Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,737,874 G→A W54* (TGG→TAG)  mdtK → multidrug efflux system transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,737,8740GA100.0% 114.6 / NA 41W54* (TGG→TAG) mdtKmultidrug efflux system transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/15);  total (26/15)

AGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAGGTGCGACAAGGTTTCTGGCTGGCAGGTTTTGTTTCC  >  CP009273/1737729‑1738010
                                                                                                                                                 |                                                                                                                                        
aGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCGATCGGTACTTCTATCTAGCt                                                                                                                                       >  2:674064/1‑149 (MQ=255)
  tGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTAGCTTc                                                                                                                                     >  1:15845/1‑149 (MQ=255)
    aaGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCAAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTCTCGGTACTTCTATCTAGCTTCCg                                                                                                                                   >  1:143668/1‑149 (MQ=255)
     aGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTAGCTTCCgg                                                                                                                                  <  2:644750/149‑1 (MQ=255)
      gcgcGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGCTGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGAGGCGGTCGCTATCGGTAGTTCTGTCTAGCTTCTGGc                                                                                                                                 >  1:457379/1‑149 (MQ=255)
                 attagcattagcAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTAGCTTCCGGCGATCCTCTTTg                                                                                                                      >  1:69906/1‑149 (MQ=255)
                     gcattagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTAGCTTCCGGCGATCCTCTTTGGTCa                                                                                                                  <  1:664395/149‑1 (MQ=255)
                      catttgcaATCCCGGTGATTCTCACGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATAGCGTGATGGCGGGCGGCTTTAGTGTCACCGACCTGGCGGCGGTCGCTATCGGTACTTCTATCTAGCGTCCGGCGATCCTCTTTGGTCAc                                                                                                                 <  2:727459/149‑1 (MQ=255)
                           gcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTAGCTTCCGGCGATCCTCTTTGGTCACGGCct                                                                                                            >  2:652395/1‑149 (MQ=255)
                                 ccGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGACACCGACATGGAGGCGGGCGCTCTCGGTACTTCTATCTAGCTTCCGGCGATCCTCTTTGGTCACGGCctgcggcg                                                                                                      >  2:532881/1‑148 (MQ=255)
                                  cGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTAGCTTCCGGCGATCCTCTTTGGTCACGGActgctgctg                                                                                                     >  1:332310/1‑149 (MQ=255)
                                   ggTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTAGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTgg                                                                                                    <  2:573081/149‑1 (MQ=255)
                                    gTGATTCTCGCGCAAATCGCCCGAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTAGCTTCCGGCGATCCTCTTTGGTCACGGACTGATGCTGGc                                                                                                   >  2:324286/1‑149 (MQ=255)
                                    gTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTAGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGc                                                                                                   <  2:466977/149‑1 (MQ=255)
                                     tGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTAGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGCa                                                                                                  <  2:46630/149‑1 (MQ=255)
                                           tcGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTAGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGCATTAACg                                                                                            >  1:223248/1‑149 (MQ=255)
                                             gcgcAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTCTCTAGCTTCCGGCGATCCTCTTTGGTCACGGACTGGTGCGGGCATTacagcg                                                                                          >  2:571763/1‑144 (MQ=255)
                                               gcAAATCGCCCAAACTGCGATGGGTTTTGTCGGTACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGTTATCGGTACTTCTATCTAGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGCATTAACGCCgg                                                                                        <  2:412869/149‑1 (MQ=255)
                                                        ccAAACTGCGATGGGTTTTGTCGATGCCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTAGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGCATTAACGCCGGTTATCgcgc                                                                               >  1:177964/1‑149 (MQ=255)
                                                        ccAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTAGCTTCCGGCGATACTCTTTGGTCACGGACTGCTGCTGGGATTAAGGCCGGTTAtggggc                                                                               >  1:184878/1‑144 (MQ=255)
                                                           aaCTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTAGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTTCTGGCATTAACGCCGGTTATCGCGCAAt                                                                            >  1:570753/1‑149 (MQ=255)
                                                            aCTGCGATGGGTTTTGTCGATACCGTGATGGCGCGCGACTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTAGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGCATTAACGCCGGTTATCGCGCAAtt                                                                           >  1:469042/1‑149 (MQ=255)
                                                               gcgtgggtgtttGTCGCTACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTAGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGCATTAACGTCGTTTCTCGCGCAATTaaa                                                                        <  2:637723/140‑1 (MQ=255)
                                                                  aTGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTAGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGCATTAACGCCGGGTATCGCGCACTTCAATgg                                                                     >  2:717770/1‑149 (MQ=255)
                                                                     ggTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTAGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTc                                                                  >  1:73095/1‑149 (MQ=255)
                                                                       ttttGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTAGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCg                                                                >  1:73561/1‑149 (MQ=255)
                                                                       ttttGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTAGCTCCCGGCGATCCTCTTTGGTCACGGACTGCTGCAGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCg                                                                <  1:106818/149‑1 (MQ=255)
                                                                             cGATACCGTGGTGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTAGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGAc                                                          <  2:650566/149‑1 (MQ=255)
                                                                             cGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTAGCATCCGGCGATCCTCTTTGGTCCCGGGCTGCTGCTGGCATTAACGCCGGCTATCGCGCAATTAAATGGTTCCGGCCGAc                                                          >  2:44634/1‑149 (MQ=255)
                                                                               aTACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACGTCTATATAGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGCATTAACGCCGGTTATCGCGGCATTAAAGGGTTGCGGGCGACGt                                                        >  2:423130/1‑149 (MQ=255)
                                                                                  ccGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTAGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAg                                                     <  1:486792/149‑1 (MQ=255)
                                                                                    gcgagggcgggcgGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTAGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAgcg                                                   <  2:143668/144‑1 (MQ=255)
                                                                                       aTGGCGGGCGGCTATAGTGCCTCCGACATGGCGGCGGTCGCTGTCGGTACTTCTCTCTAGCTTCCGGCGATCCTCTTTGGTCACGGTCTTCTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCAt                                                <  2:165843/149‑1 (MQ=255)
                                                                                       aTGGCGGGCGGCTATAGTGCCACCCACATGGCGGCGGCCGATATCCGTCCTTCTATCTAGCTTCCGGCGATCCTCTTTGGTAACGGTCTGCCGTTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCAt                                                <  1:247342/149‑1 (MQ=255)
                                                                                          gcgggcggCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTAGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGGGCGCATTgc                                             >  1:196245/1‑149 (MQ=255)
                                                                                                  cTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTAGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAgg                                     >  1:222957/1‑149 (MQ=255)
                                                                                                      aGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTAGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAGGTGCg                                 >  1:438200/1‑149 (MQ=255)
                                                                                                        tGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTAGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAGGTGCGAc                               >  2:81317/1‑149 (MQ=255)
                                                                                                          ccACCGACATGGCGGCGGTCGCTATCGGCACTTCTATCTAGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAGGTGCGACaa                             >  2:320804/1‑149 (MQ=255)
                                                                                                                  aTGGCGGCGGTCGCTATCGGTACTTCTATCTAGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGCCTTAACGCCGGCTATCGCGCAATTAAATGGTTCCGGTCGACGCGACCGCCTTGCGCATCAGCTGCGACAAGGCTTctg                     >  2:303226/1‑149 (MQ=255)
                                                                                                                                     gTACTTCTATCTAGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAGGTGCGACAAGGTTTCTGGCTGGCAGGTTTTGTTTcc  <  2:15845/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                        
AGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGACGTGAGCGCATTGCGCATCAGGTGCGACAAGGTTTCTGGCTGGCAGGTTTTGTTTCC  >  CP009273/1737729‑1738010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑