Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
JC | CP009273 | 3,270,632 | (TTCAACA)2→3 | coding (272/336 nt) | prlF → | antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system |
New junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | CP009273 | = 3270616 | 1 (0.020) | 38 (0.850) +CATTCAA |
35/250 | 0.2 | 97.4% | coding (256/336 nt) | prlF | antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system |
? | CP009273 | 3270617 = | 1 (0.020) | coding (257/336 nt) | prlF | antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system |
CCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTAC > CP009273/3270469‑3270761 | aggacagcattcattacgaaattctgcctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactctcccactcaac > 1:305024‑M2/149‑149 (MQ=255) acagcattcattacgaaattctgcctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaacatt < 2:749331‑M2/4‑1 (MQ=255) agcattcattacgaaattctgcctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaacattca < 2:261600‑M2/6‑1 (MQ=255) attcattacgaaattctgcctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaaattcaaca > 2:594273‑M2/141‑149 (MQ=255) gaaattctgcctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCAGTCAACATTCAA‑‑‑‑‑‑‑CAAg > 1:723984‑M2/132‑149 (MQ=255) gaaattctgcctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaaaaacccgcaaaaaactcgtccagtccaCGTACAACATTCAA‑‑‑‑‑‑‑CAAg > 1:313532‑M2/132‑149 (MQ=255) aattctgcctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccgttcaaCATTCAACATTAAA‑‑‑‑‑‑‑CAAGga > 1:212284‑M2/130‑149 (MQ=255) aattctgcctgctgggcagggatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGga < 2:67939‑M2/20‑1 (MQ=255) attctgcctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGgaa > 2:500335‑M2/129‑149 (MQ=255) ctgcctggtgggcaagtatttatgtgaccactggaagacgaacaagaagatcatattatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGgaaaga < 2:335299‑M2/24‑1 (MQ=255) tgcctggtgggcaagtatttatgtgccgactgggggatgaacaggaggatcatactatgaatgcctttttgcgttttctggatgcagatatccagaacaccccgcaaaaaactcggccattcaaCATTCAACATTGCA‑‑‑‑‑‑‑CAAGgaaagaa > 2:145660‑M2/125‑149 (MQ=255) ctggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACt > 1:774705‑M2/122‑149 (MQ=255) tggtgggcaagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACtt < 2:673857‑M2/29‑1 (MQ=255) caagtatttatgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTg > 2:391767‑M2/114‑149 (MQ=255) tgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAC‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGt > 2:190418‑M2/104‑149 (MQ=255) tgtgccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccaaccaaCATTCAACAATAAA‑‑‑‑‑‑‑CAAAGAAAGAAAATAATAACTAGCATGGACGt > 2:162874‑M2/104‑149 (MQ=255) gccgactgggagatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCaa > 1:537662‑M2/101‑149 (MQ=255) cgtctgggagatgaacagggggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACa < 1:148678‑M2/51‑1 (MQ=255) cgtagatgaacaacaggatcatactaacaatgcatttttccattttctggatgcagatatccagaacaacccgcaaaaaaatcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATtgat < 2:66077‑M2/56‑1 (MQ=255) agatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACCTtgatgat > 2:307361‑M2/91‑149 (MQ=255) gatgaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATtgatgatg > 2:709399‑M2/90‑149 (MQ=255) gaacaggaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAAGATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGACATGGACGTCAACATTGATGATgaga > 2:407482‑M2/87‑149 (MQ=255) gaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGcg > 2:58700‑M2/81‑149 (MQ=255) gaggatcatactatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGcg > 2:625642‑M2/81‑149 (MQ=255) atactatgaatgcatttttgcgttttctggatgcagacatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTTGCATGGACGTCAACATTGATGATGAGATTGGcgacgacga < 1:368097‑M2/76‑1 (MQ=255) ctatgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGaata < 1:625642‑M2/79‑1 (MQ=255) atgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaacctcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATAAGATTGACGACGACAaataat > 2:249287‑M2/69‑149 (MQ=255) atgaatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAAATTGGCGACGACGaataat > 2:83280‑M2/69‑149 (MQ=255) aatgcatttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGa < 2:313532‑M2/84‑1 (MQ=255) catttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGAtttt < 1:678976‑M2/88‑1 (MQ=255) catttttgcgttttctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGAtttt < 2:774705‑M2/88‑1 (MQ=255) ttttgcgttttctggatgcagatatccagaacaacccgcaaaaagctcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGACATGGACGACAACATTGATGATGAGATTGGCGCCGACGAATAATGGACTTTCca > 1:406020‑M2/59‑149 (MQ=255) ctggatgcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTaa > 1:123539‑M2/48‑149 (MQ=255) atgcagatatccagaacaacccgcaaaaaactcgaccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAGGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAGTAATGGATTTTCCACAAAGGGTTAATGGt > 1:544306‑M2/44‑149 (MQ=255) agtgatatatagagaacaacccggaaaagactcgtccattcaaCATTCAATATTGAG‑‑‑‑‑‑‑CAAGGAAAGAAACTGGTCGCTGGCATGGACGTCAACATTGAGGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGt < 1:664456‑M2/106‑1 (MQ=255) gcagatatccagaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAAAAATGGATTTTCCACAAAGGGTTAATGGTTg > 2:28435‑M2/42‑149 (MQ=255) aagaagctatCCAGGACAACCCGCAAAAAACTCGTCCATTCAACATTCAACATTCACCAAGGAAGGAAACTTGTCGCTGGCCTGGACGTCAGCATTGATGATGGGGTTGGCCACGACGAATAAGGGATTGTCCGCAGAAGGATAATGGt > 1:133973/8‑149 (MQ=255) agaacaacccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTatat > 2:750408‑M2/32‑149 (MQ=255) acccgcaaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCAt < 2:363452‑M2/124‑1 (MQ=255) aaaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGt < 2:427881‑M2/130‑1 (MQ=255) aaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGtt > 1:661444‑M2/19‑149 (MQ=255) aaaaactcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAGTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGtt < 2:380552‑M2/131‑1 (MQ=255) actcttccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCa < 1:500335‑M2/135‑1 (MQ=255) ctcgtccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAg < 1:524387‑M2/136‑1 (MQ=255) tcgtccattcaaCATTCAACATTAAA‑‑‑‑‑‑‑GAATGAAAGAAACTTGCCGCTGGCATGGACGTCAACATTGAGGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAgg < 2:257343‑M2/137‑1 (MQ=255) tccattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGaaa < 2:646004‑M2/140‑1 (MQ=255) cattcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGGGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAAcc > 2:88385‑M2/8‑149 (MQ=255) tcaaCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTac > 2:75555‑M2/5‑149 (MQ=255) | CCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTATGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAA‑‑‑‑‑‑‑CAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTAC > CP009273/3270469‑3270761 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
Reads not counted as support for junction |
read_name Not counted due to insufficient overlap past the breakpoint. |
read_name Not counted due to not crossing MOB target site duplication. |