Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,859,846 G→C 4.4% intergenic (+22/+72) ygbN → / ← rpoS putative transporter/RNA polymerase, sigma S (sigma 38) factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,859,8460GC 4.4% 250.9 / 2.9 81intergenic (+22/+72)ygbN/rpoSputative transporter/RNA polymerase, sigma S (sigma 38) factor
Reads supporting (aligned to +/- strand):  ref base G (46/31);  new base C (2/2);  total (48/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.15e-01

TCTGGATTGTGACCAAATATCTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAAC  >  CP009273/2859700‑2859992
                                                                                                                                                  |                                                                                                                                                  
tCTGGGTTGGGACCAAATATCTGGGGGTGTCGGTAGCAGACGGTCTGAAAGCCTGGACGGTGTTAACGTCCATGCTCGGTTTTACCGGATTCTTAATTACCGGGTGGGTATGGGCGGTAATTTGACCTTAATAAAAAGGGCCGATgcgc                                                                                                                                                  <  1:374108/149‑1 (MQ=255)
tCTGGATTGTGACCAAATATCTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGc                                                                                                                                                  >  2:342718/1‑149 (MQ=255)
 cTGGATTGTGACCAAATATCTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCa                                                                                                                                                 >  2:117289/1‑149 (MQ=255)
   ggATTGTGACCAAATATCTGGGGTTGTCGGTAGCAGACGGTCTGAAAAACTGGACGGTGTTTACGCCCATTATGGGTTTTACCGGATTGTTGATCACGTGGGGCGTATGGGGGGGTATTTGTCCTTAATAAAAAGGACCGATGGGCATc                                                                                                                                               >  1:507572/1‑149 (MQ=255)
    gATTGTGGCTGAATATCTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCg                                                                                                                                              <  2:178720/149‑1 (MQ=255)
      ttGTGACCAAATATCTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTGATTTGACCTTAATAAAAAGGTCCGATGGGAATCGGa                                                                                                                                            >  2:107901/1‑149 (MQ=255)
        gtgCCCAAATATCAGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATCACCTGGTGCGTATGGCCGGTCATTTGACATCACTAAAACCGTCTGAAGCGCATcttcct                                                                                                                                          <  1:640421/149‑6 (MQ=255)
        gtgACCAAATATCTGGCGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGAcc                                                                                                                                          <  2:141612/149‑1 (MQ=255)
         tgACCAAATATCTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCt                                                                                                                                         >  2:525703/1‑149 (MQ=255)
          gACCAAATATCTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCtt                                                                                                                                        >  2:157335/1‑149 (MQ=255)
          gACCAAATATCTGGGGTTGTCGGGAGCGGGCTGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAGTTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCtt                                                                                                                                        <  1:378599/149‑1 (MQ=255)
             cAAGTATCTGGGGTTGTCGGTAGCAGACGGTCTGAAACCCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTa                                                                                                                                     <  1:165790/149‑1 (MQ=255)
             cAAATATCTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTGCCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGCCCATCGGACCttttt                                                                                                                                     <  2:853416/149‑2 (MQ=255)
             cAAATATCTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTa                                                                                                                                     <  2:79836/149‑1 (MQ=255)
               aaTATCTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTGTTAtt                                                                                                                                   <  1:608702/149‑1 (MQ=255)
                 tatCTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATtgt                                                                                                                                 >  1:661050/1‑149 (MQ=255)
                   tCTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATCAAAAGGTCCGATGGGCGTCGGACCTTTTATTGTGc                                                                                                                               >  2:703384/1‑149 (MQ=255)
                    cTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGca                                                                                                                              <  1:780297/149‑1 (MQ=255)
                    cTGGGGTTGTCGGTAGCAGACGGTCAGAAACCCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGca                                                                                                                              >  2:449534/1‑149 (MQ=255)
                      gggtTTGTCGGTAGCAGACGGTCTGAAAACCGGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGGGCGTATGGGCGGTAATTTGACCTTAATAAAAATGTCCGATGGGCATCGGACCTTTTATTGTGcaca                                                                                                                            <  2:557591/149‑1 (MQ=255)
                      ggggTTGTCGGTAGCAGACTGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGcaca                                                                                                                            <  2:588983/149‑1 (MQ=255)
                      ggggTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGAATCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTAGTGTGcaca                                                                                                                            >  1:5647/1‑149 (MQ=255)
                       gggTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTCATTTGCCCTTAATAAAAAGGGCCGATGGGCATCGGACCTTTGATTGTGCCCAg                                                                                                                           >  2:187706/1‑149 (MQ=255)
                       gggTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATGAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAg                                                                                                                           >  2:616821/1‑149 (MQ=255)
                                             tGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTgaga                                                                                                     >  2:460854/1‑149 (MQ=255)
                                                    cTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCt                                                                                              >  2:800652/1‑149 (MQ=255)
                                                        aCAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGGCTGGCCTTTCt                                                                                          >  1:455472/1‑149 (MQ=255)
                                                        aCAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGCCCGGCCTTTCt                                                                                          >  1:72557/1‑149 (MQ=255)
                                                        aCAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCt                                                                                          >  1:867841/1‑149 (MQ=255)
                                                         cAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTg                                                                                         <  1:842968/149‑1 (MQ=255)
                                                          aGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGa                                                                                        <  2:852584/149‑1 (MQ=255)
                                                            tgtTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGGCa                                                                                      >  2:28086/1‑149 (MQ=255)
                                                            tgtTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGGCCGATGGGCATCGGACCTTTTATTGTGCACGGAAAAGGCCAGCGTCGCTTGAGACTGGCCTTTCTGACa                                                                                      >  2:142440/1‑149 (MQ=255)
                                                                   gACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTACTTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGctt                                                                               <  1:525703/149‑1 (MQ=255)
                                                                   gACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGctt                                                                               >  2:519643/1‑149 (MQ=255)
                                                                   gACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGctt                                                                               >  2:88589/1‑149 (MQ=255)
                                                                   gACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGctt                                                                               >  1:525443/1‑149 (MQ=255)
                                                                   gACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAAGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATActt                                                                               >  1:232397/1‑149 (MQ=255)
                                                                              gTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTACTATTCACTGAAAAGGCCGGCCTCGCTTGAGACTGGCCTTTCTGACAGCTGCTTACCTACTcgcg                                                                    >  1:94893/1‑149 (MQ=255)
                                                                                 ttACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGaa                                                                 <  2:700182/149‑1 (MQ=255)
                                                                                  tACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAAc                                                                <  2:335689/149‑1 (MQ=255)
                                                                                   aCCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGCTGCTTACTTACTCGCGGAACa                                                               >  2:823654/1‑149 (MQ=255)
                                                                                     cGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAgc                                                             >  1:382516/1‑149 (MQ=255)
                                                                                     cGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAgc                                                             >  2:719463/1‑149 (MQ=255)
                                                                                          tCTTAACTACCTGGTGCGTAGGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTc                                                        <  1:215054/149‑1 (MQ=255)
                                                                                            ttattacccctgcgCGTATGGGTGCGAAGTTCACCTTCATAATACCGTCCGCTGGGCATCGGACATCTTATTGTCCACCGAAAACGCCACCCTCGCTTGATACCGGCCTTTCTGACAGATGCTTACTTCCTCGCGGAACAGCGCTTCGa                                                      <  1:303834/136‑1 (MQ=255)
                                                                                            ttAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGa                                                      <  2:675352/149‑1 (MQ=255)
                                                                                              aaTTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGATTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAAAAGCGCTTCGata                                                    >  1:506709/1‑149 (MQ=255)
                                                                                              aaTTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGACATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGata                                                    >  1:278702/1‑149 (MQ=255)
                                                                                                   ccTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAg                                               <  2:867841/149‑1 (MQ=255)
                                                                                                          ggttatggcggcAATTTGACCTTACGAAAAAGGTCCGACGGCCATACGACCTTTTATTGTGCCCAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGc                                        <  2:789052/143‑1 (MQ=255)
                                                                                                           gTATGGGCGGTAATTTGACCTTAATAAAGAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTGGAGACTGGCCTTCCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCg                                       >  2:466276/1‑149 (MQ=255)
                                                                                                              tGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGttt                                    >  1:586846/1‑149 (MQ=255)
                                                                                                              tGGGCGGGAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGttt                                    <  2:19337/149‑1 (MQ=255)
                                                                                                                 gcggtaaccataCCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCa                                 >  1:228118/12‑149 (MQ=255)
                                                                                                                 gCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTCTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCa                                 <  1:616821/149‑1 (MQ=255)
                                                                                                                 gCGGTAAGTGGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACACGCGGAGCAGCGCTTCGATATTCAGCCCCTGCGTTTGCa                                 <  1:187706/149‑1 (MQ=255)
                                                                                                                  cGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATCTTCAGCCCCTGCGTTTGCAg                                >  1:788309/1‑149 (MQ=255)
                                                                                                                  cGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAg                                >  1:410182/1‑149 (MQ=255)
                                                                                                                    gTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGa                              >  1:137841/1‑149 (MQ=255)
                                                                                                                        tttGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGCTTGCAGGATTTc                          >  1:454182/1‑149 (MQ=255)
                                                                                                                           gACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTcgcg                       >  2:18972/1‑149 (MQ=255)
                                                                                                                              cTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCaa                    >  1:606661/1‑149 (MQ=255)
                                                                                                                              cTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCaa                    >  2:786687/1‑149 (MQ=255)
                                                                                                                              cTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTCCGTTTGCAGGACTTCCCGCaa                    >  2:215185/1‑149 (MQ=255)
                                                                                                                              cTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGAACAGAAAAGGCCAGCATCGATAGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCaa                    >  1:185376/1‑149 (MQ=255)
                                                                                                                               cacaataaaaGGTCCGATGCCCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCaaa                   >  1:305953/7‑149 (MQ=255)
                                                                                                                                 aataaAAGCGGCCGGTCGGCATGGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACg                 <  1:461592/149‑1 (MQ=255)
                                                                                                                                 aataaAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAAGAGCGCTTCGATATTCAGCCCCTGAGGTTGAAGGATGTCGCGCAAACg                 >  2:50017/1‑149 (MQ=255)
                                                                                                                                 aataaAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGCTATTAAGCCTCTGCGTTTGCAGGATTTCGCGCAAACg                 >  2:116462/1‑149 (MQ=255)
                                                                                                                                    taaaaGGTCCGATGCCCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGgcg              >  2:187082/2‑149 (MQ=255)
                                                                                                                                    aaaaaGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGgcg              <  1:785648/149‑1 (MQ=255)
                                                                                                                                      aaaTGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGACAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCa            >  1:553184/1‑149 (MQ=255)
                                                                                                                                      aaaGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCa            <  2:142885/149‑1 (MQ=255)
                                                                                                                                      aaaGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCa            <  2:142828/149‑1 (MQ=255)
                                                                                                                                      aaaGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCa            <  2:13756/149‑1 (MQ=255)
                                                                                                                                      aaaGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCa            >  2:127780/1‑149 (MQ=255)
                                                                                                                                      aaaGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCa            <  2:463290/149‑1 (MQ=255)
                                                                                                                                      aaaGGCCCGATGGGCATCGGACCTTTGATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCa            <  2:507572/149‑1 (MQ=255)
                                                                                                                                         ggTCCGTTGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGc         <  1:342718/149‑1 (MQ=255)
                                                                                                                                          gTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGcc        >  1:534104/1‑149 (MQ=255)
                                                                                                                                                tGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAAc  <  2:814299/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TCTGGATTGTGACCAAATATCTGGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCAGGCCTTCAAC  >  CP009273/2859700‑2859992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: