| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,277,164 | A→G | 13.9% | L88P (CTG→CCG) | yejK ← | spermidine nucleoid‑associated protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,277,164 | 0 | A | G | 13.9% | 72.8 / 4.6 | 33 | L88P (CTG→CCG) | yejK | spermidine nucleoid‑associated protein |
| Reads supporting (aligned to +/- strand): ref base A (11/17); new base G (2/3); total (13/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.84e-01 | |||||||||||
TGGGTTGATGTCGAGATTTTCGTTAACGCGCATACTGCTCAGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGACCCGATGCAGTTCTGCCACCATC > CP009273/2277016‑2277313 | tgggttgATGTCGAGATTTTCGTTAACGCGCATACTGCTCAGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAg > 2:58621/1‑150 (MQ=255) tGTCGAGATTTTCTTTAACGCTCATACTGCTCAGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCgn < 2:224493/150‑2 (MQ=255) cgagggtgtCGTCAACGCGCATACTGCTCAGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTg < 2:80884/142‑1 (MQ=255) gagaTTTTCGTTAACGCGCATACTGCTCAGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGc < 2:234429/150‑1 (MQ=255) gagaTGTTCGTTAACGCGCATACTGCTCAGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGc < 2:115972/150‑1 (MQ=255) gaTTTTCGTTAACGCGCATACTGCTCAGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCcg > 1:330717/1‑150 (MQ=255) cgttcgcgttcATACTGCTCAGGCTGCCCAGCGCCGCCACCAGCAAATCCTCCACCGCCCGCTAGCAATAGTGGCAAAACAGCAGGCATCCACCGTCAGCACAAGGATATTTCGCTAATTCGTCACGTAGGCGGCCGGTCGCCGCGCGGc < 1:213365/140‑1 (MQ=255) cGTTAACGCGCATACTGCTCAGGTCGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCcgcgcgtc < 1:262120/150‑3 (MQ=255) cGTTAACCCGCATACTGCTCAGGTTGCTCAGAACTCCCACCAACAAATACTCCACCGCTAGTTAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGc < 1:138631/150‑1 (MQ=255) gcgcATACTGCTCAGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGc < 2:330717/150‑1 (MQ=255) tCAGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCct > 1:98782/1‑150 (MQ=255) cAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAATAAACTATGATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTg < 2:351870/150‑1 (MQ=255) gAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCACGCCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGAc < 2:205226/150‑1 (MQ=255) acggCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGc < 2:74491/147‑1 (MQ=255) gCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGt < 1:58621/150‑1 (MQ=255) ccaccaGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTa > 1:352121/1‑150 (MQ=255) caccaGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTaa > 2:304528/1‑150 (MQ=255) caGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAgcg < 2:143261/150‑1 (MQ=255) aaTACCCCACCGCCAGACAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCg < 2:164062/150‑1 (MQ=255) ccACCGCCAGAGAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTgcg < 2:114339/150‑1 (MQ=255) ccGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCa > 2:150260/1‑150 (MQ=255) gATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCGGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCAc > 2:161905/1‑150 (MQ=255) gATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCGGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCAc > 2:161809/1‑150 (MQ=255) tGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTa < 2:77561/150‑1 (MQ=255) ggCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTaa > 2:339375/1‑150 (MQ=255) aCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCGGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGc < 1:161905/150‑1 (MQ=255) aCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCGGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGc < 1:161809/150‑1 (MQ=255) cACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGAAATTGAGGTCTCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTa > 2:350677/1‑150 (MQ=255) tCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTg < 2:192840/150‑1 (MQ=255) ccgcaaagcgtcatTTCGCTTATTCGTCACGCGGGCGGCCGGTTGCCGCGCGGCTAAATGTCAGGACGCCCCCTTCGCCCCGACCTTGTAAGCGCTGCGTCTGCTCCGGTTCTCTCCCGTCGCTAAACAAGCCGTAAGCTTTATTTTTgg < 1:72867/138‑1 (MQ=255) ttCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGACCCGa > 2:218960/1‑150 (MQ=255) ttCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGAACTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGACCCGa > 2:245413/1‑150 (MQ=255) cGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGACAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTGTAGACCCGATg > 2:129138/1‑150 (MQ=255) aGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGACCCGATGCAGTTCTGCCACCAtc < 1:202472/150‑1 (MQ=255) | TGGGTTGATGTCGAGATTTTCGTTAACGCGCATACTGCTCAGGTTGCTCAGAACTGCCACCAGCAAATACTCCACCGCCAGATAGCGATAGTGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGACCCGATGCAGTTCTGCCACCATC > CP009273/2277016‑2277313 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |