Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC CP009273 2,400,257 IS5 (+) +4 bp 50.8% intergenic (‑137/‑780) lrhA ← / → alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 2282398 =NA (NA)10 (0.350) 9/270 NT 39.3% noncoding (1195/1195 nt) IS5 repeat region
?CP009273 2400257 = 15 (0.540)intergenic (‑137/‑783) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2
* ? CP009273 = 2283592NA (NA)21 (0.730) 18/270 NT 57.6% noncoding (1/1195 nt) IS5 repeat region
?CP009273 = 2400260 15 (0.540)intergenic (‑140/‑780) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

CAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAA  >  CP009273/2282250‑2282526
                                                                                                                                                    |                                                                                                                                
cAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCCTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATaag                                                                                                                                  <  1:41952/149‑1 (MQ=255)
          tataTGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGa                                                                                                                        <  1:366035/149‑1 (MQ=255)
                  gTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCgg                                                                                                                <  1:327322/149‑1 (MQ=255)
                               attgattTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAAGTGCGAATAAGCGGGGaagtg                                                                                                         >  2:290144/1‑140 (MQ=255)
                                  gattTTCGTGTTAGCGGCGGTCCCATACACCAGCTGGATATCTTTTTCCAGGACAACACCCTCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGc                                                                                                <  1:255172/149‑1 (MQ=39)
                                        cGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTc                                                                                          <  1:440647/149‑1 (MQ=255)
                                          tattatTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGAGTCAg                                                                                        >  1:227632/1‑149 (MQ=255)
                                                  gCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtc                                                                                      <  2:29584/143‑1 (MQ=255)
                                                                           cTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAAAGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCCTTTCTTCATGTTTGAGCCGAttttt                                                       >  1:131794/1‑149 (MQ=255)
                                                                            tttttCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGGGCCGa                                                            >  2:327907/1‑143 (MQ=255)
                                                                             tttgCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGACGCCAATGCTATGGGTGAATATAAGGTAGGTGCGAATAAGCGGGGAAATTCTACTCGGCTGTCTCAGTCATCTCATTTCTTCATGTTTGAGCCGATTTTTtc                                                     <  1:390263/149‑1 (MQ=25)
                                                                                   aGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCCTCATGTTTGAGCCGATTTTTtc                                                     >  2:116309/1‑143 (MQ=255)
                                                                                     gACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAAGATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtctc                                                   >  2:234210/1‑143 (MQ=255)
                                                                                       caactCCATCAACGGTCTCACCAGCCTCAACAGTGGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATATGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTccc                                                 <  2:428400/143‑1 (MQ=18)
                                                                                           acCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATACGGACGGTGTGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCACTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCTCGTaa                                             >  2:71258/1‑143 (MQ=21)
                                                                                            ccATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTGATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCtt                                      >  1:51934/1‑149 (MQ=255)
                                                                                            ccATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTaaa                                            <  2:300818/143‑1 (MQ=255)
                                                                                                  aCTGTCTCACCATACTCACCAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCtt                                      <  2:98687/143‑1 (MQ=255)
                                                                                                  aCTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCa                                <  1:327158/149‑1 (MQ=255)
                                                                                                    tGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCCCCCGTAACTGCCTTgg                                    >  2:118553/1‑142 (MQ=255)
                                                                                                        tcACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCa                                <  2:136156/143‑1 (MQ=255)
                                                                                                          aCCAGACTCAACGGTTGATGCCAATGTTATGGGTGAATATAAGGAAGTTGCGATTAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGc                              <  2:196495/143‑1 (MQ=17)
                                                                                                              tACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGGGCCGCTTTTTTCTCCCGTAAATGGCTTGAATGAGCCTATTTCGAc                    >  1:34187/1‑149 (MQ=18)
                                                                                                                 tCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGt                 >  1:418413/1‑149 (MQ=255)
                                                                                                                 ccAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTa                       <  2:40714/142‑1 (MQ=255)
                                                                                                                    aCAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGCttc              >  1:85908/1‑149 (MQ=255)
                                                                                                                         tGATGCCAACGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAGTTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGc         <  1:147831/149‑1 (MQ=255)
                                                                                                                          gATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTACCGTAAATTCCTTGAATCAGCCTATTTAGACCGTTTCTTCGcc        >  1:99034/1‑149 (MQ=255)
                                                                                                                             ccccatgcattGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTttcttc           >  2:437115/11‑143 (MQ=255)
                                                                                                                               cAATGCTATTGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGc         <  2:141884/143‑1 (MQ=255)
                                                                                                                                aaTGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTaa  >  1:420856/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                
CAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAA  >  CP009273/2282250‑2282526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.