Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	256,243	A→G	6.4%	T49A (ACG→GCG)	proB →	gamma‑glutamate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	256,243	0	A	G	6.4%	175.8 / 2.1	70	T49A (ACG→GCG)	proB	gamma‑glutamate kinase
Reads supporting (aligned to +/- strand):  ref base A (33/30);  new base G (2/3);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01

ATCATGAGTGACAGCCAGACGCTGGTGGTAAAACTCGGCACCAGTGTGCTAACAGGCGGATCGCGCCGTCTGAACCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGTAGGGCAGAGTCGACTGATTCAACTGTGGGAACAGCTGTTTTCGATTTATGGCATT  >  CP009273/256096‑256386                                                                                                                                                    |                                                                                                                                                aTCATGGGTGACAGCCAGACGCTGGTGGTAAAACTCGGCACCAGTGTGCTAACAGGCGGATCGCGCCGTCTGAACCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGAc                                                                                                                                                <  1:400019/149‑1 (MQ=255)    aTGAGTGACAGCCAGACGCTGGTGGTAAAACTCGGCACCAGTGTGCTAACAGGCGGATCGCGCCGTCTGAACCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCACCGGATTGTTATTGTGACGTc                                                                                                                                             >  1:1035654/1‑149 (MQ=255)         tGACAGCCAGACGCTGGTGGTAAAACTCGGCACCAGTGTGCTAACAGGCGGATCGCGCCGTCTGAACCGTGCGCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGt                                                                                                                                              >  2:829234/1‑143 (MQ=255)             aGCCAGACGCTGGTGGTAAAACTCGGCACCAGTGTGCTAACAGGCGGATCGCGCCGTCTGAACCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGGCGTCGGGCGCGAt                                                                                                                                    >  1:1181398/1‑149 (MQ=255)             aGCCAGACGCTGGTGGTAAAACTCGGCACCAGTGTGCTAACAGGCGGATCGCGCCGTCTGAACCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGGCGTCGGGCGCGAt                                                                                                                                    >  1:534732/1‑149 (MQ=255)              tccgaaCGCCGGCGGTCAAACTCGCCACCAGTGTGGTAACAGGCGGATCGCGCCGTCTGCACCGTGCCCATATCGTTGCCCTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATc                                                                                                                                   <  1:572147/144‑1 (MQ=255)               ccagaaaatggtcgTAAAACTCGGCCCCAGTGTGCTAACAGGCGGATCGCGCCGTCTGAACCGTGCCCATATCGTTGAACTTGTTCGCCAGGGCGCGCCGTTACAAGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGgcg                                                                                                                                        >  2:135480/9‑143 (MQ=255)               ccAGACGCTGGTGGTAAAACTCGGCACCAGTGTGCTAACAGGCGGATCGCGCCGTCTGAACCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGCTTGTTATTGTGACGTCGGGCGCGATcg                                                                                                                                  >  1:974168/1‑149 (MQ=255)               ccAGACGCTGGTGGTAAAACTCGGCACCAGTGTGCTAACAGGCGGATCGCGCCGTCTGAACCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGCTCGCTATTGTGACGTCGGgcg                                                                                                                                        >  2:12864/1‑143 (MQ=255)               ccAGACGCTGGTGGTAAAACTCGGCACCAGTGTGCTAACAGGCGGATCGCGCCGTCTGAACCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTGGGGCGCGATcg                                                                                                                                  >  1:759200/1‑149 (MQ=255)                 aGACGCTGGTGGTAAAACTCGGCACCAGTGTGCTAACAGGCGGATCGCGCCGTCTGAACCATGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACCTGCTGCGCGGCATCGGATTGTTATTGTGACGTCGGGcgcg                                                                                                                                      >  2:443806/1‑143 (MQ=255)                    cgggggtggtAAAACGCGGCACCAGTGTGCTAACAGGCTGACCGCGCCGTCTGAACCGTGCCGATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCgccgcc                                                                                                                             <  1:241318/145‑1 (MQ=255)                    cGCTGGTGGTAAACCGCGGCACTAGTGTGCTAACAGGCGGATCGCGCCGTCTGAACCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATc                                                                                                                                   <  2:736947/143‑1 (MQ=255)                           gggaaagCTCGGCACCAGTGTGCTAACAGGCGGATCGCGCCGTCTGAACCGTGCCCATATCGTTGAACTTGTGCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTACTGCGACGTCGGGCGCGATCGccgccg                                                                                                                            <  2:82646/140‑1 (MQ=255)                              aaaaCTCGGCACCAGTGTGCTAACAGGCGGATCGCGCCGTCTGAACCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGc                                                                                                                   <  1:397604/149‑1 (MQ=255)                               aaaCTCGGCACCAGTGTGCTAACAGGCGGGACGCGCCGTCTGAGCCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGTCGCGATCGCCGCCGGACg                                                                                                                        >  2:508847/1‑143 (MQ=255)                                 acccGGCACCAGTGGGCTACCAGGCGGATCGCGCCGTCTGAACCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCAcc                                                                                                                <  1:684649/146‑1 (MQ=255)                                  cgcGGCACCAGGGGGCTAACAGGCGGGTCGCGCCGTCTGAACCGTGCCCACATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACACGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGa                                                                                                                     <  2:1006476/141‑1 (MQ=255)                                  cTCGGCACCGGGGTGCTAACAGGCGTATCGCGCCGTCTGACCCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGa                                                                                                                     <  2:263695/143‑1 (MQ=255)                                          cAGTGTGCTAACAGGCGGATCGCGCCGTCTGAACCGTGCCCATATCGTTGAACTTGTAAAACACCGCGCGCTGTCACAAACCTCCGGCCATCGGATTGTTATAGTGACGTCGGGCGCGATCGCCGCCGGACGTGGGCACCTGGGTTAcc                                                                                                       >  1:629174/1‑149 (MQ=255)                                            gtgtGCTAACAGGCGGATCGCGCCGTCTGAACCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGt                                                                                                           <  2:833173/143‑1 (MQ=255)                                             tgtgCTAACAGGCGGATCGCGCCGTCTGAACCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGCCGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCgg                                                                                                    <  1:551113/149‑1 (MQ=255)                                             tgtgCTAACAGGCGGATCGCGCCGTCTGAACCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCgg                                                                                                    >  1:899008/1‑149 (MQ=255)                                               tgCTAACAGGCGGATCGCGCCGTCTGAACCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGaa                                                                                                  <  1:1262387/149‑1 (MQ=255)                                                     caggcgcccggcgcCGTCTGAACCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTGATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGaa                                                                                                  <  2:1143401/133‑1 (MQ=255)                                                        gCGGATCGCGCCGTCGGGACCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGCTTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGCGCACCTGGGTGACCCGGAACTg                                                                                               >  2:1112018/1‑143 (MQ=255)                                                         cggctcggtccGTCTGGACCGTGCCGATCTCTTTGAACTTGTTCGCCAGTGCGCGCAGGTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGc                                                                                              <  2:204358/134‑1 (MQ=255)                                                               gcgcCGTCTGAACCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGACCACCTGGGTTACCCGGAACTGCCAgagc                                                                                        >  2:172601/1‑140 (MQ=255)                                                                cgcCGTCTGAACCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGAc                                                                                       >  2:907509/1‑143 (MQ=255)                                                                  ccGTCTGAACCGTACCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGATACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCt                                                                               >  1:931883/1‑149 (MQ=255)                                                                     tCTGAACCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACAGGCCGCCGGGCACCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCg                                                                                  <  2:909259/143‑1 (MQ=255)                                                                        gAACCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCt                                                                               >  2:80765/1‑143 (MQ=255)                                                                         aaCCGTGCCCATATCGTTGAACTTGTTCGCCATTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAGCTGCCAGCGACCATCGccgc                                                                              >  2:397015/1‑141 (MQ=255)                                                                          aCCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGGCGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCg                                                                             <  2:459264/143‑1 (MQ=255)                                                                          aCCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGGCGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCg                                                                             <  2:1042704/143‑1 (MQ=255)                                                                                 ccATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTgg                                                                <  1:178990/149‑1 (MQ=255)                                                                                     ctCGGTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTggcggc                                                            <  1:1298899/148‑1 (MQ=255)                                                                                       cGTTGAACTTGTTCGCCAGTGCGCGCCGTTACAGGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTgg                                                                <  2:61226/143‑1 (MQ=255)                                                                                        gTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGt                                                         >  1:1061447/1‑149 (MQ=255)                                                                                        gTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAAATGCTGGCGGCGGt                                                         >  1:49119/1‑149 (MQ=255)                                                                                            aaCTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGgcggcg                                                           >  2:638032/1‑143 (MQ=255)                                                                                             acgtGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGGCGGGCGCGATCGCCGCCGGAGGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGTAGGGc                                                    <  1:603106/146‑1 (MQ=255)                                                                                              gttGTGCGGCAGTGCGCGCGGTTACCTGCGGCCGGGCATCGGATTGTTATTGTGGCGTCGGGCGCGATCGCCGCCGGACGTGAGCACCGGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGt                                                         <  2:1059608/142‑1 (MQ=255)                                                                                              cTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGTCGGGCa                                                   >  1:1257508/1‑149 (MQ=255)                                                                                              cTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCCCGAAACAACTGCTGGcggcggg                                                         >  2:108275/1‑142 (MQ=255)                                                                                                tGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGTAGGGCaga                                                 <  1:519316/149‑1 (MQ=255)                                                                                                  ttCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGTAggg                                                     >  2:385315/1‑143 (MQ=255)                                                                                                   tCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGTAGGGCAGAGTc                                              <  1:348813/149‑1 (MQ=255)                                                                                                      ccAGTGCGCGCAGTTACATGCCGCCTGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGTAGGGCAGAGTCGAc                                           <  1:794783/149‑1 (MQ=255)                                                                                                      ccAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGTAGGGCaga                                                 >  2:366401/1‑143 (MQ=255)                                                                                                      ccAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGTAGGGCAGAGTCGAc                                           >  1:202534/1‑149 (MQ=255)                                                                                                      ccAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTACGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAGCTGCCAGCGACCAGCGCCTCGAAACAACTGCTGGCGGCGGTAGGGCaga                                                 <  2:355943/143‑1 (MQ=255)                                                                                                       cggggcgcgcAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGTAGGGCagag                                                <  2:116595/139‑1 (MQ=255)                                                                                                          tGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGTAGGGCAGAGTCg                                             >  2:126906/1‑143 (MQ=255)                                                                                                           gcgcgcAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGTAGGGCAGAGTCGACTGAtt                                      <  1:212526/149‑1 (MQ=255)                                                                                                             gcgcAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGCACTGCCAGCGACCATCGCCTCGAAACAACTGCGGGCGGCGGTAGGGCAGAGGCGACTGCTTcg                                    >  1:196872/1‑148 (MQ=255)                                                                                                                 aGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGTAGGGCAGAGTCGACTGAtt                                      >  2:95409/1‑143 (MQ=255)                                                                                                                   ttACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGTAGGGCAGAGGCGGGTGATTCAACtgtg                              >  1:328668/1‑149 (MQ=255)                                                                                                                        tGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGTAGGGCAGAGTCGACTGATTCAACTGTGGGAAc                         <  1:198120/149‑1 (MQ=255)                                                                                                                        tGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACATGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGTAGGGCCGAGGCGACTGATTCAACtgt                               >  2:147679/1‑143 (MQ=255)                                                                                                                          ccgccgGGCATCGGATTGTTATTGTGCCGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGTAGGGCAGAGTCGACTGATTCAACTGTgg                             <  2:782613/143‑1 (MQ=255)                                                                                                                          ccgccgGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGTAGGGCAGAGTCGACTGATTCAACGGTGGGAACAg                       >  1:593076/1‑149 (MQ=255)                                                                                                                                       gtttGTTCGTGGGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGTAGGGCAGAGTCGACTGATTCAACTGTGGGAACAGCTGTTTTCGAttt          <  1:516417/147‑1 (MQ=255)                                                                                                                                       gATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGTAGGGCATAGTCGACTGATTCAACTGTGGGAACAGCTGTTTTCGAttt          <  1:311437/149‑1 (MQ=255)                                                                                                                                         ttGTTATTGTGACGTCGGGCGCGACCGCCGCGGGGCGTGAGCCCCTGGGATACCCGGAACGGCCAGCGACCATCGCCTCTAAACAACTGCTGGCGGCGGTAGGGCAGAGTCGACTGATTCAACTGTGGGAACAGCTGTTTTCg              <  2:655367/143‑1 (MQ=255)                                                                                                                                           gTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCAGGCGGCGGTAGGGCAGAGTCGACTGATTCAACTGTGGGAACAGCTGTTTTCGAt            <  2:187630/143‑1 (MQ=255)                                                                                                                                            ttATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGTAGGGCAGAGTCGACTGATTCAACTGTGGGAACAGCTGTTTTCGATTTATGGc     >  1:334344/1‑149 (MQ=255)                                                                                                                                            ttATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGTAGGGCAGAGTCGACTGATTCAACTGTGGGAACAGCTGTTTTCGATTTATGGc     >  1:556613/1‑149 (MQ=255)                                                                                                                                            ttATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGTAGGGCAGAGTCGACTGATTCAACTGTGGGAACAGCTGTTTTCGATTTATGGc     >  1:686573/1‑149 (MQ=255)                                                                                                                                               ttGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGTAGGGCAGAGTCGACTGATTCAACTGTGGGAACAGCTGTTTTCGATTTATGGCAtt  <  1:460751/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                ATCATGAGTGACAGCCAGACGCTGGTGGTAAAACTCGGCACCAGTGTGCTAACAGGCGGATCGCGCCGTCTGAACCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGTAGGGCAGAGTCGACTGATTCAACTGTGGGAACAGCTGTTTTCGATTTATGGCATT  >  CP009273/256096‑256386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑