Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	KT2440	3,957,351	G→A	H100Y (CAC→TAC)	dmlR_13 ←	HTH‑type transcriptional regulator DmlR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	3,957,351	0	G	A	100.0%	211.9 / NA	59	H100Y (CAC→TAC)	dmlR_13	HTH‑type transcriptional regulator DmlR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/31);  total (28/31)

CACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAG  >  KT2440/3957217‑3957483                                                                                                                                       |                                                                                                                                     cACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACg                                                                                                                                    <  7:43282/137‑1 (MQ=255) cACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACg                                                                                                                                    <  1:59798/137‑1 (MQ=255) cACGGGTATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACg                                                                                                                                    <  3:129117/137‑1 (MQ=255)  aCGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACgg                                                                                                                                   >  2:420164/1‑137 (MQ=255)  aCGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACgg                                                                                                                                   >  4:390836/1‑137 (MQ=255)            ccacGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCACCAGCCCCATCCCGTACGGGCAGTGCaaa                                                                                                                         >  8:87689/1‑137 (MQ=255)            ccacGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCaaa                                                                                                                         >  2:131285/1‑137 (MQ=255)                gTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTACGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCa                                                                                                                     >  1:210456/1‑137 (MQ=255)                      cccTTCGGCCAGGGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTTGCCGGCCACCAGCGTCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACTCAGATGGc                                                                                                               <  1:202699/137‑1 (MQ=255)                      cccTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGc                                                                                                               <  8:65298/137‑1 (MQ=255)                      cccTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGc                                                                                                               <  7:318807/137‑1 (MQ=255)                      cccTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGc                                                                                                               <  1:207001/137‑1 (MQ=255)                       ccTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGcc                                                                                                              >  7:346214/1‑137 (MQ=255)                             gCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAgg                                                                                                        <  4:4062/137‑1 (MQ=255)                             gCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACATACGGGTAAAGCATGTTGTAGCCGGCCCCCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGCgg                                                                                                        >  1:347039/1‑137 (MQ=255)                             gCCAGCGGGTCGGGGGTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAgg                                                                                                        <  7:129465/137‑1 (MQ=255)                                     gTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGt                                                                                                <  5:275065/137‑1 (MQ=255)                                     gTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGt                                                                                                >  8:359893/1‑137 (MQ=255)                                     gTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGt                                                                                                <  2:202355/137‑1 (MQ=255)                                     gTCGGGGTTGCGCTGCGACAGCGTAAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGt                                                                                                <  6:217217/137‑1 (MQ=255)                                                 cTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTcgcg                                                                                    >  3:55576/1‑137 (MQ=255)                                                     gACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTc                                                                                <  2:373636/137‑1 (MQ=255)                                                       cAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGc                                                                              >  6:80171/1‑137 (MQ=255)                                                               gCTCGATCACCACTTCCGGGTCAAGCATGTTGTAGCCGGCCAACAGCGGCATCAGCAACAGCCCCATGCCGCACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGct                                                                      <  7:309251/137‑1 (MQ=255)                                                               gCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGct                                                                      <  4:348821/137‑1 (MQ=255)                                                                  cGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGcttct                                                                   <  4:55576/137‑1 (MQ=255)                                                                    aTCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCttcttc                                                                 <  5:80171/137‑1 (MQ=255)                                                                      caccacTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCgg                                                               <  4:393769/137‑1 (MQ=255)                                                                       cccacTTCCGGGTACAGAATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGt                                                              <  2:151026/136‑1 (MQ=255)                                                                       accacTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGt                                                              >  2:128462/1‑137 (MQ=255)                                                                       accacTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGCTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGt                                                              >  4:361204/1‑137 (MQ=255)                                                                        ccacTTCCGGGTAAAGCATGTTGTAGACGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCCCGCCTCGCCCACGGCTTCGTCGGTc                                                             >  3:430110/1‑137 (MQ=255)                                                                           cTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTcagc                                                          <  8:53682/137‑1 (MQ=255)                                                                                      aGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTcgcg                                                                                    >  2:396191/1‑100 (MQ=255)                                                                                      aGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTg                                               >  7:365957/1‑137 (MQ=255)                                                                                      aGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGAAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTcgcg                                                                                    <  1:396191/100‑1 (MQ=255)                                                                                         aTGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCcg                                            >  1:17894/1‑137 (MQ=255)                                                                                         aTGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCcg                                            >  4:226399/1‑137 (MQ=255)                                                                                          tgttgtAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCcgc                                           <  3:390836/137‑1 (MQ=255)                                                                                           tttgtAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCAACGGCTTCTTCGGTCAGCAGCATGATCTGCcgcg                                          <  8:5060/136‑1 (MQ=255)                                                                                           gttgtAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCcgcg                                          >  6:305667/1‑137 (MQ=255)                                                                                           gttgtAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCcgcg                                          <  4:37160/137‑1 (MQ=255)                                                                                           gttgtAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCcgcg                                          <  7:345672/137‑1 (MQ=255)                                                                                           gttgtAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCcgcg                                          <  7:359893/137‑1 (MQ=255)                                                                                               tAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACg                                      >  3:341446/1‑137 (MQ=255)                                                                                                    ggCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCa                                 >  7:159521/1‑137 (MQ=255)                                                                                                    ggCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCa                                 >  2:327445/1‑137 (MQ=255)                                                                                                       caccaGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGaa                              >  8:376461/1‑137 (MQ=255)                                                                                                        accaGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGaaa                             >  7:191277/1‑137 (MQ=255)                                                                                                         ccaGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAAc                            >  3:126111/1‑137 (MQ=255)                                                                                                                  tCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCAccc                   >  5:357222/1‑137 (MQ=255)                                                                                                                    aGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGc                 >  7:328025/1‑137 (MQ=255)                                                                                                                              ccATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATg       >  4:290965/1‑137 (MQ=255)                                                                                                                               cATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCa                                        <  7:93644/103‑1 (MQ=255)                                                                                                                               cATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCa                                        >  8:93644/1‑103 (MQ=255)                                                                                                                               cATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATgc      <  3:294847/137‑1 (MQ=255)                                                                                                                                   ccGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAg  <  3:146919/137‑1 (MQ=255)                                                                                                                                   ccGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAg  <  7:87689/137‑1 (MQ=255)                                                                                                                                   ccGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAg  <  1:164637/137‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     CACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCAG  >  KT2440/3957217‑3957483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑