BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	4,492,563	T→A	H229L (CAC→CTC)	insG ←	IS4 transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,492,563	0	T	A	99.2%	373.7 / NA	118	H229L (CAC→CTC)	insG	IS4 transposase
Reads supporting (aligned to +/- strand): ref base T (1/0); major base A (68/48); minor base C (1/0); total (70/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 118 total aligned reads displayed.

TCAGCAGGCGGGCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGC  >  CP009273/4492417‑4492708

tCAGCAGGCGGGCAGTCACTTCATTTCCCGGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAgg                                                                                                                                                 >  1:1343341/1‑149 (MQ=255)

   gcagGCGGGCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCgg                                                                                                                                              <  1:1187210/149‑1 (MQ=255)

    cagGCGGGCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGgt                                                                                                                                             <  1:23460/149‑1 (MQ=255)

         gggCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCCGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCgg                                                                                                                                              <  2:232934/143‑1 (MQ=255)

         gggCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGGTGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATTCAGAGGCgg                                                                                                                                              >  2:187494/1‑143 (MQ=255)

         gggCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCgg                                                                                                                                              >  2:1621030/1‑143 (MQ=255)

          ggCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGgt                                                                                                                                             <  2:1650219/143‑1 (MQ=255)

          ggCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCAAAGTCACCCTTAGAGGTATCATCCAGAGGCGGTGTtctc                                                                                                                                       <  1:1406967/149‑1 (MQ=255)

            gaGTCACTTCATCTCCCAGTCCCGGCCACTTTTCCCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCTCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTccc                                                                                                                                     <  1:8564/148‑1 (MQ=255)

               tCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATCTTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGcc                                                                                                                                  >  1:868232/1‑149 (MQ=255)

                   ttCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTAGCATCCAGAGGCGGTGTTCTCCCg                                                                                                                                    >  2:883070/1‑143 (MQ=255)

                      aTTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCa                                                                                                                                 <  2:1429114/143‑1 (MQ=255)

                        ttCCCCGTCCCAGCCACTTTTTTCGTGCCTACGGGCTGGTTTTCAGCTTCAGCAGATGATCGCCTTTACCCATTTTTCTGATCCCTTCATATTTCGCTCCCTTTCTGAGAGGTATAATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAg                                                                                                                         <  1:473654/149‑1 (MQ=255)

                        ttCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAg                                                                                                                         <  1:1057594/149‑1 (MQ=255)

                         tCCCAGTCCCGGCACATTTTTTCGTGCCTGCGTGCTGGTTTTCAGCTTCCCCGGGTGATCGCCTTTACCCAGTTTTCTGATCTATTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAgg                                                                                                                        <  1:912460/149‑1 (MQ=255)

                          cccAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGc                                                                                                                       >  1:1845673/1‑149 (MQ=255)

                          cccAGTCCCCGCCACTTTTTTCGTGCCTGCGGGCTGGGTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCt                                                                                                                             <  2:533771/143‑1 (MQ=255)

                            cAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCAt                                                                                                                     >  1:1495950/1‑149 (MQ=255)

                            cAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCAt                                                                                                                     >  1:1011129/1‑149 (MQ=255)

                              gTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAg                                                                                                                         <  2:652741/143‑1 (MQ=255)

                              gTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCAttt                                                                                                                   <  1:1797612/149‑1 (MQ=255)

                               tCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGCTGATCGCCTTTACCCAGTTTCCTGACCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGGTCTCCCGCCAGGCTCCAGGCATTTa                                                                                                                  >  1:1705491/1‑149 (MQ=255)

                                  cGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGCGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTTCAGGCATTTAACa                                                                                                               <  1:1365502/149‑1 (MQ=255)

                                  cGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCAt                                                                                                                     <  2:1666647/143‑1 (MQ=255)

                                  cGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACa                                                                                                               <  1:1633530/149‑1 (MQ=255)

                                      cACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTaa                                                                                                                 <  2:945952/143‑1 (MQ=255)

                                        cTTTTTTCGGGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACa                                                                                                               <  2:862226/143‑1 (MQ=255)

                                         ttttttCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCCGGCTCCAGGCATTTACCAg                                                                                                              >  2:1078908/1‑143 (MQ=255)

                                         ttttttCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCCGGCATTTAACAGTccccg                                                                                                        >  1:370071/1‑147 (MQ=255)

                                         ttttttCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAg                                                                                                              >  2:519303/1‑143 (MQ=255)

                                         ttttttCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAg                                                                                                              >  2:1118405/1‑143 (MQ=255)

                                            tttCGCGCCCCCGGGCTGGTCTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCCGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGa                                                                                                     <  1:165466/149‑1 (MQ=255)

                                                 tGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGtaat                                                                                                >  1:1689864/1‑149 (MQ=255)

                                                     tGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCGCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGCCATTCACCAGTCCCAGTAAGGAATAAcc                                                                                            >  1:1764511/1‑149 (MQ=255)

                                                         ggCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCATTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTa                                                                                        >  1:1173813/1‑149 (MQ=255)

                                                           cTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATc                                                                                      >  1:84312/1‑149 (MQ=255)

                                                            tGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCt                                                                                           >  2:563003/1‑143 (MQ=255)

                                                              ggtttCAGCTTCACCAGATGATCGCCTTTACCCAGCTTCCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCttt                                                                                         <  2:371377/141‑1 (MQ=255)

                                                              gTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCGCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCAt                                                                                   >  1:1404248/1‑149 (MQ=255)

                                                                tttCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGACATTTAACAGTCCCCGTGAGTAATAACCTTTAt                                                                                       >  2:620748/1‑143 (MQ=255)

                                                                 ttCCGCTTCACCAGAGGATCGCCTTTACCCAGTTTTCTGATCCCTTCATATTGCGCTCCCTGTCGGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATc                                                                                      <  2:621569/143‑1 (MQ=255)

                                                                   cAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCa                                                                                    >  2:76305/1‑143 (MQ=255)

                                                                      cTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTACCGTCa                                                                           >  1:1201609/1‑149 (MQ=255)

                                                                      cTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCa                                                                           <  1:1578530/149‑1 (MQ=255)

                                                                      cTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCCGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCa                                                                           >  1:1265523/1‑149 (MQ=255)

                                                                      cTTCACCAGACGATCGCCTTTACCCTGTTTTCTGATCGTTTCATATTGCGCTCCCTCCCAGAGACGGATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCa                                                                           <  1:919278/149‑1 (MQ=255)

                                                                        tCACCAGATGATCGCCTTTACCCAGTCTTCTGATCTCTTCATATTGCGCTTCCTTTCTGGGAGGTATCATCCAGAGGCCGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCaga                                                                         <  1:911934/149‑1 (MQ=255)

                                                                        tCACCAGATGAGCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCaga                                                                         <  1:735840/149‑1 (MQ=255)

                                                                           ccAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCCGGCATTTAACAGTCCCAGTGAGCAATAACCTTTATCCATTAACGTCAGAgtg                                                                      <  1:1158314/149‑1 (MQ=255)

                                                                           ccAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAgtg                                                                      >  1:674592/1‑149 (MQ=255)

                                                                             aGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATTCTTTAACGTCAGAGTGtt                                                                    >  1:812919/1‑149 (MQ=255)

                                                                                    cGCCTTTACCCCGCTTCTCGATCTCTGCCGATTGGCCTCCCTTTCTGAGAGGTCTCATCCAGAGGCGGTGTCCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCg                                                             <  1:617916/149‑1 (MQ=255)

                                                                                        tttACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGc                                                               <  2:1573090/143‑1 (MQ=255)

                                                                                           ccccAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGtt                                                      <  1:769213/148‑1 (MQ=255)

                                                                                           aCCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAGCCTTTATCCATTAACGTCAGAGTGTTATCGCCgg                                                            <  2:968052/143‑1 (MQ=255)

                                                                                             ccAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCCGAGTGTTATCGCCGGtt                                                          >  2:965353/1‑143 (MQ=255)

                                                                                             ccAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCt                                                    >  1:978138/1‑149 (MQ=255)

                                                                                             ccAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAAAGGTATCATCCAGAGGCGGTGTTATCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCt                                                    >  1:1007870/1‑149 (MQ=255)

                                                                                              cAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCta                                                   >  1:1043977/1‑149 (MQ=255)

                                                                                               aGTTTTCTGATCTCTTCATATTGCACTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTg                                                        <  2:1494903/143‑1 (MQ=255)

                                                                                                     cTGATCTCTTCATATCGCGCTCCCCTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGGCATATTGTTATCGCCGGTTTGTTCTATAAGTTg                                            <  1:936989/149‑1 (MQ=255)

                                                                                                      tGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCCTTAACGTCAGAGTGTTATCGCCGGTTTGTTCtata                                                 >  2:933686/1‑143 (MQ=255)

                                                                                                       gATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGATTGTTCTATaa                                                >  2:954449/1‑143 (MQ=255)

                                                                                                        aTCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGATc                                         >  1:1226069/1‑149 (MQ=255)

                                                                                                         tctcTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCTCa                                        <  1:1729595/149‑1 (MQ=255)

                                                                                                         tctcTTCATATTGCGCTCCATTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTGTCCATTAACGACAGAGTGTTATCGCCGGTTTGTTCTATAAGt                                              >  2:809692/1‑143 (MQ=255)

                                                                                                          ctctTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGtt                                             >  2:1623661/1‑143 (MQ=255)

                                                                                                           tctTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGGGGTGTGCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATCAGTTGCTCAGc                                      >  1:459601/1‑149 (MQ=255)

                                                                                                           tctTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCTCAGc                                      >  1:1531067/1‑149 (MQ=255)

                                                                                                            ctTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCTCAGCa                                     <  1:321581/149‑1 (MQ=255)

                                                                                                               cATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCATTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCTCa                                        <  2:1267388/143‑1 (MQ=255)

                                                                                                                   ttGCGCTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGCTTGTTCTATAAGTTTCTCAGCAAGCTCAt                              >  1:607025/1‑149 (MQ=255)

                                                                                                                       gcTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCTCAGCAAGCTCATTTTc                          >  1:1713590/1‑149 (MQ=255)

                                                                                                                       gcTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCTCAGCAAGCTCATTTTc                          >  1:611293/1‑149 (MQ=255)

                                                                                                                        cTCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCACTCAAGGTTTCTATAAGTTGCTCAGCAAGCTCATTTTCg                         <  1:1883463/149‑1 (MQ=255)

                                                                                                                         taacTTTCTGAGAGGTATCATTAAACGGCGGGTTTTACCGGCCAGGCTCCAGGTATTTAACATGCCCAGGGAGTAATAACCTTTATCGATTAACGTCAGAGCGTTATCGCCGGTTTGTTCTATAAGTTGCTCAGCAAGcgccc                              >  2:1021433/4‑139 (MQ=255)

                                                                                                                         tCCCTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCTCAGCAAGCTCATTTTCGc                        <  1:84548/149‑1 (MQ=255)

                                                                                                                         tCCCTTTCTGAGAGGTATCACCCAGAGGCGGTGTTCTCCCGGAACCCTCCAGGCATTTAAACGCCCCTAAAAGGACTAACCGTTAGCCATTCACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCTCAGCAAGCCCAt                              >  2:373553/1‑143 (MQ=255)

                                                                                                                           ccTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCTCAGCAAGCTCAttt                            >  2:263873/1‑143 (MQ=255)

                                                                                                                           ccTTTCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGGTTGTTCTATAAGTTGCTCAGCAAGCTCGttt                            >  2:181389/1‑143 (MQ=255)

                                                                                                                             tttCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCTCAGCAAGCTCATTTTc                          >  2:1376004/1‑143 (MQ=255)

                                                                                                                             tttCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCTCAGCAAGCTCATTTTc                          >  2:1029540/1‑143 (MQ=255)

                                                                                                                              ttCTGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTGGTTCTATAAGTTGCTCAGCAAGCGCATTTTCg                         >  2:12698/1‑143 (MQ=255)

                                                                                                                              ttCTGAGAGGTAGCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCTCAGCAAGCTCATTTTCGCTGttc                   <  1:1130013/149‑1 (MQ=255)

                                                                                                                              ttCTGAGAGCTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATTACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCTCAGCAAGCTCATTTTCg                         <  2:60388/143‑1 (MQ=255)

                                                                                                                                 tGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTGTCGCCGGTTTGTTCTATAAGTTGCTCAGCCAGCCAAGTTTCGCTg                      >  2:586898/1‑143 (MQ=255)

                                                                                                                                 tGAGAGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCTCAGCAAGCTCATTTTCGCTGttcttc                >  1:1038498/1‑149 (MQ=255)

                                                                                                                                  gagaGGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATACCCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCTCAGCAAGCTCATTTTCGCTGt                     >  2:1056292/1‑143 (MQ=255)

                                                                                                                                   agagGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCTCAGCAAGCTCTTTTTCGCTGtt                    >  2:543797/1‑143 (MQ=255)

                                                                                                                                   agagGTATCATCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTCGTTCTATAAGTTGCTCAGCAAGCTCCTTTTCGCTGtt                    >  2:284075/1‑143 (MQ=255)

                                                                                                                                       gTATCAGCCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTg          <  1:568859/149‑1 (MQ=255)

                                                                                                                                          tcatcCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGGTATAGCCGGTTTGTTATATACGTTGCTCAGCAAGCTCATTTTGCCTGTTCTTCagc             >  2:416617/1‑141 (MQ=255)

                                                                                                                                            atcCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGt           >  2:687935/1‑143 (MQ=255)

                                                                                                                                            atcCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGt           >  2:1409372/1‑143 (MQ=255)

                                                                                                                                             tcCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAg    <  1:675553/149‑1 (MQ=255)

                                                                                                                                             tcCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAg    >  1:724496/1‑149 (MQ=255)

                                                                                                                                             tcCAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAg    >  1:1530117/1‑149 (MQ=255)

                                                                                                                                               cAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGc  <  1:768275/149‑1 (MQ=255)

                                                                                                                                               cAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATACATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTGTTAGTTGTTCAGCACGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGc  >  1:255377/1‑149 (MQ=255)

                                                                                                                                               cAGAGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATACATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGc  >  1:1411611/1‑149 (MQ=255)

TCAGCAGGCGGGCAGTCACTTCATTTCCCAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGC  >  CP009273/4492417‑4492708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`