| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,083,827 | G→C | 37.1% | R52P (CGC→CCC) | hisG → | ATP phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,083,827 | 0 | G | C | 37.1% | 17.7 / 14.7 | 22 | R52P (CGC→CCC) | hisG | ATP phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (5/8); new base C (5/3); total (11/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTGGCGCGCTGTGGCATTAAAATTAATCTTCACACCCAGCGCCTGATCGCGATGGCAGAAAACATGCCGATTGATATTCTGCGCGTGCGTGACGACGACATTCCCGGTCTGGTAATGGATGGCGTGGTAGACCTTGGGATTATCGGCGAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGG > CP009273/2083686‑2083970 | cTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTGGAGCGCTGTGGCATTAAAATTAATCTTCACACCCAGCGCCTGATCGCGATGGCAGAAAACATGCCGATTGATATTCTGCgcgtgcgt > 2:232617/1‑149 (MQ=255) gCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCAAGGGTTTTGCTTGCGCGCTGTGTGATAATAATTAATCTTCACACCCAGCGCCTGATCGCGATGGCAGAAAACATGCCGATTGATATTCTGCTCGTGCGTgacgacgac > 2:352911/1‑149 (MQ=255) cTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTGGCGCGCTGTGGCATTAAAATTAATCTTCACACCCAGCGCCTGATCGCGATGGCAGAAAACATGCCGATTGATATTCTGCCCGTGCGTGACGACGACATTccc > 2:254206/1‑149 (MQ=255) tGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTGGCGCGCTGTGGCATTAAAATTAATCTTCACACCCAGCGCCTGATCGCGATGGCAGAAAACATGCCGAGTGATATTCTGCCCGTGCGTGACGACGACATTCCCGGt < 2:196346/149‑1 (MQ=255) cAGAACTCCGGCCGTTTAAGTGAGGACTCACGCGAATTGCTGGCGCGCTGTGGCATTAAAATTAATCTTCCCTCCCAGCGCCTGATCGCGATGGCAGAAAACATGCCGATTGATATTCTGCGCGTGCGTGACGACGACATTCCCGGTCt < 1:69884/149‑1 (MQ=255) cAGAAATCCGGCCGTTTAAGTGCTGACCCACGCGAATTGCTGGCGCGCTGTGGCATTAAAATTAATCTTCACACGCCGCGCCTGATCGCGATGGCAGAAAACATGCCGATTGATATTCTGCGCGTGCGTGACGACGACATTCCCGGTCt < 2:104967/149‑1 (MQ=255) tAAGTGATGACTCACGCGAATTGCTGGCGCGCTGTGGCATTCAAATTAATCTTCACACCCAGCGCCTGATCGCGATGGCAGAAAACATGCCGATTGATATTCTGCCCGTGCGTGACGACGACATTCCCGGTCTGGTAATGGATGGCGTg > 2:227167/1‑149 (MQ=255) tCACGCGAATTGCTGGCGCGCTGTGGCATTAAAATTAATCTTCACACCCAGCGCCTGATCGCGATGGCAGAAAACATGCCGATTGATATTCTGCGCGTGCGTGACGACGACATTCCCGGTCTGGTAATGGATGGCGTGGTAGACCTTgg < 1:339991/149‑1 (MQ=255) aCGCGAATTGCTGGCGCGCTGTGGCATTAAAATTAATCTTCACACCCAGCGCCTGATCGCGATGGCAGAAAACATGCCGATTGATATTCTGCGCGTGCGTGACGACGACATTCCCGGTCTGGTAATGGATGGCGTGGTAGACCTTGGGa > 2:118778/1‑149 (MQ=255) aCGCGAAGTGCTGGCGCGCTGTGGCACTAAAATTAATCTTCACACCCACCGCCTGATCGCGATGGCAGAAAACATGCCGATTGATATTCTGCGCGTGCGTTACGACGACAGTCCCGGTCTGGTTATGGATGGCGTGGTAGACCTTGGGa < 2:90632/149‑1 (MQ=255) ccgcgcTGTGGCATTAAAATTAATCTTCACACCCTGCGCCTGATCGCGATTGCAGAAAACATGCCGATTGATATTCTGCGCGTGCGTGACGACGACATTCCCGGTCTGGTAATGGATGGCGTGGTAGACCTTGGGATTATCGGCGaaaa < 2:75240/148‑1 (MQ=255) gtgGCATTAAAATTAATCTTCACACCCAGCGCCTGATCGCGATGGCAGAAAACATGCCGATTGATATTCTGCCCGTGCGTGACGACGACATTTCCGGTCCGGTAATGGATGGCGTGGTAGACCTTGGGATTATCGGCGAAAACGTGCTg < 1:233055/149‑1 (MQ=255) cATTAAAATTAATCTTCACACCCAGCGCCTGATCGCGATGGCAGAAAACATGCCGATTGATATTCTGCGCGTGCGTGACGACGACATTCCCGGTCTGGTAATGGATGGCGTGGTAGACCTTGGGATTATCGGCGAAAACGTGCTGgaag < 1:313543/149‑1 (MQ=255) cATGAAAATTAATCTTCACACCCAGCGCCTGATCGCGATGGCAGAAAACATGCCGATTGATATTCTGCGCGTGCGTGACGACGACATTCCCGGTCTGGTAATGGATGGCGTGGTAGACCTTGGGATTATCGGCGAAAACGTGCTGgaag < 1:136486/149‑1 (MQ=255) ttAAAATTAATCTCCACACCCAGCGCCTGATCGCGATGGCAGAAAACATGCCGATTGATATTCTGCGCGTGCGTGACGACGACATTCCCGATCTGGGTCGGGATGTCGTGGTAGGCCTTGGGATTATCGGCGAAAACGTGCTGgaagaa > 2:244428/1‑149 (MQ=255) aaaTTAATCTTCACACCCAGCGCCTGATCGCGATGGCAGAAAACATGCCGCTTGATATTCTGCCCGTGCGTGCCGACGACATTCCCGGACCGGTAATGGATGGCGTGGCGGCGCTTGGGATTATCGGCCGAACCGTGCGGGAAGGagag > 1:91823/1‑149 (MQ=255) aTTAATCTTCACACCCAGCGCCTGATCGCGATGGCAGAAAACATGCCGATTGATATTCTACGCGTGCGTGACGACGACATTCCCGGTCTGGTAATGGATGGCGTGGTAGACCTTGGGATTATCGGCGAAAACGTGCTGGAAGAAGAgct > 2:237398/1‑149 (MQ=255) cTTCACCCCCAGCGCCTGATCGCGATGGCAGAAAACAAGCCCGTTGATGTTCTGCGCGTGCGTGACGACGACATTCCCGGTCTGGTAATGGATGGCGTGGTAGACCTTGGGATTATCGGCGAAAACGTGCTGGAAGAAGAGCTGCTTaa < 2:294601/149‑1 (MQ=255) tCACACCCAGCGCCTGATCGCGATGGCAGAAAACATGCCGATTGATATTCTGCGCGTGCGTGACGACGACATTCCCGGTCTGGTAATGGATGGCGTGGTAGACCTTGGGATTATCGGCGAAAACGTGCTGGAAGAAGAGCTGCTTAAcc > 1:62432/1‑149 (MQ=255) tCGCGATGGCAGAAAACATGCCGATTGATATTCTGCGCGTGCGTGACGACGACATTCCCGGTCTGGTAAGGGATGGCGTGGTAGACCTTGGGATTATCGGCGAAAACGTGCTGGAAGAAGAGCTGCTTACCCGCCGCGCCCAGGGTgca > 2:168431/1‑147 (MQ=255) cgATGGCAGAAAACATGCCGATTGTTATTCTGCCCGTGCGTGACGACGACATTCCCGGTCTGGTAATGGATGGCGTGGTAGACCTTGGGATTATCGGCGAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGAt < 1:93819/149‑1 (MQ=255) aaaaCATGCCGATTGATATTCTGCCCGTGCGTGACGACGACATTCCCGGTCTGGTAATGGATGGCGTGGTAGACCTTGGGATTATCGGCGAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTAc > 1:227762/1‑149 (MQ=255) tCTGCCCGTGCGTGACGACGACAGTCCCGGGATGGTAATGGAGAGCGTGGTAGACGTTGGGATTATCGGCGGAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGGCCAGGAGTAACATCCCCGCTCCTTTACCCTGcctcgcccgc > 1:177380/1‑144 (MQ=255) | CTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTGGCGCGCTGTGGCATTAAAATTAATCTTCACACCCAGCGCCTGATCGCGATGGCAGAAAACATGCCGATTGATATTCTGCGCGTGCGTGACGACGACATTCCCGGTCTGGTAATGGATGGCGTGGTAGACCTTGGGATTATCGGCGAAAACGTGCTGGAAGAAGAGCTGCTTAACCGCCGCGCCCAGGGTGAAGATCCACGCTACTTTACCCTGCGTCGTCTGG > CP009273/2083686‑2083970 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |