Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	2,419,720	T→C	D183G (GAT→GGT)	hisJ ←	histidine/lysine/arginine/ornithine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,419,720	0	T	C	100.0%	232.9 / NA	73	D183G (GAT→GGT)	hisJ	histidine/lysine/arginine/ornithine transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/46);  total (27/46)

GCTTCGCGCAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTAC  >  CP009273/2419576‑2419862                                                                                                                                                 |                                                                                                                                               gcctCGCGCAGTTCGTTATCTTCTTTACCCCGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTg                                                                                                                                            <  1:654712/146‑1 (MQ=255)   ttCGCGCAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGa                                                                                                                                          >  2:549980/1‑149 (MQ=255)   ttCGCGCAGCTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGa                                                                                                                                          <  1:971445/149‑1 (MQ=255)      gcgcAGTTCGTTAGCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAAcg                                                                                                                                       <  2:173201/149‑1 (MQ=255)         cAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCgg                                                                                                                                    <  1:1073490/149‑1 (MQ=255)         cAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCgg                                                                                                                                    >  2:290662/1‑149 (MQ=255)         acgTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCGGGCAGCGACCTCACCCTGAAAcgcgc                                                                                                                                    >  1:1048429/3‑148 (MQ=255)           gTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCa                                                                                                                                  <  2:759230/149‑1 (MQ=255)             tCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATc                                                                                                                                <  2:318483/149‑1 (MQ=255)             ccgtaaccttcatTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATc                                                                                                                                <  2:472462/142‑1 (MQ=255)                ttATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAAt                                                                                                                             >  2:296595/1‑149 (MQ=255)                 tATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATa                                                                                                                            <  2:1139364/149‑1 (MQ=255)                  aTCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGACCACCGAATTTGTAATCTTTACCGCCAGGTTGGTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGTATAAATaa                                                                                                                           >  1:891131/1‑148 (MQ=255)                     ttcttTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCCATACGTc                                                                                                                        >  1:1117775/1‑149 (MQ=255)                     ttcttTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCCCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTc                                                                                                                        >  2:171310/1‑149 (MQ=255)                      tcttTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTcc                                                                                                                       <  1:985273/149‑1 (MQ=255)                      tcttTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTcc                                                                                                                       >  1:1040200/1‑149 (MQ=255)                       cttTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCg                                                                                                                      <  2:439324/149‑1 (MQ=255)                        tttACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACTTCCgt                                                                                                                     <  1:199079/149‑2 (MQ=255)                            cgcgcgCCCATGCCGGTCCCTACGCCAAATAGTCTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAgt                                                                                                                 <  1:571757/144‑1 (MQ=255)                                 gcgcaggCCGGTGCCGTCGCCAATCCGTTTTTCATCTTGAACAGACGGGCCACCGAATTTGGAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGACAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAgtcaggt                                                                                                            <  1:985691/143‑1 (MQ=255)                                 gCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATACTGAACAGACGGGCCACCGAATTTATAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCGgtccggg                                                                                                            >  1:813941/1‑148 (MQ=255)                                  cccATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAgtcaggtc                                                                                                           <  1:679503/149‑1 (MQ=255)                                   cctcgCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAAACGACGGGCCACCGAATTTTTAATCGTTACCGACAGGTTGTTTGGGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAgtcaggtca                                                                                                          <  1:401082/145‑1 (MQ=255)                                         cGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGTAGTCAGGTCAGAATaa                                                                                                    <  1:302885/149‑1 (MQ=255)                                         cGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATaa                                                                                                    >  2:994653/1‑149 (MQ=255)                                          ggTGCCTACGCCAGACAGTGTTTCATCTTGAACAGACGGGCCACCGCATTTGTAATCGTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCGCCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATaaa                                                                                                   <  1:841157/149‑1 (MQ=255)                                          ggTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATaaa                                                                                                   <  1:960859/149‑1 (MQ=255)                                          ggTCCCTACGCCAAACAGTTTTTCATCTTTAAAAGCCGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATaaa                                                                                                   <  2:527048/149‑1 (MQ=255)                                           gTCCCTACCACCAACAGTTTTTCCTCTTCACCAGACGCGCCACCGAATTTGTTCTCTTTACCGACAGGTTGTCTGAGTAACCCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAAt                                                                                                  <  2:30304/149‑1 (MQ=255)                                              ccTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAAtgtt                                                                                               <  2:587194/149‑1 (MQ=255)                                                 aCGCCAAACAGTTTTTCATCTTTAACTGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTc                                                                                            <  1:1091178/149‑1 (MQ=255)                                                     cAAACAGTTTTTCCTCTTTAACCGACGGGCCACCCAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAATCCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGc                                                                                        <  2:768604/149‑1 (MQ=38)                                                        aCAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTATAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGCCAGGTCAGAATAAATGTTGTCCTGcccc                                                                                     <  2:748656/149‑1 (MQ=255)                                                                   tCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACATACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGAc                                                                          >  1:248913/1‑149 (MQ=255)                                                                      ttAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGAt                                                                       <  2:858132/149‑1 (MQ=255)                                                                      ttAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGAt                                                                       <  2:523717/149‑1 (MQ=255)                                                                       caaCAGCCAGGCCAGCGAATTTGTGATCGTTACCGACAGGGTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCTCCCTGGAACGCGGCATCAATACGGCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGAtt                                                                      <  2:1133494/148‑1 (MQ=255)                                                                        aaCAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGAttt                                                                     >  2:684211/1‑149 (MQ=255)                                                                          cAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCa                                                                   <  2:167398/149‑1 (MQ=255)                                                                            gatgggCCACCGAATTTGTAATCTGTACCGACGGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAAt                                                                 <  1:769861/146‑1 (MQ=255)                                                                             aCGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATg                                                                >  1:196088/1‑149 (MQ=255)                                                                              cGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGc                                                               <  1:884139/149‑1 (MQ=255)                                                                                  ccACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTCCAATGCCttt                                                           >  2:799536/1‑149 (MQ=255)                                                                                       gAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCCTCAATACGTCCCGCAGTCAGCTCAGAATAAATGTCGTCCCGCCCCTGATCCGAGACGATTTCAATGCCTTTTGGTg                                                      >  2:802007/1‑149 (MQ=255)                                                                                        aaTTTGCAATCGTTCCCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCTTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTCATACGAGACGATTTCAATGCCTTTTGGTGc                                                     <  2:597108/149‑1 (MQ=255)                                                                                           ttGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCa                                                  >  1:406140/1‑149 (MQ=255)                                                                                           ttGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCa                                                  <  2:5123/149‑1 (MQ=255)                                                                                           ttGTAATCTTTACCGACAGGTTGTTTGAGAAAACCTTAGCTGGCAGCGACCTCACCCTGGAACACGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCa                                                  >  2:744569/1‑149 (MQ=255)                                                                                             gTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAAt                                                >  2:974830/1‑149 (MQ=255)                                                                                              tAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCCGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGcccgacg                                               >  2:449999/1‑145 (MQ=255)                                                                                                aTCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCAAATGtt                                             >  2:1059153/1‑149 (MQ=255)                                                                                                aTCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCAGCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGAGTTCAATGCCTTTTGGTGCCCAATGtt                                             >  2:327505/1‑149 (MQ=255)                                                                                                 tCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTc                                            <  2:1040200/149‑1 (MQ=255)                                                                                                      aCCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTAc                                       <  1:940526/149‑1 (MQ=255)                                                                                                        cgtgaGGTTGTTTGAAGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGACATCAATACGTCCGGAAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAAACGATTTCAGTGCCTTTAGGTGCCCACTGTTCATTGcca                                     >  1:32883/5‑148 (MQ=255)                                                                                                                    tGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTgg                         <  2:531183/149‑1 (MQ=255)                                                                                                                    tGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAAGACGTCCGGCAGTCAGGTCACACTAAATGCTGGCCGGCCCCTGAGACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTgg                         <  1:290662/149‑1 (MQ=255)                                                                                                                      aGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTAAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGggt                       <  2:794884/149‑1 (MQ=255)                                                                                                                        gAATCCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGGTTTCAATGGCTTTTGGTGCCCAATGTTCATTGGCGTACGTGTTCTGggtgg                     >  1:730371/1‑149 (MQ=255)                                                                                                                          aaCCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGgtggtg                   >  1:408417/1‑149 (MQ=255)                                                                                                                           aCCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGc                  <  2:447073/149‑1 (MQ=255)                                                                                                                           aCCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGc                  >  2:470003/1‑149 (MQ=255)                                                                                                                            ccTTCGCTGGCAGCGCCCTCACCCTGGAACGCGGCATCATTACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGcc                 <  2:813941/149‑1 (MQ=255)                                                                                                                            ccTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGcc                 <  2:1009405/149‑1 (MQ=255)                                                                                                                             cTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCCCCTGGGTGGGGccc                >  1:1025568/1‑149 (MQ=255)                                                                                                                              ttCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCCTTACCGAACGTCTCCCGGGTGGTCCCCt               >  1:577232/1‑149 (MQ=255)                                                                                                                               tCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTg              <  1:1062707/149‑1 (MQ=255)                                                                                                                               tCGCGGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTg              <  2:375622/149‑1 (MQ=255)                                                                                                                                 gCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCa            <  1:684211/149‑1 (MQ=255)                                                                                                                                  cTGGCAGCGACCTAACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCaa           <  2:527884/149‑1 (MQ=255)                                                                                                                                     gCAGCGACCTCCCCCGGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCCGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATAc        <  2:198475/149‑1 (MQ=255)                                                                                                                                           cccTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTAc  <  1:655877/148‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                               GCTTCGCGCAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTAC  >  CP009273/2419576‑2419862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑