Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	KT2440	3,155,891	G→T	D68Y (GAT→TAT)	acpP_2 →	Acyl carrier protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	3,155,891	0	G	T	95.5%	224.2 / 1.7	67	D68Y (GAT→TAT)	acpP_2	Acyl carrier protein
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (36/27);  total (38/29)
Fisher's exact test for biased strand distribution p-value = 5.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

TAGGGGCGACGTGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGGATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAGGACGGCGTGGCCTTGCTGGAGCAGAGCCTCAGCGCTTATGCGCCGGGCCAGTTGCAC  >  KT2440/3155764‑3156010                                                                                                                                |                                                                                                                        tAGGGGCGACGTGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACTGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCgg                                                                                                                <  5:220567/137‑1 (MQ=255)       cGACGTGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCgg                                                                                                          >  1:274003/1‑137 (MQ=255)            tGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCTACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAAACAGCGTGCTGTATCACGCGGTAGCGGTGtta                                                                                                     >  2:235076/1‑136 (MQ=255)            tGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCTTCAGTACGGTTGAACCTTGCCGAAAACCAGCGTGCTGTATAACGCGGTAGAGGTGtta                                                                                                     >  5:379889/1‑136 (MQ=255)            tGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCgg                                                                                                                <  1:179300/126‑1 (MQ=255)            tGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCgg                                                                                                                >  2:179300/1‑126 (MQ=255)            tGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTc                                                                                                     >  7:369690/1‑137 (MQ=255)                    cacaGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGc                                                                                             >  6:411513/1‑137 (MQ=255)                     acaGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGcc                                                                                            >  5:123536/1‑137 (MQ=255)                          agTATTGTTGACGACCTCATCGCAGACTCGCTGGACATCGTCGACATGAGCTTACAGCTGGGCCGTCAGTACGGTTTCACCTTGCCGAAAACCAGCGAGCTGAATCACGCCGTAGAGGTGTTCGTCGAAGCCAccag                                                                                       >  7:52400/1‑135 (MQ=255)                          agTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGCCCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCg                                                                                       >  5:366953/1‑137 (MQ=255)                          agTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCg                                                                                       >  5:344131/1‑137 (MQ=255)                            tATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCCCCCGGt                                                                                     >  1:377016/1‑137 (MQ=255)                            tATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGt                                                                                     >  6:336565/1‑137 (MQ=255)                               tgttgACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTg                                                                                  >  8:1207/1‑137 (MQ=255)                               tgttgACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTg                                                                                  >  1:48595/1‑137 (MQ=255)                                 ttgACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTc                                                                                <  8:352149/137‑1 (MQ=255)                                 ttgACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTc                                                                                >  5:80896/1‑137 (MQ=255)                                 ttgACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTACCGGTGTTCGGCGACGCCACCCGGTTTGTc                                                                                >  5:73880/1‑137 (MQ=255)                                    acgacCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGaa                                                                             >  3:332665/1‑137 (MQ=255)                                    acgacCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGaa                                                                             >  4:111397/1‑137 (MQ=255)                                    acgacCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGGATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGaa                                                                             >  1:268222/1‑137 (MQ=255)                                      gacCTCAGCGCCGACTCGCTGGACATCGTCGCCCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGGTTGTCGaaaa                                                                           >  7:128116/1‑137 (MQ=255)                                      gacCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGaaaa                                                                           >  3:54154/1‑137 (MQ=255)                                       acCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAAt                                                                          >  6:419691/1‑137 (MQ=255)                                         cTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATgg                                                                        >  8:364949/1‑137 (MQ=255)                                         cTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATgg                                                                        >  8:41151/1‑137 (MQ=255)                                           cAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCTGTTTGTCGAAAATGGcc                                                                      <  2:382084/137‑1 (MQ=255)                                           cAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGcc                                                                      <  6:123536/137‑1 (MQ=255)                                           cAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGcc                                                                      <  5:194988/137‑1 (MQ=255)                                           cAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGcc                                                                      >  4:322822/1‑137 (MQ=255)                                           cAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGcc                                                                      <  2:145569/137‑1 (MQ=255)                                           cAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGcc                                                                      <  3:363267/137‑1 (MQ=255)                                           cAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGcc                                                                      >  3:290339/1‑137 (MQ=255)                                           cAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGcc                                                                      <  2:48595/137‑1 (MQ=255)                                                  gACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCAc                                                               <  4:256107/137‑1 (MQ=255)                                                      cGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAg                                                           >  8:229315/1‑137 (MQ=255)                                                           gACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGGATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAGGACgg                                                      <  2:268222/137‑1 (MQ=255)                                                              aTCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCTGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAGGACGGCGt                                                   <  7:201454/137‑1 (MQ=255)                                                              aTCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCCCCCGGTTTGTCGAAAATGGCCGAATCACGCAGGACGGCgg                                                   >  2:190386/1‑136 (MQ=255)                                                              aTCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAGGACGGCGt                                                   <  6:248171/137‑1 (MQ=255)                                                              aTCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAGGACGGCGt                                                   <  2:373453/137‑1 (MQ=255)                                                                cgtcgACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAGGACGGCGTgg                                                 <  4:290339/137‑1 (MQ=255)                                                                   cgACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAGGACGGCGTGGCCt                                              >  8:376428/1‑137 (MQ=255)                                                                   cgACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAGGACGGCGTGGCCt                                              <  4:336836/137‑1 (MQ=255)                                                                   cgACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAGGACGGCGTGGCCt                                              <  8:25016/137‑1 (MQ=255)                                                                   cgACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAGGACGGCGTGGCCt                                              <  4:332665/137‑1 (MQ=255)                                                                          aGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAGGACGGCGTGGCCTTGCTGGa                                       <  7:413330/137‑1 (MQ=255)                                                                           gCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAGGACGGCGTGGCCTTGCTGGAg                                      <  2:274003/137‑1 (MQ=255)                                                                           gCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAGGACGGCGTGGCCTTGCTGGAg                                      <  5:231522/137‑1 (MQ=255)                                                                             ttCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAGGACGGCGTGGCCTTGCTGGAGcc                                    >  2:12832/1‑136 (MQ=255)                                                                                cAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAGGACGGCGTGGCCTTGCTGGAGCagag                                 <  5:408393/137‑1 (MQ=255)                                                                                cAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGGATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAGGACGGCGTGGCCTTGCTGGAGCagag                                 <  7:227366/137‑1 (MQ=255)                                                                                   cTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAGGACGGCGTGGCCTTGCTGGAGCAGAGCCt                              <  2:37890/137‑1 (MQ=255)                                                                                         cGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAGGACGGCGTGGCCTTGCTGGAGCAGAGCCTCAgcgc                        >  1:268603/1‑137 (MQ=255)                                                                                              gTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAGGACGGCGTGGCCTTGCTGGAGCAGAGCCTCAGCGCTTATg                   <  1:188123/137‑1 (MQ=255)                                                                                              gTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAGGACGGCGTGGCCTTGCTGGAGCAGAGCCTCAGCGCTTATg                   <  2:1385/137‑1 (MQ=255)                                                                                              gTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAGGACGGCGTGGCCTTGCTGGAGCAGAGCCTCAGCGCTTATg                   <  2:8914/137‑1 (MQ=255)                                                                                              gTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAGAACGGCGTGGCCTTGCTGGAGCAGAGCCTCAGCGCTTATg                   <  1:264906/137‑1 (MQ=255)                                                                                                     tGCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAGGACGGCGTGGCCTTGCTGGAGCAGAGCCTCAGCGCTTATGCGCCggg            >  2:280820/1‑137 (MQ=255)                                                                                                      gCACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAGGACGGCGTGGCCTTGCTGGAGCAGAGCCTCAGCGCTTATGCGCCGGGc           >  8:120544/1‑137 (MQ=255)                                                                                                       cACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATTACGCAGGACGGCGTGGCCtt                                             >  1:279429/1‑102 (MQ=255)                                                                                                       cACCTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATTACGCAGGACGGCGTGGCCtt                                             <  2:279429/102‑1 (MQ=255)                                                                                                         ccTTGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAGGACGGCGTGGCCTTGCTGGAGCAGAGCCTCAGCGCTTATGCGCCGGGCCAg        >  3:77175/1‑137 (MQ=255)                                                                                                            tGCCGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAGGACGGCGTGGCCTTGCTGGAGCAGAGCCTCAGCGCTTATGCGCCGGGCCAGTTg     >  6:80452/1‑137 (MQ=255)                                                                                                               cGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAGGACGGCGTGGCCTTGCTGGAGCAGCGCCTCAGCGCTTATGCGCCGGGCCAGTTGCAc  >  8:386662/1‑137 (MQ=255)                                                                                                               cGAAAACCAGCGTGCTGTATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAGGACGGCGTGGCCTTGCTGGAGCAGAGCCTCAGCGCTTATGCGCCGGGCCAGTTGCAc  >  1:65856/1‑137 (MQ=255)                                                                                                                                |                                                                                                                        TAGGGGCGACGTGACAGAGCACAGCAGTATTGTTGACGACCTCAGCGCCGACTCGCTGGACATCGTCGACCTGAGCTTCCAGCTGGGCCGTCAGTACGGTTGCACCTTGCCGAAAACCAGCGTGCTGGATCACGCGGTAGCGGTGTTCGGCGACGCCACCCGGTTTGTCGAAAATGGCCGAATCACGCAGGACGGCGTGGCCTTGCTGGAGCAGAGCCTCAGCGCTTATGCGCCGGGCCAGTTGCAC  >  KT2440/3155764‑3156010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑