| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009273 | 3,960,035 | C→A | R87S (CGC→AGC) | rho → | transcription termination factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,960,035 | 0 | C | A | 98.2% | 166.2 / NA | 57 | R87S (CGC→AGC) | rho | transcription termination factor |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (28/28); minor base G (0/1); total (28/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAG > CP009273/3959889‑3960178 | tcttGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATcagc < 2:245238/146‑1 (MQ=255)tCCTGAAGCAGTACGAAAAGAGTGGCGAAGATATCTTGGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATcagc < 2:245523/149‑1 (MQ=255) agcagcACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTc < 1:738918/149‑1 (MQ=255) aCGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGCATAGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTc > 2:605236/1‑149 (MQ=255) ggTGGCGAAGATATCTTTGGTGCTGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTgg < 1:173319/148‑1 (MQ=255) ttGGCGAGCATATCTTGGTTGATGGCGTACGGGAGATATTGCCGGAAGAAGTGGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCGAATCAGCCGTTTCAACCTCCGCACTGGt < 2:177508/148‑1 (MQ=255) ggCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGa < 2:598850/149‑1 (MQ=255) gCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGGCGGTTCAACCTCCGCACTGGTGag > 1:419204/1‑148 (MQ=255) gCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAAACAGCTCCAACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGAt > 2:214046/1‑149 (MQ=255) gAAGGTTTCTTTGGTGGTGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGTGCTCTCAGCCGTTTCAACCTCCGCACTGGTGATAc < 1:168392/149‑1 (MQ=255) aaGAGATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATAcc < 2:229446/149‑1 (MQ=255) atatCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCACCGCACTGGTGATACCAtc > 2:677606/1‑149 (MQ=255) ggTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCTCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGTACTGGTGATACCATCTCTGGTaa < 2:407501/149‑1 (MQ=255) tgtatgggcgaCTGGAGGTATTGCAGGATGGATTTTGTTTTCTCGTTTCCGCAGACAGCTCCTACCTCTTCGGTCCTGATGACCTCTACGTTTCTCCTAGCTAAATCATCCGTTTCAACCTCCGCACTGGTGATACCCTCTTTGGTAAg < 1:9400/139‑1 (MQ=255) ggCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCACCCTCCGCCCTGGTGATACCATCTCTGGTAAGATTcg < 1:721476/149‑1 (MQ=255) aCTGGAGATATTTCAGGATGGATTTTGTTTCCACCGTTCCGCATATAGCTCCTACCTCGCCGGTCCTAATGACAACTACGCTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTcgcccgc < 2:245471/149‑1 (MQ=255) cTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTcgcccgcc > 1:55981/1‑149 (MQ=255) agaTATTGCAGGATGGATTTGGTTTCCTCCGCTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCTAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGaaa < 2:520701/149‑1 (MQ=255) tGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAGGCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGt < 2:340236/149‑1 (MQ=255) tGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCCCTGGTAAGATTCGCCCGCCGCAAGAAGGt > 1:260226/1‑149 (MQ=255) gCAGGATGGATTTGGTTTCCTCCGTTGCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTg > 1:767416/1‑149 (MQ=255) gCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTg > 2:73461/1‑149 (MQ=255) cAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGATGa > 2:104936/1‑149 (MQ=255) tgatTTGGTGTCTCCCGTGCCTCAGACAGCTCGTCCCTCGCCGGTCCTCATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTa < 1:592229/148‑1 (MQ=255) gatTTGGTTTTCTCCGTGCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACATTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTAt > 1:573187/1‑149 (MQ=255) atTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCCCCCGCCGAAAGAAGGTGAACGCTAtt > 1:398738/1‑149 (MQ=255) atTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGCTTCCCCTAGCCAAATCAGACGTTTCAACCTCCACACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTAtt > 2:86981/1‑149 (MQ=255) gTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGCTACCATCCCTGGTAAGATTCGCCCGCCGAAAGAGGGGGCACGCCATTTTccg > 1:12183/1‑149 (MQ=255) gTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGCGAACGCTATTTTgcg > 1:121944/1‑149 (MQ=255) tttCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGACGGTGAACGCTATTTTgcgc > 1:135786/1‑149 (MQ=255) tttCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGATGATACCATCTCTGGTAAGATTCGCCCGCGGAAAGAAGGTGAACGCTATTTTgcgc > 2:461324/1‑149 (MQ=255) tcctccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCgctg < 2:546381/149‑1 (MQ=255) ctccGTTCCGCAGACAGCTCCTACATCGCCGGTCCTGATGACATCTACGTTTCCACTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTTCTTTGCgctgct > 1:176288/1‑149 (MQ=255) tccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGctgctg > 2:593124/1‑149 (MQ=255) gtgccGCAGGCAGCGCCGACCGCGCGGGTCCTGATGAGCTCTACGTTGCCCCTAGACAGATCGGCGGTTTCAGCCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCGCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGaaa < 1:30946/146‑1 (MQ=255) gTTCCGCAGCCAGCTCCTCCCTCGCCGGTCCTGATGACATTTACGTGTCCCTTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGaaa < 2:405459/149‑1 (MQ=255) gTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGaaa < 2:190387/149‑1 (MQ=255) ccGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGtt > 1:294911/1‑149 (MQ=255) gccagacatacccTACCTCGCCGGTCCTGATGACATCTACGCTTCCCCGAGTCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTTTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTa < 1:13014/138‑1 (MQ=255) cGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCCCCGCCCTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAAGTGAACGCTATTTTACGCTGCTGAAAGTTa > 2:10692/1‑149 (MQ=255) cTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGCTTCGCCCGCGGCTAGCTGGTGTAGGCTTTTTTGCGCTGCTGAAAGTAAAGGAAGTTa > 2:234721/1‑149 (MQ=255) tCCTACCTCGCCGGTCCTGATGACAGCTACGTTTCCCCTAGCCACATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCACTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTaa < 2:478112/149‑1 (MQ=255) ccTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGGTTCAACCTCCGCACGGGTGATACCATATCTGGAAAGAGTCGCACGACGAAAGAATTTGAACGCTATTTTGCGCTGCTAAAAGTTAACGAAGTTAAc < 2:568499/149‑1 (MQ=255) cTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCACCTTCCCCACCAGAGATACCATCACTGGTAAGATTCGCCCCCCCAAAGAACATTAACACTATTTCGCGCTACTTAAAAGTAACGACATTAACt > 2:451084/1‑149 (MQ=255) tACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTCCGCCCCTGAACGTTAACGAAGTTAACtc > 2:306156/1‑148 (MQ=255) aCCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTc < 2:55981/149‑1 (MQ=255) ccTCGCCGGTCCTGATGACTTCTACTTTTCCCCTAGCCATATCAGCTGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAActtcgttaactttc < 2:279265/149‑14 (MQ=255) tcgtcgGTCCTGTGTGCATCGACGTTTCCGCTAGCCAAACCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGAc < 1:271107/149‑1 (MQ=255) gaCTTCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGc < 2:661972/149‑1 (MQ=255) aCATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGcc < 2:213713/149‑1 (MQ=255) agcTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCGCCGCACTGGTGATACCATCTCTCGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTACCGCAGTTAACTTCGACAAACCTGAAAACGCCCg < 2:378896/147‑1 (MQ=255) aTCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCGGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCg > 1:716102/1‑149 (MQ=255) tCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGc > 1:709460/1‑149 (MQ=255) tttCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCAACTCAGGTACGATCCGCCCGCCGAAAGAAGGTGCACGCTATTATGCGCTGCTGAAAGTTAACGCAGTTAACTCCGGAAAACCTGAAGACGCGCGcaggcaa > 1:601017/1‑144 (MQ=255) ttCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACGGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCAGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACaaaa < 2:532195/149‑1 (MQ=255) cTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGCTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCctc > 1:595761/1‑149 (MQ=255) aGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCAGCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCtt < 1:686864/149‑1 (MQ=255) gCCAAATCAGCCGGTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCttt < 2:317978/149‑1 (MQ=255) aaaTCAGCCGTTTCAACCTCCGCACTGGTGATACCCTCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCAGAAAACGCCCACAACAAAATCCTCTTTgag > 2:12236/1‑149 (MQ=255) | TCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAG > CP009273/3959889‑3960178 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |