Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,176,704:1 +T 6.5% intergenic (+22/+468) zupT → / ← ribB zinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,176,7041.T 6.5% 244.9 / 10.8 62intergenic (+22/+468)zupT/ribBzinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base . (36/22);  new base T (2/2);  total (38/24)
Fisher's exact test for biased strand distribution p-value = 6.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.26e-01

GTTGATGAATTAATGCCGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATGC  >  CP009273/3176557‑3176851
                                                                                                                                                    |                                                                                                                                                   
gTTGAGGAATTAATGCCGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAg                                                                                                                                                    <  2:359162/149‑1 (MQ=255)
    atggATTGATGCCGCTCGACACAGGGCTTGATTCTTACAGTCACCCCAGCTTTGGCGGTCTATGTGGAATTTCTGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCa                                                                                                                                                <  1:387246/149‑1 (MQ=255)
     tgaATTAATGCCGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCAt                                                                                                                                               >  2:44341/1‑149 (MQ=255)
     tgaATTAATGCCGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCAt                                                                                                                                               <  1:504015/149‑1 (MQ=255)
       aaTTAATGCCGCTGGCCAAAGGGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGTATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATgg                                                                                                                                             >  2:615383/1‑149 (MQ=255)
         ttAACGCCGCTCGCCACAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGAc                                                                                                                                           <  2:164696/149‑1 (MQ=255)
           aaTGCCGCTCGCCAAAGAGATTGACCCTAATAATACCCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACaa                                                                                                                                         <  2:842575/149‑1 (MQ=255)
            acgCCGCTCGACAAAGAGATTGACTCTAATAATCACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAAc                                                                                                                                        <  2:723273/147‑1 (MQ=255)
              gCCGCTCGCCAAAGGGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGGGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCAGCAAACGGCGGGAATTGGTTAAAGACAAAATAAGAAGGTGTCCAggacgtggaagactg                                                                                                                                      >  2:9860/1‑135 (MQ=255)
                cGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTGTGTGGAATGTCAGTGATGGGAGTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGCAGTTGTCAAGCGCATGTACaaagtct                                                                                                                                    >  2:228513/1‑144 (MQ=255)
                  cTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAAAGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGc                                                                                                                                  >  1:556438/1‑149 (MQ=255)
                        aaaGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCtt                                                                                                                            >  2:89749/1‑149 (MQ=255)
                          agagATTGACCCTAATAATACCCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACTGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCa                                                                                                                          <  2:338698/149‑1 (MQ=255)
                           gctatggACGCTAATAACACCCCGCGCTATGGCGTACGATGTTGAATGTCAGTGATGGGAGTCAGTTGAGTGCTGCCACAAACGGCGGGAATTGGTTAAAAACAACGTCCGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCAc                                                                                                                         <  1:753770/143‑1 (MQ=255)
                            agaTTGACCCTACTACTCTCCCTAGCTATGGCGTACTATGTGGAAGGTCAGTGATGGGATTCAGTTTAGTTCTGCTACTAACGGCGGGAATTGGTTAAAAACAACAGCAGAAGTTGTCCATGCGCTGGACAACTTCTgatgttgttttt                                                                                                                        <  1:684527/149‑12 (MQ=25)
                            agaTTGACCCTAATGATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACAGCAGAAGTTGTCCATGCGCTGGACAACTTCTgatgttgttttt                                                                                                                        <  1:717541/149‑12 (MQ=37)
                              aTTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCTGCTTCACGaa                                                                                                                      >  2:329187/1‑149 (MQ=255)
                                 gACCTTAATGATAACCCCAGCTAGGGCCTACTATGTGGAATGTCAGTGATGGGAGTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGc                                                                                                                   <  1:146718/149‑1 (MQ=255)
                                    ccTAATAGTAACGCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTTTCCAGCGCATGGACAACTTCTACCAGCATCACGaaacactt                                                                                                                >  1:58630/1‑144 (MQ=255)
                                    ccTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTTCTACACACGGCGCGTATCTGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTACCAGCTTCCCGCAAGCCtt                                                                                                                >  1:742277/1‑149 (MQ=255)
                                    ccTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCtt                                                                                                                >  1:160460/1‑149 (MQ=255)
                                    ccTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGATTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCACCTGCACTAAATCCtt                                                                                                                >  1:564585/1‑149 (MQ=255)
                                           ataaCCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCCGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTCATTCTag                                                                                                         >  1:432028/1‑148 (MQ=255)
                                            taaCCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTAcc                                                                                                        <  2:380205/149‑1 (MQ=255)
                                                      tATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTg                                                                                              >  2:740729/1‑149 (MQ=255)
                                                       aTGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGt                                                                                             <  2:780980/149‑1 (MQ=255)
                                                         gccgtcccgtgttGAATGTCGGTGATGTGATTCAGCTTAGTGGTGCTAGCACCGGAGGGAATTGGTTAAAATCTACATCAGAAGTGGTCCAGCGCATGGACAACTTCTGCCAGCGTCACGAAAGCCTTATTCTACCTTTTCGGCTGtct                                                                                           <  1:422740/140‑1 (MQ=255)
                                                             tACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctcttc                                                                                       >  2:768969/1‑149 (MQ=255)
                                                              aCTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTTTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAATGCCTTATTCTACCTTTTCGGCTGtctcttcc                                                                                      <  1:635359/149‑2 (MQ=255)
                                                              aCTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctcttct                                                                                      <  2:83905/149‑1 (MQ=255)
                                                               cTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctcttctc                                                                                     >  2:537936/1‑149 (MQ=255)
                                                                  tgtgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTAATTCTAACTTTTCGGCTGTCTCTTCTCTCg                                                                                  >  2:583226/1‑149 (MQ=255)
                                                                            cAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTaa                                                                        >  1:130778/1‑149 (MQ=255)
                                                                             aGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAg                                                                       >  2:695025/1‑149 (MQ=255)
                                                                                 aTGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTAtt                                                                   >  2:735032/1‑149 (MQ=255)
                                                                                 aTGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTAtt                                                                   >  2:39092/1‑149 (MQ=255)
                                                                                 aTGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTAtt                                                                   <  1:291755/149‑1 (MQ=255)
                                                                                  tGGGAGTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGTTTAACAACAACATCAGAAGTTGTCCAGCGCATGGACTTCCTATCCTACCGTCACTGGAGCCTTTTTCTACCTTTTCGGCTCTCTCTCCTCTCGTACTGTTTACGTAttt                                                                  <  1:401114/149‑1 (MQ=255)
                                                                                    ggATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTAACTTTTCTGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGt                                                                >  2:377421/1‑149 (MQ=255)
                                                                                    ggAATCAGCTTAGGTCTGCTACAAACGGCGGGAATTGGTTAGAAACAAGATGAGGAGTTGTCCAGAGCATAGACAACTTCTACCAGCCTCACCCAAGCCGTATTCTACATTTTCTGCTGTCTCCTTTCTCGCACTGTTTAAGTCttttt                                                                >  1:61557/1‑147 (MQ=255)
                                                                                       ttCCGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTCAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTCCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTcc                                                             <  2:566920/149‑1 (MQ=255)
                                                                                        tCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcg                                                            >  2:826576/1‑149 (MQ=255)
                                                                                         cAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcgc                                                           <  1:259498/149‑1 (MQ=255)
                                                                                                gTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTACCGCGCTTCaa                                                    >  2:59719/1‑149 (MQ=255)
                                                                                                     gctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGCCAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTAATTGTTCCGCGCTTCAAACAGt                                               >  1:41120/1‑149 (MQ=255)
                                                                                                       tACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGc                                             <  1:810688/149‑1 (MQ=255)
                                                                                                         cAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGt                                           <  2:675023/149‑1 (MQ=255)
                                                                                                          aaaCGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGtc                                          >  2:199769/1‑149 (MQ=255)
                                                                                                           aaCGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGtct                                         <  1:740729/149‑1 (MQ=255)
                                                                                                               gcggGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGt                                     >  2:105929/1‑149 (MQ=255)
                                                                                                                         tttAAAAACAACATCAGAAGGTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGTCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTCAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGaaa                           <  1:195584/148‑1 (MQ=255)
                                                                                                                           tAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGGAACg                         >  1:393939/1‑149 (MQ=255)
                                                                                                                              aaaCAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGGGTCTCTGTCCTGGCGGATCGccc                      >  1:709025/1‑149 (MQ=255)
                                                                                                                               aagaaaaTCAGAGGTTGTCCAGCGTGGGGAGATATGCTGAAAGGTTGAGGGAGGCCTTATTGTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTGTAAGTATTTGTTCTGGGCTTCAAGCAGTGCGTTTCTGTCCTGGCGATACGccgg                     >  1:79718/7‑147 (MQ=255)
                                                                                                                                  gctggcAGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACa                  >  2:404475/6‑149 (MQ=39)
                                                                                                                                      tCAGAGGTTGTCCCGCGCATGGACCACTTCTGCCAGCTTCCCGAATGCCTTCTCCTACCTCTCCGCCTGTCTCTTCTCTCGTACTGTTTACGTATTTGTTCCGCGCTGCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCTCGACAATaa              <  1:651996/149‑1 (MQ=255)
                                                                                                                                       cAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCGTCAAGCAGTGCGTCTCTGTCCTGGCGAGACGCCCGACAGTAAc             >  1:290990/1‑149 (MQ=255)
                                                                                                                                           aGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGGAACGGCCGACACTAACGttt         >  1:607058/1‑149 (MQ=255)
                                                                                                                                           aGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAAGAACGttt         >  2:862783/1‑149 (MQ=255)
                                                                                                                                            gTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGCAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGttta        >  2:316636/1‑148 (MQ=255)
                                                                                                                                                tCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAACCTTATTCCACCCTTTCGGCTGTCTCTCCTCACGTACTGGTTAAGTCGTTGCTCCGCGCATGAAGCAGTCCGTCGCTGTCCTGCCGAAACGCCCGACAATAACGTTGCacag    >  1:733819/1‑146 (MQ=255)
                                                                                                                                                 ccAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGCCAATAACGTTTCAAATg   >  1:73929/1‑149 (MQ=255)
                                                                                                                                                 ccAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATg   >  1:805534/1‑149 (MQ=255)
                                                                                                                                                  cAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATGc  >  2:317253/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GTTGATGAATTAATGCCGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATGC  >  CP009273/3176557‑3176851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: