| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 628,963 | C→G | 40.8% | intergenic (‑30/‑79) | ybdH ← / → ybdL | putative oxidoreductase/methionine aminotransferase, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 628,963 | 0 | C | G | 40.8% | 10.6 / 30.1 | 27 | intergenic (‑30/‑79) | ybdH/ybdL | putative oxidoreductase/methionine aminotransferase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (8/8); new base G (6/5); total (14/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01 | |||||||||||
GCGCGAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCACCC > CP009273/628824‑629105 | gcgcgAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCt < 2:264094/144‑1 (MQ=255) gagaATGTTGTTCATCAGTGAAAACATCGTGCAGGTGATTGAAACTTTCTGGATGTGAAAAGTAGTTCGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGAGATCTAAAc < 2:354292/144‑1 (MQ=255) gagaAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGGTTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGACCGACGACCACGCGGATAGGAGTGTAAGGCCCGGCGTGTGGCCCATATTGATTTCTTTAGACATCTAAAc > 2:159864/1‑144 (MQ=255) gagaAAGTTGTTCATAAAAAAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGAGATCTAAAc < 2:196139/144‑1 (MQ=255) gagaAACTTGGTCATCAGCGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGTCATGTTGTGTTGTCCTTATTGTTTTATTTAGAGATCTAAACGTCtt < 1:162008/149‑1 (MQ=255) aaGTTGTTAATAAGTGAAAAAATCGTGCAGGTGATTGAACTTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGAtt < 1:123020/149‑1 (MQ=255) tcaGTGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGAGATCTAAACGTCTTGATTGCCAAATACt > 1:187276/1‑149 (MQ=255) attGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGAGATCTAAACGTCTTGATTGCCAAATACTAg < 1:298464/147‑1 (MQ=255) aaaaaTCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCg > 1:6230/1‑149 (MQ=255) tGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGAGATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGccgt < 1:331571/149‑3 (MQ=255) gATTGAAACTTCCTGGACGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTc < 1:85169/149‑1 (MQ=255) aTTGAAACTTCCTGGATGAGAAAAGTAGTTAGCCGGGCGGACGACCACGCGGAAAGGATTGTGAGGCATGTTGTGGTGTCCTTATTGTTTTATTTAAAGAGCGAAACGTCTTGATTGCCAAATACTAGCATCGTGGTATTGTGCCttaa > 1:49896/1‑147 (MQ=255) aaaCTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAacac > 1:331904/1‑149 (MQ=255) ccGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGa < 1:275975/149‑1 (MQ=255) ggCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACaa > 1:228064/1‑149 (MQ=255) aTTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAAATTCGTCAGGTACAAAAAAAATGACAAATAACCCTCTGATTAca > 2:289318/1‑144 (MQ=255) ttGTGAGGCATGTTGTGTTGTCCTTATTGATTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGc > 1:242656/1‑149 (MQ=255) aGGCATGTTGTGTTGTCCTTATTGATTTATTTAGAGATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACt > 1:296626/1‑149 (MQ=255) cATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTcc < 1:54513/149‑1 (MQ=255) ttgtCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGCTTCCACAAAGCAAACTTCCACaa > 2:349426/1‑144 (MQ=255) gtCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCAACAAAGCAAACTTCCCCAACTTGGca > 1:163247/1‑149 (MQ=255) tCCTTATTGTTTTATTTAGAGATCTAAACGTCTTGATTGCCAAATACTAGCATAGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACtt > 2:237733/1‑144 (MQ=255) cTTATTGTTTGATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTgg < 2:337678/144‑1 (MQ=255) tATTGTTTTATTTAGAGATCTAAACGTCTGGATTGCCAAATACTAGCATCGTGATATAGTGCCGTCAACACGCAACTTCGTCAGGTACAATAAAGACGACAAATAACACTCTGATTACACAAAGCAAACTTCCACAACTTGGca > 2:192799/1‑144 (MQ=255) ttGTTTTATTTAGAGATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCGTCAACACGCAACTTCGTCAGGTACAATAAACATGACAAATAACCCTCCGATTCCACAAAGCACACTTCAACACCTTGGCACAACTAt > 1:53532/1‑149 (MQ=255) ttttATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTAtttt < 1:312678/149‑1 (MQ=255) aCATCTAAACGTCTTGATTGCCAAATACGAGCCTCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCAccc < 2:5411/144‑1 (MQ=255) | GCGCGAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCACCC > CP009273/628824‑629105 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |