132157/1‑137 (MQ=255)
                     gggCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTa                                                                                                                <  6:433712/137‑1 (MQ=255)
                          gcgcGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGACCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATCACGCCTGCCTACTGcc                                                                                                           >  8:389546/1‑137 (MQ=255)
                              ggTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCtt                                                                                                       >  3:91516/1‑137 (MQ=255)
                                       gtgGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCt                                                                                              <  7:103407/137‑1 (MQ=255)
                                       gtgGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCt                                                                                              <  4:381547/137‑1 (MQ=255)
                                           ggcggcGAAGTGGCCAAACGGGGCAAGGACTTAAACCAGGGAGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATc                                                                                          <  8:212339/137‑1 (MQ=255)
                                             aggcgAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCAATAACGCCTGCCTACTTCCTCTTGCTGCCGCTGATCgg                                                                                        >  6:444916/2‑137 (MQ=255)
                                                cgAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACTTCAAGCCATAACGCCTGCCTACTGCCGCTTGCGGCCGCTGATCGGTgg                                                                                     >  5:5404/1‑137 (MQ=255)
                                                 gatgTGGCCAAACGGGTCAAGCACTTCACCCAGGGCGGCATGCTCTGGACCTTCACGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCACCCCATAACGCCGGCCTCCTGCCGCTTGCTGCCGCTGATCGGGGGc                                                                                    <  6:309033/134‑1 (MQ=255)
                                                  aaGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCa                                                                                   >  7:148289/1‑137 (MQ=255)
                                                   aGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCaa                                                                                  >  6:419028/1‑137 (MQ=255)
                                                     tGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAgg                                                                                >  1:44646/1‑136 (MQ=255)
                                                       gCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCgg                                                                              <  5:419028/137‑1 (MQ=255)
                                                         cAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGcc                                                                            >  5:134475/1‑137 (MQ=255)
                                                         aaaaCGGTGCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAACCGGCAACCGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATGGGTGGCAAGCGGcc                                                                            <  6:317546/136‑1 (MQ=255)
                                                          aaaCGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGccc                                                                           >  4:159073/1‑137 (MQ=255)
                                                               ggTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGGCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCctacta                                                                      >  4:143825/1‑137 (MQ=255)
                                                                     ttaCTTAAACCTGGGGGGCAGGCTCTTGACATTAATTTTGCCCATGCTGTTGACCAAAACGCAAAACTTAAAGCCATACCGCCGGAATACTGCCGTTTGCTGCCGCTGATCGGTGGCATGCGGCCCTACTACCAAAt                                                                <  4:41822/135‑1 (MQ=255)
                                                                             aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGagag                                                        >  6:393330/1‑137 (MQ=255)
                                                                             aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGAACGGTGGCAAGCGGCCCTACTACAAAATGACgagat                                                        >  3:65682/1‑136 (MQ=255)
                                                                              ccAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAGCGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGc                                                       >  6:337623/1‑137 (MQ=255)
                                                                                 gggCGGCATGCTCTGGACCTTCAAGTGGCCCAAGAAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTAATTCCGCTTGCTGCCGCTGAGCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGcccc                                                    <  5:426194/137‑1 (MQ=255)
                                                                                    cggcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCt                                                 >  6:218577/1‑137 (MQ=255)
                                                                                    cggcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCGACTACCAAATGACGAGAGCCCCGcg                                                 >  4:258945/1‑136 (MQ=255)
                                                                                     ggcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTg                                                >  6:182260/1‑137 (MQ=255)
                                                                                     ggcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTg                                                >  2:45549/1‑137 (MQ=255)
                                                                                      gcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGt                                                                                                                                    >  5:460258/1‑52 (MQ=255)
                                                                                      gcATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGt                                                                                                                                    <  6:460258/52‑1 (MQ=255)
                                                                                               ggACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGc                                      >  8:374097/1‑137 (MQ=255)
                                                                                               ggACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGc                                      >  1:154766/1‑137 (MQ=255)
                                                                                                        aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGcccc                             >  3:26646/1‑136 (MQ=255)
                                                                                                        aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCg                             >  7:441923/1‑137 (MQ=255)
                                                                                                        aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCg                             >  3:176499/1‑137 (MQ=255)
                                                                                                        aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCg                             >  8:213721/1‑137 (MQ=255)
                                                                                                        aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGAGGCATTCGCCCg                             >  6:200959/1‑137 (MQ=255)
                                                                                                                  ccAGTTGCCCCAACCGCAAAACGCCAACCCATACCCCCGCCCAACTGCCGCTTGCTGCCGCTGAGCGGTGCCACGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCgggg                   <  3:87320/136‑1 (MQ=255)
                                                                                                                     ggcgcagcaAAGGGCAACCGGCACCCCAAACCGCCTCCCTACCGCCGCTTGCTGCCGCTGAGCGGTGCCATGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGctt                <  2:120992/134‑1 (MQ=255)
                                                                                                                          agcaAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTaa           >  3:387248/1‑136 (MQ=255)
                                                                                                                           ccaAACGGCAAACGTCAAGCCATACCGCCTGCCTACTGCCGCTGGCTGCCGATGATCGGGGGCAAGCGGCCCTACTCCCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTAcc          <  6:207516/136‑1 (MQ=255)
                                                                                                                                 ggCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGt    >  3:431095/1‑137 (MQ=255)
                                                                                                                                   cAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGc  >  7:233496/1‑137 (MQ=255)
                                                                                                                                      |                                                                                                                                     
CGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAGCGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGC  >  KT2440/3061659‑3061926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑