Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,049,411 G→T 14.8% Q97K (CAG→AAG)  glnL ← sensory histidine kinase in two‑component regulatory system with GlnG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,049,4110GT14.8% 141.9 / 15.2 60Q97K (CAG→AAG) glnLsensory histidine kinase in two‑component regulatory system with GlnG
Reads supporting (aligned to +/- strand):  ref base G (31/20);  new base T (7/2);  total (38/22)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

CCAAGCGGATTTTTAATCTCATGTGCCAGGCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTA  >  CP009273/4049263‑4049553
                                                                                                                                                    |                                                                                                                                              
ccAAGCGGATTTTTAATCTCATGTGCCAGGCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTg                                                                                                                                                <  2:528899/149‑1 (MQ=255)
 cAAGCGGACTTTTAATCTCATGTGCCAGGCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTgg                                                                                                                                               <  1:516109/149‑1 (MQ=255)
         tttttAATCTCATGTGCCAGGCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTcac                                                                                                                                       <  1:54079/149‑1 (MQ=255)
         tttttAATCTCATGTGCCAGGCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTcac                                                                                                                                       >  2:648269/1‑149 (MQ=255)
                         ccAGGCCGCGCACTACATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGAAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAg                                                                                                                       >  1:548417/1‑149 (MQ=255)
                         ccAGGCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAg                                                                                                                       >  2:643831/1‑149 (MQ=255)
                         ccAGGCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAg                                                                                                                       <  1:423262/149‑1 (MQ=255)
                           aGGCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAgcg                                                                                                                     >  1:663238/1‑149 (MQ=255)
                           aGGCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGAAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAgcg                                                                                                                     >  1:139520/1‑149 (MQ=255)
                            ggCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCGGGATCAGGCCGTCCGGCGTCCGCTGGGCGGTCACAGACAGGATATGCGAgcgc                                                                                                                    >  2:165581/1‑149 (MQ=255)
                            ggCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAgcgc                                                                                                                    >  2:470839/1‑149 (MQ=255)
                            ggCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAgcgc                                                                                                                    >  1:7195/1‑149 (MQ=255)
                                 cgcACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCCCAGAAAGGATATGCGAGCGCCCGTc                                                                                                               >  2:742086/1‑149 (MQ=255)
                                 cgcACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGtg                                                                                                               >  2:190511/1‑148 (MQ=255)
                                    aCTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGAt                                                                                                            >  2:537188/1‑149 (MQ=255)
                                       aagtcccGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGAc                                                                                                         <  1:791361/143‑1 (MQ=255)
                                          tCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTACTGACTTAAGCGGCGCTGGTTATCCATCGGCGCCATTTCCGACAGGCTCATGCCGTCCGGCAAACGCTTGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAg                                                                                                      >  1:46166/1‑149 (MQ=255)
                                           cACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTTGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGc                                                                                                     <  2:809785/149‑1 (MQ=255)
                                             cGGGCAGCACCCTGCGGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGt                                                                                                   <  1:72315/149‑1 (MQ=255)
                                                        ctgctgGGCGTGCGTGAGCTGGTCCTGGCTTAAGCGGCGCTGGTTATCCGGCGGAGCGATCGGCAGCGGGATCAGGCCGGCCGGCAGACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTAt                                                                                        <  2:179275/149‑1 (MQ=255)
                                                         tgctgGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGTGTCACTTCGTTATc                                                                                       >  1:281539/1‑149 (MQ=255)
                                                          gctgGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCg                                                                                      <  1:108319/149‑1 (MQ=255)
                                                                cGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTaaaa                                                                                >  1:563146/1‑149 (MQ=255)
                                                                        aGCGGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGccc                                                                        <  1:381062/149‑1 (MQ=255)
                                                                            gTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGTGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGcc                                                                    <  1:258369/149‑1 (MQ=255)
                                                                             ttCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGCGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTTCCCCGCCt                                                                   >  1:123501/1‑149 (MQ=255)
                                                                             ttCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCt                                                                   >  1:483240/1‑149 (MQ=255)
                                                                             ttCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCGCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCt                                                                   >  1:700472/1‑149 (MQ=255)
                                                                                cTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTTGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAACCCCTGCGCCGCCTCCa                                                                >  1:135871/1‑149 (MQ=255)
                                                                                cTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTccc                                                                >  2:150828/1‑148 (MQ=255)
                                                                                cTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCAATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCa                                                                >  2:705453/1‑149 (MQ=255)
                                                                                  gccTTAAGCGGCGCTGGTTACCCATCGGAGCCATCTCCAGCAGGATCATGCCGCCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGa                                                              <  2:48162/147‑1 (MQ=255)
                                                                                  gACTTCAGCGCCGCTGGTTCTCCATCGGGGCCATCTCCAGCAGGTTCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATAGGCGAGCCCCCGTCGATGACCACCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGa                                                              <  1:121319/149‑1 (MQ=255)
                                                                                   aCTTAAGCGGCGCTGGTTACCCATCGGACACATCCCCCGCAGGATCATGCCGCCCGGCATACGCTGGGCCGTCACAGAGAGGATATGCGAGCTCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGAc                                                             <  1:507185/149‑1 (MQ=255)
                                                                                      tAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTTGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACttt                                                          >  2:807208/1‑149 (MQ=255)
                                                                                      tAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAACCCTTGCCCCGCCTCCAGACttt                                                          <  1:509509/149‑1 (MQ=255)
                                                                                        cgcggcgCTGGTTTTCCATCGGTGCCATCTCCAGCAGGATCATGCCGTCCGGCATAGGGTGGGCCGGCACAGAAAGGATATGCGAGCGCCCTCCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCt                                                        <  1:696889/148‑1 (MQ=255)
                                                                                         gcggcgCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGAGATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCtt                                                       <  2:828108/149‑1 (MQ=255)
                                                                                           ggcgCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATCCGCTGGGCCGGCACAGAAGGGATGTGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGc                                                     >  2:492259/1‑149 (MQ=255)
                                                                                           ggcgCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGc                                                     <  2:7195/149‑1 (MQ=255)
                                                                                           ggcgCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGc                                                     >  2:473309/1‑149 (MQ=255)
                                                                                           ggcgCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGc                                                     <  1:470839/149‑1 (MQ=255)
                                                                                           ggccCTGGTTACCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGCCACAGAAAGGACATGCGAGCGCCCGTCGATGACCACCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGc                                                     <  1:165581/149‑1 (MQ=255)
                                                                                             cgcTGGTTATCCATCGGAGCCATCTCCGGCAGGATCATGCCGTCAGGCAGACGGTGGGCCGTCACAGAAAGGATATGCGAGGGCGCGTCGGTGACCAGCGTGGCTTCGGTGTCGGTAAAACCTTGCCCAGCCCCCAGCCTTTCTTGcct                                                   >  1:651292/1‑147 (MQ=255)
                                                                                                 ggTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGc                                               >  2:865654/1‑149 (MQ=255)
                                                                                                   ttATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTTGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTc                                             >  2:863678/1‑149 (MQ=255)
                                                                                                         aTCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTTGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGGTAtt                                       >  2:701417/1‑149 (MQ=255)
                                                                                                                ccATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTCAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATgag                                >  1:810222/1‑149 (MQ=255)
                                                                                                                  gtctcCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGaa                              >  1:636768/2‑149 (MQ=255)
                                                                                                                    ctcCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGt                            <  1:154917/149‑1 (MQ=255)
                                                                                                                     tcCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTACAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCGCGATGTTTAAGGAgtagta                           >  1:673098/1‑149 (MQ=255)
                                                                                                                       cagcagGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGc                         >  2:645099/1‑149 (MQ=255)
                                                                                                                       cagcagGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGc                         >  2:830221/1‑149 (MQ=255)
                                                                                                                        agcagGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCt                        >  1:182975/1‑149 (MQ=255)
                                                                                                                          cagGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTACATCAGCTCGATATTTAATGAGAAGTAGCtga                      >  1:244633/1‑147 (MQ=255)
                                                                                                                           cagcgtatgccggacGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCaa                     >  2:104159/15‑149 (MQ=255)
                                                                                                                           agGATCATGCCGTCCGGCATACGCTTGGCCGTCACAGAACGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCaa                     <  2:208150/149‑1 (MQ=255)
                                                                                                                                  tGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTcc              >  1:556653/1‑149 (MQ=255)
                                                                                                                                     cGTCCGGCATACGCTTGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGt           >  2:609174/1‑149 (MQ=255)
                                                                                                                                          ggCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCAATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTATCgg      <  1:705453/149‑1 (MQ=255)
                                                                                                                                             aTACGCTTGGCCGTCACAGAAAGGATATGCGAGCGCGAGTCGATGACCAGCGCCAATTCGTTATCGTTAAAACCTGGCCCCGCCTCCAGACTTTCTTGCAACAGGTCGATATTAAATGAGAAGTAGCTCACCAGTTCCGTTAACGgagt   >  1:316901/1‑149 (MQ=255)
                                                                                                                                              tgcGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGCCCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTa  <  2:676295/147‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
CCAAGCGGATTTTTAATCTCATGTGCCAGGCCGCGCACTAAATCACGGGCAGCAACCTGCTGGGCGTGCTGTAGCTGTTCCTGACTTAAGCGGCGCTGGTTATCCATCGGAGCCATCTCCAGCAGGATCATGCCGTCCGGCATACGCTGGGCCGTCACAGAAAGGATATGCGAGCGCCCGTCGATGACCAGCGTCACTTCGTTATCGGTAAAACCTTGCCCCGCCTCCAGACTTTCTTGCATCAGCTCGATATTTAATGAGAAGTAGCTCAACAGTTCCGGTAACGGTGTA  >  CP009273/4049263‑4049553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑