Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,370,282 C→T W166* (TGG→TGA sspA ← stringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,370,2820CT97.3% 223.8 / NA 76W166* (TGG→TGAsspAstringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (32/41);  minor base G (1/1);  total (34/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGATTAACTCCGGCCCAGACGCATTTCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCG  >  CP009273/3370138‑3370423
                                                                                                                                                |                                                                                                                                             
agactacttCCGGCCGAGATGCATTTCACGTTCTGCTTCAGTTTAAGAAGCAAGGAACGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCGCTGTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTcaca                                                                                                                                           <  1:563935/141‑1 (MQ=255)
 gATTAACTCCGGCCCAGACGCATTTCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCAcag                                                                                                                                          >  1:372759/1‑149 (MQ=255)
  aTTAACTCCGGCCCAGACGCATTTCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGGCGACGTCAcagc                                                                                                                                         >  2:844155/1‑149 (MQ=255)
  aTTAACTCCGGCCCAGACGCATTTCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCAcagc                                                                                                                                         <  2:904131/149‑1 (MQ=255)
      aCTCCGGCCCAGACGCATTTCACGTTCTGCTTCAGTTAAAGACGCAAGGAAAGCGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCg                                                                                                                                     <  1:797763/149‑1 (MQ=255)
       cTCCGGCCCAGACGCATTTCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCgg                                                                                                                                    <  1:360903/149‑1 (MQ=255)
        tCCGGCCCATACGCATTTCACCTCCCGCACCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGa                                                                                                                                   <  2:49393/149‑1 (MQ=255)
        tCCGGCCCAGACGCATTTCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGa                                                                                                                                   <  1:315178/149‑1 (MQ=255)
        tCCGGCACAGAGGCATTTCACGTTCTGCTTAAGTTATAGAAGCAAGGAAAGAGTAACGCTCAAAGACGCGGGTGCTAAAGCCATTCAGCTCTTTCGCACCCGGGCCGCTGCACAAGATGCCCAGTTGAGGAAGACGGCACGACAGCgca                                                                                                                                   >  2:422059/1‑147 (MQ=255)
              ccAGACGCATTTCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGa                                                                                                                             >  1:881018/1‑149 (MQ=255)
               cAGACGCATTCCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGAt                                                                                                                            <  1:209124/149‑1 (MQ=255)
                           aCGTTCTGCTTCAGTTAAAGGAGCATGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCTGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCa                                                                                                                <  1:579516/149‑1 (MQ=255)
                             gTTCTGCTTCAGTTAAAGAAGCGAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATTGCCTTTCAGCTCTTTCGCACCGGGGCCGCTGCACTCGATGCCCAGTTGCGGCAGACGTCACGGCAGCGGAGCAATATAGCAATCGACCAgg                                                                                                              <  1:604163/149‑1 (MQ=255)
                                  gCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGCCGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGaa                                                                                                         <  2:765415/149‑1 (MQ=255)
                                  gCTTCAGTTAAAGAAGCAAGCAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGaa                                                                                                         <  1:842815/149‑1 (MQ=255)
                                   cTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAAc                                                                                                        >  1:821638/1‑149 (MQ=255)
                                    ttCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACt                                                                                                       >  1:170479/1‑149 (MQ=255)
                                         ttAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCg                                                                                                  >  1:889477/1‑149 (MQ=255)
                                         ttAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCg                                                                                                  >  2:642267/1‑149 (MQ=255)
                                         ttAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGAGCCGCTGAACCCGATCCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCCAGACAGCAATCGACCAGGCCGAACCCATCg                                                                                                  >  2:270062/1‑149 (MQ=255)
                                         tcaaaGAAGAAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATAGAGCCTGTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCg                                                                                                  <  1:69306/147‑1 (MQ=255)
                                            cagaagCAATGAAAGAGCCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGCTGCTGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTc                                                                                               <  1:422059/148‑1 (MQ=255)
                                                  cAAGGACAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAg                                                                                         >  2:114718/1‑149 (MQ=255)
                                                  cAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGGTGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCACGATAGCAATCGGCCGGGCTGAACTCATCGCTCAGGAAg                                                                                         >  1:45393/1‑149 (MQ=255)
                                                  cAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAg                                                                                         >  2:114740/1‑149 (MQ=255)
                                                  cAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCACTAACTCAGCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAg                                                                                         >  1:658722/1‑149 (MQ=255)
                                                     ggAAAGAGGCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTAc                                                                                      <  1:266312/149‑1 (MQ=255)
                                                      gAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCACTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCCGGCTGAACTCATCGCTCAGGAAGTACg                                                                                     >  2:342237/1‑149 (MQ=255)
                                                       aaaGCGGCACGCTCAAAGACGCGGGTCATATATCCTTTCAGCTCTTTCGCAGCCTGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACTAGGCTGAACTCACCGCTCAGGAAGTACgg                                                                                    <  1:600909/149‑1 (MQ=255)
                                                        aaGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGc                                                                                   <  1:76066/149‑1 (MQ=255)
                                                               actcTCAAAGCCGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCACTGAACTCGATGCCCAGTTGCGGCAAACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGCACGGCTTCTGAc                                                                            <  1:688154/146‑1 (MQ=255)
                                                                   tCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACCCATCGCTCAGGAAGTACGGCTTCTGACCGaa                                                                        >  1:243992/1‑149 (MQ=255)
                                                                    cAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAg                                                                       >  1:194282/1‑149 (MQ=255)
                                                                     aaaGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGACCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGa                                                                      >  1:226973/1‑149 (MQ=255)
                                                                          cgcgGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGgc                                                                 <  2:221485/149‑1 (MQ=255)
                                                                          cgcgGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAAACGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGgc                                                                 <  1:424130/149‑1 (MQ=255)
                                                                           gcgTGTCATATAGCCTTTCATCTCTGTCGCACCCGCGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGTAGCAAGATAGCAATCGACCAGGCGGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGgcg                                                                <  2:571670/149‑1 (MQ=255)
                                                                           gcgGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGgcg                                                                >  2:383427/1‑149 (MQ=255)
                                                                             gggTCATATAGCCTTTCAGCTCTTTCGAACCGGGGCCGCTGAACTCGATGCCCAGTTGCGACAGACGACACAGCAGCGGACCAAGATAGCAATCGCGCAGGCTGAACCCATAGCTCAGGGAGTAAGGCTTCTGACCGCAGACCgggccg                                                              >  2:323873/1‑145 (MQ=255)
                                                                               gTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAAt                                                            >  1:448993/1‑149 (MQ=255)
                                                                                tCATATAGCCTTTCCGCTCTTTCGCCCCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATc                                                           <  2:661152/149‑1 (MQ=255)
                                                                                 cATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCg                                                          <  1:170100/149‑1 (MQ=255)
                                                                                     taGCCTTTCAGCTCTTGCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCcag                                                      <  1:99160/149‑1 (MQ=255)
                                                                                      aGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCcagc                                                     >  2:637011/1‑149 (MQ=255)
                                                                                         cTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGGACTCCTCGTTCGGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGt                                                  >  1:73759/1‑149 (MQ=255)
                                                                                            ccAGCTCGTCCCCACCCGGGCCGCTCAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCCGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGttct                                               <  1:721890/148‑1 (MQ=255)
                                                                                             cAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGttctt                                              <  1:107756/149‑1 (MQ=255)
                                                                                              aGCTCTTTCGCACCCGGGCCGCTGACCTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCTAATACCGGCGCAATCGCCAGCAGttcttc                                             <  2:923565/149‑1 (MQ=255)
                                                                                              aGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGttcttc                                             <  2:307296/149‑1 (MQ=255)
                                                                                              aGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGAGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTGCTGACCGAAAACCGGCGCAACCGCGCGCAGttcttc                                             >  2:706532/1‑149 (MQ=255)
                                                                                                 tctTTCGCCCCCGGGCCGCTGAACTCGATGCTAAAGCGCTTCATACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGAACGGCTTCTGAACATAGAACGGAGCAACCGTAAGCAGTTCTTcgcg                                          <  2:671919/149‑1 (MQ=255)
                                                                                                 tccttcccccccGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTcgcg                                          <  1:26089/140‑1 (MQ=255)
                                                                                                       gCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAAGCGCCAGCAGTTCTTCGCGCAGTTg                                    >  2:619294/1‑149 (MQ=255)
                                                                                                         ccccGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGACAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCt                                  <  1:313053/148‑1 (MQ=255)
                                                                                                         aCCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCCGTTCTTCCCGCAGTTGCt                                  >  2:192611/1‑149 (MQ=255)
                                                                                                         aCCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCt                                  >  1:768304/1‑149 (MQ=255)
                                                                                                          cccGGGCCGCTGAACTCGATGCCCAGTCGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCtt                                 <  1:371626/149‑1 (MQ=255)
                                                                                                            cGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGCCCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGGGCAGtagactcc                               >  2:647484/1‑142 (MQ=255)
                                                                                                              ggCCGCTGAACTCGACGCGCCGTTGCGGCTGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCACCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGt                             <  1:18972/149‑1 (MQ=255)
                                                                                                                  gCTGACCTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCgg                         <  1:417032/149‑1 (MQ=255)
                                                                                                                  gCTGAACTCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCCGTTCTTCGCGCAGTTGCTTACGTGCgg                         >  1:112896/1‑149 (MQ=255)
                                                                                                                         tccaTGCCCAGGTGCGGCAGCAGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTCCGGCTTCTGACCGAAGACCGGCGCAAACGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGc                  <  1:64834/146‑1 (MQ=255)
                                                                                                                         tCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGc                  <  1:114740/149‑1 (MQ=255)
                                                                                                                         tCGATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGc                  <  1:114718/149‑1 (MQ=255)
                                                                                                                           gATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCtt                <  2:821638/149‑1 (MQ=255)
                                                                                                                           gATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCtt                <  2:860703/149‑1 (MQ=255)
                                                                                                                           gATGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGGTCTTCGCGCGATTGCTTACGTGCGGCGTCTGcct                >  1:787111/1‑147 (MQ=255)
                                                                                                                            aTGCCCAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTc               >  1:835402/1‑149 (MQ=255)
                                                                                                                            aTGCCCAGTTGCGGCAGACGGCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTc               <  1:534530/149‑1 (MQ=255)
                                                                                                                                ccAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGaa           >  1:397573/1‑149 (MQ=255)
                                                                                                                                 cAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAg          <  2:37106/149‑1 (MQ=255)
                                                                                                                                 cAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAg          >  1:356813/1‑149 (MQ=255)
                                                                                                                                 cAGTTGCGGCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAg          <  1:723816/149‑1 (MQ=255)
                                                                                                                                       cGGCTGACGTAACAGCAGCCGAGCAAGATACCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAAc    <  2:187767/149‑1 (MQ=255)
                                                                                                                                        ggCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAAcc   >  1:605984/1‑149 (MQ=255)
                                                                                                                                         gCAGACGTCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCg  >  1:38766/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                             
AGATTAACTCCGGCCCAGACGCATTTCACGTTCTGCTTCAGTTAAAGAAGCAAGGAAAGAGTCACGCTCAAAGACGCGGGTCATATAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCATCTGCTTCAGAAGCTGAACCG  >  CP009273/3370138‑3370423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: