Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 468,288 A→T K79* (AAG→TAG)  glnK → nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273468,2880AT98.0% 414.6 / NA 149K79* (AAG→TAG) glnKnitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (92/54);  minor base G (1/2);  total (93/56)
Fisher's exact test for biased strand distribution p-value = 5.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 149 total aligned reads displayed.

CACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAG  >  CP009273/468143‑468431
                                                                                                                                                 |                                                                                                                                               
cACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCTTTTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAgg                                                                                                                                              <  2:1185719/149‑1 (MQ=255)
cACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAgg                                                                                                                                              >  2:937382/1‑149 (MQ=255)
  ccGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAggcg                                                                                                                                            <  2:1342667/149‑1 (MQ=255)
  ccGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAggcg                                                                                                                                            <  2:2636894/149‑1 (MQ=255)
  ccGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAggcg                                                                                                                                            >  1:1441631/1‑149 (MQ=255)
  ccGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACAGGGGGGCGGAATACAGAGTAAATTTGCTGCCAAAAGTAAAAATTGATGTGGCGATTGATGATGACCAACTCGATGAACTGCTTGATATGGTAGGTTAggcg                                                                                                                                            >  1:1741428/1‑149 (MQ=255)
   cGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTAACGGGGGGCGGAATACAGAGCCAAGTTCCTTCCTGAAGTGGAAATGGATATGGCGATTGCGGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAggcgg                                                                                                                                           >  1:671964/1‑149 (MQ=255)
        tGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGACGAAGTGATCGATATCGTCCGTTAGGCGGCttcc                                                                                                                                      >  1:2465319/1‑147 (MQ=255)
        tGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATGGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTac                                                                                                                                      >  1:1637806/1‑149 (MQ=255)
          aaaGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAATGGATCGATATCGTCCGTTAGGCGGCTTaccc                                                                                                                                    >  1:1121416/1‑147 (MQ=255)
          aaaGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTacac                                                                                                                                    >  2:1884575/1‑149 (MQ=255)
          aaaGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTacac                                                                                                                                    >  2:190915/1‑149 (MQ=255)
          aaaGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATAGCGTCAGTTGGGCGGCTTactc                                                                                                                                    >  1:2201036/1‑147 (MQ=255)
           aaggtgtgagttctCAGAAAGGGCATGCGGAGCTCTACCTGCGTGCGGAATACTGCGTCAATTTCCTGCCAAAATTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTTCTCTATATTTTTAGTTAGCCGGCGTACAcc                                                                                                                                   <  1:2045161/136‑1 (MQ=255)
            aGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCttgcttcg                                                                                                                                  <  1:1654890/149‑7 (MQ=255)
            aGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCg                                                                                                                                  >  2:1399441/1‑149 (MQ=255)
              gTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGa                                                                                                                                >  2:132767/1‑149 (MQ=255)
              gTTTCGGGCGCCAGAAAGGGCATGCCGAGCCGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGa                                                                                                                                <  2:436838/149‑1 (MQ=255)
               tttCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGaa                                                                                                                               <  1:1270400/149‑1 (MQ=255)
                  cGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACGGCGCCATTTGCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGaaaaa                                                                                                                            <  2:1166898/149‑1 (MQ=255)
                      cGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTAATCAATGTTATTAGTTAGGCGGCTTACACCGGAAAAATTgg                                                                                                                        <  2:1966645/149‑1 (MQ=255)
                        tCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAACAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATCGGCg                                                                                                                      >  1:101568/1‑149 (MQ=255)
                        tCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTTGGCGGCTTACACCGGAAAAATTGGCg                                                                                                                      >  1:2471932/1‑149 (MQ=255)
                        tCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCg                                                                                                                      >  2:1591259/1‑149 (MQ=255)
                          aGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTGCCGGCCAAAAGTAAAAAGTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATAACGTCTGTTAGGCGGCTTAAAAAAAAAAAATTGGCGAc                                                                                                                    <  1:1233611/149‑1 (MQ=255)
                           gAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAATATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATAGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACg                                                                                                                   >  2:1738665/1‑149 (MQ=255)
                           gAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACg                                                                                                                   >  2:2014657/1‑149 (MQ=255)
                               gggCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAACAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACCCCGGAAAAATTGGCGACGGCaa                                                                                                               >  2:413263/1‑149 (MQ=255)
                                ggCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCaaa                                                                                                              >  2:1048550/1‑149 (MQ=255)
                                ggCAAGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCaaa                                                                                                              <  2:2223145/149‑1 (MQ=255)
                                  cATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGACGGTTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGACTTACACCGGAAAAATTGGCAACGGCAAAAt                                                                                                            >  1:1063889/1‑149 (MQ=255)
                                     gCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCtt                                                                                                         >  1:1109932/1‑149 (MQ=255)
                                         aGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTtcgtc                                                                                                     <  2:640198/149‑1 (MQ=255)
                                           cTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGc                                                                                                   >  1:350981/1‑149 (MQ=255)
                                            tgaaaccgggggCGGAAGACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGAGGTGGCGATCGCTGATGACCCAGTGGATGAAGTGATCGATATCGTCAGTTAGGCAGCTTACACCGGAAATATTGGCGACGGCAAAATCTTCGTCGCt                                                                                                  <  2:536798/142‑1 (MQ=255)
                                              tACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACGCCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGa                                                                                                >  1:562927/1‑149 (MQ=255)
                                              tACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGa                                                                                                >  1:1400395/1‑149 (MQ=255)
                                                 cGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGCTGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAAtt                                                                                             >  1:1618440/1‑149 (MQ=255)
                                                  ggggggCGGGAGACAGCTGCACTTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGGTGAGGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTg                                                                                            <  1:1722933/149‑1 (MQ=255)
                                                     gggCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCGGAATTGCaa                                                                                         >  1:1944182/1‑149 (MQ=255)
                                                      ggCGGAATACAGCGTCAAGTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGCCGAAGGCAAAATCTTCCTCGCCGCATTGCAAc                                                                                        >  1:1723131/1‑149 (MQ=255)
                                                        cGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAAcgc                                                                                      >  1:2316486/1‑149 (MQ=255)
                                                              aCAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGCCGGCAAAATCTTCGTCGCTGAATTGCAAAGCGTcttg                                                                                >  1:2328598/1‑146 (MQ=255)
                                                                  cGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGCCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCattcgta                                                                            >  1:709253/1‑149 (MQ=255)
                                                                   gTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCACCGCGTCattggtat                                                                           >  2:239858/1‑149 (MQ=255)
                                                                   gTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCattcgtat                                                                           >  2:2380244/1‑149 (MQ=255)
                                                                    tCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCattcgtatt                                                                          <  2:945081/149‑1 (MQ=255)
                                                                     gatttttCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGAGGACCAACTCGAGGAAGTGATCGATATCGTCAGCTACGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCattcgtattc                                                                         <  1:2591230/146‑1 (MQ=255)
                                                                     cAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCGTACACAGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCACCGCGTCattcgtatac                                                                         >  2:377139/1‑147 (MQ=255)
                                                                       aTTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGCCattcgtgttcgt                                                                       >  1:1194296/1‑149 (MQ=255)
                                                                             tgacAAAAGTGAAAATTGTTGTGGCGCTGGCTGATGGCCAACTCGATGAAGTGATCGCAGCCGTCAGTTAGGCGGCCTCAACCGGTAAAATTGGCGAGGGCAAAATCTTCGACGCTTAATTGCAACGCGTCTTTCGTATTCGTACCGGc                                                                 <  1:177888/146‑1 (MQ=255)
                                                                             tGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGc                                                                 >  2:2098214/1‑149 (MQ=255)
                                                                             tGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGCCATTCGTATTCGTACCGGc                                                                 >  1:693907/1‑149 (MQ=255)
                                                                              gCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGGCATTCGTATTCGTACCGGCg                                                                >  1:148205/1‑149 (MQ=255)
                                                                               ccAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGa                                                               >  2:1775474/1‑149 (MQ=255)
                                                                                    aGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTCGGCGGCTTACACCGGAACAATCGGCGACGGCGAAATCTGCGTCTCTGAATTGCAACGCGACATTCGTATTCGTACCGGCGAAGCcg                                                          >  1:1638599/1‑149 (MQ=255)
                                                                                     gTAAAAATTGATGTGGCGATTGCTGATGACCAACGCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCcga                                                         <  2:2266880/149‑1 (MQ=255)
                                                                                      gcaaaaTTGTTTGGGCGATTGCTGATTACACACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAATTCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCcgac                                                        <  1:2400938/147‑1 (MQ=255)
                                                                                       aaaaaTTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGGCGCTGAATTGAAACGCGTCATTCGTATTCGTACCGGCGAAGCcgacg                                                       >  1:1797967/1‑149 (MQ=255)
                                                                                          gaTTCATTTGTCGACTGCGGCTGACCAACTCGAGGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAAGTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAg                                                    <  1:1352730/148‑1 (MQ=255)
                                                                                            ttGATCTGGCGATCGCGGATGCCCAACTCGATGAAGTGATCGCTCTCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAgcg                                                  <  2:208863/149‑1 (MQ=255)
                                                                                                tgtgGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGgcgc                                              <  2:2310069/149‑1 (MQ=255)
                                                                                                  tgGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTg                                            >  2:1086972/1‑149 (MQ=255)
                                                                                                  tgGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTg                                            >  2:1668869/1‑149 (MQ=255)
                                                                                                    gCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTa                                          >  1:1809048/1‑149 (MQ=255)
                                                                                                    gCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGGTAGAAGTCATACACCGTAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGAGGAAGCGGCGCTGTa                                          >  1:443782/1‑149 (MQ=255)
                                                                                                      gATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAAt                                        <  1:638160/149‑1 (MQ=255)
                                                                                                      gATTGCCGATGACCAACTCGATGAAGTGATCGATAGCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTGCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAAt                                        <  2:630389/149‑1 (MQ=255)
                                                                                                         tgcgtatgaCCAACCCGAGGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAAGTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCCGTAAtctc                                     <  1:982397/144‑1 (MQ=255)
                                                                                                         tGCTGAGGATCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCCTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAAtctc                                     <  1:573755/149‑1 (MQ=255)
                                                                                                          gCTGATCACCCACTCGATGAAGGGAGCGATATCGGCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGACGCGGCGCTGTAATctct                                    <  1:1642007/149‑1 (MQ=255)
                                                                                                            tgatgaCCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAGATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCGACGCGTCATGCGTATTCGTACCGGCAAAGCCGACTAAGGGGCGCTGTagtagcggg                                  >  1:2069668/1‑141 (MQ=255)
                                                                                                            tgatgaCCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTgg                                  >  2:1018690/1‑149 (MQ=255)
                                                                                                            tgatgaCCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCCTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTgg                                  <  1:132767/149‑1 (MQ=255)
                                                                                                            taaggcccAACTGGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGGCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTgg                                  <  1:747639/143‑1 (MQ=255)
                                                                                                              atgaCCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGca                                >  2:2597644/1‑149 (MQ=255)
                                                                                                               ggaCCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAATTCTTCGGCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGcac                               <  1:1618016/148‑1 (MQ=255)
                                                                                                                gaCCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAGCGCGTCGTTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGcaca                              >  2:738640/1‑149 (MQ=255)
                                                                                                                gaCCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGGGGTAATCCCTGCcaca                              >  1:1078742/1‑149 (MQ=255)
                                                                                                                gaCCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTCACACCGGAAAAATTGGCGACGGCAAAATCATCGTCGCTGAATTGCAACGCGTAATTCGTATTCGTACCGGAGAAGCCGACGAAGCGGCGCGGTAACCTCTGGcaca                              >  1:544305/1‑149 (MQ=255)
                                                                                                                  ccATCTCGCTGAGGGGATGGGTATCGTCAGTTGGGCGGCTTACACCGGAAAAATTGGCGACGGCAAATTCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGTAGCCGACGAAGCGGCGCTGTAATCTCTGGcacaca                            <  1:1175120/149‑1 (MQ=255)
                                                                                                                   cAACTCGATGAAGTGATCGATATCGTCAGCTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAg                           >  2:1617919/1‑149 (MQ=255)
                                                                                                                      cTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCaa                        >  2:210697/1‑149 (MQ=255)
                                                                                                                       tCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAAc                       >  1:1482789/1‑149 (MQ=255)
                                                                                                                        cGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGCCGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACa                      >  1:516424/1‑149 (MQ=255)
                                                                                                                        cGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACa                      <  2:562927/149‑1 (MQ=255)
                                                                                                                        cGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAAGTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTATCGGCGAAGCCGACTAAGCGGCGCTGTAATCTCTGGCACACAGCAACa                      <  2:1716240/149‑1 (MQ=255)
                                                                                                                            gAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGaa                  <  2:2104314/149‑1 (MQ=255)
                                                                                                                            gAAGTGATCGATAACGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGaa                  <  2:2328598/149‑1 (MQ=255)
                                                                                                                             aatgtaTCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCCACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAAc                 <  2:791570/144‑1 (MQ=255)
                                                                                                                             aaGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAAGGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGGAATCTCTGGCACACGGGCACAGGAAc                 >  1:209594/1‑149 (MQ=255)
                                                                                                                             aaGTGATCGATATCGTCAGTTAGGCGGCTTACACAGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAAc                 >  1:2444244/1‑149 (MQ=255)
                                                                                                                                 gATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCTACAGGAACGaaa             >  1:1925297/1‑149 (MQ=255)
                                                                                                                                 gATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGaaa             <  2:1637806/149‑1 (MQ=255)
                                                                                                                                  aTCGATATCGTCAGTTAGGCGCCTTACACCGGAAAAATTGGCGCCGGCAAAATCTTCGTCGCTGAATTGCACCGCGTACTTCGTAGTCGTGCCGGCCAAGCCGCCAAAGCGGCGCTGTAAGCCCCGGCCCACCCCCTCAGGACCGaaac            >  1:81484/1‑148 (MQ=255)
                                                                                                                                   tCGATATGCTCGGTGAGGCGGCTTACACCGGAAAAATTGGCAACGGCAAAATCTTCGTCGCTGAATTGCAACGCTTCATTCGTATTCGTACCGTCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGaaaaa           <  2:1741428/149‑1 (MQ=255)
                                                                                                                                      atatCGTCAGTTAGGCGGCTTACACCGTAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGa        <  2:1388667/149‑1 (MQ=255)
                                                                                                                                       tatCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGaa       <  2:126194/149‑1 (MQ=255)
                                                                                                                                          cGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGGAGAt    >  2:400177/1‑149 (MQ=255)
                                                                                                                                            tCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAg  >  1:2648338/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAG  >  CP009273/468143‑468431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: