Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,882,644 (T)6→5 coding (1360/1686 nt) fadD ← acyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,882,6390T.96.9% 414.9 / 6.2 97coding (1365/1686 nt)fadDacyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base . (44/50);  total (45/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.26e-01

Only 100 of 102 total aligned reads displayed.

CTTTTTTCACTACGAAGATTTTCACCGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGAC  >  CP009273/1882491‑1882779
                                                                                                                                                     |                                                                                                                                            
cTTTTTTCACTACGAAGATTTTCACCGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTGGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCt                                                                                                                                              >  1:1471641/1‑149 (MQ=255)
     ttCACTACGGAGATTTTCACCGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCttttta                                                                                                                                         <  1:1089502/149‑2 (MQ=255)
       cACTACGAAGATTTTCACCGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATTTCtt                                                                                                                                             >  2:832571/1‑143 (MQ=255)
        accaCGAAGATTTTCACCGCTTCACCCCTGGAGCCGGAAGGTACGCCAACTGCCGCCACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCTGAAACCAGAATCATGTCttt                                                                                                                                            <  2:235195/140‑1 (MQ=255)
             gAAGATTTTCACCGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCtttttacg                                                                                                                                       <  2:1122648/143‑4 (MQ=255)
              aaGATTTTCACCGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACa                                                                                                                               >  1:761091/1‑149 (MQ=255)
               agagTTTCCCCGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGAt                                                                                                                                    <  2:708827/143‑1 (MQ=255)
                gATTTTCCCCGCTTCCCCACCGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAAt                                                                                                                             <  1:2853/149‑1 (MQ=255)
                   tttCACCGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATgcg                                                                                                                          >  1:103491/1‑149 (MQ=255)
                     ctcacGCCTCCCCACCGGAAGCCGGAATGTTCGCTAGCAGCCTCGACTTCCTGTACGCCAGGATGCTGCATTACGACATCTTCAATCTCGTTGGGATAGCCGTTAAAACCGGACACCAGAATCATGTCTTTTTACGATCGACa                                                                                                                               <  2:1206033/139‑1 (MQ=255)
                      cACCGCTTCACCACTGGGGCCGGAAGGTACGCCAACAGGCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAAt                                                                                                                             <  2:1078364/143‑1 (MQ=255)
                      cACCGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGACTCATGTCTGTTTACGATCGACAATGCGCAg                                                                                                                       <  1:1002028/149‑1 (MQ=255)
                      cACCGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAg                                                                                                                       <  1:1795430/149‑1 (MQ=255)
                        ccGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGGAACCAGAATCATGTCTTTTTACGATCGACAATgc                                                                                                                           >  2:1500728/1‑143 (MQ=255)
                         cGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATgcg                                                                                                                          <  2:1512191/143‑1 (MQ=255)
                              caccacTGGAGCCGGAAGGTACGCCAACCGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCctt                                                                                                               <  1:1021703/149‑1 (MQ=255)
                              caccacTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCctt                                                                                                               <  1:1283399/149‑1 (MQ=255)
                              caccacTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCctt                                                                                                               <  1:800994/149‑1 (MQ=255)
                                ccacTGGAGTCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCACGACGACATCTTCAATCCCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCcttct                                                                                                             <  1:403658/149‑1 (MQ=255)
                                ccacTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAAt                                                                                                                   >  2:228022/1‑143 (MQ=255)
                                 cacTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCcttctt                                                                                                            <  1:942341/149‑1 (MQ=255)
                                      gAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCcttct                                                                                                             >  2:387243/1‑143 (MQ=255)
                                         gCCGGAAGGTACGCAAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCttcttc                                                                                                           <  2:251094/143‑1 (MQ=255)
                                          ccGGCAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCa                                                                                                          <  2:74331/143‑1 (MQ=255)
                                          ccGGAAGGTACGCCAACAGGCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCAtt                                                                                                    >  1:1385987/1‑149 (MQ=255)
                                           cGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTa                                                                                                   >  1:327646/1‑149 (MQ=255)
                                             gAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCATTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTAcc                                                                                                 >  1:290378/1‑149 (MQ=255)
                                              aaGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTCGATCGACAATGCGCAGGAATCCTTCTTCATCCAttcctg                                                                                                >  1:647441/1‑145 (MQ=255)
                                               aGGTCCGCCAACACCCGCGACCTCCTGTACGCCAGGATGCTGCATGACGACCTCTTCAACCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACcgc                                                                                               <  1:14265/149‑1 (MQ=255)
                                                  tACGCCCACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGCTGCTCCTTAAAGCCGGTAACCATAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGAt                                                                                            <  1:1398174/149‑1 (MQ=255)
                                                  tACGCCAACAGCCGCGACTTCCTGTACGCCATGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAGTCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGAt                                                                                            <  1:1278671/149‑1 (MQ=255)
                                                     gCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAACCCAGAATCATGTCTTTTTACGAGCGACAATGCTCAGTAAGCCTTCTTCATCCATTACcgc                                                                                               <  2:844010/143‑1 (MQ=255)
                                                     gCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACcgc                                                                                               <  2:1180668/143‑1 (MQ=255)
                                                       caccAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGc                                                                                       <  1:1899668/146‑1 (MQ=255)
                                                        aaCAGCCGCGACTTCCTGGACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGcc                                                                                      <  1:216331/149‑1 (MQ=255)
                                                          cAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCgg                                                                                    <  1:511661/149‑1 (MQ=255)
                                                          cAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCACAGGAATCCTTCTTCATCCATTACCCCGATGTCGCCgg                                                                                    >  1:757661/1‑149 (MQ=255)
                                                           agtcgcgATCTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGgt                                                                                   <  1:748998/146‑1 (MQ=255)
                                                            gccgccACTGCCTGTACGCCAGGTGGCTGCCTGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGCATCCAGACTCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCg                                                                                        <  2:1354607/143‑1 (MQ=255)
                                                            gCCGCGACTTCCTGGACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGgtg                                                                                  <  1:230132/149‑1 (MQ=255)
                                                             ccGCGACTTCCTGTACGCCGGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGgtgt                                                                                 >  1:1385909/1‑149 (MQ=255)
                                                             ccGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGgtgg                                                                                 >  1:538067/1‑148 (MQ=255)
                                                              cgcgACTTCCTGTAAGCCAGGATGCTGCCTGACTTCGTCGGCAATCTCGATGGGATAGACGTTAAAACCGGAAACGAGAATCATGTCTTTTTACGATCGACGATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGgtgtg                                                                                >  1:1408113/1‑149 (MQ=255)
                                                                   cTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACATTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGgtgt                                                                                 <  2:135952/143‑1 (MQ=255)
                                                                    ttCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGCCAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCa                                                                          >  1:659133/1‑149 (MQ=255)
                                                                      ccTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGc                                                                        >  1:1327830/1‑149 (MQ=255)
                                                                        tGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCa                                                                      >  1:879806/1‑149 (MQ=255)
                                                                         gTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAGCCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTGCTTCATCCATTACCGCGATGTCGCCGGTGTGTAAcc                                                                           <  2:1700453/143‑1 (MQ=255)
                                                                          tACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCa                                                                          <  2:106825/143‑1 (MQ=255)
                                                                               cAGGATGCTGCATGACGACTCCCTCCATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTtga                                                               <  1:249434/149‑1 (MQ=255)
                                                                               cAGGATGCTCCATGACGACACCTTCAATCTCGTTGGGATAGAACTTAACGAAAATGACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTtga                                                               <  1:535260/149‑1 (MQ=255)
                                                                                aGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTtgat                                                              <  1:210229/149‑1 (MQ=255)
                                                                                  gATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTACCCAGCCATTTTtgatga                                                            >  1:245768/1‑149 (MQ=255)
                                                                                       tgcATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGACACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAGTCCTTCTTCATCCATTGCCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCa                                                       >  1:41546/1‑149 (MQ=255)
                                                                                         cATGACGACATCTTCAATCTCGTTGGGATATACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATc                                                     >  1:999841/1‑149 (MQ=255)
                                                                                         cATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCAtttttactgaa                                                           >  2:1243381/1‑137 (MQ=255)
                                                                                         cATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATc                                                     >  1:1711513/1‑149 (MQ=255)
                                                                                          aTGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGAtt                                                          >  2:194886/1‑143 (MQ=255)
                                                                                           tGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACAAGCCATTTTTGATGAttt                                                         >  2:323964/1‑143 (MQ=255)
                                                                                               gacATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGAGCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGCTGTCGCCGGTGTGTAACCGGCCCTTTGTACTGATTTCATc                                                     >  2:1415411/1‑143 (MQ=255)
                                                                                                acATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCg                                                    <  2:1605617/143‑1 (MQ=255)
                                                                                                 cATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCAt                                             >  1:1889084/1‑149 (MQ=255)
                                                                                                   tctcCAATCTCGTTGGGATAGACGGTAACACCGAAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAACCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCg                                           <  1:524780/149‑1 (MQ=255)
                                                                                                   tCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTa                                                 >  2:135056/1‑143 (MQ=255)
                                                                                                    cTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCgg                                          >  1:1088345/1‑149 (MQ=255)
                                                                                                     ttCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCACTTTTGATGATTTCATCGGTCGCATCcgg                                         >  1:1641228/1‑149 (MQ=255)
                                                                                                       cAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCAt                                             >  2:1171694/1‑143 (MQ=255)
                                                                                                       cAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCAt                                             >  2:1367794/1‑143 (MQ=255)
                                                                                                         aTCTCGTTGGGATAGCCGTTAAAACCGGAAACCAGACTCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCg                                           <  2:1357348/143‑1 (MQ=255)
                                                                                                         aTCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGc                                     <  1:1735293/149‑1 (MQ=255)
                                                                                                         aTCTCGTTGGGATAGAAGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCg                                           >  2:1070779/1‑143 (MQ=255)
                                                                                                         aTCTCGTTGGCACAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGc                                     <  1:1192757/149‑1 (MQ=255)
                                                                                                          tctcGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCgg                                          <  2:1735766/143‑1 (MQ=255)
                                                                                                             cGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGcc                                 >  1:1234681/1‑149 (MQ=255)
                                                                                                              gTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGCACGCTGccg                                >  1:1638480/1‑148 (MQ=255)
                                                                                                               ttGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGGCGCTGCCAg                               >  1:719781/1‑149 (MQ=255)
                                                                                                               ttGGGATAGACGTTAAAACCGGAAACCAGAAGCATGTCTTTTTACGCTCGCCGATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAg                               <  1:1674267/149‑1 (MQ=255)
                                                                                                                tGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCt                                    >  2:1461205/1‑143 (MQ=255)
                                                                                                                  ggAGAGACGTTAAAACCGAAACCCAGGATCAGGGCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAGCCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGc                                  <  2:424423/143‑1 (MQ=255)
                                                                                                                  caaTAGACGATAAAACCGGAAACCAGAACCATGTCTTTCTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCCATGTCGCCGGTGTGTAACCAGCCATCTTTGATGATTTCATCGGTAGCATCGGGACGCTGc                                  <  2:546091/141‑1 (MQ=255)
                                                                                                                    aTAGACGTTAAAACCGGAAACCGGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCa                                <  2:1565119/143‑1 (MQ=255)
                                                                                                                     tAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAAccc                         <  1:1616908/149‑1 (MQ=255)
                                                                                                                       gACGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAg                       <  1:1846895/149‑1 (MQ=255)
                                                                                                                        aCGTTAAAACCGGAAACCAGAATCCTGTCTTTTGACGATCAACAATGCGCAGGAATCCTGCTTCAGCCATTACCGCGCTGTCGCCGGTGTGTAACCAGCCATTTTTGCTTATTTCATCGGCCGCGTGGGGACGCTgacctgaaccccgg                      >  1:396122/1‑136 (MQ=255)
                                                                                                                        aCGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGc                      <  1:1622212/149‑1 (MQ=255)
                                                                                                                        aCGTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGc                      >  1:1474504/1‑149 (MQ=255)
                                                                                                                         cGTCAAAACCGGAAACCCGAACCAGGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCa                     <  1:278681/149‑1 (MQ=255)
                                                                                                                          gTTAAAACCGGAAACCAGAATCATGTCTTTTTACGATCGACACTGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGCGTGTTAACATCCTTCCTGGCTTATTTCATCGGTAGCATCGGGACGCTGCCAGTACCCCAGCAt                    >  1:364818/1‑149 (MQ=255)
                                                                                                                           tcaaaaCCGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGGGTTGAACCAGCCATTTTTGATGATTTCAGCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATc                   >  1:1130228/3‑149 (MQ=255)
                                                                                                                               aaCCGGAAACCAAAATCCTGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCa                     <  2:799947/143‑1 (MQ=255)
                                                                                                                                 ccGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGCTTTCCTCCGGGGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGc             >  1:356739/1‑149 (MQ=255)
                                                                                                                                  cGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCg            >  1:1713585/1‑149 (MQ=255)
                                                                                                                                  cGGAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCg            <  1:404154/149‑1 (MQ=255)
                                                                                                                                   ggAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTATATTCTTGCTGCGGGACGCTGCCAGTAACCCAGCATCAc                 <  2:802657/143‑1 (MQ=255)
                                                                                                                                   ggAAACCAGAATCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGCTGTGCAACCCGCCATTTGTGAGGATGCCAGCGGTGGCATCGGGACGCTGCCAGTAACCCAGCATCAc                 >  2:190537/1‑143 (MQ=255)
                                                                                                                                     ccccaactaTCACGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCCGTAGCATCGGGACGCTGCTCGTAACCCAGCATCACCt               >  2:1160904/9‑143 (MQ=255)
                                                                                                                                         gacgaTCATGACTTCTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGGCGCCGGTGTGTAGCCAGCCACTTTTAATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCTGTCCttt     <  1:1462520/145‑1 (MQ=255)
                                                                                                                                         cAGAATCATGTCTTTTTACGATCGACAAGGCGCAGGAATCCTTCTTCATCCCTTACCGCGCGGGCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCttt     <  1:920489/149‑1 (MQ=255)
                                                                                                                                           gAATCATGTCTTTTTTCGATCGACTATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGa   <  1:69433/149‑1 (MQ=255)
                                                                                                                                            aaTCATGTCTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGac  >  1:966497/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                            
CTTTTTTCACTACGAAGATTTTCACCGCTTCACCACTGGAGCCGGAAGGTACGCCAACAGCCGCGACTTCCTGTACGCCAGGATGCTGCATGACGACATCTTCAATCTCGTTGGGATAGACGTTAAAACCGGAAACCAGAATCATGTCTTTTTTACGATCGACAATGCGCAGGAATCCTTCTTCATCCATTACCGCGATGTCGCCGGTGTGTAACCAGCCATTTTTGATGATTTCATCGGTAGCATCGGGACGCTGCCAGTAACCCAGCATCACCTGCGGTCCTTTGAC  >  CP009273/1882491‑1882779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: