breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 136 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913580,4310TA76.9% 9.9 / 12.4 26I125I (ATT→ATA) ‡nohBbacteriophage DNA packaging protein
*NC_0009131,634,0190TC65.8% 37.8 / 37.4 41I125V (ATT→GTT) ‡nohANohA
*NC_000913580,4290AG64.5% 28.4 / 27.5 31I125V (ATT→GTT) ‡nohBbacteriophage DNA packaging protein
*NC_000913580,4250TA62.2% 44.1 / 48.2 45G123G (GGT→GGAnohBbacteriophage DNA packaging protein
*NC_0009133,192,8730TC59.7% 298.4 / inf 457intergenic (+332/+88)b3051/b3052putative membrane protein/putative kinase
*NC_0009133,192,8910CT58.7% 351.2 / inf 443intergenic (+350/+70)b3051/b3052putative membrane protein/putative kinase
*NC_0009133,192,9070AT56.0% 234.7 / inf 440intergenic (+366/+54)b3051/b3052putative membrane protein/putative kinase
*NC_0009133,192,9050CA55.6% 26.0 / inf 438intergenic (+364/+56)b3051/b3052putative membrane protein/putative kinase
*NC_0009131,634,0230AT55.1% 63.5 / 72.7 49G123G (GGT→GGAnohANohA
*NC_000913580,4220AC54.5% 73.9 / 76.5 55A122A (GCA→GCCnohBbacteriophage DNA packaging protein
*NC_000913580,4190CG54.2% 98.1 / 112.1 72I121M (ATC→ATGnohBbacteriophage DNA packaging protein
*NC_000913580,5090GC53.5% 144.4 / 166.2 101L151L (CTG→CTCnohBbacteriophage DNA packaging protein
*NC_000913580,5120AG52.4% 134.8 / 149.4 103K152K (AAA→AAGnohBbacteriophage DNA packaging protein
*NC_0009131,634,0260TG50.0% 85.4 / 104.9 54A122A (GCA→GCCnohANohA
*NC_0009131,949,4110AC49.7% 143.8 / inf 499N197H (AAC→CAC) yecDhypothetical protein
*NC_0009131,949,4100CT49.4% 27.2 / inf 501L196L (CTC→CTTyecDhypothetical protein
*NC_0009133,192,6190AC48.5% 151.4 / inf 521intergenic (+78/+342)b3051/b3052putative membrane protein/putative kinase
*NC_0009131,949,3560TC48.2% 76.4 / inf 487S178S (AGT→AGCyecDhypothetical protein
*NC_0009131,949,4360AG48.1% 88.2 / inf 423L6L (CTA→CTGyecEhypothetical protein
*NC_000913580,5130CA47.8% 202.1 / 168.7 111R153R (CGG→AGG) nohBbacteriophage DNA packaging protein

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207013 =177 (0.690)90 (0.420) 66/152 4.4 39.3% coding (290/630 nt) ycfK hypothetical protein
?NC_000913 1208842 = 132 (0.620)coding (40/540 nt) b1157 putative tail fiber protein
* ? NC_000913 = 1207028160 (0.620)88 (0.410) 65/152 4.6 40.8% coding (305/630 nt) ycfK hypothetical protein
?NC_000913 = 1208825 124 (0.580)coding (57/540 nt) b1157 putative tail fiber protein
* ? NC_000913 = 400839874 (0.290)49 (0.200)
+AAGA
21/172 19.0 40.6% coding (481/516 nt) b4555 hypothetical protein
?NC_000913 4008399 = 74 (0.290)coding (482/516 nt) b4555 hypothetical protein
* ? NC_000913 = 1208841193 (0.750)15 (0.070) 10/154 23.3 7.4% coding (41/540 nt) b1157 putative tail fiber protein
?NC_000913 2469917 = 212 (0.980)coding (166/519 nt) yfdL putative RNA polymerase beta
* ? NC_000913 = 28759300 (0.000)4 (0.020)
+G
4/182 30.5 100% intergenic (+289/+662) iap/ygbF Iap/hypothetical protein
?NC_000913 2875993 = 0 (0.000)intergenic (+352/+599) iap/ygbF Iap/hypothetical protein