Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,718,882 A→G 10.4% M75T (ATG→ACG)  kgtP ← alpha‑ketoglutarate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,718,8820AG10.4% 272.6 / 17.2 111M75T (ATG→ACG) kgtPalpha‑ketoglutarate transporter
Reads supporting (aligned to +/- strand):  ref base A (38/61);  new base G (6/6);  total (44/67)
Fisher's exact test for biased strand distribution p-value = 5.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01

Only 100 of 112 total aligned reads displayed.

GAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGG  >  CP009273/2718736‑2719026
                                                                                                                                                  |                                                                                                                                                
gAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGCTCCGACACACCTCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCCATTGGGCGCATc                                                                                                                                                >  1:758019/1‑149 (MQ=255)
   cccACGTACCTATAGTTTCATAACCTGTGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAgg                                                                                                                                             <  1:121257/149‑1 (MQ=255)
    ccACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGa                                                                                                                                            <  1:989916/149‑1 (MQ=255)
      aCGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCGTCAGGAAt                                                                                                                                          <  1:995637/149‑1 (MQ=255)
        gTACCTAGAGTTTCATAACCTGCGAGGCAGGCGATAACCAGCGATCCGACACACATCATAAACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATcc                                                                                                                                        <  1:251373/149‑1 (MQ=255)
             tctaGTTTCATAACCCGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGATTATCGGCTATGCGGCCAAATAGCCACCCGCCTCTTGAGGGCACCAGGAGTc                                                                                                                                         >  2:383731/3‑143 (MQ=255)
             tataGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCgcag                                                                                                                                   <  1:1219392/149‑1 (MQ=255)
             cataGTTTCATAACCCGGGAGGCAGGAGATAACCAGCGATCCGACACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACTGCCTATTGGGCGCATCAGGAATCCCgcag                                                                                                                                   <  1:915553/148‑1 (MQ=255)
              ataGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGatacc                                                                                                                                        >  2:191162/1‑139 (MQ=255)
               taGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCgcagca                                                                                                                                 >  1:336242/1‑149 (MQ=255)
                 gTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCaaa                                                                                                                               <  1:927033/149‑1 (MQ=255)
                 gTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCaaa                                                                                                                               >  1:88852/1‑149 (MQ=255)
                  tttCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCaaaa                                                                                                                              >  1:1043968/1‑149 (MQ=255)
                       tAACCTGGGAGGCAGGCGATAACCAGCGGTCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCGTCAGGAGTCCTgcggcggg                                                                                                                               >  2:505683/1‑140 (MQ=255)
                       tAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAGCACATCATACACACCGATAACAGCCTCGAGTTTTTGCGACCATGTTTCTCGGCTATGCGGCCAAGTAGCCAACCGCCTATTGGGCGCGTGAGGAATACCgcaagaacaagaac                                                                                                                         >  1:231033/1‑138 (MQ=255)
                       tAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCAACAGGGAACCCACAGCaaa                                                                                                                               >  2:774004/1‑143 (MQ=255)
                        aaCCTGGGAGGCAGGCGATAAACAGCGATCCGAAACCCATCCTACCCACCGATAACTGCATCGATTTTTTGTGACCCTGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCaaaa                                                                                                                              <  2:646/143‑1 (MQ=255)
                          ccTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGGTAAACGCATCGCTTTTTTGCGACCATATTTATCGGCTATGCGGCCAAATAGCCAACCGCCCATTGGGCGCATCGGGAATCCCGCACCAAAAac                                                                                                                            >  2:345193/1‑143 (MQ=255)
                            tGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCGTCAGGAATCCCGCAGCAAAAacac                                                                                                                          <  2:225446/143‑1 (MQ=255)
                               gAGGCAGGCGCTAACCAGCGATCCGAAACACATCATACACACCGATAACAGCTTCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACctg                                                                                                                       <  2:1040019/143‑1 (MQ=255)
                                cgtcaagccatcaCCAGCGATCCGAACCGCCTCATACACACCGATGTCGGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCTAATAGCCAACCGCCGATTGGGCGTATCAGGAATCCCGCAGCAAAAACACctgc                                                                                                                      <  2:617752/131‑1 (MQ=255)
                                cggcagccGATAACCAGCTATCCGAAACACATCAGACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTg                                                                                                                <  1:385258/148‑1 (MQ=255)
                                aggcaggcGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTg                                                                                                                <  1:808947/149‑1 (MQ=255)
                                  gcaggcGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTgta                                                                                                              <  1:1184018/149‑1 (MQ=255)
                                   caggcGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTgtag                                                                                                             >  1:1097098/1‑149 (MQ=255)
                                      gcaaTAACCAGCCATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTg                                                                                                                <  2:467386/140‑1 (MQ=255)
                                       cGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTgt                                                                                                               <  2:226635/143‑1 (MQ=255)
                                       cGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTagtagt                                                                                                         >  1:761513/1‑149 (MQ=255)
                                         aTAACCAGCGATCCGAAACACATCATACACGCCGGTAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTg                                                                                                       >  1:1288015/1‑149 (MQ=255)
                                         aTAACCAGCGATCCGAAACACATCATACACCCCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCTCCTATTGGGCGCATCAGGAATCCCGCCGCAAAAACACCTGCTGTAGGTAGTAGTTg                                                                                                       >  1:529964/1‑149 (MQ=255)
                                          tAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGa                                                                                                      <  1:609934/149‑1 (MQ=255)
                                           aaCCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCCACCGCCTATTGGGCGCGTCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAg                                                                                                     >  1:798916/1‑149 (MQ=255)
                                             ccAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAgtc                                                                                                   <  1:289873/149‑1 (MQ=255)
                                                gccaTCCGAAACACTTCTTACTCACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCTGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGa                                                                                                      <  2:553595/140‑1 (MQ=255)
                                                 cGATCCGAAACACATCATACACACCGATAACAGCCTCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGGGCATCGGGAATCCCGCAGCAAAACCAGCTGCTGTTTGTAGTAGTTGAg                                                                                                     >  2:408921/1‑143 (MQ=255)
                                                   aTCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAgac                                                                                                   >  2:6827/1‑141 (MQ=255)
                                                   aTCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTCATCGGCTATGCGGCCAAATGGCCAACCGCCTATTGGACGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGGCGTAGGTGAgtc                                                                                                   >  2:438244/1‑143 (MQ=255)
                                                      cGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTc                                                                                          >  1:305548/1‑149 (MQ=255)
                                                       gAAACACACCATACACACCTATAACCGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTcc                                                                                         <  1:1246243/149‑1 (MQ=255)
                                                        aaaCCCATCATACACTCCGCTAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTccc                                                                                        <  1:1020475/149‑1 (MQ=255)
                                                             catcatACACACCGATAACAGCATCGATTTTTTGCGACCATGTATAACAGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCGTCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCggaag                                                                                   <  1:746542/149‑1 (MQ=255)
                                                             catcatACACACCGATAACAGCATCGATTATTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCGAACCGCCTATTGGGCGCATCAGGACTCCCGCAGCAAAAACACCCGCTATTTGTAGTAGTTGAGTCGACGTGTTCCCggaac                                                                                   >  1:796310/1‑148 (MQ=255)
                                                                cctACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAAGAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCggaaggga                                                                                <  1:414347/147‑1 (MQ=255)
                                                                catACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCggaaggga                                                                                >  1:307007/1‑149 (MQ=255)
                                                                 atACACACCGATAACAGCATCCATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCggaagggaa                                                                               <  1:451634/149‑1 (MQ=255)
                                                                    cacacCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGgaagaa                                                                            <  1:598005/149‑1 (MQ=255)
                                                                        ccGATAACAGCATCGATTTCTTGCGACCCTGTTTACCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTCGAGTCGTCGTGTTCCCGGAAGGGAAGAAGAtg                                                                        <  1:85951/149‑1 (MQ=255)
                                                                         cGATAACCGCCTCGATTTTTTCGGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTGGAGTCGTCGTGTTCCCGGAAGGgaaga                                                                             <  2:375697/143‑1 (MQ=255)
                                                                           aTACCAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACATCTGCTGTTTGTAGTAGTTAAGTCGGCGTGTTCCCGGAAGGGAAGAAGATGTgg                                                                     <  1:718813/149‑1 (MQ=255)
                                                                           aTAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTgg                                                                     <  1:1203804/149‑1 (MQ=255)
                                                                           aTAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTgg                                                                     <  1:1201547/149‑1 (MQ=255)
                                                                           aTAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCAGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGaagaag                                                                           >  2:259819/1‑143 (MQ=255)
                                                                              aCAGCATCGTTTTTTTGCGACCATTTTTATCGGCTATGCCGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGAtg                                                                        <  2:848030/143‑1 (MQ=255)
                                                                               cAGCCTCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCAAACCGCCGATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTTTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGAtgt                                                                       <  2:656896/143‑1 (MQ=255)
                                                                                 gCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTgg                                                                     <  2:634310/143‑1 (MQ=255)
                                                                                    tCGATTTTTTGCGACCATGTTTAACGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTag                                                            >  1:683715/1‑149 (MQ=255)
                                                                                      ggttttttGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTTTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGCGAACAAGATGTTGGCaaa                                                                <  2:1073635/141‑1 (MQ=255)
                                                                                       aTTTTTTGCCACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGt                                                         <  1:991661/149‑1 (MQ=255)
                                                                                        ttttttGCGACCATGCTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTg                                                        <  1:1190358/149‑1 (MQ=255)
                                                                                          tgttGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGaa                                                      <  1:1065364/147‑1 (MQ=255)
                                                                                           tttGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTaga                                                           <  2:266569/143‑1 (MQ=255)
                                                                                             tGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAg                                                   <  1:1124301/149‑1 (MQ=255)
                                                                                               cGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGaa                                                 <  1:1015128/149‑1 (MQ=255)
                                                                                                gACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGaa                                                      <  2:1112861/143‑1 (MQ=255)
                                                                                                gACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAAc                                                >  1:425159/1‑149 (MQ=255)
                                                                                                gACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGCAAGAAGATGTGGGCAAAGTAGAGTGaa                                                      <  2:1112828/143‑1 (MQ=255)
                                                                                                ccccATGTTTAGCGGTCATTCGCTCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGGTTGTAGTAGTGGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGaa                                                      <  2:793537/141‑1 (MQ=255)
                                                                                                   cATGTTTATCGGGTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAg                                             <  1:399983/149‑1 (MQ=255)
                                                                                                    aTGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCGTAAGGAATCACGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGa                                                  >  2:539980/1‑143 (MQ=255)
                                                                                                    aTGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGt                                            <  1:1102935/149‑1 (MQ=255)
                                                                                                     tcttcaTCGGCTACGCGGCCAAATAGCCAACCGCCTATTGGGCGCGTCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTa                                           <  1:589922/144‑1 (MQ=255)
                                                                                                      gTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGGATCAGGAATCCCTCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAAc                                                <  2:171323/143‑1 (MQ=255)
                                                                                                        ttATCGGCAATGCGGCCAAATTGTCAACCGCCTGTGGGGCCCATCAAGAATCCCGCAGGAAGAACACCGGCTGTTTGTATTAGTCGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAc                                        <  1:498759/149‑1 (MQ=255)
                                                                                                            cGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAg                                    <  1:210014/149‑1 (MQ=255)
                                                                                                              gttATGCGGCCAAATAGCCAACCGCCTCTTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAACATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAc                                        <  2:702802/141‑1 (MQ=255)
                                                                                                              gCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAc                                        >  2:614595/1‑143 (MQ=255)
                                                                                                               cTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGGAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACGTAGaag                                 >  1:575340/1‑148 (MQ=255)
                                                                                                                   gCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGa                             <  1:870851/149‑1 (MQ=255)
                                                                                                                    gggCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGGAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGaa                            <  1:660821/148‑1 (MQ=255)
                                                                                                                     ggacAAACAGCCAACCGCCGATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAAc                           <  1:92477/146‑1 (MQ=255)
                                                                                                                     ggCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGaaa                                 >  2:500776/1‑143 (MQ=255)
                                                                                                                     cgCCCAAGAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTGTGTAGTAGTTGAGTCGTCGGGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGacc                           <  1:92799/148‑3 (MQ=255)
                                                                                                                         aaaTAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAACAACACCGGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGACCGAGTAGACATAGCAATcca                             <  2:709269/143‑3 (MQ=255)
                                                                                                                         aaaTAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGACGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGCAATCGAACCACt                       >  1:67344/1‑149 (MQ=255)
                                                                                                                           gtAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAAc                           <  2:916815/142‑1 (MQ=255)
                                                                                                                              gCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGAcc                  >  1:855107/1‑149 (MQ=255)
                                                                                                                                cAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGACCGAGTAGCCATAGAAATCGAACCACTCAACCAg                >  1:1118170/1‑149 (MQ=255)
                                                                                                                                cAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAg                >  1:67043/1‑149 (MQ=255)
                                                                                                                                cAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAAACGAACCACTCGAACAg                >  1:610683/1‑149 (MQ=255)
                                                                                                                                 aaCCGCCTATTGGGCGCGTCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGa               >  1:1145409/1‑149 (MQ=255)
                                                                                                                                 aaCCGCCTATTGGGCGCGTCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGGGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGa               >  1:512279/1‑149 (MQ=255)
                                                                                                                                   ccGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGGTGAGTCGGCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGAc                   >  2:974372/1‑143 (MQ=255)
                                                                                                                                     gCCTATTGGGCGCGTCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCa                 <  2:752236/143‑1 (MQ=255)
                                                                                                                                     gCCTATCGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCa                 >  2:869680/1‑143 (MQ=255)
                                                                                                                                     gCCTACTGGTCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTAc           <  1:260194/149‑1 (MQ=255)
                                                                                                                                        tATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCTACCCACTCGACCAGAt              <  2:134660/143‑1 (MQ=255)
                                                                                                                                            gggCGCATCAGGAATCCCGCAACAAAAACACCTGCTGATTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGCGAAGAAGATGTTGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACCGAACCAGACTAcc          >  2:306322/1‑143 (MQ=255)
                                                                                                                                             ggCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGAGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAagag   >  1:300623/1‑149 (MQ=255)
                                                                                                                                              gcgcATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGACCCACTCGACCAGATTACCTGAAGAgg  >  1:899684/1‑149 (MQ=255)
                                                                                                                                              gcgcATCAGGAATCCCGCAGCAAAAACACCTGCGGTTTGTAGTAGTTGAGTCGTCGTGTGCCCGGAAGGGGAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTg        >  2:837478/1‑143 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGG  >  CP009273/2718736‑2719026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑