Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 56,908 C→A V68L (GTG→TTG)  lptD ← LPS assembly OM complex LptDE, beta‑barrel component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927356,9080CA97.0% 93.7 / NA 33V68L (GTG→TTG) lptDLPS assembly OM complex LptDE, beta‑barrel component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (11/21);  minor base G (1/0);  total (12/21)
Fisher's exact test for biased strand distribution p-value = 3.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATCGTCGTAATGGACATTACCGAGCGCATCAACGGTACGTACCGGCTCCGGTTGTCCTGGTGCCTCTTTTTGATGGAGCTGCACTTCGTCGGCCTGCAGACGGCTGTTACCCTGCATGATATCCACGCTGCCAGTAAACACGGCGTCATCCGGGTAGTCCCCTTTCGCGTGGTCAGCATTGATAGTCACGGGTAAGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGG  >  CP009273/56767‑57036
                                                                                                                                             |                                                                                                                                
tATCGTCGTAATGGACATTACCGAGCGCATCAACGGTACGTACCGGCTCCGGTTGTCCTGGTGCCTCTTTTTGATGGAGCTGCACTTCGTCGGCCTGCAGACGGCTGTTACCCTGCATGATATCCACGCTGCCAGTAAACAAGGCGTCa                                                                                                                           <  2:697891/149‑1 (MQ=255)
 aTCGTCGTAATGGACATTACCGGGCGCATCAACGGTACGTACCGGCTCCGGTTGTCCTGGTGCCTCTTTTTGATGGAGCTGCACTTCGTCGGCCTGCAGACGGCTGTTACCCTGCATGATATCCACGCTGCCAGTAAACAAgg                                                                                                                                <  1:78195/143‑1 (MQ=255)
 aTCGTCGTAATGGACATTACCGAGCGCATCAACGGTACGTACCGGCTCCGGTTGTCCTGGTGCCTCTTTTTGATGGAGCTGCACTTCGTCGGCCTGCAGACGGCTGTTACCCTGCATGATATCCACGCTGCCAGTAAACAAgg                                                                                                                                <  1:167453/143‑1 (MQ=255)
 aTCGTCGTAATGGACATTACCGAGCGCATCAACGGTACGTACCGGCTCCGGTTGTCCTGGTGCCTCTTTTTGATGGAGCTGCACTTCGTCGGCCTGCAGACGGCTGTTACCCTGCATGATATCCACGCTGCCAGTAAACAAGGCGTCAt                                                                                                                          >  2:671478/1‑149 (MQ=255)
  tcgtcgTAATGGACATTACCGAGCGCATCAACGGTACGTACCGGCTCCGGTTGTCCTGGTGCCTCTTTTTGATGGAGCTGCACTTCGTCGGCCTGCAGACGGCTGTTACCCTGCATGATATCCACGCTGCCAGTAAACAAGGc                                                                                                                               <  1:80107/143‑1 (MQ=255)
    gtcgtAATGGACATTACCGAGCGCATCAACGGTACGTACCGGCTCCGGTTGTCCTGGTGCCTCTTTTTGATGGAGCTGCACTTCGTCGGCCTGCAGACGGCTGTTACCCTGCATGATATCCACGCTGCCAGTAAACAAGGCGt                                                                                                                             <  1:169471/143‑1 (MQ=255)
    gtcgtAATGGACATTACCGAGCGCATCAACGGTACGTACCGGCTCCGGTTGTCCTGGTGCCTCTTTTTGATGGAGCTGCACTTCGTCGGCCTGCAGACGGCTGTTACCCTGCATGATATCCACGCTGCCAGTAAACAAGGCGt                                                                                                                             >  1:466642/1‑143 (MQ=255)
     tcgtAATTGACATGCCCGAGCGCATCAACGGTACGTACCGGCTCCGGTTGCCCCAGTGCCTCTTTTTGATGGAGCTGCACTTCGTCGGCCTGCAGACGGCTGTTACCCTGCATGATATCCACGCTGCCAGTAAACAAGGCGTCATCCgg                                                                                                                      <  2:339064/149‑1 (MQ=255)
                              ccactgtacgtacCGGCTCCGGTTGTCCTGCTGCCTCTTTTTGATGGAGCTGCACTTCGTCGGCCTGCAGACGGCTGTTACCCTGCATGATATCCACGCTGCCAGTAAACAAGGCGTCATCCGGGTAGTCCCCTTTCGCGTgg                                                                                                   <  1:29016/138‑1 (MQ=255)
                              cAACGGTACGTACCGGCTCCGGTTGTCCTGGTGCCTCTTTTTGATGGAGCTGCACTTCGTCGGCCTGCAGACGGCTGTTACCCTGCATGATATCCACGCTGCCAGTAAACAAGGCGTCATCCGGGTAGTCCCCTCTCGCGTgg                                                                                                   >  1:558744/1‑143 (MQ=255)
                                 cGGTCCGTACCGGCTCCGGTTGTCCTGGTGCCTCTTTTTGATGGAGCTGCACTTCGTCGGCCTGCAGACGGCTGTTACCCTGCATGATATCCACGCTGCCAGTAAACAAGGCGTCATCCGGGTAGTCCCCTTTCGCGTGGTCAGCATTg                                                                                          <  2:407534/149‑1 (MQ=255)
                                    tacgtacCGGCTCCGGTTGTCCTGGTGCCTCTTTTTGATGGAGCTGCACTTCGTCGGCCTGCAGACGGCTGTTACCCTGCATGATATCCACGCTGCCAGTAAACAAGGCGTCATCCGGGTAGTCCCCTTTCGCGTGGTCAGCATTGATa                                                                                       <  2:28779/149‑1 (MQ=255)
                                       gtacCGGCTCCGGTTGTCCTGGTGCCTCTTTTTGATGGAGCTGCACTTCGTCGGCCTGCAGACGGCTGTTACCCTGCATGATATCCACGCTGCCAGTAAACAAGGCGTCATCCGGGTAGTCCCCTTTCGCGTGGTCAGCATTg                                                                                          >  1:288222/1‑143 (MQ=255)
                                         acCGGCTCCGGTTGTCCTGGTGCCTCTTTTTGATGGAGCTGCACTTCGTCGGCCTGCAGACGGCTGTTACCCTGCATGATATCCACGCTGCCAGTAAACAAGGCGTCATCCGGGTAGTCCCCTTTCGCGTGGTCAGCATTGAt                                                                                        >  1:388064/1‑143 (MQ=255)
                                         acCGGCGCCGGTGGTCCTGGTGCCTCTTTGGGATGGAGCTGCACTTCGTCGGCCTGCAGACGGCTGTTACCCTGCATGATATCTACGCTGACAGTAAACAAGGCGTCATCCGGGTAGTCCCCTTTCGCGTGGTCAGCATTGATAGTCAc                                                                                  <  2:322788/149‑1 (MQ=255)
                                           ccggtcagtgcctCCTTGTGCCTCTTTTTGATGGAGCTGCACTTCGTCGGCCTGCAGACGGCTGTTACCCTGCATGATATCCACGCTGCCAGTAAACAAGGCGTCATCCGGGTAGTCCCCTTTCGCGTGGTCAGCATTGATAg                                                                                      >  1:6001/13‑143 (MQ=255)
                                             gCTCCGGTTGTCCTGGTGCCTCTTTTTGATGGAGCTGCACTTCGTCGGCCTGCAGACGGCTGTTACCCAGCATGATATCCACGCTGCCAGTAAACAGGGCGTCATCCGGGTAGTCCCCTTTCGCGTGGTTAGCATTGATAGTCACGGGt                                                                              >  2:520901/1‑149 (MQ=255)
                                                ccGGTTGTCCTGGTGCCTCTTTTTGATGGAGCTGCACTTCGTCGGCCTGCAGAAGGATGTTGCGCTGCGTGATCTACATCGTGTCTTTAAACAAGGCGTCATCCGGGTGGTCCCCTTTCGCGTGGTCAGCTTTGATAGTCACGGGTAAg                                                                           >  2:342454/1‑149 (MQ=255)
                                                      gTCCTGGTGCCTCTTTTTGATGGAGCTGCACTTCGTCGGCCTGCAGACGGCTGTTACCCTGCATGATATCCACGCTGCCAGTAAACAAGGCGTCATCCGGGTAGTCCCCTTTCGCGTGGTCAGCATTGATAGTCACGGGTAAGTCATTg                                                                     <  2:7081/149‑1 (MQ=255)
                                                               ccTCTTTTTGATGGAGCTGCACTTCGTCGGCCTGCAGACGGCTGTTACCCTGCATGATATCCCCGCTGCCAGTAAACAAGGCGTCATCCGGGTAGTCCCCTTTCGGGTGGGCAGCCTAGATAGTACCggggccgccactggca                                                                  >  1:43015/1‑130 (MQ=255)
                                                                ctctTTTTGATGGAGCTGCACTTCGTCGGCCTGCAGACGGCTGTTACCCTGCATGATATCCACGCTGCCAGTAAACAAGGCGTCATCCGGGTAGTCCCCTTTCGCGTGGTCAGCATTGATAGTCACGGGTAAGTCATTGGTATCGGCCt                                                           >  2:436029/1‑149 (MQ=255)
                                                                  ctTTTTGATGGAGCTGCACTTCGTCGGCCTGCAGACGGCTGTTACCCTGCATGATATCCACGCTGCCAGTAAACAAGGCGTCATCCGGGTAGTCCCCTTTCGCGTGGTCAGCATTGATAGTCACGGGTAAGTCATTGGTATCGCCCTGt                                                         <  2:376479/149‑1 (MQ=255)
                                                                   tttttGATGGAGCTGCACTTCGTCGGCCTGCAGACGGCTGTTACCCTGCATGATATCCACGCTGCCAGTAAACAAGGCGTCATCCGGGTAGTCCCCTTTCGCGTGCTCCGCCTTGATACCCACGCGTAAGTCATTGGTATCGc                                                              <  1:638328/143‑1 (MQ=255)
                                                                       tGATGGAGCTGCACTTCGTCGGCCTGCAGACGGCTGTTACCCTGCATGATATCCACGCTGCCAGTAAACAAGGCGTCATCCGGGTAGTCCCCTTTCGCGTGGTCAGCATTGATAGTCACGGGTAAGTCATTGGTATCGCCCTg                                                          >  1:586358/1‑143 (MQ=255)
                                                                       ggATGGAGCTGCACGTCGCCGGCCTGCAGACGGCTGTGACCCGGCATGATATCCACGCTGCCAGTAAACAAGGCGTCATCCGGGTAGTCCCCTTTCGCGTGGTCAGCATTGATAGTCACGGGTAAGTCATTGGTATCGCCCTg                                                          <  1:35701/142‑1 (MQ=255)
                                                                         aTGGCGCTGCACTGCGTCGGCCTGGAGCCGGCTGTTCCCCTGCATGATATCCACGCTGCCAGTAAACAAGGCGTCATCCGGGTAGTCCCATTTCGCGTGGTCAGCATTGATAGTCACGGGTAAGTCATTGGTATCGCCCTGTa                                                        <  1:338889/143‑1 (MQ=255)
                                                                                             ccTGCAGACGGCTGTTACCCTGCATGATATCCACGCTGCCAGTAAACAAGGCGTCATCCGGGTAGTCCCCTTTCGCGTGGTCAGCATTGATAGTCACGGGTAAGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAGCtt                                    <  1:615888/143‑1 (MQ=255)
                                                                                                     cGGCTGTTACTCTTCATGATATCCACGCTGCCAGTAAACAAGGCGTCATCCGGGTAGTCCCCTTTCGCGTGGTCAGCATTGATAGTCACGGGTAAGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACAt                      <  2:579859/149‑1 (MQ=255)
                                                                                                       gCTGTTTCCCTGCAGGTTTTCCACGCTGCCAGCAAACAAGGCGTCATCCGGGTAGTCCCCTTTCGCGTGTTCAGCATTGATATTCACGGGTAAGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCa                          <  1:161353/143‑1 (MQ=255)
                                                                                                                   cATGATATCCACGCTGCCAGTAAACAAGGCGTCATCCGGGTAGTCCCCTTTCGCGTGGTCAGCATTGATAGTCACGGGTAAGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGa        <  2:479647/149‑1 (MQ=255)
                                                                                                                        tatCCACGCTGCCAGTAAACAAGGCGTCATCCGGGTAGTCCCCTTTCGCGTGGTCAGCATTGATAGTCACGGGTAAGTCATTGGTCTCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCg         <  1:279479/143‑1 (MQ=255)
                                                                                                                          tCCACGCTGCCAGTAAACAAGGCGTCATCCGGGTAGTCCCCTTTCGCGTGGTCAGCATTGATAGTCACGGGTAAGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAg       <  1:310070/143‑1 (MQ=255)
                                                                                                                               gCTGCCAGTAAACAAGGCGTCATCCGGGTAGTCCCCTTTCGCGTGGTCAGCATTGATAGTCACGGGTAAGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAACTTGGCACGCCCAACAGGCACTGTGAGGCGAGGTCgg  >  1:32875/1‑143 (MQ=255)
                                                                                                                                             |                                                                                                                                
TATCGTCGTAATGGACATTACCGAGCGCATCAACGGTACGTACCGGCTCCGGTTGTCCTGGTGCCTCTTTTTGATGGAGCTGCACTTCGTCGGCCTGCAGACGGCTGTTACCCTGCATGATATCCACGCTGCCAGTAAACACGGCGTCATCCGGGTAGTCCCCTTTCGCGTGGTCAGCATTGATAGTCACGGGTAAGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGG  >  CP009273/56767‑57036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑