Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,719,426 T→C 38.8% intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,719,4260TC38.8% 51.3 / 79.6 89intergenic (‑321/+2)kgtP/rrfGalpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
Reads supporting (aligned to +/- strand):  ref base T (25/29);  new base C (22/12);  total (47/42)
Fisher's exact test for biased strand distribution p-value = 1.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.67e-01

ACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACCG  >  CP009273/2719280‑2719567
                                                                                                                                                  |                                                                                                                                             
ccGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTAGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAAGGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGa                                                                                                                                             <  1:576018/148‑1 (MQ=255)
   tATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCACCCGTCAGCATGACCTTTCGCACAATTCGacac                                                                                                                                          >  1:541498/1‑146 (MQ=37)
   tATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCACCAATGCAAAAAGGCCGTCCTGCAGGATGGCCTTTCGCATAATTTGTTGc                                                                                                                                          >  2:14730/1‑149 (MQ=255)
    aTGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGTCCTGTCGCCTGATTCCATGcc                                                                                                                                         >  1:170262/1‑149 (MQ=37)
    aTGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGcc                                                                                                                                         >  1:719114/1‑149 (MQ=255)
      ggAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTCGCATATGTGGGAGGATAAGACACGCAGAGTCGCATCAGGCATTTTGTTCTGCGCTACGGCAGAGAGACCCTCCGTCAGGATGGGCCTTCGCATAATATGATGCCTg                                                                                                                                       >  1:74977/1‑149 (MQ=255)
       gaggTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAACAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTgg                                                                                                                                      <  2:856917/146‑1 (MQ=255)
              gCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTcc                                                                                                                               <  1:363597/149‑1 (MQ=255)
               cAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTccc                                                                                                                              >  1:222838/1‑149 (MQ=255)
                aaGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCGTATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCAAACCGAAAAGCATGCGTTTCGCATAATATGATGCCTGGCAGTTCCCt                                                                                                                             >  2:20544/1‑149 (MQ=25)
                aaGTTGGGGAAGCCGTATCCGTCGCTGAATCTGGCATATGTGGGAGTATAAGACGCGTAGCGTCGCATCAGGCATTTTTCTCGGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGGTGCCTGGCAGTTCCCt                                                                                                                             <  2:550485/149‑1 (MQ=37)
                aaGTGGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCt                                                                                                                             <  2:282450/149‑1 (MQ=255)
                   ttGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTAct                                                                                                                          >  1:631265/1‑149 (MQ=255)
                   ttGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTAct                                                                                                                          >  2:551641/1‑149 (MQ=255)
                       ggAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGGCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGGTCCCAACTCTCg                                                                                                                      >  2:135984/1‑149 (MQ=255)
                        gAAGCCGTATCCGTTGCTGAATCTGGCATAGGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGc                                                                                                                     <  2:142352/149‑1 (MQ=255)
                          aggcGTATCCGTTGCTTGATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCCGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCAt                                                                                                                   <  1:240621/146‑1 (MQ=255)
                          aggagTATGCGTTGCTGAATGTGGACGATGCTGGAGTATAAGACGGGCCGCGGCGCACCAGGCCTTTTTTTCTGCGCCAATGCAAAAAGGCCTTCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCAt                                                                                                                   <  1:232951/145‑1 (MQ=18)
                            ccGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATgg                                                                                                                 >  1:774626/1‑149 (MQ=255)
                              gTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCCCCAGCATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATgggg                                                                                                               <  1:438641/149‑1 (MQ=255)
                               tATCCGTTGCTGAATCTGGCATATGGGGGAGTATAAGACGCGCTGCGTCTCATCAGGCATTATTTGCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTAGCAGTTCCCCACTCgcgcggtggga                                                                                                              >  2:174829/1‑142 (MQ=21)
                                aTCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGgag                                                                                                             >  2:795509/1‑149 (MQ=255)
                                    gTTTCTGGATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGCTGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAccc                                                                                                         <  1:302391/149‑1 (MQ=255)
                                      tgccgtctCTGGCGGATGTGGGAGTATAAGACTCGGAGCGTCGCATTAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCCTCAGGATGGCTTTTCGCATAATTTGATGCCTGGCAGTTCCCTCCTCTCGCATGGGGAGACCCca                                                                                                       <  1:141528/142‑1 (MQ=21)
                                      tGCTGAATCTGGCATATGGGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCca                                                                                                       <  2:360158/149‑1 (MQ=255)
                                         tGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCacac                                                                                                    >  1:849855/1‑149 (MQ=255)
                                          gAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCAAATGCAAAAAGGACATCCGTCAGGATGGCCCTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCCCGCATGGCGAGGCcacacagc                                                                                                   >  1:498975/1‑147 (MQ=255)
                                          gAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGAATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATCGGGCGACACCACCCt                                                                                                   >  2:125319/1‑149 (MQ=25)
                                            aTCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTAc                                                                                                 <  2:153937/149‑1 (MQ=255)
                                            aTCGGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTAc                                                                                                 <  2:410768/149‑1 (MQ=255)
                                               tGGCATCTCGGGGAGTGCACGACGGGCCGCGTTGCATCCGGGAGTTTTTGCTGCGCCAATTCAACAAGGCCATGCGTCAGGATGGCCTTTAGTATAATTAGATACCTGGCAGTGCCCTACTCTCGCATGGGGAGACCCCACACTACCAt                                                                                              <  1:338743/149‑1 (MQ=16)
                                                ggCATATGTGGGAGTATAAGACGCGCAGCGTTGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTGCGCAGAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATc                                                                                             <  2:535368/149‑1 (MQ=255)
                                                ggCATATGTGGGAGTATAAGACGCGCAGCGTCACATCAGGCATTTTTTTCTGCGTCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATc                                                                                             <  2:340657/149‑1 (MQ=255)
                                                    tatGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCCTCGgcg                                                                                         >  2:310590/1‑149 (MQ=255)
                                                       gtgGGAGTATAAGACGCGCAGCGTCGCATCCGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTAGAGAACGGCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTa                                                                                      >  2:418299/1‑149 (MQ=21)
                                                       gtgGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTTTTTTTTTTTGCAACAAGGCCCTGCGTCAGGATGTCCTTTCGCGTAATTTGATTCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTCCCGGCGGCGCTa                                                                                      >  1:781919/1‑149 (MQ=21)
                                                       gtgGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCAGCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCCTCGGCGCTa                                                                                      >  2:507553/1‑149 (MQ=255)
                                                             gTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACCCTACAATCGGCGCTACggagc                                                                                >  1:646676/1‑146 (MQ=255)
                                                              tataAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCCTGGGGAGACCCCACACTACCATCGgccggcgggcgtt                                                                               >  1:169993/1‑138 (MQ=255)
                                                               ataAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGgccccactttgggt                                                                              <  1:442770/149‑13 (MQ=255)
                                                               ataAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGttt                                                                              <  2:784316/149‑1 (MQ=255)
                                                                  aGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGCTGGCCTTTCGCATAATTTGATGCCTGGCGGTTCCCTACTCGGGCGTGGGGGGCCGGCCCACCACCATCGGCACTACGGCGCTTCag                                                                           >  1:247967/1‑148 (MQ=18)
                                                                  aGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCACCGGCCCTCCGGCGTTTCAc                                                                           >  1:693749/1‑149 (MQ=255)
                                                                  aGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTGCGGCGTTTCAc                                                                           >  2:781827/1‑149 (MQ=255)
                                                                    aCGCGCAGCGTCGCATCAGGCATTTTTGTCTGCGCCAATTTAAAAAGGCCATCCGTCCGGATAGCCTTTCGCATAATTTGTTTCCTGGCAGTTCCCTTCTCCCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACtt                                                                         <  1:263988/149‑1 (MQ=21)
                                                                     cgcgCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTc                                                                        >  2:339937/1‑149 (MQ=255)
                                                                     cgcgCAGCGTCGCATCAGGCATTTTTTTCTCTGCCGGTGCTAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTc                                                                        >  1:188247/1‑149 (MQ=25)
                                                                        gcAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGa                                                                     >  2:363298/1‑149 (MQ=255)
                                                                               cGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGc                                                              <  2:443011/149‑1 (MQ=255)
                                                                                  accAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGAATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATg                                                           <  2:366390/147‑1 (MQ=255)
                                                                                       gCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTGCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAAGGGGTc                                                      >  1:654772/1‑149 (MQ=255)
                                                                                       gCATATTTTTCTGCGCCAATGCAAAAAGTCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGAAGACCCCACACTACCATCGGCGCTGCGGCGTTTCCCTTCGGAGTCAGGCATGGGGTc                                                      >  1:490525/1‑149 (MQ=21)
                                                                                        cATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCa                                                     >  2:540748/1‑149 (MQ=255)
                                                                                        cATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCa                                                     >  2:545638/1‑149 (MQ=255)
                                                                                         aTTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAg                                                    >  2:797907/1‑149 (MQ=255)
                                                                                          tttttttCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAgg                                                   >  1:27033/1‑149 (MQ=255)
                                                                                             ttttCTGCTCCAATGGAAAAAGGCCCTCCGTCATGATGGCCTGTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTTCCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTgg                                                <  1:283544/149‑1 (MQ=17)
                                                                                             ttttCGGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTTCTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTgg                                                <  2:498271/149‑1 (MQ=255)
                                                                                                tCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGac                                             <  2:631265/149‑1 (MQ=255)
                                                                                                tCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGac                                             <  1:551641/149‑1 (MQ=255)
                                                                                                   gcgcCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGaccac                                          >  2:839764/1‑149 (MQ=255)
                                                                                                    cgcCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGaccccc                                         >  2:353202/1‑149 (MQ=255)
                                                                                                      ccAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcgc                                       >  1:315759/1‑149 (MQ=255)
                                                                                                       cAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcgcg                                      <  2:475840/149‑1 (MQ=255)
                                                                                                        aaTGCAAAAAGGCCATCCGTCAGGATGGCATTTCGCATAAATCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAAACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGGCAGGTGGGCCCCCCgcgc                                     >  1:853355/1‑149 (MQ=17)
                                                                                                         aTGCAAAACGGCCATCCGTCAGGATTGCCTTTCCGATAATTTGATGCCTGGTAGTTCCCTACTCTCGCATGGGGAGCCCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCt                                    <  1:492793/149‑1 (MQ=17)
                                                                                                         aTGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTGCTCTTGCATGGGTAGACCCAACGCTCCCTTCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCt                                    <  2:205927/149‑1 (MQ=18)
                                                                                                           gCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCCCTACGTCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAc                                  <  2:170220/149‑1 (MQ=255)
                                                                                                              aaaaGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGgc                               <  1:120615/149‑1 (MQ=255)
                                                                                                                  ggCCATCCGTCAGGATGGCATTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCAGGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTGCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGgccgcc                           <  2:652131/149‑1 (MQ=255)
                                                                                                                   gccatcctgacggATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCa                          >  2:608407/12‑149 (MQ=14)
                                                                                                                   gCCATCCGTCAGGACGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCa                          <  2:61765/149‑1 (MQ=255)
                                                                                                                      aTCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGc                       <  2:582120/149‑1 (MQ=255)
                                                                                                                         ccacaagacggCCTTTCGCATAATTTGACGCCCGGCAGTTCCCTACTCTCGCATGCGGAGACCCCACACTACCATCGGCGCCACGGCGTCTCACCTCCGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCaaa                    <  1:584337/140‑1 (MQ=11)
                                                                                                                          gTCAGGATTGCCTTTCGCATAACTTGCGGCCTGGCAGTTCCCTACGCTCGCATGGGGAGACCCCACACTTCCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAAt                   <  1:284926/149‑1 (MQ=14)
                                                                                                                           tCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTCCTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAAtt                  >  1:792173/1‑149 (MQ=255)
                                                                                                                           tCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAAtt                  >  2:588546/1‑149 (MQ=255)
                                                                                                                               gATGGCCTTTCGCTTAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGCGAGCCCCCACACGACCATCGGCGCTACGGCGTCTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGt              <  1:739191/149‑1 (MQ=11)
                                                                                                                               gATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGt              <  1:820809/149‑1 (MQ=40)
                                                                                                                                    ccTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTAt         <  1:20544/149‑1 (MQ=37)
                                                                                                                                    ccTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTAt         <  1:627348/149‑1 (MQ=37)
                                                                                                                                     cTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATc        >  1:179644/1‑149 (MQ=25)
                                                                                                                                     cTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATc        >  1:608809/1‑149 (MQ=25)
                                                                                                                                     cTTTCGCATAATTCGATGCCGGGCAGGTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATc        <  1:125319/149‑1 (MQ=255)
                                                                                                                                      tttCGCATTATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCAGCCAGGCAGGAGCTGTTTTTTCa       >  1:331285/1‑149 (MQ=11)
                                                                                                                                      tttCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCa       >  2:505431/1‑149 (MQ=25)
                                                                                                                                        tCGCATAATTCGATGCCTGGCAGTTCCGTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTATCACTTCTGAGTTCGGCATGGGGTCAGGTGGCACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCTGa     <  2:604914/149‑1 (MQ=11)
                                                                                                                                         cGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGAc    >  1:717985/1‑149 (MQ=32)
                                                                                                                                           cATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACCg  >  2:539674/1‑149 (MQ=14)
                                                                                                                                                  |                                                                                                                                             
ACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACCG  >  CP009273/2719280‑2719567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: