mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.32.0b
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | A8_F017_R2_F16_I30_S2161_L001_R2_001.good.fq | 1,143,658 | 170,275,778 | 100.0% | 148.9 bases | 149 bases | 94.7% |
| errors | A8_F017_R2_F16_I30_S2161_L001_R1_001.good.fq | 1,143,658 | 170,275,778 | 100.0% | 148.9 bases | 149 bases | 98.5% |
| total | 2,287,316 | 340,551,556 | 100.0% | 148.9 bases | 149 bases | 96.6% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | CP009273 | 4,631,469 | 70.3 | 1.9 | 100.0% | Escherichia coli BW25113, complete genome. |
| total | 4,631,469 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 14955 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 312 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.020 |
| reference sequence | pr(no read start) |
|---|---|
| CP009273 | 0.81829 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.8 |
| Consensus minimum coverage each strand | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum coverage each strand | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.3.2 |
| R | 3.4.2 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 21:03:55 15 Jun 2018 | 21:04:23 15 Jun 2018 | 28 seconds |
| Read alignment to reference genome | 21:04:23 15 Jun 2018 | 21:06:38 15 Jun 2018 | 2 minutes 15 seconds |
| Preprocessing alignments for candidate junction identification | 21:06:38 15 Jun 2018 | 21:07:02 15 Jun 2018 | 24 seconds |
| Preliminary analysis of coverage distribution | 21:07:02 15 Jun 2018 | 21:08:08 15 Jun 2018 | 1 minute 6 seconds |
| Identifying junction candidates | 21:08:08 15 Jun 2018 | 21:08:12 15 Jun 2018 | 4 seconds |
| Re-alignment to junction candidates | 21:08:12 15 Jun 2018 | 21:08:38 15 Jun 2018 | 26 seconds |
| Resolving alignments with junction candidates | 21:08:38 15 Jun 2018 | 21:09:17 15 Jun 2018 | 39 seconds |
| Creating BAM files | 21:09:17 15 Jun 2018 | 21:10:24 15 Jun 2018 | 1 minute 7 seconds |
| Tabulating error counts | 21:10:24 15 Jun 2018 | 21:10:52 15 Jun 2018 | 28 seconds |
| Re-calibrating base error rates | 21:10:52 15 Jun 2018 | 21:10:53 15 Jun 2018 | 1 second |
| Examining read alignment evidence | 21:10:53 15 Jun 2018 | 21:16:42 15 Jun 2018 | 5 minutes 49 seconds |
| Polymorphism statistics | 21:16:42 15 Jun 2018 | 21:16:42 15 Jun 2018 | 0 seconds |
| Output | 21:16:42 15 Jun 2018 | 21:16:52 15 Jun 2018 | 10 seconds |
| Total | 12 minutes 57 seconds | ||
