Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,390,178 A→C Y25S (TAT→TCT)  hfq → global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,390,1780AC98.3% 186.2 / NA 60Y25S (TAT→TCT) hfqglobal sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (34/24);  minor base T (0/1);  total (35/25)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAAC  >  CP009273/4390034‑4390323
                                                                                                                                                |                                                                                                                                                 
gaCGTATCGTGCGCACTTATTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGCAAAGAGAGCATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCCCTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTg                                                                                                                                               <  2:858596/149‑1 (MQ=255)
gaCGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTg                                                                                                                                               <  2:630792/149‑1 (MQ=255)
      tCGTGCGCAAGTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAAt                                                                                                                                         <  2:1078579/149‑1 (MQ=255)
         tGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATATTTACAAGATCCGTTACTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGTTGAATGGt                                                                                                                                      >  1:220237/1‑149 (MQ=255)
              aaTTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTaa                                                                                                                                 >  1:1078863/1‑149 (MQ=255)
                ttttttCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTGTTTGGTGAATGGTATTAAGc                                                                                                                               >  1:881786/1‑149 (MQ=255)
                  ttttCAGAATCGAAAGGTCCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCGGTTTCTATTTCTTTGGTGAATGGTATTAAGCTg                                                                                                                             <  2:733529/149‑1 (MQ=255)
                      cAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAg                                                                                                                         >  2:632999/1‑149 (MQ=255)
                         aaTCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGc                                                                                                                      >  1:304177/1‑149 (MQ=255)
                           tCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCaa                                                                                                                    >  1:209843/1‑149 (MQ=255)
                              aaaGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGGATTAAGCTGCAAGGGCAAATc                                                                                                                 >  1:155670/1‑149 (MQ=255)
                                aGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGa                                                                                                               >  1:17975/1‑149 (MQ=255)
                                   ttCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTc                                                                                                            >  1:1014549/1‑149 (MQ=255)
                                     cAACGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCtt                                                                                                          >  1:302850/1‑149 (MQ=255)
                                      aaaGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCttt                                                                                                         >  1:330993/1‑149 (MQ=255)
                                       agatACAAATAAGCATATAACGAAAAGAGAGAATTGCTAAGGGGTAATCTTTACAACGTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCtttt                                                                                                        <  1:1116136/146‑1 (MQ=255)
                                          tACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGAt                                                                                                     <  1:1083566/149‑1 (MQ=255)
                                            cAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGGAAATCGAGTCTTTTGATCa                                                                                                   >  1:378421/1‑149 (MQ=255)
                                               aTAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCCGtt                                                                                                >  2:565829/1‑149 (MQ=255)
                                                       ataAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAACCGAGTCTTTTGATCGGTTGGTCATcg                                                                                        >  1:287054/1‑148 (MQ=255)
                                                         aaGGAAAAGAGAGAATGGCTAAGGGACAGTCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTg                                                                                      >  2:645336/1‑149 (MQ=255)
                                                          aGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGt                                                                                     <  2:963737/149‑1 (MQ=255)
                                                            gAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTg                                                                                   >  2:866374/1‑149 (MQ=255)
                                                            gAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGGGATCCTGTTg                                                                                   >  2:370837/1‑149 (MQ=255)
                                                             aaaaGAGAGAATGGCTAAGGGGCAATCTTTACAAAATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGGTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGa                                                                                  >  2:582871/1‑149 (MQ=255)
                                                              aaaGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGaa                                                                                 >  2:836209/1‑149 (MQ=255)
                                                                 tggagaATGGCTAAGGGGCAATCTTCACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTTTTTGGTGAATGGTATGAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGGTCGTGATCCTGTTGaaaaa                                                                              <  2:841173/147‑1 (MQ=255)
                                                                  agagaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAac                                                                             >  2:165752/1‑149 (MQ=255)
                                                                     gaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACg                                                                          <  1:1057913/149‑1 (MQ=255)
                                                                          gCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAg                                                                     >  2:442326/1‑149 (MQ=255)
                                                                            tAAGGGTCAATCTTTACAAGTTCTGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGcc                                                                   <  2:542890/149‑1 (MQ=255)
                                                                            tAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCGGTTCGTGATCCTGTTGAAAAACACGGTCAGcc                                                                   >  2:859470/1‑149 (MQ=255)
                                                                                 ggCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTTGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGGTCCTGTTGAAAAACACGGTCAGCCAGATg                                                              >  2:920677/1‑149 (MQ=255)
                                                                                 ggCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATGCTGTTGAAAAACACGGGCAGCCAGCTg                                                              >  1:1054887/1‑149 (MQ=255)
                                                                                   cAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGt                                                            <  1:1099694/149‑1 (MQ=255)
                                                                                    aaTCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGtt                                                           <  2:767685/149‑1 (MQ=255)
                                                                                    aaTCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGtt                                                           <  2:489188/149‑1 (MQ=255)
                                                                                     aTCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTTAAAAACACGGCCACACAGAGGGttt                                                          >  1:135054/1‑149 (MQ=255)
                                                                                      tCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGCTGGTTTa                                                         >  2:351658/1‑149 (MQ=255)
                                                                                         ttACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACaa                                                      >  1:794342/1‑149 (MQ=255)
                                                                                             aaGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAc                                                  <  1:632999/149‑1 (MQ=255)
                                                                                                ttCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAcgcg                                               <  1:109259/148‑1 (MQ=255)
                                                                                                     ttCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTc                                          <  2:304177/149‑1 (MQ=255)
                                                                                                        cTGAACGCACTGCGTCGGGAACGTGTTCCAGTTCCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTAc                                       <  2:881786/149‑1 (MQ=255)
                                                                                                         tGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACt                                      <  2:17975/149‑1 (MQ=255)
                                                                                                            aCGCACTGCGTCGGGGACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACtgtt                                   <  2:912521/149‑1 (MQ=255)
                                                                                                               cACTGCGTCGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTc                                <  2:1078863/149‑1 (MQ=255)
                                                                                                                      actggaacacgtTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACACGCATCCAGAGGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGtctc                         >  2:377144/11‑149 (MQ=255)
                                                                                                                       cGGGAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCg                        >  2:410178/1‑149 (MQ=255)
                                                                                                                          gAACGTGTTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGccc                     >  2:707938/1‑149 (MQ=255)
                                                                                                                           aacgttgctctgTTTCTATTTCTTTGGGGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCg                    <  1:1026091/138‑1 (MQ=255)
                                                                                                                              gtgtTCCCGTTTNTATTTCTTTTGTGCATNGTATTAAGCTGCAAGGGCAGATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGtt                 <  2:135054/149‑1 (MQ=255)
                                                                                                                               tgtTCCAGGTTCAATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGttt                <  2:92965/149‑1 (MQ=255)
                                                                                                                                gtTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGCTtc               >  1:760307/1‑149 (MQ=255)
                                                                                                                                 ttCCAGTGTCTAGTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCTTTATCCTGTTGAAAAACACTGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTtct              <  1:440023/149‑1 (MQ=255)
                                                                                                                                    cAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCtcat           >  2:341376/1‑149 (MQ=255)
                                                                                                                                    cAGTTTCTATTTCTTGGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCGGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGattgtgat           >  1:834743/1‑144 (MQ=255)
                                                                                                                                     agttcttatttcttTGGTGAATTGTATTAAGCTGCAAGGGCAAAGCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCtcatc          <  1:147397/143‑1 (MQ=255)
                                                                                                                                     aGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTGACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCtcatc          <  1:223035/149‑1 (MQ=255)
                                                                                                                                       tttCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATcac        >  2:521790/1‑149 (MQ=255)
                                                                                                                                       tttCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATAAGTTCGTGATCCTGTTGAAACACACGGTCAGCCAGATGGTTTGCAACCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATcac        >  1:751876/1‑149 (MQ=255)
                                                                                                                                            atttctttGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTaa   <  2:685405/149‑1 (MQ=255)
                                                                                                                                            atttctttGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTaa   >  2:52262/1‑149 (MQ=255)
                                                                                                                                             tttctttGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCATTCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTaac  >  2:89785/1‑149 (MQ=255)
                                                                                                                                             tttctttGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTaac  >  1:601493/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
GACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAAC  >  CP009273/4390034‑4390323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑