| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,111,161 | C→A | 49.7% | R293L (CGT→CTT) | lpxL ← | lauryl‑acyl carrier protein (ACP)‑dependent acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,111,161 | 0 | C | A | 49.7% | ‑6.1 / 24.6 | 22 | R293L (CGT→CTT) | lpxL | lauryl‑acyl carrier protein (ACP)‑dependent acyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (6/5); new base A (8/3); total (14/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01 | |||||||||||
GCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATT‑TAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGG > CP009273/1111014‑1111306 | gcCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGG‑ATAGAAGTCTCTAAGGGCGTGACGAAAGGCCTACCGTGCGTGTTTTAAAGCGACg > 2:349602/1‑149 (MQ=255) ccGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATT‑TAATAGCGTGAAGGAACGCCTTCCGGGCGGGTTTTAAAGCGAAGgt > 2:357719/1‑149 (MQ=255) agagagCTTTTATCGCTAAATCAGGGGGATTTGCGGTGGTAATGCCGGATGCCATTCTGAAGCATCCAGCATGGGAGATT‑TAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTggg > 1:12837/1‑148 (MQ=255) agagCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATT‑TAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGc > 2:113916/1‑149 (MQ=255) gagCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATT‑TAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGcc > 2:295708/1‑149 (MQ=255) aGGGGGATTTGCTGTGGTAAGGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATT‑TAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGa < 2:166857/149‑1 (MQ=255) ttGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATT‑TAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTg > 2:178042/1‑149 (MQ=255) tgGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATT‑TAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATc > 1:230952/1‑149 (MQ=255) ccGGATGCCATTCTGAAGCATCCGGCATGGGAGATT‑TAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGAACACTTTGTTCATCCaaccgtc > 1:332839/1‑143 (MQ=255) gCCATTCTGAAGCATCCGGCATGGGAGATT‑TAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGt > 2:328177/1‑149 (MQ=255) gCATCCGGCATGGGAGATT‑TAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGcatcat > 2:325100/1‑149 (MQ=255) gCATCCGGCATGGGAGATT‑TAATAGCGTGAAGGAACACCTTCCGGGCGGGTATTAAAGCGAAGGTGTAACCAAATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGAGGTAGTTTCGGcatcat > 2:164820/1‑149 (MQ=255) gCATGGGAGATT‑TAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGGGGCGGTAGTTTCGGCATCATCCGGTgg > 2:314090/1‑149 (MQ=255) gggAGTTT‑TAAGAGCGTGAGGGAACGCCTGCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGga < 2:7708/149‑1 (MQ=255) aTT‑TAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAaca < 2:332774/149‑1 (MQ=255) tAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTgg < 1:113916/149‑1 (MQ=255) aCGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTTTTTTTTTTTTTTCATCCACGCGGCGGTACGTTCAGCATCGTCCAGTGGGGGGGAACACTGTGGCTGCCGCATAAt > 1:107360/1‑149 (MQ=255) cGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATc < 2:230952/149‑1 (MQ=255) ctccctggCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAAt < 1:125864/143‑1 (MQ=255) tCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGAAGCATAATCGATTg > 2:63484/1‑149 (MQ=255) cGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTgg < 2:338320/149‑1 (MQ=255) cGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTgg < 1:46307/149‑1 (MQ=255) | GCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATT‑TAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGG > CP009273/1111014‑1111306 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |