Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,421,172 A→C D33E (GAT→GAG argT ← lysine/arginine/ornithine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,421,1720AC97.5% 463.5 / NA 164D33E (GAT→GAGargTlysine/arginine/ornithine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base C (80/79);  minor base G (3/1);  total (84/80)
Fisher's exact test for biased strand distribution p-value = 6.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 164 total aligned reads displayed.

TCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTT  >  CP009273/2421027‑2421317
                                                                                                                                                  |                                                                                                                                                 
gcggtggtgcgttCATCGCGTCAAAGTCACGGGCAACCCGGGACCATTTCACCTGCATCCGTTTGCAAATCTCGTTACCGAGATCGATATCAAGGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCg                                                                                                                                                <  1:2093037/137‑1 (MQ=255)
       ggggATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCTATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGa                                                                                                                                         <  2:1948558/149‑1 (MQ=255)
        gggATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATAACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTGTCGGTTCCggt                                                                                                                                        >  2:84228/1‑147 (MQ=255)
         ggATCTTCGCGTCAAAGTCACTGGCAACCCAGGTACATTTATCCTGAATGCGTTGACAGATTGCGTTACCGAGGTCGTTGAAAAATCCGACAGATGCATCTTTATTATCGTTCAGTGAGAGCGGTTCGTAGGTGGTCTCGGTTCCGATa                                                                                                                                       <  1:1244065/149‑1 (MQ=255)
          gATCAGCGCGTAAAAGTCATTGGCAACCCAGGTATATTTCACCTGCATCCGTTTGCACATCTCGTTACAGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATAc                                                                                                                                      <  2:777468/149‑1 (MQ=255)
              aGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAACCACACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTAc                                                                                                                                  <  2:1415422/149‑1 (MQ=255)
                 gcgTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTAGCGAGAGCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGGGAACGGTGGGTAGGTGGTCTCGGTTCCGATGCGTACCGt                                                                                                                               >  2:1552597/1‑149 (MQ=255)
                     cAAAGTCACTGGCAACCCAGGTACATTTCGCCTCCATCCGGTTGCACAACTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGACCGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTcc                                                                                                                           <  2:2345809/149‑1 (MQ=255)
                      aaaGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATCCGTACCGTCTCCg                                                                                                                          >  2:1494135/1‑149 (MQ=255)
                       atgTCACTGGCAACCCAGGTACATTGCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCCTTCGATGAGAACGGTGCGTAGGTCGTCTCGGTTCCGATACGTACCGTCTCCgg                                                                                                                         <  1:2336259/147‑1 (MQ=255)
                        aGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATAAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGtttacgaa                                                                                                                        >  2:914823/1‑142 (MQ=255)
                            aCTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTAGCGAGATCGATCTCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCCCGGTTCCGACAGGCACCGTCACCGGTAgca                                                                                                                    >  1:639858/1‑148 (MQ=255)
                             cTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAgcgc                                                                                                                   <  2:256695/149‑1 (MQ=255)
                              tGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGACCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGcgcc                                                                                                                  >  1:1427655/1‑149 (MQ=255)
                                    cccTGGTACATTTCACCTGGATTCGTTTGCACCTCCCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAg                                                                                                            <  1:2591992/149‑1 (MQ=255)
                                    cccAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAg                                                                                                            >  2:2229555/1‑149 (MQ=255)
                                     ccAGGTACATTCCACCTGCATCCGTTTGCACATCTCGTAACCGAGAGCGATACCAAAGCCAACAAAATCACCTTTAGCCTCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGc                                                                                                           <  1:1976661/149‑1 (MQ=255)
                                      cacgTACATTTCACTTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCt                                                                                                          <  1:1904756/146‑1 (MQ=255)
                                        ggTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGGACCGTCTCCGGTAGCGCCGCATAGCTgg                                                                                                        <  1:216419/149‑1 (MQ=255)
                                         gTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGa                                                                                                       >  2:2303671/1‑149 (MQ=255)
                                          tACATTTCACCTGCCGCCGTCTGGACTTCGGGGTACCGAGACCGTTATCAAACTCAAGCAAATCACCTTTAGCATCTTGCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACATACCGTCTCCGGTAGCGCCGCATAGCTGGaa                                                                                                      <  1:2420041/149‑1 (MQ=255)
                                          tACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGaa                                                                                                      >  1:2169351/1‑149 (MQ=255)
                                           actgttCACCTGGCTCCGTCTGCACATCTCGTTACCGAGATCGATAGCAAAGCCAACAAAATCACCTTGCGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAg                                                                                                     <  1:422483/145‑1 (MQ=255)
                                           aCATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCCCCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAg                                                                                                     <  1:1305675/149‑1 (MQ=255)
                                           aCATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATTGCTGGCAg                                                                                                     >  2:37681/1‑149 (MQ=255)
                                           aCATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAg                                                                                                     <  2:181891/149‑1 (MQ=255)
                                           aCATTTCACCTGCATCCGTTTGCACATCCCGTTACCGAGATCGATATCAAAGCCAACAAACTCACCTTTAGCATCTTTCGATGAGAACGGTGCGCAGGTGGTCGCGGTTCCGGTCCATCCCGTCGCAAGTAGCGCCGCAGCACTGGAAg                                                                                                     >  1:1495487/1‑149 (MQ=255)
                                            cATTTCCGCTGCCTCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAACATCTCCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGc                                                                                                    <  1:182308/149‑1 (MQ=255)
                                            cATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGCCTCCGGTAGCGCCGCATAGCTGGAAGc                                                                                                    >  2:1884204/1‑149 (MQ=255)
                                               ttCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGc                                                                                                 >  2:1541816/1‑149 (MQ=255)
                                                   ccTGCATCCGTTTGCACATCTCGTTACCGAGATCCATTTCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCtgtg                                                                                             <  2:1155174/149‑1 (MQ=255)
                                                    cTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCCAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGCTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCCCCTAGCTGGCAGcaggtaggc                                                                                            >  2:2368804/1‑141 (MQ=255)
                                                      gCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCTATGAGAACGGTGCGTAGATGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCACCGCATAGCGGGAACACGGTGTGgag                                                                                          >  1:1085719/1‑149 (MQ=255)
                                                      gCATACGTATGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGgag                                                                                          <  2:2121971/149‑1 (MQ=255)
                                                            gTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGCGAGACCg                                                                                    >  2:319296/1‑149 (MQ=255)
                                                            gTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGACGCCTAGCTGGAAGCCGCCGTGGAAAGAACg                                                                                    >  1:1127152/1‑149 (MQ=255)
                                                             tttGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGCTGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATGCGTACCGTCTCCGGTAGCGCCGCATAGCTGGCAGCCGCCGTGGGGAGACCgc                                                                                   >  1:756647/1‑148 (MQ=255)
                                                              ttGCCCATCCCGTTAGCGAGATCGATATCAAAGTCTACGAACTCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGAc                                                                                  <  1:1086898/149‑1 (MQ=255)
                                                              gtGCACATCCCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGAc                                                                                  <  2:1450527/148‑1 (MQ=255)
                                                               tGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACt                                                                                 <  2:1447633/149‑1 (MQ=255)
                                                               tGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACt                                                                                 <  2:2252801/149‑1 (MQ=255)
                                                                 cacaTCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTaa                                                                               >  2:1176827/1‑149 (MQ=255)
                                                                 cacaTCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTaa                                                                               <  2:515281/149‑1 (MQ=255)
                                                                  acaTCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAAc                                                                              >  2:1005893/1‑149 (MQ=255)
                                                                  acaTCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGGGGAGAGACCGACTAAc                                                                              >  1:206982/1‑149 (MQ=255)
                                                                    ttctcGGTAACGAGATCGATATCAAAGCCAACAAAAACAGCTTTAGCATCTTCCGATGAGAACGGTGAGTAGATGGTCTCGGTTCCGCTATGTACCGTCTCGGTAAGCGCCACGTAGCTGGAAGCCGCGGGGGAGGGCCCGAccaccca                                                                            >  1:398585/2‑143 (MQ=255)
                                                                    aTCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGCCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGCAAGCCGCCGCGGCGAGACCGACTAACaa                                                                            >  1:1805496/1‑149 (MQ=255)
                                                                    aTCTCGTCACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGGCACGTACCGTCTCCGGTAGCGCGGGATAGCTACACACAGCTCCTGAGAGACCGAATAAcca                                                                            >  1:2542450/1‑147 (MQ=255)
                                                                     tctcGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTGCGATGAGAACGGTGCGGAGGTGGTCCCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGAAAGCCGCAGTGGAAAGACCGAcaaacaaa                                                                           >  1:2343889/1‑149 (MQ=255)
                                                                     gctcGTTCCCGAGATCGATATCAAAGCCAAAAAAACTCGCCTGAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTAGAAGCCGCCGTGGAGAGACCGACTAACaaa                                                                           <  2:1392154/148‑1 (MQ=255)
                                                                     cccccttACCGAGATCGATATCAAAGCCAACAAAATCCCCTTTAGCTGCTTTCGATGAGAACGGTGCACGGTTGGGCTCCGTTCCTTTCCCTACGGGCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACaaa                                                                           <  2:2628909/144‑1 (MQ=255)
                                                                       tcGTTACCGAGATCGATATCAAAGCCAACAAAATCCACTTCAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCGGAGCAGCGACCGACAAACAAAGa                                                                         >  1:2568615/1‑149 (MQ=255)
                                                                       tcGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCCTCGTTCGATGAGAACGGTGCGTAGATGGGCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCAGCTGTGGAGAGAGCGACTAACAgaga                                                                         >  1:2659014/1‑149 (MQ=255)
                                                                          ttACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGTTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGCAGCCGCTGTGGAGAGCCCGACTAACAAAGCCag                                                                      >  1:487260/1‑149 (MQ=255)
                                                                           tACCGAGATCGATATCAAAGCCAACAAAATCACCTTTCGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGCCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACaga                                                                     <  1:1767718/149‑1 (MQ=255)
                                                                            aCCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTCGGGCCGCGTAGCTGGTAGCCGCTGTGGAGAGACCGACTAACAAAGACagag                                                                    >  2:2195786/1‑149 (MQ=255)
                                                                              cGAGATCGATATCAAAGCCAACAAAATCAACTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTGTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGGGAGACCGACTAACAAAGACAGAGCg                                                                  >  2:1635037/1‑149 (MQ=255)
                                                                                  aTCGATATCAAAGCCAAAAACATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGGGACCGACTAACAAAGACAGAGCGAGaa                                                              >  1:907645/1‑149 (MQ=255)
                                                                                    tgATATCAACGCCACCCAAAGCACCGTTGGCGCCTTTCGATGAGAACGGTGCGTAGTTGGTCCCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATc                                                            <  1:379891/148‑1 (MQ=255)
                                                                                      atatCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGGGCGAGAATCGa                                                          >  1:2178867/1‑149 (MQ=255)
                                                                                       tatCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAGCGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACCGCACGAGAATCgcc                                                         >  1:1664726/1‑147 (MQ=255)
                                                                                       taccAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGGGCGTAGGTGGCCGCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGAc                                                         <  2:1109859/146‑1 (MQ=255)
                                                                                         tCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGGAGCCGCCGTGGAAAGACCGACTAACAAAGACAGAGCGAGAATCGCctt                                                       >  1:935755/1‑149 (MQ=255)
                                                                                         tCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGActt                                                       <  2:1103924/149‑1 (MQ=255)
                                                                                         tCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGAAGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGActt                                                       >  2:435940/1‑149 (MQ=255)
                                                                                            acgCCAACAAAATCACCTTGACCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCCCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGActtctt                                                    <  2:2465520/147‑1 (MQ=255)
                                                                                            aaGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGActtctt                                                    >  1:2108524/1‑149 (MQ=255)
                                                                                              gCCAACAAAATCACCTTTAGCATCTTTCGCTGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAATGACAGAGCGAGAATCGACTTCTTca                                                  >  2:1631823/1‑149 (MQ=255)
                                                                                               ccAACAAACTCACCTTTAGCATCTTTCGATGGGAACGGTGCGTGGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTaat                                                 <  1:2458936/149‑1 (MQ=255)
                                                                                               ccAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcat                                                 <  2:2418250/149‑1 (MQ=255)
                                                                                                caacaaGATCCCCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcata                                                <  1:84228/149‑1 (MQ=255)
                                                                                                 aacaaAATCCCCTTTAGCATCTTTCGATGAGACCGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcatac                                               <  1:914823/149‑1 (MQ=255)
                                                                                                 aacaaAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCGGCTGTGGAGAGACCGACTAACAAAGACAGAGAGAGAATAGAGTTGTTcctac                                               >  1:2257097/1‑149 (MQ=255)
                                                                                                 aacaaAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcatac                                               >  2:864007/1‑149 (MQ=255)
                                                                                                 aacaaAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcatac                                               >  2:704796/1‑149 (MQ=255)
                                                                                                  acaaAATCACCTTTAGCATCTTTCGATGAAACCGATCATTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGACTCCACTTCTTcataca                                              >  1:1233306/1‑149 (MQ=255)
                                                                                                   caaAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcatacat                                             >  2:2426967/1‑149 (MQ=255)
                                                                                                       atcatcTTTAGCTGCTTTCGATGAGACCGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGCAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAAcc                                         <  1:2493952/149‑1 (MQ=255)
                                                                                                           acttttAGCATCTTTCGATGGGAACGGTGCGTAGCTGGTCTCCGCTCCGATACGGACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTc                                      <  1:701287/146‑1 (MQ=255)
                                                                                                           aCCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGACACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTc                                      <  1:2278216/149‑1 (MQ=255)
                                                                                                             cTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGTTCTCGGTTCCGCTACGTACCGTCTCTGGTGGCGCCGCATAGCTGGGAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGGGAGAATCGCCTTCTTCGTACATAACCCTCag                                    >  2:446664/1‑148 (MQ=255)
                                                                                                             cTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATATATAACCCTCaa                                    <  1:2147861/149‑1 (MQ=255)
                                                                                                             cTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACAAACAAAGAAGGAGCGAGAATCGACTTCTTCATCGATAACCCTCaa                                    >  1:1063418/1‑149 (MQ=255)
                                                                                                               gtAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGt                                  <  1:492696/148‑1 (MQ=255)
                                                                                                                tcgCATCTTTCGATGAGAACGGTGCGGAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTg                                 <  2:313863/147‑1 (MQ=255)
                                                                                                                tAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTg                                 <  2:175653/149‑1 (MQ=255)
                                                                                                                 aGCATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGa                                <  2:2492513/149‑1 (MQ=255)
                                                                                                                   cATCTTTCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGACAAAGGACGGCGCCGGTAGCCCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGaaa                              >  2:2631093/1‑149 (MQ=255)
                                                                                                                     tCTTCCGATGAGAACGGTGCGTAGGTGGTCTCGGTCCCGACACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAAt                            <  2:71774/149‑1 (MQ=255)
                                                                                                                        ttCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGc                         <  1:2178058/149‑1 (MQ=255)
                                                                                                                        ttCGATGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGc                         <  2:2116308/149‑1 (MQ=255)
                                                                                                                             tGAGAACGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTATCAATGCCAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCttttt                    <  2:2509527/149‑1 (MQ=255)
                                                                                                                               agaACGGTGCGTAGGGGGTCACGGTTCCGATACGTACCGCCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTa                  <  2:1270951/149‑1 (MQ=255)
                                                                                                                                 aaCGGTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGGGAAAATGGCTCTTTTAtg                >  2:1742214/1‑149 (MQ=255)
                                                                                                                                     gTGCGTAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGAGACCTATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTAtgttgt            <  2:206982/149‑1 (MQ=255)
                                                                                                                                          tAGGTGGTCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGAAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGGTGTGTAtt       >  2:1783019/1‑149 (MQ=255)
                                                                                                                                          tAGGTGGTCTCGGTTCCGATACGTACCGTCTACGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGCGTTTTTTAGGTTGTGTAtt       >  1:1903914/1‑149 (MQ=255)
                                                                                                                                               ggtGTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTAttgtgtt  <  1:1852918/149‑1 (MQ=255)
                                                                                                                                               ggtCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTAttgtgtt  >  1:1425375/1‑149 (MQ=255)
                                                                                                                                               ggtCTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTAttgtgtt  <  1:1005893/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTT  >  CP009273/2421027‑2421317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: