| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,219,839 | C→G | 53.9% | R83G (CGT→GGT) | ygjI → | putative transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,219,839 | 0 | C | G | 53.9% | 0.9 / 11.0 | 13 | R83G (CGT→GGT) | ygjI | putative transporter |
| Reads supporting (aligned to +/- strand): ref base C (4/2); new base G (3/4); total (7/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTAT > CP009273/3219695‑3219957 | cTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAGTAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTg > 1:52411/1‑149 (MQ=255) tCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAATATGGCTGGGCGGAGGTTGGCCAtgt > 2:284031/1‑142 (MQ=255) ccccTCCTTTCTAATGATCGAACAATTTGTTCCGTTAAAAAAAACCCCAGAAGCCGCTATTTGCGCGGTGTTAAAAAGTTCCCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCt < 1:191131/148‑1 (MQ=255) ccTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCa < 2:19308/144‑1 (MQ=255) ccTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCa > 2:328768/1‑144 (MQ=255) aGAATTTGTTTCGTTAAATAAACACGCAGGAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTGCGTAAACCTGTCCTTTTTCACCTCACTGTTGCCGCGCGTTATTg < 1:110309/149‑1 (MQ=255) aaaTTTGTTTCGTTAAATAAAAACCCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGc < 1:123685/148‑1 (MQ=255) aaTTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCt > 1:215418/1‑149 (MQ=255) ttCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTa < 2:163882/144‑1 (MQ=255) aGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGAt > 1:368873/1‑149 (MQ=255) gAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGCACGTTGGGCATTTATTACTGCCTATCCCTACTGGTTCGTAAACCTGTTCTTTTTCGCCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATACCTCCCtg < 2:218535/144‑2 (MQ=255) aaGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGACTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGAtcc > 1:55583/1‑147 (MQ=255) gTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATGCGacagttgttt > 2:65997/1‑135 (MQ=255) | CTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTAT > CP009273/3219695‑3219957 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |