Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,860,429 Δ1 bp coding (482/993 nt) rpoS ← RNA polymerase, sigma S (sigma 38) factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,860,4270T.99.2% 568.1 / ‑2.4 126coding (484/993 nt)rpoSRNA polymerase, sigma S (sigma 38) factor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base . (61/64);  minor base G (1/0);  total (62/64)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01

Only 100 of 131 total aligned reads displayed.

CGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCA  >  CP009273/2860279‑2860570
                                                                                                                                                     |                                                                                                                                               
cGGTTGACGGCATCAACTTGATTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGt                                                                                                                                                 <  1:1771314/149‑1 (MQ=255)
  gCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGttg                                                                                                                                               <  2:1412602/149‑2 (MQ=255)
   cTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGttgg                                                                                                                                              <  1:203886/149‑3 (MQ=255)
     gACGTCATCAACTGTCTTATCCACTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATGGGACAACTCTCGTGCGCTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGttggtt                                                                                                                                            <  2:1344836/149‑5 (MQ=255)
           atcaACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAAt                                                                                                                                     >  2:1673318/1‑149 (MQ=255)
           atcaACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAAt                                                                                                                                     >  1:1906737/1‑149 (MQ=255)
            tcaACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATc                                                                                                                                    <  1:1043300/149‑1 (MQ=255)
              aaCTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATGGGACAACTCACGTGCGGTTTGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGc                                                                                                                                  <  1:1731295/149‑1 (MQ=255)
                cTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGccc                                                                                                                                <  2:315061/149‑1 (MQ=255)
                cTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGCCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGccc                                                                                                                                <  1:817821/149‑1 (MQ=255)
                 tGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCg                                                                                                                               >  1:209438/1‑149 (MQ=255)
                   gtttATCCAGTTGCGCTGCGATCTCCTCCGCACTTGGTTCATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGtt                                                                                                                             <  2:336398/147‑1 (MQ=255)
                   gCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGtt                                                                                                                             <  2:27655/149‑1 (MQ=255)
                      tATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATGGGACAACTCACGTGCGATTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCaa                                                                                                                          >  1:1342009/1‑149 (MQ=255)
                       aTCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTACGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAAt                                                                                                                         <  2:499004/149‑1 (MQ=255)
                        tCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCCATc                                                                                                                        >  2:884928/1‑149 (MQ=255)
                           aGTCGCTCTGCGATCTCTTCCGCACTTGGTTCATGGCCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTc                                                                                                                     <  2:330493/149‑1 (MQ=255)
                             tggCTCTGCGACCTCTTCCGCACTGGGTGCATGGTCCAGCTTATGGGACAACTCCGGGCGTGTCCTCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTg                                                                                                                   <  1:947673/147‑1 (MQ=255)
                               gCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGc                                                                                                                 >  1:707892/1‑149 (MQ=255)
                                 tctGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGa                                                                                                               >  2:526245/1‑149 (MQ=255)
                                   tGCGCTCTCTTCCGGCCTTGGTCCATGGTCCCGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGCTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAAt                                                                                                             <  1:28886/149‑1 (MQ=255)
                                   tGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATGGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGCAt                                                                                                             >  1:1034266/1‑149 (MQ=255)
                                   tGCGATCCCTTCCGCACTGGGTTCATGGTCCAGCTTATGGGACAACTCACGTGCGGGTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAAGAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAAt                                                                                                             <  2:95151/149‑1 (MQ=255)
                                    gCGATTTCTTCCGCACGTGGTTCATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATc                                                                                                            <  2:1840855/149‑1 (MQ=255)
                                    gCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGCATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATc                                                                                                            >  2:945223/1‑149 (MQ=255)
                                         ctctTCCGCACTTGGTTCATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATccacca                                                                                                       >  2:399490/1‑149 (MQ=255)
                                            ttCCGCACTTGGTTCATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATGGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGGTCAATCGGCGGGCGCATCCACGAgat                                                                                                    >  2:797423/1‑147 (MQ=255)
                                            ttCCGCAATTGGTGCATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTGAACGTTCAGCTCCTTGACGATGTGAATCGGCAAACGAATAGTACGGGGTGGTTCATAATCGCCCGTTCAAGCGTCTGGCGAATcccgccggt                                                                                                    >  2:660819/1‑142 (MQ=255)
                                             tCCGCACTTGGTTCATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGtt                                                                                                   <  1:1673318/149‑1 (MQ=255)
                                              ccacACTTGGTTCATGGTCCAGCTCATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTGCTTAACGATGGTAATCGGCAAACGACGAGGACGGGTTGGTTCATCAGCGCCACTTCCACCGTCTGGCGAAtacgcccggctg                                                                                                  >  2:706023/4‑138 (MQ=255)
                                              ccGCACTTGGTTCATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGtta                                                                                                  >  2:1247261/1‑148 (MQ=255)
                                                   cTTGGTTCATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTAt                                                                                             <  2:209438/149‑1 (MQ=255)
                                                   cTTGGTTCATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTAt                                                                                             <  1:1231224/149‑1 (MQ=255)
                                                      ggTTCATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGtt                                                                                          <  2:1171611/149‑1 (MQ=255)
                                                       gTTCATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAGCGCATCGTACGGGTTGGTTCATAAACGCCCGTTCAATCGTCTGGCGGAACCACGCGGGTGCGTAtagtg                                                                                         >  2:79183/1‑145 (MQ=255)
                                                        ttCATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCGCGTTCAATCGTCTGGCGAAGCCACCAGGTTGCGTATGTTga                                                                                        >  1:1871399/1‑149 (MQ=255)
                                                         tCATGGTCCAGCTTATGGGACAACTCACGTGCAGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTgag                                                                                       <  2:1107625/149‑1 (MQ=255)
                                                          cATGGTCCAGCTTATGGGACAACTCGCGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTgaga                                                                                      <  2:314099/149‑1 (MQ=255)
                                                               tCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCGGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCATCTGCCGAATCCAGCAGGCTGCGCATGTTGAGAAGCgg                                                                                 >  2:974878/1‑149 (MQ=255)
                                                               tCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGACCGTACACTCGTCTGGTGAATCCACCAGGTTGCGTATGTTGAGAAGCgg                                                                                 >  1:1337598/1‑149 (MQ=255)
                                                                ccAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGa                                                                                >  2:444456/1‑149 (MQ=255)
                                                                ccAGCTTAGGGGACAACTCACGTGCGGTCCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGa                                                                                <  1:410682/149‑1 (MQ=255)
                                                                  aGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGaaa                                                                              <  2:675267/149‑1 (MQ=255)
                                                                          gtacacCTCACGGGCGGTTCGCAGGTAAAGGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTCCAAGCGTCTTGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTc                                                                      <  2:1740676/147‑1 (MQ=255)
                                                                          ggACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGGATCGGCAACCGAATAGTAAGGGGTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAACAGAGCAGGTAGCAGATCTTGAGAAGCGGAAGCCACGTTc                                                                      >  2:1232461/1‑149 (MQ=255)
                                                                             cAACTCTCGTGCTGTTCGCAGGTAAACGCTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGCTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCgg                                                                   <  1:85902/149‑1 (MQ=255)
                                                                              aaCTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCggg                                                                  <  2:1920113/149‑1 (MQ=255)
                                                                               aCTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCAAGTTCCgggg                                                                 >  1:1231703/1‑148 (MQ=255)
                                                                                cTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGta                                                                >  2:1345687/1‑148 (MQ=255)
                                                                                cTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTc                                                                <  2:725577/149‑1 (MQ=255)
                                                                                 tCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGAACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCa                                                               >  1:556856/1‑149 (MQ=255)
                                                                                   aCGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCaaa                                                             <  1:106159/149‑1 (MQ=255)
                                                                                   aCGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCaaa                                                             <  1:399490/149‑1 (MQ=255)
                                                                                   aCGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCaaa                                                             <  2:1670574/149‑1 (MQ=255)
                                                                                     gTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGt                                                                                           >  2:1536307/1‑117 (MQ=255)
                                                                                     gTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGt                                                                                           <  1:1536307/117‑1 (MQ=255)
                                                                                     gTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACt                                                           >  2:1545754/1‑149 (MQ=255)
                                                                                     gTGCGGATCGCAGGTAAACATTCAGCTACTTTACGGTGTTGATGGGCAGGCGAAGAGGACGGGTTGTGTCATAGTCGGCCGTTCAATGGTCTGGCTGATCAACCAGGTTGCGTATGGTGAGACGCGGAAACCACGTTCCGGGTCacgca                                                           >  2:1835007/1‑145 (MQ=255)
                                                                                         ggTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTtctc                                                       >  1:1349605/1‑149 (MQ=255)
                                                                                         ggTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTtctc                                                       <  1:934353/149‑1 (MQ=255)
                                                                                         ggTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGAGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTgctc                                                       >  1:679184/1‑149 (MQ=255)
                                                                                         cgcttCGCAGGGAACCTTCCAGCTTCTTTACGATGTGAATCGCGAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCGGGCGAATCCACCAGGTTGCGTATGGTGAGAAGCGGAAACCACGTTCCGGGTCAAACTtct                                                        <  2:1183685/146‑1 (MQ=255)
                                                                                           gTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTctct                                                      >  1:627688/1‑149 (MQ=255)
                                                                                             tCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGGTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCAGGGTCAAACTTCTCTAc                                                    >  2:1835731/1‑149 (MQ=255)
                                                                                                cAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTGTGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTCCTCTACcgc                                                 >  1:916041/1‑149 (MQ=255)
                                                                                                   gTAAACGTTCGGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACcgcgcg                                              <  2:102212/149‑1 (MQ=255)
                                                                                                   gTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAAACGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAGGCGGAAACCACGTTCCGGGTCAAAATtccctcccccccg                                              >  1:873768/1‑144 (MQ=255)
                                                                                                       aCGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATc                                          >  1:1544167/1‑149 (MQ=255)
                                                                                                       aCGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATc                                          <  2:627688/149‑1 (MQ=255)
                                                                                                        cGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCa                                         <  2:1906737/149‑1 (MQ=255)
                                                                                                         gTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGGCAAACTTCTCTACCGCGCGGATCAg                                        <  1:1940944/149‑1 (MQ=255)
                                                                                                                  cTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGtt                               <  1:1296629/149‑1 (MQ=255)
                                                                                                                  cTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACATCTCTACCGCGCGGATCAGCCCCAGGtt                               <  2:1937540/149‑1 (MQ=255)
                                                                                                                   tttACGGTGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTg                              <  2:331904/149‑1 (MQ=255)
                                                                                                                   tttACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGACTCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCCCTACCGCGCGCATCAGCCCCAGGTTg                              >  1:1443532/1‑149 (MQ=255)
                                                                                                                   tttACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTTTGTTGAGACGCGGAAACCACGTTCCGGGTCGACCTTCTCTACCGCGCGGGTCAGCCCCAGGTTg                              >  2:953975/1‑149 (MQ=255)
                                                                                                                   tttACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTg                              <  1:635470/149‑1 (MQ=255)
                                                                                                                   tttACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTg                              <  2:1342009/149‑1 (MQ=255)
                                                                                                                   tttACGATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTg                              >  2:105089/1‑149 (MQ=255)
                                                                                                                        gATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGCTGCCctc                         >  2:1806440/1‑149 (MQ=255)
                                                                                                                        gATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGCGTCAGCCCCAGGTTGCCatc                         >  2:887355/1‑149 (MQ=255)
                                                                                                                        gATGTGAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGGCAAACTTCTCTACCGCGCGGACCAGCCCCAGGTTGCCctc                         >  1:793368/1‑149 (MQ=255)
                                                                                                                          tgtgAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTTCCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCtt                       <  1:945223/149‑1 (MQ=255)
                                                                                                                          tgtgAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAACCCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCtt                       <  1:878086/149‑1 (MQ=255)
                                                                                                                          tgtgAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCtt                       <  1:688813/149‑1 (MQ=255)
                                                                                                                            tgAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCTGATCAGCCCCAGGTTGCCCTCTTCg                     <  1:1178464/149‑1 (MQ=255)
                                                                                                                             gAATCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTTGGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGa                    >  1:154384/1‑149 (MQ=255)
                                                                                                                             gAAGCGGCGAACGGATAGTACGGGTTGGTTCAGAGTCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGGGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGa                    <  2:763121/149‑1 (MQ=255)
                                                                                                                               aTCGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATa                  <  2:707892/149‑1 (MQ=255)
                                                                                                                                 cGGCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAg                <  1:599555/149‑1 (MQ=255)
                                                                                                                                  ggCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGTCCCAGGTTGCCCTCTTCGATAAgg               <  1:1467175/149‑1 (MQ=255)
                                                                                                                                  ggCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGTCCCAGGTTGCCCTCTTCGATAAgg               <  1:1467079/149‑1 (MQ=255)
                                                                                                                                   gCAAACGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAggg              >  2:577914/1‑148 (MQ=255)
                                                                                                                                     aaaCTAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGTGACAACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAAGTTGCCCTCTTCGATAGGGGcc            >  1:1437939/1‑149 (MQ=255)
                                                                                                                                      aaCGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCa           <  2:1544167/149‑1 (MQ=255)
                                                                                                                                       aCGAATAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGAGCAGCCCCAGGTTGCCCTCTTCTATAAGGTCCAg          >  2:783573/1‑149 (MQ=255)
                                                                                                                                       aCGAATAGTACGGGTTGGTCCATACTCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAg          <  1:3564/149‑1 (MQ=255)
                                                                                                                                         aaaTAGTACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGCGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGCATCGGCCCCAGGTGGCCCTCTTCCATAAAGTCAAGCa        >  2:382889/2‑149 (MQ=255)
                                                                                                                                          aaTAGGACGGGGTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCaa       <  2:1651132/149‑1 (MQ=255)
                                                                                                                                               tACGGGTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCCGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCa  >  2:1800747/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
CGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCA  >  CP009273/2860279‑2860570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: