| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,667,677 | G→A | 41.9% | A124V (GCG→GTG) | yphB ← | mutarotase superfamily protein, YphB family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,667,677 | 0 | G | A | 41.9% | 8.5 / 17.0 | 23 | A124V (GCG→GTG) | yphB | mutarotase superfamily protein, YphB family |
| Reads supporting (aligned to +/- strand): ref base G (6/7); new base A (6/3); total (12/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 | |||||||||||
ACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCA > CP009273/2667531‑2667819 | aCTGCTCCCGCTCCAGCCAGTAACCGAAGGGCGGTCCCAAAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCCATGCCCCTTGATTGGTGGCACACAGCGTCACCGTCACCGTATccgcc > 2:175082/1‑149 (MQ=255) cccGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGCGAGCGTCACCGTCAGCGTAGCCGCCGTTaaa > 1:307205/1‑149 (MQ=255) ccGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACGGTTAAAt > 2:182913/1‑149 (MQ=255) cGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGCATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGGCAGAGGGCGTCACCGTCAGCGTATCCGCCGTTAAATg > 2:174588/1‑149 (MQ=255) tCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGaaa < 2:94888/149‑1 (MQ=255) cGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGGACCAAGTGGCAGCGTCTCTGCCCCTTGATGGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTGCATGAAACGCCTGACTGAcc > 2:146513/1‑149 (MQ=255) cGCTCGCCTGTGCCCGAGTCCGCTTTGGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTAcc < 2:7147/149‑1 (MQ=255) cTGAACCCGCGTTTGCGACGGAGACCGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACATAGAGCGTCACCGTCAGCGTATCCGCTGTTAAATTAAACGCCTGACTTACCCGATAGTGATAGa < 2:232651/149‑1 (MQ=255) gAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTATGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTCAATAAAACCCCTGACTGACCCGCTaaatatataca > 2:117409/1‑139 (MQ=255) ccGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGc < 1:281274/149‑1 (MQ=255) cGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATTGTGATAGACACCGCTGCGATGTTCATACACCa < 1:256567/149‑1 (MQ=255) aGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAAc > 1:135384/1‑149 (MQ=255) ccGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAACGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCt < 2:226414/149‑1 (MQ=255) aaTGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATATTCATCCCCAAAACACAAACTatcaccccccagttagac > 2:82078/1‑135 (MQ=255) tGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCCTCGCTGTGCGAGacac < 2:53413/149‑1 (MQ=255) cGTCCCTGCCCCTTGATTGGTGACAGAGAGCGGCCCCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGcc < 1:182913/149‑1 (MQ=255) ctctGCCCCTTGATTGGTGACAGAGAGCGGCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACt < 1:167264/149‑1 (MQ=255) tctGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTc > 2:231498/1‑149 (MQ=255) tGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCACCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGc > 2:202359/1‑149 (MQ=255) ccccTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGGGCCACATTGCCACTCGccc > 2:141698/1‑149 (MQ=255) cccTTGATTGGTGACAGAGAGCGACACCGTGACCGTAGCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCcca > 1:345136/1‑149 (MQ=255) tGATTGGTGACGGAGAGCGTCACCGCCAGCGTATCCCCCGTTATATGAAACTCCTGCCTTACCCGATAGTGATAGACACCGCTGCCATGTTCATACACCAGACACAATCTATCATCGCTGTGCGAGACACATTGCCCCTCGCccagcca < 1:146513/149‑1 (MQ=255) aCCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCAGCGCCGGGTAGATAGTg > 1:268530/1‑149 (MQ=255) gTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCa < 2:247444/149‑1 (MQ=255) | ACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCGCCCAGCCAGCCATCGCCGTGTAGATAGTGCGCATCCCA > CP009273/2667531‑2667819 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |