Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 982,565 T→A intergenic (‑127/+476) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273982,5650TA98.4% 204.5 / NA 65intergenic (‑127/+476)ompF/asnSouter membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  major base A (36/27);  minor base G (1/0);  total (38/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCA  >  CP009273/982421‑982708
                                                                                                                                                 |                                                                                                                                               
aaTATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACaaa                                                                                                                                             <  2:881915/149‑1 (MQ=255)
 atatTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAAc                                                                                                                                            <  1:953153/149‑1 (MQ=255)
      gcgcTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGTCACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCCTCTTTCCGTACAAACTAAct                                                                                                                                       >  2:753010/1‑148 (MQ=255)
       cgcTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAAcct                                                                                                                                      >  2:620790/1‑147 (MQ=255)
       cgcTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGt                                                                                                                                      <  1:238833/149‑1 (MQ=255)
         cTTCATCATTATTTATTTCCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGttt                                                                                                                                    <  1:252784/149‑1 (MQ=255)
         cTTCATCATTATTTATTACCTTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGttt                                                                                                                                    <  1:1036085/149‑1 (MQ=255)
         cTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGttt                                                                                                                                    <  1:468014/149‑1 (MQ=255)
          ttCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCACTACGAATTTAGTGGAGTATTGTTGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCATACTAACGTTTc                                                                                                                                   >  2:369088/1‑149 (MQ=255)
            catcatTATTTATTACCCTCATGGTTTTTTTTATTATACCTGCCACTGCCGTCAATAAGATCTGTCAATAAAGATTTACGGAACTATTGATGAGGGTTTGGTGTCTTTATGTGTCTTCTTGCATCTTTCCATATAAACTATTGTTTctc                                                                                                                                 >  2:1099016/1‑147 (MQ=255)
              tcatTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCAACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTa                                                                                                                               >  1:1043650/1‑149 (MQ=255)
                  tatttattACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGCCCGTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGt                                                                                                                           <  1:1367219/149‑1 (MQ=255)
                           cccctctGGTTTTCTTTTATGACACCCGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGc                                                                                                                   <  1:470854/143‑1 (MQ=255)
                           cccTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCt                                                                                                                  <  1:1213082/149‑1 (MQ=255)
                             cTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCATTAGTTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTAc                                                                                                                >  1:1057775/1‑149 (MQ=255)
                              tCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACa                                                                                                               >  1:719247/1‑149 (MQ=255)
                               cttGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACaa                                                                                                              <  2:216341/147‑1 (MQ=255)
                                   gTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagat                                                                                                          >  1:129953/1‑149 (MQ=255)
                                              tGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTgg                                                                                                >  2:1045613/1‑149 (MQ=255)
                                                    cTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaa                                                                                          >  1:1369306/1‑149 (MQ=255)
                                                      gCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaaaa                                                                                        >  1:794183/1‑149 (MQ=255)
                                                      gCCACTGCCGTCAATAAGTTCTGTCAAAAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCAACAGGCAGCTTTCCACACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaaaa                                                                                        <  1:566273/149‑1 (MQ=255)
                                                      gCCACCGCCGCCAATAATTTCTGTCAATAACAATTTCCGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAAGCATCTTTCCAGACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaaaa                                                                                        <  1:798711/149‑1 (MQ=255)
                                                               gTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAAAGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAat                                                                               >  2:961079/1‑149 (MQ=255)
                                                                   aTAAGTTCTGTCAATAAAACTTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATAAGt                                                                           >  2:279085/1‑149 (MQ=255)
                                                                    tAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTa                                                                          >  1:222722/1‑149 (MQ=255)
                                                                        ttCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCa                                                                      >  2:651692/1‑149 (MQ=255)
                                                                           aatCAATAAAAACTTACGGAACAAATAAAAAAAAATAAGTTTCTTTACCTAACAGCAGGCAGATTTCCACACACACTAACCTTTCGCCACCGTAAAAGTGCTACAACGATAAAGATTTGAAAAAAAAAAAAAAAAATATGTAACCaaaa                                                                   <  2:1265641/147‑1 (MQ=255)
                                                                            gTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACTAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAg                                                                  >  1:556746/1‑149 (MQ=255)
                                                                                 tgaaaaTGTACGGAGCTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTaaaa                                                             <  1:871636/147‑1 (MQ=255)
                                                                                   aaaaTTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataaagat                                                                                                    <  2:413282/108‑1 (MQ=255)
                                                                                   aaaaTTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataaagat                                                                                                    >  1:413282/1‑108 (MQ=255)
                                                                                      aTTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTaa                                                        <  1:1099016/149‑1 (MQ=255)
                                                                                            ggAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACt                                                  <  1:269444/149‑1 (MQ=255)
                                                                                            ggAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACAGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAACTAACATTTAAGGAACt                                                  >  2:400859/1‑149 (MQ=255)
                                                                                              aaCTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACttt                                                >  2:1200160/1‑149 (MQ=255)
                                                                                              aaCTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACttt                                                >  1:880631/1‑149 (MQ=255)
                                                                                                cTATTGAGGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCCACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTtgt                                              <  1:966747/149‑1 (MQ=255)
                                                                                                  aTTGATGAGAGTTTGGTGTCTTTATGTGTCTCCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGACAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGa                                            <  1:976199/149‑1 (MQ=255)
                                                                                                   ttGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATGCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGaa                                           >  2:1086232/1‑149 (MQ=255)
                                                                                                   ttGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGaa                                           <  2:794183/149‑1 (MQ=255)
                                                                                                    tgatgaGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAac                                          <  1:51166/149‑1 (MQ=255)
                                                                                                       tgaGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAATTTTGTGAACAcc                                       >  2:439336/1‑149 (MQ=255)
                                                                                                          gagTCTGCTGTCTTTATGTGTCTGCGGGCATCTTTCCATACAAACTAGCGTTTCGCGACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTc                                    <  2:183080/149‑1 (MQ=255)
                                                                                                             tttGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCata                                 >  1:1271078/1‑149 (MQ=255)
                                                                                                             tttCGTGTCTTTATGTGTTTGCAGGCATCTTTCCATACAAACGAACGTTTCGCTACCGTGAAAGTGCTACAAAGACAAAGATGTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTTAGGAACTTTGTGAACACCGTCata                                 <  1:724949/149‑1 (MQ=255)
                                                                                                                  tgtCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTcc                            >  1:1112241/1‑149 (MQ=255)
                                                                                                                   gtCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCa                           >  2:401754/1‑149 (MQ=255)
                                                                                                                   gtCTTTAGGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCa                           <  2:860119/149‑1 (MQ=255)
                                                                                                                    tCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACCAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCACAGTTCAAt                          >  2:1351749/1‑149 (MQ=255)
                                                                                                                    tCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCAt                          >  1:300553/1‑149 (MQ=255)
                                                                                                                    tCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCAt                          >  1:1224218/1‑149 (MQ=255)
                                                                                                                      tttATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTAATATTTCCATag                        >  1:775912/1‑149 (MQ=255)
                                                                                                                        tATGTGTCTGCAGGCATCTTTCCATACAAACTAACGCTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATagag                      >  1:297255/1‑149 (MQ=255)
                                                                                                                         aTGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAgaga                     >  2:1342333/1‑149 (MQ=255)
                                                                                                                         aTGTGTCTGCAGGCATCTTTCCATACAAACTAACGCTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAgaga                     <  2:297255/149‑1 (MQ=255)
                                                                                                                          tgtgTCTGCAGGCATCTTTCCATACAAACTAACGTCTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGAc                    >  2:876990/1‑149 (MQ=255)
                                                                                                                             gcctgccgacttCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGtg                 >  2:382459/12‑149 (MQ=255)
                                                                                                                                 gCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGtgatga             <  1:620790/149‑1 (MQ=255)
                                                                                                                                     gCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATAtt         >  2:503681/1‑149 (MQ=255)
                                                                                                                                     gCATCCTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATAtt         <  2:1356567/149‑1 (MQ=255)
                                                                                                                                       aTCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTa       >  2:348104/1‑149 (MQ=255)
                                                                                                                                        tCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGAGGTGGTGATATTTAc      >  1:54596/1‑149 (MQ=255)
                                                                                                                                           ttCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGc   >  2:81521/1‑149 (MQ=255)
                                                                                                                                            tCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAAAACCGTCATATTTACATAGCGACGTGATGATATTTACAGCa  >  2:26913/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
AATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCA  >  CP009273/982421‑982708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: