Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,421,172 A→C 32.0% D33E (GAT→GAG argT ← lysine/arginine/ornithine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,421,1720AC32.0% 69.3 / 49.8 79D33E (GAT→GAGargTlysine/arginine/ornithine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (30/22);  new base C (16/10);  total (46/33)
Fisher's exact test for biased strand distribution p-value = 8.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.30e-01

CTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTG  >  CP009273/2421024‑2421318
                                                                                                                                                    |                                                                                                                                                   
cTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTa                                                                                                                                                     >  2:303655/1‑149 (MQ=255)
 tttCAGTGAGGGGGTCAGCGCGTCAAAGTCACTGGCAACCCGGGTACATTTCTCCTGCATCCGTTTGCACATCTCCCTACCGAGTTCGATATCAAAGCCAACAAAAGCACCTTGAGCATCTTTCGATGAGAACTGTGCGTAGGTGGTAt                                                                                                                                                    <  1:220114/149‑1 (MQ=255)
 tttCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCTTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCTTCTTTCGATGAGAACGGTGCGTAGGTGGTAt                                                                                                                                                    >  2:584646/1‑149 (MQ=255)
 tttCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCACCAAAATCACCTGTCGCATCTTTCGATGAGAACGGTGCGTCGGTGGTAt                                                                                                                                                    >  1:260855/1‑149 (MQ=255)
 gttCAGTGAGGGGACCAGCGCGCCAAAGTCACGGGCAACCCAGGTACATTTCCCCTGCATCCGTGTTCACATCTCGTTACGGTGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTAt                                                                                                                                                    <  1:51363/148‑1 (MQ=255)
          gtgtgtCAGCGCGTCAAAGTCACTGGCAACCCAGGGACATTTCACCTCCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGa                                                                                                                                           <  1:430381/144‑1 (MQ=255)
           ggtaTCAGCCCGTCAAGGTCACTGGCAACCCGGTTACATGCCCCCTGTATACGTTCGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAGTCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGCTGGTCTCGGTTCCGAt                                                                                                                                          <  1:612908/146‑1 (MQ=255)
             gATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTACtatag                                                                                                                                        >  2:157380/1‑148 (MQ=255)
              aTCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACg                                                                                                                                       >  2:505164/1‑149 (MQ=255)
              aTCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACg                                                                                                                                       >  2:730695/1‑149 (MQ=255)
              aTCACCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCCTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGAACCGATAcc                                                                                                                                       <  2:214731/149‑2 (MQ=255)
                cAGCGCGGCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAGCGGTGCGTAGGTGGGATCGGTTCCGATACGTa                                                                                                                                     <  1:622921/149‑1 (MQ=255)
                 aGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTAc                                                                                                                                    >  1:649270/1‑149 (MQ=255)
                 aGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATACGTTTGCACATCTCGTTACCGGGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGGGCGTAGGTGGTATCGGTTCCGATACGTAc                                                                                                                                    >  1:789893/1‑149 (MQ=255)
                  gcgcGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTAcc                                                                                                                                   >  1:810299/1‑149 (MQ=255)
                   cgcgTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGGTCCGGTACCTACCg                                                                                                                                  >  2:441647/1‑149 (MQ=255)
                         aaaGTCACTGGCAACCCCGGTACATTGCACCTGCATCCGTTTGCACATCTCGTTACCGACATCGATATCAAAGCCAACAAAATCCCCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCg                                                                                                                            <  2:29198/149‑1 (MQ=255)
                            gtgccttgCAACCCCGGTCCATTTCACCTGCATCCGTTTGCACATCTCGCTACCGAGATCGATATCAACGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGTTGCGTAGGTGGTATCCGTTCCGATACGTACCGTCTCCGGTa                                                                                                                         <  2:328447/142‑1 (MQ=255)
                            gTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTa                                                                                                                         >  1:849110/1‑149 (MQ=255)
                                 tGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGcgcc                                                                                                                    >  1:731471/1‑149 (MQ=255)
                                  ggCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGcgccg                                                                                                                   <  2:838163/149‑1 (MQ=255)
                                   gCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGcgccgc                                                                                                                  >  2:729218/1‑149 (MQ=255)
                                       cccAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGGTACGTACCGTCTCCGGTAGCGCCCCATAg                                                                                                              >  1:127318/1‑149 (MQ=255)
                                       cccAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTGGCGCCGCATAg                                                                                                              >  1:401816/1‑149 (MQ=255)
                                       cccAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGAGCGTAGGTGGTATCGGGTCCGATACATACCGTCCCCGGTAGCGCCCCATAg                                                                                                              >  1:27446/1‑149 (MQ=255)
                                        ccAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAAAACGGTGAGTAGGTGGGCTCGGTTCCGATAAGTAACGTCGCGGCTAGCGCCGGATAGc                                                                                                             >  1:24541/1‑149 (MQ=255)
                                        ccAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCAATATCAAGGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTCGCGTCTCCGGTAGCGCCGCATaca                                                                                                             >  1:546604/1‑147 (MQ=255)
                                          aGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTg                                                                                                           <  1:844208/149‑1 (MQ=255)
                                          aGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGACATCAAAGCCAACCAAGACGCCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTTGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTg                                                                                                           <  1:855268/149‑1 (MQ=255)
                                          aGGTACATTTCACCTGCATCCGTTTGCACACCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTg                                                                                                           <  1:845255/149‑1 (MQ=255)
                                            gTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGa                                                                                                         >  2:30622/1‑149 (MQ=255)
                                            gTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGa                                                                                                         >  2:415579/1‑149 (MQ=255)
                                            gTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGCCTCCGGGAGCGCCGCATAGCCGGa                                                                                                         >  1:715304/1‑149 (MQ=255)
                                                     aCCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTGGGTGGTATCGGTTCCGATACGTACCGTCTCCCGTAGCGACGCAGAGCTGGAAGCCGCttt                                                                                                >  1:296866/1‑147 (MQ=255)
                                                      ccTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTGTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCtgtg                                                                                               <  2:56665/149‑1 (MQ=255)
                                                        tGCATCCTTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGGGCGTAGGTGGGATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGga                                                                                             <  2:99150/149‑1 (MQ=255)
                                                            tCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGgagaga                                                                                         >  2:186262/1‑149 (MQ=255)
                                                             ccGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGAc                                                                                        >  2:517499/1‑149 (MQ=255)
                                                               gTTTGCACATTTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAAAGGTGCGTAGGTGGTCTCGGTTCCGATACGTACAGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCg                                                                                      >  1:370644/1‑149 (MQ=255)
                                                                   atcgaTCTCGGTAACGAGATCGATATCAAAGCCAGCAAAATCACCTTTAGCAGCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGCAGCCGCTGTGGAGAGACCGACtc                                                                                  >  1:98114/5‑148 (MQ=255)
                                                                     acaTCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCCTAGCTGGAAGCCGCTGTGGAGAGAGCGACTAAc                                                                                >  2:279437/1‑149 (MQ=255)
                                                                           cGTTACCGAGATCGATATCAAAGCCAACAGAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGAc                                                                          <  2:685339/149‑1 (MQ=255)
                                                                                 cGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTGCGGTGGAGCCGCTTAGCTGGGAGCCGCTGTTGAACGACCGACTACGAAAAACAAAGCg                                                                   >  1:163175/1‑149 (MQ=255)
                                                                                 cGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCCTAGCTGGAAGCCGCTGTGCAGAGACCGACTAACAAAGaaaaacag                                                                    >  1:163730/1‑142 (MQ=255)
                                                                                 cGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCg                                                                   >  2:105991/1‑149 (MQ=255)
                                                                                 cGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCg                                                                   >  2:129376/1‑149 (MQ=255)
                                                                                 cGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCg                                                                   >  2:144201/1‑149 (MQ=255)
                                                                                  gagaTCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCga                                                                  >  1:144532/1‑149 (MQ=255)
                                                                                     aacGATATCAAAGCCAACAAAATCGCCTTTAGCATCGTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGaa                                                               <  1:677764/147‑1 (MQ=255)
                                                                                      tCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGAGGAGAACGGTGCGTAGGTGGTCTCGGTTCCAATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAAt                                                              >  2:624468/1‑149 (MQ=255)
                                                                                        gATCTCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACAGTGCGTAGGTGGTATCGCTTCCGATACGTACCGTCTCCGGGAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAAACg                                                            >  2:131848/1‑149 (MQ=255)
                                                                                         atatCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGGCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGa                                                           <  2:184360/149‑1 (MQ=255)
                                                                                         agaacAAAGCCACAAAAACCACCGTTGTCATCTGTCGATGGGAACGGCGCGTAGGTCGTATCGGTGCCGATACGGACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACGAACAAAGACAGAGCGAGAATCGa                                                           <  1:201693/145‑1 (MQ=255)
                                                                                          tatCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGAc                                                          <  2:370644/149‑1 (MQ=255)
                                                                                          tatCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGGGAATCGAc                                                          >  2:792709/1‑149 (MQ=255)
                                                                                               aaGCCAACAAAATCACCTTTTGCATCTTTCGATGAGAACGGTGCGTAGGTTGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGActtctt                                                     <  1:611459/149‑1 (MQ=255)
                                                                                               aaGCCAACAAAATCACCTTTGGCATCTTTCGATGAGAACGGTGCCTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGActtctt                                                     <  1:460587/149‑1 (MQ=255)
                                                                                               aaGCCAACAAAATCACCTTTAGCATCCTTCGATGACAACGGTGCGGAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGActtctt                                                     <  1:452814/149‑1 (MQ=255)
                                                                                                  ccgacaaAATCACCTTCAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcat                                                  <  1:584646/146‑1 (MQ=255)
                                                                                                  ccAACAAAATCACCTTTAGCATCGTTCAATAAGCGAAGTGCGTAGGGGGTATCGGTTCCGATACGTACCGACGCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGGGACCGACTAACAAAGACGGGACGGAGAACGACTTCTTcat                                                  >  2:210866/1‑149 (MQ=255)
                                                                                                   caacaaAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAACATCGCCTTCTTcgta                                                 >  1:60286/1‑149 (MQ=255)
                                                                                                    aacaaAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGTATGCTTCTTTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcatac                                                >  2:417070/1‑149 (MQ=255)
                                                                                                    aacaaAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGACGTGGCTGATAAGAAACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcatac                                                >  2:199142/1‑149 (MQ=255)
                                                                                                    aacaaAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcatac                                                >  2:819235/1‑149 (MQ=255)
                                                                                                         caTCACGTTTTGCATCTTTCGATGAGAACGGTGCGTAGGTGGTACCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAAc                                           <  2:169219/148‑1 (MQ=255)
                                                                                                          aTCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAAcc                                          <  2:765951/149‑1 (MQ=255)
                                                                                                                tttAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAg                                    >  2:277058/1‑149 (MQ=255)
                                                                                                                   gggctCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGa                                 <  1:759183/145‑1 (MQ=255)
                                                                                                                    gCATCTTTCGAGGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATCCGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGaa                                <  2:91697/149‑1 (MQ=255)
                                                                                                                          ttCGATGAGAACGGGGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGc                          <  1:303655/149‑1 (MQ=255)
                                                                                                                             gATGGGACCGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCttt                       <  1:451022/149‑1 (MQ=255)
                                                                                                                                   aaCGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTGTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTAtg                 >  1:187739/1‑149 (MQ=255)
                                                                                                                                   aaCGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGACAATGGATTTTTTAtg                 >  2:828253/1‑149 (MQ=255)
                                                                                                                                          cGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGGATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTa          >  2:501970/1‑149 (MQ=255)
                                                                                                                                            tAGGTGGTACCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGCAGAGACCGACTAACAAAGACAGAGCGAGAATCGTCTTCTTTATGCTTAACCCTCAAGTGAAAATCGCTTTTTTATGTTGTGTAtt        <  1:657993/149‑1 (MQ=255)
                                                                                                                                               gtggtATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTAttgtg     <  2:731471/149‑1 (MQ=255)
                                                                                                                                               gcggtCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCCCAAGTGAAAATGGCTTTTTTATGTTGTGTAttgtg     <  2:401011/147‑1 (MQ=255)
                                                                                                                                                 ggtCTCGGTCCCGAGACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGTGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTAttgtgtt   <  2:581646/149‑1 (MQ=255)
                                                                                                                                                  gtATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCGTCAAGTGAACATGGCTTTTTTATATTGGTGCttgtgttg  >  1:861579/1‑149 (MQ=255)
                                                                                                                                                  gtATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTAttgtgttg  >  1:105167/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTG  >  CP009273/2421024‑2421318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: