| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,154,028 | G→A | 36.1% | G235D (GGC→GAC) | ptsG → | fused glucose‑specific PTS enzymes: IIB component/IIC component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,154,028 | 0 | G | A | 36.1% | 10.0 / 11.2 | 21 | G235D (GGC→GAC) | ptsG | fused glucose‑specific PTS enzymes: IIB component/IIC component |
| Reads supporting (aligned to +/- strand): ref base G (7/6); new base A (3/4); total (10/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01 | |||||||||||
CCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTAT > CP009273/1153895‑1154175 | ccGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTGGGGCTGCACCGCATCTGGAACGTACCTTTCCGGGTGCCGATTGGTGAATAAACAAACGCAGCGGGGCAGCTTGTCCACGGCGACATTcaccggga > 1:186886/1‑142 (MQ=255) ttGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTCCCTTTCCAGATGCAGATTGGTGCATACACCAACGCAGCAGGTCCGGTTTTCCACGGCGACATTCCCCGTTATATGGCGGGAGAc > 2:162773/1‑149 (MQ=255) ttGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGTCATTCCGCGTTATATGGCGGGTGAc < 1:122341/149‑1 (MQ=255) cGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGCCGACATTCCGCGTTCTATGGCGGGTGACCCGACTGCgg < 2:192773/149‑1 (MQ=255) cGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCgg < 2:112830/149‑1 (MQ=255) tttCATTGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTa < 2:39528/149‑1 (MQ=255) gtccTGGTACCGGTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCACCGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCtggtgg < 2:69916/147‑1 (MQ=255) ggTCTGCATCACATCTTGAACTGACTTTGTCATATGCAGATTGGGGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAAt < 1:178370/149‑1 (MQ=255) tGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCACAATGTAc > 2:301958/1‑149 (MQ=255) gCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGGAAACTGTCTGGTGACTACCTGTTCAAAATGTACg > 2:104514/1‑149 (MQ=255) catcctgcaCGTACCTTTCCAGATCCAGATTGGTTAATACACCAACGCCTCAGGTCAGTTTTCCCGCGGCGACATTCCGCTGTATACGGCGGGCGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGc < 1:272535/141‑1 (MQ=255) cTGGAACGTACCTTTCCAGCTGCAGATTGGTGAATACACCTACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAg < 1:311652/149‑1 (MQ=255) gAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTg < 2:115585/149‑1 (MQ=255) aCCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCaa > 2:61308/1‑149 (MQ=255) aTTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGACGACATTCCGCGTTAGATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGTTGGCTTCCTGGTAAAGATGTACGATTTGCCAGCTGCCGCCATTGCTATCTCGGActc > 2:66844/1‑149 (MQ=255) acCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTCCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACCCTGCTAAACCAGa > 1:179307/1‑149 (MQ=255) cAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGaaa > 2:342743/1‑149 (MQ=255) agcagGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACcgcg < 2:325409/149‑1 (MQ=255) gtcaggtTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGaaa < 1:61308/149‑1 (MQ=255) ggtTTTCCACGACGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAACCCAGAAAACCGCGCGAAAGTgg > 2:120082/1‑149 (MQ=255) tCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCGTCCTGTTCAAAATGTACGGTCTACCGGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCCGAAAGCCGGGCGAAAGTGGGCGGt > 1:229777/1‑149 (MQ=255) ggCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGtattat > 2:177492/1‑149 (MQ=255) | CCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCGCGAAAGTGGGCGGTATTAT > CP009273/1153895‑1154175 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |