| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,219,839 | C→G | 42.6% | R83G (CGT→GGT) | ygjI → | putative transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,219,839 | 0 | C | G | 42.6% | ‑0.1 / 13.4 | 14 | R83G (CGT→GGT) | ygjI | putative transporter |
| Reads supporting (aligned to +/- strand): ref base C (4/4); new base G (2/4); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01 | |||||||||||
GCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCAT > CP009273/3219694‑3219978 | gCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTg > 2:332571/1‑150 (MQ=255) ttCCTCGCGACGTTTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTgg < 1:116177/150‑1 (MQ=255) tCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCg < 2:540/150‑1 (MQ=255) gcgACGATTTTTTATTTTATTCCCTTCTGGCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTa < 2:289312/150‑1 (MQ=255) ttattCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTGCTGGTTGGGAAACCTGTTCTTTTTCa > 1:308103/1‑150 (MQ=255) gAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCt < 1:332571/150‑1 (MQ=255) gTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGGAGAGGTCGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCt < 2:352310/150‑1 (MQ=255) gTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCCTa > 1:217596/1‑150 (MQ=255) gggcgcgGGGGTAAAAAGTTCGCTGGGCGGACGTTGGGGATTTATTACTGCCTATACCTACTGGTTCGTAAGCCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATAtattat < 2:13517/147‑1 (MQ=255) aaaaaGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGGAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGc > 2:337665/1‑150 (MQ=255) aaaGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCGTCCTCGGATACGAATATATTATGACGCCGGTTGcca > 1:234139/1‑150 (MQ=255) aaaGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGcca < 2:94555/150‑1 (MQ=255) aGTTCGCTGGGCGGAGGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGccacc < 2:308103/150‑1 (MQ=255) gCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGCTACGAATATATTATGCCGCCGGTTGccaccaccag > 2:71560/1‑149 (MQ=255) | GCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCAT > CP009273/3219694‑3219978 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |