| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,969,337 | G→T | 49.7% | E278* (GAG→TAG) | wecE → | TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,969,337 | 0 | G | T | 49.7% | ‑2.5 / 39.7 | 34 | E278* (GAG→TAG) | wecE | TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase |
| Reads supporting (aligned to +/- strand): ref base G (11/6); new base T (8/8); total (19/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.60e-01 | |||||||||||
TGGCTCCAGCTATTTGATGTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTACATTCCGCTGCA > CP009273/3969192‑3969479 | tGGCTCCAGCTATTTGATGTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGc < 1:322931/149‑1 (MQ=255) cTCCAGCTATTTGATGTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGc > 1:403079/1‑149 (MQ=255) cgcgagttGATGTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCt < 2:280899/137‑1 (MQ=255) aTTTGATGTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTCTCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGAtt < 1:385830/149‑1 (MQ=255) tGATGTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCg > 2:109227/1‑143 (MQ=255) tGTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGAt < 1:182210/149‑1 (MQ=255) gTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGTCGTCGATTc > 2:430022/1‑143 (MQ=255) tCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGCCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGCTTcc > 2:399713/1‑143 (MQ=255) cGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAAGGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGCAAGCCGGGCGTATCGAGCTGCCATCGATTCCCg > 2:247737/1‑143 (MQ=255) cGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGCTGGCt > 1:318970/1‑149 (MQ=255) cGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCt > 1:318934/1‑149 (MQ=255) cAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCa > 1:501425/1‑149 (MQ=255) tgGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGtt > 1:396470/1‑149 (MQ=255) gcgcAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTa < 1:477880/149‑1 (MQ=255) gcgcAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTAATACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTa > 1:521603/1‑149 (MQ=255) aactcaaaGCAGCGCATCGAATCAACCAGCAACGCCTGGCGCTGTGGCAAAACCACTACGATGCGTTACCCCCTCCGCCGAAACCCGGGCCTACCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGtcc < 2:433528/137‑3 (MQ=255) aCTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTa < 1:78278/149‑1 (MQ=255) ctgccgcatatgataaCTAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATTCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATAAGTTCTACATTcaa > 2:4372/15‑143 (MQ=255) cagcGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTCCTCCGGCGCATGGTCGCCTCTGGCGATTGCTGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCgg < 1:232147/149‑1 (MQ=255) cGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTg > 2:416242/1‑143 (MQ=255) tATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATtgatg > 1:34383/1‑149 (MQ=255) tCAACCAGCAACGCCGGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGACAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGGACGCGCATATGTGCTACAGTAAAATGCGGGCTAtt > 2:260108/1‑143 (MQ=255) cAACCAGCAACGTCTGGCGCGGTGGCAAAACTACTACGATGCGTGAGCGTCTCTGGCGAAAGGCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATtg < 2:389937/143‑1 (MQ=255) cAGCAACGTCTGGCGCTGGGGCGAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATtgatga < 2:252065/143‑1 (MQ=255) gcTGGGGCAAAACCACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGa < 2:79520/143‑1 (MQ=255) gCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGa > 1:166041/1‑149 (MQ=255) gCAAAACTACTACGATGCGTTAGCGCCTCAGGCGAAAGCCGGGCGTATCGAGCAGCCGTCGATTCCCCATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGATCGGAGCGCGTAGATTAACTTTCTGa > 1:322036/1‑149 (MQ=255) gATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGCTTAACTTTGTGAAAGAAGCGGAAAt > 1:396079/1‑149 (MQ=255) tGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCTAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAAGCGGAAATCa > 1:79392/1‑149 (MQ=255) tAGCGCCCCTGGCGAAAGCCGGGTGTATCTAGCTGCCGTCGTTTCATGGATGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAAGCGGAAATCATGGCg < 1:464780/149‑1 (MQ=255) ctctGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGGGCGCGTTGATTAACTTTTTGAAAGATGCGGagttcgtggag > 2:230834/1‑133 (MQ=255) gCGAAAGCCGGGCGTATCCAGCTGCCGCCGATTCCCGAGGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTa < 1:2127/149‑1 (MQ=255) cgCCGGGCGTCTCGAGCAGCCGTCGAGTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAACGAAGCGGAAATCATGGCGGTGTTTCATTACATTc < 1:278991/148‑1 (MQ=255) cGTAGCGAGCTGCCGTCGATTCCCGATGGCGGCCTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTACATTCCGCTGCa < 1:70978/149‑1 (MQ=255) | TGGCTCCAGCTATTTGATGTCCGATCTGCAAGCTGCATACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGCGTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCTACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCATTACATTCCGCTGCA > CP009273/3969192‑3969479 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |