Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,420,353 A→T 67.3% intergenic (‑86/+135) hisJ ← / ← argT histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,420,3530AT67.3% 81.7 / 55.0 75intergenic (‑86/+135)hisJ/argThistidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (13/11);  new base T (26/23);  total (39/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

AACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGA  >  CP009273/2420206‑2420501
                                                                                                                                                   |                                                                                                                                                     
aaCGCCGCAGTTGCGCTGGCGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCaaaa                                                                                                                                                      <  2:796226/149‑1 (MQ=255)
   gccgcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTTTTTTTGTTTTCAACTGACAGTCTTTTTATAGGGATTATTTGGTGGTTTGTTACACTTATCGTGCCATTAAATTGTCCATCAAGGCAGAatat                                                                                                                                                   >  1:666439/1‑149 (MQ=255)
   gccgcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAatat                                                                                                                                                   >  2:621479/1‑149 (MQ=255)
       cAGTTGCGCTGGAGCAGGCGAGAACCAGAGAGAGCGATAGAACCAGTTTTTTTCTAAGGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCg                                                                                                                                               <  2:197567/149‑1 (MQ=255)
             cgcTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGGCAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGt                                                                                                                                         >  2:581321/1‑149 (MQ=255)
             cgcTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTTCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGt                                                                                                                                         >  2:67815/1‑149 (MQ=255)
              gcTGGAGAAGACCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGtt                                                                                                                                        >  2:283210/1‑149 (MQ=255)
                tGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAg                                                                                                                                      <  2:776136/149‑1 (MQ=255)
                  gagaAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACATATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGAc                                                                                                                                    <  1:361946/149‑1 (MQ=255)
                        gCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGtatta                                                                                                                              >  2:575983/1‑149 (MQ=255)
                        gCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGtatta                                                                                                                              >  2:353909/1‑149 (MQ=255)
                         ccAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATATTGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTATTACACTTATCTTGCCAGAACATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGttttat                                                                                                                             >  1:526472/1‑149 (MQ=255)
                          cAGAACCAGAGAGAGCGATAGCACCAGTTTTTTTTTTTTTTTTTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATTGTGCCTTTAAATTGACCATCAAGGAAATAAATCCAGCACCGTTAGActttttttt                                                                                                                            >  1:134789/1‑141 (MQ=255)
                          cAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTCTCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGTCGTattatt                                                                                                                            >  1:691219/1‑149 (MQ=255)
                            gAACCAGTGAGAGCGATACCACCAGTTTTTTCATTATGTCCTCCACTGACAGTCTTTTTATAGGGAAGATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTAtttt                                                                                                                          <  2:54387/149‑1 (MQ=255)
                            gAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTAtttt                                                                                                                          <  1:323125/149‑1 (MQ=255)
                                cAGAGAGAGCGATAGCACCAGTTTTTTTTTTTTTTTTTCAACAGAAAGCCGCCTTAGAGAGAATATTAACAGGCTTGATACACTAATCGTGCCATAACATTGACCACCAAGGCAATATAACCCGCCCCGTTAGACGTATTATTTTCCAt                                                                                                                      >  1:861970/1‑149 (MQ=255)
                                   agagagCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTaa                                                                                                                  >  2:326087/1‑149 (MQ=255)
                                       agCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAAt                                                                                                              <  2:777783/149‑1 (MQ=255)
                                       agCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTGATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTCGACGTATTATTTTTCATTAAAAAt                                                                                                              <  2:777728/149‑1 (MQ=255)
                                       agCGATAGCACCAGTTTTTTCAATATGTCCTCAACTGACAGTTTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAAt                                                                                                              <  2:500840/149‑1 (MQ=255)
                                         cGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGc                                                                                                            <  1:98522/149‑1 (MQ=255)
                                           aTAGCACCAGTTTTTTCATAATGTCCTCAACTGACATTCTTTTTATAGGGAATTTTTACAGGTTTGTTACACTTATCGTGACATAAAATTGGCCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCtt                                                                                                          <  2:376843/149‑1 (MQ=255)
                                               cACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCTTGCACTAAAATTGACCATCAAGCCAAAATATCCCGCCCCGTTAGACGTCTAATTTTACATTAAAAATGCTTAAGt                                                                                                      >  2:445538/1‑149 (MQ=255)
                                               cACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTGTTATTTTTCATTAAAAATGCTTAAgg                                                                                                      >  2:445430/1‑148 (MQ=255)
                                                     ttttttCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGACATAAAATTGACCATCAAGGCAATATATCCCGCCCCCTTAGACGTATTATTTTTCATTAAAAAGACTTAAGTaggaat                                                                                                >  1:344200/1‑144 (MQ=255)
                                                      tttttCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGGCGGATTATTTTTCATTAAATATGCTTAAGTAGGCAtt                                                                                               >  2:272828/1‑149 (MQ=255)
                                                            aTAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATg                                                                                         >  1:289700/1‑149 (MQ=255)
                                                            aTAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGGTTGGTACACGTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATg                                                                                         >  2:817739/1‑149 (MQ=255)
                                                                 gTCCTCAACTTACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTCCATTTACATTTTTTAAGTATGCATTGAAATGcatac                                                                                    <  1:405608/149‑4 (MQ=255)
                                                                     tCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGAt                                                                                >  1:674957/1‑149 (MQ=255)
                                                                     tCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGAt                                                                                >  1:474288/1‑149 (MQ=255)
                                                                     tCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGAt                                                                                >  1:19800/1‑149 (MQ=255)
                                                                      cAAATGACAGTCTTTTTATAGGGAATATTTCAAAGTTTTGTACAATTATCCTTCCATAAAAATGGCCATCAAGGCAATAAATTCCGCCCCGGTAGACCTAATAATTTTCATTAAAAAATGCTTAAGTATGCATTTCAATGCACCACGAt                                                                                <  2:152065/149‑1 (MQ=255)
                                                                        aCTGACAGTCTTTTTATAGGGAATATTTACATGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGcaccaggggagt                                                                             <  1:149766/149‑8 (MQ=255)
                                                                            aCAGTCTTTTTATAGGGTATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCTCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGcaa                                                                         <  2:260795/149‑1 (MQ=255)
                                                                            aCAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGcaa                                                                         <  2:688744/149‑1 (MQ=255)
                                                                            aCAGTCTTTTTAGAGGGAATTTTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGcaa                                                                         <  2:617392/149‑1 (MQ=255)
                                                                            aCAGTCTTTTTAGAGGGAATATTTACAGGTCTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATACACCCCGCCACCCTAGACGTATTATTTTAAAAAAAAAATGCTTAAGCATATATTTCAATTCACCACGATAGTGcaa                                                                         <  1:726290/149‑1 (MQ=255)
                                                                            aCAGGCTTTTTTTAGGTAGTTTTTACAGGTTTGTTACACTTAGCGTGCAATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTGCATTATAAATGCTTCAGTATGCATTTCAATGCACCACGATAGTGcaa                                                                         <  1:271916/149‑1 (MQ=255)
                                                                               gTCTTGTCAGAGGCAATATTTACAGGTTTGTTACACTTATCGTTCCAAAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGcaacaa                                                                      <  2:134789/149‑1 (MQ=255)
                                                                                tCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACaaa                                                                     >  2:427978/1‑149 (MQ=255)
                                                                                 cTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCCCGATAGTGCAATAAAc                                                                    >  2:283665/1‑149 (MQ=255)
                                                                                  tttttATAGGGAATATTTACAGGTTTGTAACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAAcc                                                                   >  2:524877/1‑149 (MQ=255)
                                                                                      gctaggtaactattACCGGTTTCTCACCGTTATCGTCCCTCAAAGTTGTCCCGCAATGCAACATATCCCGCCCCGTTCGACGTATTATTTTTCATTAAAAACGCTTAAGTATGCATTTCTATGCTCGACGCTAGTGCAACTAGCCTGGc                                                               <  1:491625/137‑1 (MQ=255)
                                                                                           ggAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCa                                                          >  2:843852/1‑149 (MQ=255)
                                                                                           ggAATATTTACAGGTTTGTTACACTTATCGTGACCTGCAGAGAACCGTCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATCGTGCAACAAACCTGGCGTGCa                                                          >  2:424495/1‑149 (MQ=255)
                                                                                            gtatatTTACATGTTTGTTAAACTTATCGTGCCATAAAATTGCCCATCAAGGCAATATATCCCGCCCCTTTAGAAGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCAc                                                         <  1:384721/147‑1 (MQ=255)
                                                                                               tatTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCat                                                      <  1:283210/149‑1 (MQ=255)
                                                                                               tatTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACATATTATTTAGTTTTATAAAGTCTGTAGTTTGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCat                                                      >  1:526622/1‑149 (MQ=255)
                                                                                                atTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCACCAAACCTGGCGTGCACCatg                                                     >  2:178689/1‑149 (MQ=255)
                                                                                                    cccggTTTGTTCCACTTACCGGGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCAtgatga                                                 <  1:155316/146‑1 (MQ=255)
                                                                                                    caagttctGTTACACTTACCGGGCCATAAAAGTGACCACCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCAtgatga                                                 <  2:419858/142‑1 (MQ=255)
                                                                                                          tttttACGCTTATCGTGCCATAAAATTGACCATCAAGGCAATTTATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACATACCTGGCGTGCACCATGATGATCATTc                                           <  2:240750/146‑1 (MQ=255)
                                                                                                             ttACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCAt                                        <  2:801951/149‑1 (MQ=255)
                                                                                                               acacTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCCCGATAGTGCAACAAACCTGGCGTGCACCCTGCTGGTCATTCCATCa                                      >  1:63953/1‑149 (MQ=255)
                                                                                                                 acTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAgg                                    <  2:111546/149‑1 (MQ=255)
                                                                                                                        gTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCt                             >  2:737932/1‑149 (MQ=255)
                                                                                                                           ccATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTGATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTcc                          <  1:508008/149‑1 (MQ=255)
                                                                                                                           ccATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTcc                          <  1:251978/149‑1 (MQ=255)
                                                                                                                           ccATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTcc                          >  1:153610/1‑149 (MQ=255)
                                                                                                                           ccAAAACATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGCATGCATTTCAATGCACCACGATAGTGCAACAAACCCGGTGTGCACCATGATGTTCACTCCATCAGGTACAGCTTcc                          <  1:795325/149‑1 (MQ=255)
                                                                                                                              tAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCATGCACCATGCTGATCATTCCATCAGGTACCGCTTccccg                       >  2:194233/1‑147 (MQ=255)
                                                                                                                              tAAAATTGACCATCAAGGCAATATATCCCGCCCCGATAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAg                       >  1:541410/1‑149 (MQ=255)
                                                                                                                                 aaTTGACCATCAAGGCAATATATCCCGCCCCGTTCGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAGTGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGTTCATTCCATCAGGTAAAGCTTCCCAGCGa                    >  2:558159/1‑149 (MQ=255)
                                                                                                                                 aaTTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGa                    >  2:241989/1‑149 (MQ=255)
                                                                                                                                  aTTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGAc                   <  1:453260/149‑1 (MQ=255)
                                                                                                                                   ttGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACg                  >  2:576988/1‑149 (MQ=255)
                                                                                                                                      aCCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTAt               <  1:850978/149‑1 (MQ=255)
                                                                                                                                        caacAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCa             <  2:278154/146‑1 (MQ=255)
                                                                                                                                        cATCCAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTCCCTCAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCCACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCa             <  2:69321/149‑1 (MQ=255)
                                                                                                                                        cATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAACATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATAATGATCAGTAAACCAGGCACAGCTTCCCAGCGACgcacca             >  1:397453/1‑144 (MQ=255)
                                                                                                                                        cATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCa             <  1:671945/149‑1 (MQ=255)
                                                                                                                                         aTCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCGCAGCGACGTATCAg            >  2:444635/1‑149 (MQ=255)
                                                                                                                                          tCAAGTCAATATCTCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCTGCGACGTATCAGt           <  1:242739/149‑1 (MQ=255)
                                                                                                                                                cAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCTATCAGGTACAGCTTCCCCGCGACGTATAAGTCCCCGt     >  2:10140/1‑149 (MQ=255)
                                                                                                                                                   aaTACCCCCCCCCGTTACACATATTATTTTGCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGa  <  1:353909/149‑1 (MQ=255)
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AACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGA  >  CP009273/2420206‑2420501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: