Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,299,755 A→G 5.9% L160S (TTG→TCG)  mqo ← malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,299,7550AG 5.9% 186.9 / 2.5 68L160S (TTG→TCG) mqomalate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base A (27/35);  new base G (2/2);  total (30/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.31e-01

CCGTAGTTCACATCGGTACCAATTTCCGTACGCGTGGCTGCCACTTTCTGTTGCGGATCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGC  >  CP009273/2299608‑2299903
                                                                                                                                                   |                                                                                                                                                    
ccGTAGTTCACATCGCGACCAATTACCGTACGCGTGGCTGCCACTTTCTGTTTCGGATCGCGCCCTTCCCTCACGAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCATAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCaa                                                                                                                                                     <  1:56728/149‑1 (MQ=255)
     gtgcccaTCGGGACCTATTTCCGTACGCGTGGCTGCCACTTTCTGTTGCGGATCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACgcgg                                                                                                                                                <  1:240451/144‑1 (MQ=255)
      ttCACATCGGTACCAATTTCCGTACGCGTGGCTGCCACTTTCTGTTGCGGATCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACgcggc                                                                                                                                               <  2:770975/149‑1 (MQ=255)
         acaTCGGTACCAATTTCCGTACGCGTGGCTGCCACTTTCTGTTGCGGATCGCGCCCTTCCATCTCTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCGACGCGGCGTa                                                                                                                                            >  2:579333/1‑149 (MQ=255)
         acaTCGGTACCAATTTCCGTACGCGTGGCTGCCACTTTCTGTTGCGGATCGCGCCCTTCCATCTCTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCGACGCGGCGTa                                                                                                                                            >  2:579542/1‑149 (MQ=255)
         acaTCGGTACCAATTTCCGTACGCGTGGCTGCCACTTTCTGTTGCGGATCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGGGAAACCGCGAGCTTTGTTGCAACGCGGCGTa                                                                                                                                            >  2:286439/1‑149 (MQ=255)
         acaTCGGTACCAATTTCCGTACGCGAGGCTACCACTTTCTGTTGCGGATCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAAAGCGGCGTa                                                                                                                                            >  2:449766/1‑149 (MQ=255)
                tACCAATTTCCGTACGCGTGGCTGCCACTTTCTGTTGCGGATCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGgcg                                                                                                                                     >  2:119018/1‑149 (MQ=255)
                tACCAATTTCCGTACGCGTGGCTGCCACTTTCTGTTGCGGATCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGgcg                                                                                                                                     >  1:331025/1‑149 (MQ=255)
                    aacttCCGTACGCGTGGCTGCCACTTTCTGTTGCGGATCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCa                                                                                                                                 <  2:120378/146‑1 (MQ=255)
                     aTTTCCGTACGCGTGGCTGCCACTTTCTGTTGCGGATCGCGCCCTTCCCTCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACGGCGAGCTGTGTTGCAACGCGGCGGAACGGgcgcgccg                                                                                                                                >  2:619480/1‑147 (MQ=255)
                     aTTTCCGTACGCGTGGCTGCCACTTTCTGTTGCGGATCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAg                                                                                                                                <  2:326687/149‑1 (MQ=255)
                           gTACGCGTGGCTGCCACTTTCTGTTGCGGGTCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAAtt                                                                                                                          >  2:529871/1‑149 (MQ=255)
                           gTACGCGTGGCTGCCACTTTCTGTTGCGGCTCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAACtt                                                                                                                          >  2:529876/1‑149 (MQ=255)
                           gTACGCGTGGCTGCCACTTTCTGTTGCGGATCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAAtt                                                                                                                          >  1:33029/1‑149 (MQ=255)
                              cgcgTGGCTGCCACTTTCTGTTGCGGATCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGAc                                                                                                                       <  2:607651/149‑1 (MQ=255)
                                      tGCCACTTTCTGTTGCGGATCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCt                                                                                                               >  1:69385/1‑149 (MQ=255)
                                      cgCCACTTTCTTGTGCGGCTTGCCTCGTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGGAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCt                                                                                                               <  1:41299/148‑1 (MQ=255)
                                            tttCTGTTGCGGATCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGcccc                                                                                                         >  2:133807/1‑149 (MQ=255)
                                              tCTGTTGCGGATCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAg                                                                                                       <  1:230419/149‑1 (MQ=255)
                                                 gTTGCGGATCGCGCCCTTCCATTATTAACGGTGCCCCCTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACa                                                                                                    <  1:522017/149‑1 (MQ=255)
                                                  ttGCGGATCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACaa                                                                                                   <  1:633904/149‑1 (MQ=255)
                                                     cGGCTCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGc                                                                                                <  2:222872/149‑1 (MQ=255)
                                                       gATCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTc                                                                                              <  2:855683/149‑1 (MQ=255)
                                                       gATCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCCATGTTGCAACGCGGCGTAACGGGCGCGCAGGACATTGACGTTATCCACGCCCCAGCCAAAGccc                                                                                              >  1:776586/1‑147 (MQ=255)
                                                       gATCGCGCCCTTCAATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCCCCCCAGACAAAGCTc                                                                                              >  1:321026/1‑149 (MQ=255)
                                                            cgcCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATg                                                                                         <  1:69292/149‑1 (MQ=255)
                                                               ccTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCGTACCGCGAAACAGCGAGCTTTGTTGCAACGGGGCGGAACGCGCCGGAAGGAGTAGCACTTTATCCTCGCCCCAGACAAAGCTCACATGCgg                                                                                      >  1:182180/1‑149 (MQ=255)
                                                                  tCCATCACTAACGGTGCCCATGCTTTGATCTGCGCGTGATCTGCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAAc                                                                                   <  1:793398/149‑1 (MQ=255)
                                                                  tCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAAc                                                                                   <  1:368521/149‑1 (MQ=255)
                                                                         cTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCCACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTg                                                                            >  1:26008/1‑149 (MQ=255)
                                                                          tAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGa                                                                           >  2:143798/1‑149 (MQ=255)
                                                                               gggCCCCCTCTTTGATCTGCGCGTGATCTTCCGAGTAACGCGTACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAAt                                                                      <  1:308315/147‑1 (MQ=255)
                                                                                      ctctTGGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTCGTTGCAACGCGGCGTAACGGGCGCGCAGGAAAGTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAAcgcg                                                               <  1:508723/149‑1 (MQ=255)
                                                                                            gATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGCACGGTAGTGATAAATGAACGCGGAGGAc                                                         >  1:91503/1‑149 (MQ=255)
                                                                                            gATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTAc                                                         >  2:796787/1‑149 (MQ=255)
                                                                                             aTCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACg                                                        >  2:712027/1‑149 (MQ=255)
                                                                                               cTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACgca                                                      <  2:847872/149‑1 (MQ=255)
                                                                                               cTGCGCGTGAGCTTCGGAGTAACGCATACCGCGAACCAGCGAGCTTTGTTGTAACGCGGCGTAACGGGCGCGCAGGCAATTGACATTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACgca                                                      <  1:549150/149‑1 (MQ=255)
                                                                                                    cgTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAGCGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCAcg                                                 >  1:145420/1‑149 (MQ=255)
                                                                                                          cTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCgcgc                                           <  2:100832/149‑1 (MQ=255)
                                                                                                           ttCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCt                                          >  1:736917/1‑149 (MQ=255)
                                                                                                             cAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCa                                        >  1:680999/1‑149 (MQ=255)
                                                                                                               gcgTAACGCCTACCTCGAAACAGCGGGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAAc                                      <  1:86506/147‑1 (MQ=255)
                                                                                                                 gTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCt                                    <  2:224750/149‑1 (MQ=255)
                                                                                                                 gTAACGCAGACCGCGAAACAGCGAGCTGTGTTGCAACGGGGCGTAACGGGCGCGCAGGAAATTGACGTTACCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATGGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCt                                    <  1:669664/149‑1 (MQ=255)
                                                                                                                  tAACGCATACCGCGAAACAGCGAGCTCTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTg                                   <  2:76995/149‑1 (MQ=255)
                                                                                                                    acgactACCGCGAAATAGCGCGCTTTGTGGCAACGCGCCGTAACGGGCGCGCAGGACATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCCCGCCGCGCTCAACCTGGt                                 <  1:665824/144‑1 (MQ=255)
                                                                                                                    acacATACCGCGAAACGGCGAGCTTTGTTGCAACGCGGAGCACCGCGCCGGCGGGAGCGTTACTTTATCCTCGCACCAGACTGATCTCATATGCGCAACGGTATTGATAAATGAACGCGGAGTACGCAGCACCCCGCGCTCAACCTGGt                                 <  1:613311/146‑1 (MQ=255)
                                                                                                                        aTACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTgcgc                             <  2:565573/149‑1 (MQ=255)
                                                                                                                        aTACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATAGGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTgcgc                             >  1:245308/1‑149 (MQ=255)
                                                                                                                           ccGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACCAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCa                          <  1:336303/149‑1 (MQ=255)
                                                                                                                           ccGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGGGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCa                          >  1:525453/1‑149 (MQ=255)
                                                                                                                           ccGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTgcgacaa                          >  2:819811/1‑145 (MQ=255)
                                                                                                                           ccGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCa                          >  1:632867/1‑149 (MQ=255)
                                                                                                                           ccGCGAAACAGCGAGCTTTGTTGCAACGCGGCGGAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGCAAGGTGAAGACAAATTAACACGGAGGACGCAGCACGCCGCGCTCAACATGGTGCGCCCa                          >  1:650749/1‑149 (MQ=255)
                                                                                                                            cgcgAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAg                         >  1:88294/1‑149 (MQ=255)
                                                                                                                                   cAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGc                  >  2:474210/1‑149 (MQ=255)
                                                                                                                                    aGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCg                 <  2:732708/149‑1 (MQ=255)
                                                                                                                                     gcgcgcTTTGTTGCGACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCgg                <  1:579333/149‑1 (MQ=255)
                                                                                                                                     gCGAGCTTTGTTGCGACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCgg                <  1:579542/149‑1 (MQ=255)
                                                                                                                                     gCGAGCTTTGTTGCCACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCgg                <  2:213921/149‑1 (MQ=255)
                                                                                                                                     gCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCgg                <  2:212510/149‑1 (MQ=255)
                                                                                                                                     gCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCgg                <  2:212538/149‑1 (MQ=255)
                                                                                                                                     gCGAGCTTTGGTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCgg                <  2:337379/149‑1 (MQ=255)
                                                                                                                                         gCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGaaa            <  1:343488/149‑1 (MQ=255)
                                                                                                                                          cTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAAt           <  2:764500/149‑1 (MQ=255)
                                                                                                                                             tgttgCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTg        <  2:491294/149‑1 (MQ=255)
                                                                                                                                                  cAACGCGGCGCCACGGGTGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATg   <  2:329843/149‑1 (MQ=255)
                                                                                                                                                   aaCGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGCACGGTAGTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGCAATCTGAACTGc  >  2:338101/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCGTAGTTCACATCGGTACCAATTTCCGTACGCGTGGCTGCCACTTTCTGTTGCGGATCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGC  >  CP009273/2299608‑2299903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑