Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC CP009273 2,400,257 IS5 (+) +4 bp 73.8% intergenic (‑137/‑780) lrhA ← / → alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 2282398 =NA (NA)45 (0.900) 31/276 NT 72.0% noncoding (1195/1195 nt) IS5 repeat region
?CP009273 2400257 = 17 (0.350)intergenic (‑137/‑783) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2
* ? CP009273 = 2283592NA (NA)51 (1.020) 39/276 NT 74.4% noncoding (1/1195 nt) IS5 repeat region
?CP009273 = 2400260 17 (0.350)intergenic (‑140/‑780) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

AAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTT  >  CP009273/2282251‑2282542
                                                                                                                                                   |                                                                                                                                                
aaaTTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATaagg                                                                                                                                                 >  1:282508/1‑149 (MQ=255)
  aTTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCGAATGCTATGGGTGAATATaaggaa                                                                                                                                               <  2:644467/149‑1 (MQ=255)
  aTTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTACTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATaaggaa                                                                                                                                               <  2:772584/149‑1 (MQ=255)
    tctttgtgatGCTGGTCCCCGCTAGGATTGATTTTCGTATTGTTGGCGGTCCCATAACCCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATaaggaagg                                                                                                                                             <  1:156260/141‑1 (MQ=255)
      ttttATATGCTGTTCTCCGCCAGGATTGCTTTTCGTATTATTAGCGGTCCCATAAACCCGCTGGATATCTTTTTCCAGGACAACACCCTCCACTGTGTCCCCATACTCAAGGGATGATGCGAATGCTATTGGCGTATATacggaagggg                                                                                                                                           >  1:818090/1‑147 (MQ=255)
      ttttATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACCACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCACTGCTATGGGAGAAAATAAGGAAGGTg                                                                                                                                           >  1:673488/1‑149 (MQ=255)
                       cGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAAt                                                                                                                          >  2:781036/1‑149 (MQ=255)
                        gCCAGGATTGATTTTCGTATTAGTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGCCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAAtt                                                                                                                         <  2:92147/149‑1 (MQ=255)
                              attgattTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTtctc                                                                                                                   >  1:544089/1‑149 (MQ=255)
                              attgattTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTtctc                                                                                                                   >  1:532788/1‑149 (MQ=255)
                                   ttttCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATGAGGAAGGTGCGAATAAGCGGGGACATTCTTCTCGGGTg                                                                                                              >  1:845728/1‑149 (MQ=255)
                                                   ggTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcatttcatt                                                                                              >  1:764712/1‑149 (MQ=255)
                                                        cATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATttcttc                                                                                         >  1:838483/1‑149 (MQ=255)
                                                         aTAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCa                                                                                        >  1:588186/1‑149 (MQ=255)
                                                              ccAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGttt                                                                                   <  1:603031/149‑1 (MQ=255)
                                                                 gcgggATATCTTTCTCCAGGACNACACGCTCTACTGTCTCACCATTCTTGACAGTGGATGCCAATGCTATGGGTGAATATATGGAAGGTGCGAATCAGCGGGGAAATTCGTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAg                                                                                <  2:494355/146‑1 (MQ=21)
                                                                    ggATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCg                                                                             <  1:24657/149‑1 (MQ=255)
                                                                         tCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAtttt                                                                        <  2:10503/149‑1 (MQ=255)
                                                                                 cAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCg                                                                <  2:282508/149‑1 (MQ=255)
                                                                                  aGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCAATGGGTGAATATAAGGAAGGTGAGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTGATTTCTTCATGTTTGAGCCAATTTTTTCTACCGt                                                               >  2:80787/1‑149 (MQ=25)
                                                                                         ccccATCAACTGTCTCACCATACTCAACAGTTGATGCCGATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGcc                                                        <  1:175769/147‑1 (MQ=255)
                                                                                         caccacCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGcc                                                        <  2:246453/149‑1 (MQ=255)
                                                                                           ccATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGGTTTTTTGTCCCGTAAATGCGtt                                                      >  1:156669/1‑149 (MQ=255)
                                                                                           ccATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCtt                                                      <  2:85147/149‑1 (MQ=255)
                                                                                              tCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGaa                                                   >  2:269244/1‑149 (MQ=255)
                                                                                                aaCTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAGTc                                                 >  2:651274/1‑149 (MQ=255)
                                                                                                aaCTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATc                                                 >  2:680665/1‑149 (MQ=255)
                                                                                                 aCTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCa                                                <  1:681133/149‑1 (MQ=255)
                                                                                                   tGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGc                                              <  2:581880/149‑1 (MQ=255)
                                                                                                     tctcACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGGCt                                            >  1:402337/1‑149 (MQ=255)
                                                                                                       tcACCATACTCAACAGTTGATGCCAATGCTGTGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTACATGCGTTGAAttctacgat                                          >  1:146182/1‑141 (MQ=18)
                                                                                                          ccATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTa                                       >  2:814625/1‑149 (MQ=255)
                                                                                                             tccTCAACCGTTGATGCCCATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGCTTTTTCCACCCGTAAATGCCTTGAATCAGCCTATTTAGAc                                    <  1:188575/147‑1 (MQ=17)
                                                                                                                tCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTCAATGCCTTGAATCAGCCTATTTAGACCGt                                 <  2:532788/149‑1 (MQ=255)
                                                                                                                tCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGAGGAAATTCTTCTCGGCTGACTCAGTCATTTCCTTCCTGCACGCTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGt                                 >  1:480680/1‑149 (MQ=17)
                                                                                                                  aaCAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGCCCGtgt                               >  2:173507/1‑147 (MQ=255)
                                                                                                                      gTTGATGCCAATGCTATGGGTGAATATAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATGTTTTCTCCCGTAAATGCCTTGAATCAGCCCATTTATGCCGtttttac                           >  2:196816/1‑145 (MQ=17)
                                                                                                                           tGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCAt                      <  1:269244/149‑1 (MQ=255)
                                                                                                                                 tgttATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGCCTGACTCAGTCATTTCATTTCTCCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAgg                <  2:673488/146‑1 (MQ=255)
                                                                                                                                    tATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGt             >  1:701971/1‑149 (MQ=39)
                                                                                                                                     gtGGGTGAATATAAGGAAGGTGCGAATGAGCGGGGAATTTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTGGAGCCGATTTTTTCTTCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGtt            <  1:16670/148‑1 (MQ=255)
                                                                                                                                       gggTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTAt          >  2:99444/1‑149 (MQ=37)
                                                                                                                                         gTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATcc        <  2:141353/149‑1 (MQ=35)
                                                                                                                                               ataAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGCTTCTTCGCCATTTAAGCCGTTATCCCCCGtt  >  1:44778/1‑149 (MQ=2)
                                                                                                                                               ataAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCGGACTCAGTCATTTCATTTCTTCATGTTGGAGCCGATTTTTTCTTCCGTAAATGCCTTGAATCAGACTATTTAGACCGTTTCTTCGCCATTTAACGCTGTATACCCAGtt  >  1:758131/1‑149 (MQ=11)
                                                                                                                                                   |                                                                                                                                                
AAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTT  >  CP009273/2282251‑2282542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.