Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	3,204,161	G→A	E8K (GAA→AAA)	rpsU →	30S ribosomal subunit protein S21

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,204,161	0	G	A	100.0%	136.6 / NA	40	E8K (GAA→AAA)	rpsU	30S ribosomal subunit protein S21
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/18);  total (22/18)

CCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGT  >  CP009273/3204017‑3204304                                                                                                                                                 |                                                                                                                                                ccTTTAAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAaaaa                                                                                                                                             <  1:254399/149‑1 (MQ=255)  cTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAAc                                                                                                                                            >  1:202763/1‑149 (MQ=255)  cTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAAc                                                                                                                                            >  1:374106/1‑149 (MQ=255)   tttCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGGAGTAAAATTCCGCACCATTCTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACg                                                                                                                                           >  1:812870/1‑149 (MQ=255)   tttCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACg                                                                                                                                           >  1:758303/1‑149 (MQ=255)       aaaagaGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAACTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAaaaa                                                                                                                                             <  2:653875/143‑1 (MQ=255)           aaGGCCGGGGTGCTTTACAAAGCAGCAGAAATGGCAGTAAAATTCCGCACCAGTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTc                                                                                                                                   <  1:12447/149‑1 (MQ=255)               ccGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACg                                                                                                                               <  1:527204/149‑1 (MQ=255)               ccGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCAGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTAGACg                                                                                                                               >  1:132709/1‑149 (MQ=255)                      gCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGt                                                                                                                              <  2:184286/143‑1 (MQ=255)                      gCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGt                                                                                                                              <  2:335445/143‑1 (MQ=255)                            cAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCg                                                                                                                  >  1:729541/1‑149 (MQ=255)                             aaaGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGGTCTg                                                                                                                       >  2:660148/1‑143 (MQ=255)                              aaGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGc                                                                                                                      <  2:692854/143‑1 (MQ=255)                                    cagcaATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCt                                                                                                                >  2:168259/1‑143 (MQ=255)                                      gcaATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTTGCGTTGATGCCAGCGGCAAACCGAATGAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTc                                                                                                              >  2:736457/1‑143 (MQ=255)                                                tAAAATTCCGCACCATTTTGCAATAAGCTGGCGTTGATGCCAGCGGCCAACCGAATTAATCAAAGGTGAGAGGCACCTGCCGGTAATTAAAGTACGTACAACCGAGCCCTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaaa                                                                                              >  1:870548/1‑149 (MQ=255)                                                tAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaaa                                                                                              <  1:798605/149‑1 (MQ=255)                                                   aaTTCCCCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCg                                                                                                 <  2:630570/143‑1 (MQ=255)                                                      tCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAg                                                                                        >  1:211568/1‑149 (MQ=255)                                                        cGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAACGCAGgt                                                                                      >  1:683581/1‑149 (MQ=255)                                                                     aaTAAGCTGGCGTTGACGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAg                                                                         <  1:278205/149‑1 (MQ=255)                                                                        aaGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACTTGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtc                                                                      <  1:724014/149‑1 (MQ=255)                                                                         aGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcg                                                                     >  1:83695/1‑149 (MQ=255)                                                                            tGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCACGTTACGCGTACCTGCGAAAAAGCAGGTGTTATGGCGGAAGt                                                                        >  2:375521/1‑143 (MQ=255)                                                                              gCGTTGATGCAAGCGGCAAGCCGAATTAATCAAGGGTGAGAGGCACATGCCGGTAATTAAAGTCCCTAAAAACGAGCAGTTCGACGTAGCTCTGCGTCGCTTCAAGCAAAAAAAAAAAAAAGCAGGTGTTCTGGCGGAAGTtc                                                                      <  2:745969/143‑1 (MQ=255)                                                                                  tGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAACACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGa                                                            >  1:579627/1‑149 (MQ=255)                                                                                      ggcAGCGGCAAACCGAATTAATCAAGGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGAAAAAAAAAAAAAGCAGGTGTTCTGGCGGAAGTTcgtcgtcgt                                                              <  2:118300/141‑1 (MQ=255)                                                                                       ccAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCt                                                       >  1:200710/1‑149 (MQ=255)                                                                                       ccAGCGGCAAAACGAATTAATCAAAGGTCAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCt                                                       <  1:4256/149‑1 (MQ=255)                                                                                        cAGCGGCAAACCGAAGTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGa                                                            <  2:512148/143‑1 (MQ=255)                                                                                                  ccGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAACCCg                                            >  1:578865/1‑149 (MQ=255)                                                                                                         aaTCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGa                                           <  2:389084/143‑1 (MQ=255)                                                                                                                      agGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAgcgcgc                        >  1:401936/1‑149 (MQ=255)                                                                                                                        gCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCGCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAgc                            >  2:664977/1‑143 (MQ=255)                                                                                                                             tGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGCGAGTTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCt                 <  1:582148/149‑1 (MQ=255)                                                                                                                                 ggTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTaaaa                   >  2:171973/1‑142 (MQ=255)                                                                                                                                   caaTTAAAGTACGGAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCa           <  1:39586/148‑1 (MQ=255)                                                                                                                                        aaaGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGaa      >  1:782197/1‑149 (MQ=255)                                                                                                                                            tACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGt  >  1:605572/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                CCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGT  >  CP009273/3204017‑3204304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑