Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,144,365 G→T 34.4% V51F (GTT→TTT)  fabH → 3‑oxoacyl‑[acyl‑carrier‑protein] synthase III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,144,3650GT34.4% 45.4 / 25.1 40V51F (GTT→TTT) fabH3‑oxoacyl‑[acyl‑carrier‑protein] synthase III
Reads supporting (aligned to +/- strand):  ref base G (15/11);  new base T (8/6);  total (23/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGC  >  CP009273/1144228‑1144502
                                                                                                                                         |                                                                                                                                         
ttattGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATg                                                                                                                                <  2:325273/149‑1 (MQ=255)
  attGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACCAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTTGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCTTTTCAACCATggg                                                                                                                              <  2:30069/149‑1 (MQ=255)
   ttGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGc                                                                                                                             <  1:743724/149‑1 (MQ=255)
     ggTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCtt                                                                                                                           >  1:353398/1‑149 (MQ=255)
      gTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCttt                                                                                                                          >  2:766555/1‑149 (MQ=255)
       tACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCTTTTCAACCATGGGCTTTg                                                                                                                         >  2:728995/1‑149 (MQ=255)
       tACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTg                                                                                                                         >  1:265098/1‑149 (MQ=255)
          tGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGCTTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCCTGGGCTTTGAAg                                                                                                                      >  2:245371/1‑149 (MQ=255)
          tGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGAATTGGAAAACATGGTGGACACCACTGACGAGTGGGTTGTCACTCGTACCGGTATCCGCGAACGCCCCATTGCCGCCCCAAACGACACCGCTCCAACCAAGGGCCTTGAAg                                                                                                                      >  1:499584/1‑149 (MQ=255)
              aGCTATCTGCCCGAACAAGTGCGGACAAACGCAGAATTGGAAAAAATGGGGGACACCACTGAGGGCTGGAATGTCACGCGGACCAGTATCCGCGAACGCCACATAGCCGCGCCAAACGACAGCGTTCCAACCCACGGCTTTGAAccggc                                                                                                                  >  1:174059/1‑149 (MQ=255)
                    cTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATAGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAGCGCAACCTTTTCAACCATGGGCTTTGAAGCGGCGGCAcg                                                                                                            >  2:151178/1‑149 (MQ=255)
                            aCAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTg                                                                                                    <  1:19179/149‑1 (MQ=255)
                                 tGCGGACAAACGCCGCTTTGGAAAAAATGGTAGACACCTCTGACGAGTGGATTGTCACTCGTACCAGTATCCGCGACCGCCACCTTGCCGCGCCAAACGAAACCTTTTCAACCAGGGGCTTTGAAGCGGCGACACCCGCAATTGACATg                                                                                               >  1:359520/1‑149 (MQ=255)
                                 tGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGCCGAGTGGATTGTCACTCGTCCCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAAGCATGGGCTTTGAAGCGGCGAAACGCGCAATTGAAATg                                                                                               >  2:790809/1‑149 (MQ=255)
                                 tGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCTTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATg                                                                                               >  1:344628/1‑149 (MQ=255)
                                 tGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGCCACGCGAGATTGAGATg                                                                                               >  2:58084/1‑149 (MQ=255)
                                  gCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACTGGTATCCGCGAACGTCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATgg                                                                                              <  2:113360/149‑1 (MQ=255)
                                  gCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATgg                                                                                              <  2:689110/149‑1 (MQ=255)
                                      aCAAATGCCGATTTGGAAAAAATGGTGGCCACCTCTGACTCGTGGCTTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCTTTTCAACCATCGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCggg                                                                                          <  1:596462/149‑1 (MQ=255)
                                            gCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCTTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTga                                                                                    >  2:460820/1‑149 (MQ=255)
                                             ccGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCTTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTgag                                                                                   <  1:801044/149‑1 (MQ=255)
                                                 tttGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAg                                                                               <  2:651302/149‑1 (MQ=255)
                                                 tttGGAAAAAATGGTGGACACCCCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGTCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAg                                                                               <  2:424033/149‑1 (MQ=255)
                                                    ggAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGAcc                                                                            <  2:355730/149‑1 (MQ=255)
                                                     gAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAGGACCa                                                                           >  1:562765/1‑149 (MQ=255)
                                                     gAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCCCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGCGAAAGACCa                                                                           >  1:411467/1‑149 (MQ=255)
                                                                      ctctGACGAGTGGATTGTCACTCGTACCGGTATACGCGAACGCCACATTGCCGCCCCAAACGAAACCGTCTCAACCAGGGGCTGTGAAGCGGCGACACGCCCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTtg                                                          >  1:820148/1‑149 (MQ=255)
                                                                         tGACGAGTGGATTGTCACTCGGACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCTTTTCAACCATGGTCTTCGAACCGCCGACAAACGATCTCAATACTGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTTGTgg                                                       <  2:854651/149‑1 (MQ=255)
                                                                          gACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGCTGTGGc                                                      >  1:609635/1‑149 (MQ=255)
                                                                          gACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATGGAGAAAGCCCAGATTGGCCTGATCGGTGTGGc                                                      >  1:455952/1‑149 (MQ=255)
                                                                             gAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGAGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTTGTGGCAAc                                                   >  2:344323/1‑149 (MQ=255)
                                                                                     tGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCTTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTTGTGGCAACGACTTCTg                                           >  2:233450/1‑149 (MQ=255)
                                                                                           tCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCTTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTacgc                                     <  1:422617/149‑1 (MQ=255)
                                                                                            cGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTacgca                                    <  2:770058/149‑1 (MQ=255)
                                                                                                 cGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCtt                               >  1:87507/1‑149 (MQ=255)
                                                                                                                 cacaTTGCCTCGCCAAACGAAACCTTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTTGTTGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTg               <  2:581238/149‑1 (MQ=255)
                                                                                                                     ttGCCGCGCCAAACGAAACCTTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAg           >  2:141822/1‑149 (MQ=255)
                                                                                                                        ccGCGCCAAACGAAACCTTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAAATGGCGGACATTAAGAAAGACCAGATTGGCCTGATCGTTGTGGCAACGACGTCTGCTACGCACGCTTTCCCGAGCGCAGCTTCTCCGAtc        >  1:123562/1‑148 (MQ=255)
                                                                                                                            gcCAAACGAAACCGTTGCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCaaa    <  1:169601/149‑1 (MQ=255)
                                                                                                                              cAAACGGAACCGTTTCAACCATGGCCGTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGc  <  1:453839/149‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                         
TTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGC  >  CP009273/1144228‑1144502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑