Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	CP009273	2,420,338:1	+AATT	51.8%	intergenic (‑71/+150)	hisJ ← / ← argT	histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	= 2420334	17 (0.610)	18 (0.650) +AATT	16/254	NT	51.8%	intergenic (‑67/+154)	hisJ/argT	histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit
	?	CP009273	2420335 =	17 (0.590)					intergenic (‑68/+153)	hisJ/argT	histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit

ATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACA  >  CP009273/2420197‑2420471                                                                                                                                           |                                                                                                                                         cagcaaacgccgcagttgcgctggagaaggccagaaccagagagagcggtagcaccagttttttcataatgtcctcaactgacagtctttttatagggaatagttacaggtttgttacacttatcgtaacataaaattAATTGACCATc                                                                                                                                >  1:275081‑M2/139‑149 (MQ=255)   gcaaacgccgcagttgcgctggagaaggccagaaccagagagagcgatagcaccagttttttcataatgtcctcaactgacagtctttttatagggaatatttacaggtttgttacacttatcgtaacataaaattAATTGACCATCaa                                                                                                                              <  1:314357‑M2/13‑1 (MQ=255)         gccgcagttgcgctggagaaggccagaaccagagagagcgatagcaccagttttttcatcatgtcctcaactgacagtctttttatagggaatatttacaggcttgctacacttatcgtaaaataaaattAATTGACCatcga                                                                                                                              >  2:449931‑M2/131‑141 (MQ=255)         gccgcagttgcgctggagaaggccagaaccagagagagcgatagcaccagttttttcataatgtcctcaactgacagtctttttatagggaatatttacaggtttgttacacttatcgtaacataaaattAATTGAGCATCaa                                                                                                                              >  2:124444‑M2/131‑143 (MQ=255)          aaCGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCCCCAGTTGTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGcgaca                                                                                                                       >  1:79105/1‑145 (MQ=255)               cgcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACCCTTATCGTGCCATAAAATTGCCCATCAAGGCaca                                                                                                                        >  2:390491/1‑141 (MQ=255)                   cgctggagaaggccagaaccagagagagcgatagcaccagttttttcataatgtcctcaactgacagtctttttatagggaatatttacaggtttgttacacttatcgtaacataaaattAATTGACCATCAAGGCAAAATATCCCGcc                                                                                                              >  1:305916‑M2/121‑149 (MQ=255)                    gctgggaaaggtcagcaccagagagagctataccaccagtttttgcataatgtcctcaactgacagtctttttatagggaatatttacaggtttgttacacttatcgtaacataaaattAATTAACCATCAAGGCAaaatacc                                                                                                                   <  2:411349‑M2/24‑3 (MQ=255)                       ggagaaggccagaaccagagagagcgatagcaccagttttttcataatgtcctcaactgacagtctttttatagggaatatttacaggtttgttacacttatcgtaacataaaattAATTGACCATCAAGGCAAAATATCCCGCCCCGt                                                                                                          >  1:525408‑M2/117‑149 (MQ=255)                         cTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGcc                                                                                                              >  2:444633/1‑143 (MQ=255)                          tGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAg                                                                                                       >  1:406440/1‑149 (MQ=255)                           ggAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGa                                                                                                      >  1:413540/1‑149 (MQ=255)                                aGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCTACTTACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGtat                                                                                                 <  1:30790/149‑1 (MQ=255)                                    accagagagagcgatatcaccagttttttcataatgtcctcaactgacagtctttttatagggaatatttacaggtttgttacacttatcgtaacataaaattAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTattatt                                                                                             <  1:78480‑M2/46‑1 (MQ=255)                                     ccagagagagcgttagcaccagttttttctttatgtcctcaactggcagtctttttataacgaatatttacaggtttgttacacttatcgtaacataaaattAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTAttt                                                                                            <  1:259877‑M2/47‑1 (MQ=255)                                         ccAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGtattat                                                                                              >  2:86711/1‑143 (MQ=255)                                               agagCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCa                                                                                        <  2:17882/143‑1 (MQ=255)                                               agacCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTACCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCa                                                                                        <  2:339781/143‑1 (MQ=255)                                                       aGCACCATTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTaa                                                                          <  1:397120/149‑1 (MQ=255)                                                                     cATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTCTGCATTTGAAt                                                            >  1:129427/1‑149 (MQ=255)                                                                     cATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCACAAAATTGACCATCAATGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATACTTAAGTATGCATTTGAAt                                                            >  1:318997/1‑149 (MQ=255)                                                                            tCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAACGCTTAAGTATGCATTTAAATg                                                           >  2:217060/1‑143 (MQ=255)                                                                                 gacagtctttttatagggaatatttacaggtttgttacacttatcgtaacataaaattAATTGACCATCAAGGCGAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAACATGCTTACGTATGAATTTCcatgcccaa                                                      >  2:129255‑M2/59‑139 (MQ=255)                                                                                            tatagggaatatttacaggtttgttacacttatcgtaacataaaattAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATCGTGcaa                                           >  2:454441‑M2/48‑143 (MQ=255)                                                                                                 ggaatatttacaggttagttgcacttatcgtaacataaaattAATTGACCATCAAGGCAAAGTATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAAc                                      >  2:333787‑M2/43‑143 (MQ=255)                                                                                                      gAATATTTACAGGTTTGCTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGc                                 <  2:294257/143‑1 (MQ=255)                                                                                                           tttACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCa                            >  2:377532/1‑143 (MQ=255)                                                                                                           caggtttgtgacccttatcgtaacataaaattAATAGACCATCAAGGCAAAATCTCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCatga                      <  1:397017‑M2/117‑1 (MQ=255)                                                                                                            aggtttgttacacttatcgtaacataaaattAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCTTTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGCCGTGCACCatgat                     <  1:361877‑M2/118‑1 (MQ=255)                                                                                                              gtttctcacacttatcgtaacataaaattAATGGCCCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTACAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCa                         <  2:426756‑M2/114‑1 (MQ=255)                                                                                                                   tttGTTACACTTATCAGGCCATAAAAGTGACCATCAATGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCAtt              <  1:302645/149‑1 (MQ=255)                                                                                                                   ttacacttatcgtaacataaaattAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCatgatg                    <  2:270891‑M2/119‑1 (MQ=255)                                                                                                                    tacacttatcgtaacataaaattAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCAtgatga                   <  2:475695‑M2/120‑1 (MQ=255)                                                                                                                         ttatcgtaatataaaattAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCAtt              <  2:213782‑M2/125‑1 (MQ=255)                                                                                                                               taacataaaattAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCCTTCCATCa        >  2:197448‑M2/13‑143 (MQ=255)                                                                                                                               ctCGTGCCATAAATATTACCATCAAGGCAAAATTTCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATCCATTTCAATGCACTACGATAGTGCAACAAACCTGGCGAGCACCATGATGATCATTCCATCAGGTACa  <  1:322059/148‑1 (MQ=255)                                                                                                                                           |                                                                                                                                         ATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACA  >  CP009273/2420197‑2420471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.