Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	CP009273	542,271	IS5 (–) +4 bp	coding (136‑139/1668 nt)	fdrA →	putative NAD(P)‑binding acyl‑CoA synthetase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	269666 =	NA (NA)	25 (0.590)	23/296	1.0	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	CP009273	= 542274	0 (0.000)					coding (139/1668 nt)	fdrA	putative NAD(P)‑binding acyl‑CoA synthetase
*	?	CP009273	= 270860	NA (NA)	62 (1.460)	47/296	0.0	100%	noncoding (320/1221 nt)	IS30	repeat region
	?	CP009273	542271 =	0 (0.000)					coding (136/1668 nt)	fdrA	putative NAD(P)‑binding acyl‑CoA synthetase

ACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTT  >  CP009273/269518‑269812                                                                                                                                                     |                                                                                                                                                   aCTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAACGCCAGAAACTTCGCTGCATCGCTGACAGCACTGTTATg                                                                                                                                                    <  1:372851/149‑1 (MQ=255)   tAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGa                                                                                                                                                  >  1:85489/1‑149 (MQ=255)   tAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAAGAGAAAGCCAGAAACCTCGCCGCATCGTTGACAGCACTGTCATGGa                                                                                                                                                  >  1:571861/1‑149 (MQ=255)     aaaTCCCCCGTGTTAATGACGGCTTTATCGTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTAGGGCGAGATGCGAGTGGTCTCAACGATCCGGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAg                                                                                                                                                <  2:536456/149‑1 (MQ=255)         ccccccTGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTTATTCGCAAACGCAGTCGTTATGGCGAGATGCCAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGc                                                                                                                                            <  2:175920/149‑1 (MQ=255)           cccATTGTAATGACGGCGTTACCCTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGa                                                                                                                                          <  2:415935/149‑1 (MQ=255)            ccATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCCCTGTTATGGAAGGTGCgga                                                                                                                                         >  1:354957/1‑147 (MQ=255)             cATGTTAATGACGGCTTTATCTTTCTGGGTCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGGGATGCGAGTGGGCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAAt                                                                                                                                        >  2:511694/1‑149 (MQ=255)                   aaTGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCgg                                                                                                                                  >  2:363374/1‑149 (MQ=255)                    gcggcGGCCTTATCTTTCTGGGGCACAGGCTCATTCGCACACGCAGTCGGTATGGCGAGATGCGCGTGGTCCCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCggg                                                                                                                                 <  2:223991/145‑1 (MQ=255)                          gCTTTATGCTGCTGGGGCACAGGCTCATTCGCAAACTCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCATAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAAtt                                                                                                                           <  1:306667/149‑1 (MQ=255)                           cTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTCTGGGAGGTGCGGATAAGGGGGGGAAttc                                                                                                                          >  2:104122/1‑149 (MQ=255)                              tATCTTTCTGGGGCATAGGCTCATTCGCAAACGCAGTCGTTATGGCGCGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATACGCGGGGAAAttcttc                                                                                                                       >  2:312329/1‑149 (MQ=255)                              tATCTTTATGGGCCACAGGCTCATTCACAAACGCAGTCGTTGTGGCGAGCTGCGAGTGGACTCAACGATCCCGAAAGGAAAAGCCAGAAACTTCGCCGACTCGATAAAAGAACTGTTATGCCAGGTGCGAATAAGCGGGGAAAttctta                                                                                                                       >  2:187613/1‑148 (MQ=255)                                 cTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGCTCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCg                                                                                                                    >  1:170108/1‑149 (MQ=255)                                   ttCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGc                                                                                                                  <  2:52604/149‑1 (MQ=255)                                    tCTGGGGCACAGGCTCATTCGCAACCGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCt                                                                                                                 <  1:470820/149‑1 (MQ=255)                                      tGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGa                                                                                                               >  2:297594/1‑149 (MQ=255)                                        gggCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACt                                                                                                             >  2:314634/1‑149 (MQ=255)                                          gcacgcgCTCATTCGCCAACCCCGTCTGTATGGCGAGCTGCTAGGGGTCTCAACTATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCa                                                                                                           <  2:173163/143‑1 (MQ=37)                                          gCAAAGGCTCATTCGACAACGCTGTCGTTAGGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCa                                                                                                           <  2:173185/149‑1 (MQ=38)                                                 cTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcattt                                                                                                    <  1:519331/149‑1 (MQ=255)                                                  tCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGGCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTCTGGAAGGTGCGAATAAGCGGGGAAAGTCTTCTCGGATGACTCAGtcctttc                                                                                                   >  2:366369/1‑149 (MQ=39)                                                    aTTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTAAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcatttcat                                                                                                 <  1:641101/149‑1 (MQ=255)                                                          aaaCGCAGTCGTTATGGCGAGATGCGGGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGCATAAGCGGGGAGATCCTTCTCGGCTGGCACACTCATTTCATttcttc                                                                                           >  2:462965/1‑149 (MQ=255)                                                              gCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGt                                                                                       <  2:371920/149‑1 (MQ=255)                                                                 gTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTg                                                                                    >  2:208354/1‑149 (MQ=255)                                                                     ttATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGcc                                                                                >  1:582539/1‑149 (MQ=255)                                                                       aTGGCGAGATGCGAGTGGTCTCAACGAGCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGAGAATAAGCGGGGCAATTCTTCTGGGCTGACTCAGGCATTTCATTTCTGAATGTTTggtacga                                                                              >  2:376026/1‑143 (MQ=255)                                                                          gCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGCAATTCTTCTCGGCTGACTCCGTCATTTCATTTCTCCACGTTTGAGCCgctct                                                                           >  2:442930/1‑145 (MQ=37)                                                                            gagaTGCGAGTGGTCTCCACGATCCCGCAGGAAAATGCCAGAAATTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGACATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAttttt                                                                         <  1:128535/149‑1 (MQ=25)                                                                             ggacgCGAGCGGTCGCAACGGGCCCGCAGCAGAAACCCAGAACCTTCGCCGAATCGCTGACAGCACTGTGATGGAAGGTGCGAATAAGCGGGGCAATTCTCCTCGGCTGACCCAGTCATTTCACTCCTCCATGTTTGACCCGAttcctt                                                                        <  2:370356/145‑5 (MQ=18)                                                                                tGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCCTTTCTTCATGTTTGAGCCGATTTTTtctc                                                                     <  1:421648/149‑1 (MQ=255)                                                                                tGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtctc                                                                     <  1:402979/149‑1 (MQ=255)                                                                                     ggggTCTCAACGCTCCCGCGAGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTa                                                                <  2:281415/147‑1 (MQ=255)                                                                                     gTGGTCCCAGCGATCCCGCACGACAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTa                                                                <  2:281409/149‑1 (MQ=255)                                                                                      tGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTaa                                                               >  1:375693/1‑149 (MQ=255)                                                                                          ctcAACGATCCCGCAGGAGAAAGCCAGAAACTCCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGc                                                           <  1:646553/149‑1 (MQ=255)                                                                                           tcAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGcc                                                          >  1:625768/1‑149 (MQ=255)                                                                                           tcAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATCTTTTCTCCCGTAAATGcc                                                          >  2:641579/1‑149 (MQ=255)                                                                                             aaCGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCtt                                                        <  1:277632/149‑1 (MQ=255)                                                                                                gATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGCAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGaa                                                     <  2:217422/149‑1 (MQ=255)                                                                                                 aTCCCGCAGGAGAAAGCCAGACACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAAt                                                    <  1:312329/149‑1 (MQ=255)                                                                                                   cccGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCa                                                  <  2:141034/149‑1 (MQ=255)                                                                                                                  cAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAgcacgt                                   >  2:78851/1‑144 (MQ=255)                                                                                                                                 aTCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGACATGTTATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTaa                    <  1:682266/149‑1 (MQ=255)                                                                                                                                     cTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCg                >  1:26019/1‑149 (MQ=40)                                                                                                                                       gACAGCACTGTAAGGGAAGGTGCGAAGAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTCCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCgct              <  2:354957/149‑3 (MQ=255)                                                                                                                                        aCAGCACTGTTATGGAACGTGCGAATAAGCGGGGAAATTCTTCTCGGCTAACTCAGTCATACAATTTCTTCATCCTTGAGCCGCTTTTTTCTCCCGTAAATGCCTTGAATCAGCCTCTTTAGACCGTTTCTTCGCCATTTCCGGCGTTa             >  2:101063/1‑149 (MQ=11)                                                                                                                                         cAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTAt            <  1:104122/149‑1 (MQ=37)                                                                                                                                              cTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCa       <  2:625768/149‑1 (MQ=32)                                                                                                                                               tGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAg      >  1:221647/1‑149 (MQ=32)                                                                                                                                               tGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCCTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAg      <  2:656014/149‑1 (MQ=17)                                                                                                                                                   aTGGAAGGTGCGAATAAGCGGGGAAATTCTTCCCAGCTGACCCAGTCATTTCATTTCTTCCTGTATGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCCATTTAGACCGGTTCTTCGCCATTTAAGGCGTTATCCACAGtttt  >  2:164422/1‑149 (MQ=11)                                                                                                                                                     |                                                                                                                                                   ACTAAAATCCCCCATGTTAATGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTT  >  CP009273/269518‑269812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.